Dataset: HPLC_pigments
Deployment: KIWI9

   PI:              Ralf Goericke
   of:              University of California, San Diego
   dataset:         Pigments, HPLC method, standard casts
   dates:           February 16, 1998 to March 14, 1998
   location:        N: -52.9662  S: -71.3072  W: -174.7325  E: -165.9148
   project/cruise:  AESOPS/RR_KIWI_9, APFZ Polar Front Process 2
   ship:            R/V Roger A. Revelle
 
   Methodology:
   Samples that had been frozen in liquid nitrogen were extracted as described
   previously (Goericke and Repeta, 1993, MEPS 101: 307 - 313).
   Pigment extracts were analyzed on the reverse phase HPLC system described
   below.   Pigments were detected by absorbance at 440nm.  The identity of
   pigments was confirmed by frequently checking on-line spectra against those
   of standards.
 
   Column - Alltech Adsorbosphere C18, HS 3um, 4.6 mm, 10 cm.
   Solvents - A [methanol : acetonitrile : water : 0.5M aq ammonium acetate
   (30:30:30:10)
   B [methanol : acetonitrile : ethyl acetate (10:35:55)].
   Gradient - [time (min); solvent A, solvent B] - [0; 100, 0],   [4; 75, 25],
   [24; 12, 88], [26; 12, 88], [28; 100, 0].
   Instruments - Waters 510 pumps, Shimadzu autosampler and uv/vis detector,
   Waters 991M photodiode detector and Waters Millenium data system