Thalassiosira pseudonana were removed from the co-cultures by pre-filtration through 2.0 \u00b5m pore-size filters, and bacteria were collected on 0.2 \u00b5m pore-size filters. Filters were incubated in SDS (0.6% final concentration) and proteinase K (120 ng \u03bcl \u20131 final concentration). RNA was extracted from duplicates of each treatment by adding an equal volume of acid phenol:chloroform:isoamyl-alcohol, followed by shaking, centrifugation, and collection of the supernatant. A second extraction was carried out by the addition of an equal volume of chloroform:isoamyl-alcohol. RNA was recovered from the supernatant, treated to remove rRNA, and sequenced.
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Each sequence library is from four independent bacterial samples that were pooled for sequencing. The data are assigned to one of the four samples post-sequencing by mapping to the genomes of each of the four bacteria. The data table provides the accession numbers of the bacterial genomes to be used for mapping in the final column.
Transcriptome data for bacteria Ruegeria pomeroyi DSS-3, Stenotrophomonas sp. SKA14, Polaribacter dokdonensis MED152, and Dokdonia MED134 collected eight hours after individual inoculation into a diatom Thalassiosira psuedonana culture. The sequence data description for PRHNA448168 is at https://www.ncbi.nlm.nih.gov/bioproject/?term=PRJNA448168.
BCO-DMO Processing Notes:
\n- data submitted in Excel file \"Diatom matrix data table.xlsx\" sheet \"Sheet1\" extracted to csv
\n- added conventional header with dataset name, PI name, version date
\n-\u00a0NCBI_Genome_Accession data were split into separate rows
\n- Replicate number and\u00a0Taxon were put into their own columns
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