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Seawater samples for pigment analyses were immediately filtered (2 to 5 l volume) onto Whatman 47 mm GF/F filters under low vacuum (<0.5 atm). The filters were blotted dry, and stored in 2 ml cryotubes (Fisher) in liquid nitrogen until analysis. Analyses were performed within 6 months to a year of sampling.

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Extraction procedures and HPLC analytical methods follow those described in Thomas (2012) and HPLC_Method_Summary.pdf (see related files and publications).\u00a0

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High pressure liquid chromatography analyses of photosynthetic pigments taken on the R/V Acadian and R/V Pelican from September to October 2017 in the Central northern Gulf of Mexico.

\n

These data have been published in NASA SeaBASS Data Archive:

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HPLC samples were analyzed during August-September 2018 at the Horn Point Analytical Laboratory by Meg Maddox (mmaddox@umces.edu). Values of -8888 indicate concentrations that were below detection levels. Values of -9999 indicate missing data. Coefficient of variation (replicate filter precision) for all NSF_Gulf_Rapid_HPLC samples was TChl a = 3.09%; Ppig = 4.28%.

\n

The HPLC was controlled by Agilent Chemstation software.

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