Fragments of Orbicella faveolata, Porites astreoides were collected from Little Grecian reef (25.1193 N 80.3008 W) and Siderastrea radians was collected from Florida Bay (25.1017 N, 80.4391 W) in Key Largo, FL, USA, in August of 2013 as permitted by the Florida Keys National Marine Sanctuary (FKNMS-2011-093, FKNMS-2014-015). Exposed skeleton was covered with modeling clay, and the colonies were maintained in closed circulation tanks filled with reef seawater, allowing at least 2 days of recovery after collection prior to experimentation. The tank was exposed to a natural light regime, with shading added at midday to keep solar irradiance below 600 umol photons m\u22122 s\u22121.
Coral external carbonic anhydrase (CA) activity was measured on each fragment using an 18O-exchange technique described in (Tansik et al. 2015). Coral tissue was then removed using an air-brush filled with artificial seawater. The coral-algal slurry was homogenized and then centrifuged to remove symbiotic algae. A portion of the purified homogenate was then assessed for total CA activity using an 18O-exchange approach. Internal CA activity was determined by subtracting external CA activity from total CA activity. All 18O-exchange data was processed using MATLAB code, which is publicly available at https://github.com/bmhopkinson/18O_processing. Methodological details are described in Hopkinson et al. (2015).
\nNote:
\nBrian Hopkinson's github repo has been forked by BCO-DMO to ensure a copy of the code is available. This can be accessed at\u00a0https://github.com/BCODMO/18O_processing. A .zip file of the code is also attached to this dataset as a Supplemental File.
BCO-DMO Processing:
\n- modified columnn names;
\n- added columns for: Latitude, Longitude, Collection_Date;
\n- forked original github repo and downloaded a .zip file of the code.