Seawater samples were collected using an epoxy coated CTD-rosette mounted with Go-Flo samplers and a Sea-Bird Electronics CTD (SBE9plus). Go-Flo bottles were transferred to a trace metal clean van for subsampling into polypropylene tubes (nutrients), polypropylene bottle (biogenic silica and particulate carbon and nitrogen) or TM acid-cleaned polycarbonate incubation bottles (Si-32 & C-14 incubation experiments).
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Nutrient samples were filtered through 0.2 \u03bcm polycarbonate filters and frozen at -20\u00b0C. Samples for biogenic silica concentrations were size fractionated by serial filtration through 5 \u03bcm and 0.6 \u03bcm polycarbonate filters. Filters were stored frozen at -20\u00b0C. Particulate organic carbon and nitrogen were measured on samples from experiments examining the effect of added Fe and Si on carbon fixation. These samples were filtered through precombusted GFF filters placed in glass scintillation vials and frozen at -20\u00b0C.
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Samples for silicic acid uptake profiles were spiked with the radioisotope Si-32. Nutrient limitation assays were performed on pairs of samples where rate of silicic acid uptake (Si-32) or carbon fixation (C-14 in paired light/dark bottles) were determined in unaltered controlled samples and in samples augmented with either silicic acid (20 \u03bcM) or iron chloride (1 nM). All samples were incubated on deck in simulated in situ incubators cooled with flowing surface seawater from 24 h. Profiles samples six depths from near surface to the 1% light level. Nutrient limitation assays were performed at the 40% and 10% light levels.
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Particles from incubated samples were size fractionated by serial filtration through 5 \u03bcm and 0.6 \u03bcm 25 mm polycarbonate filters. For C-14 incubations, total radioactivity in each sample was determined by sampling 100 \u03bcl of sample seawater prior to filtration. Filters from Si-32 incubations were placed on plastic planchettes and dried before covering with mylar film and stored or analysis ashore using low level beta counters (Riso Inc). Filters from C-14 incubations were acidified in glass scintillation vials, scintillation cocktail (Ultima Gold XR) added followed by liquid scintillation counting. Total radioactivity samples received 100 \u03bcL of b-phenethylamine and 5 mL of scintillation cocktail prior to analysis at sea using a Beckman 8500 scintillation counter.
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For more information, see the Protocol documents (under Supplemental Files).
Depth profiles in the euphotic zone of nutrient (nitrate, silicate, phosphate) concentrations, profiles of silicic acid uptake rates and assessment of limitation by Si and Fe on both silicic acid uptake and carbon fixation.
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See related dataset:\u00a0https://www.bco-dmo.org/dataset/786013
Silicon uptake was calculated as the product of the fraction of total Si-32 radioactivity taken up and the ambient silicic acid concertation. Rates of primary production were calculated as the product of the fraction of total C-14 radioactivity taken up and a DIC value of 2132 \u03bcmol kg-1 correcting for isotope discrimination (x 1.05).
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Nutrient\u00a0concentrations were adjusted using certified JAMSTEC CRMs.
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BCO-DMO Processing:
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