Dataset: Emerald Reef Seasonal Monitoring, 2014-2015
Deployment: Baker_lab_2015

Coral tissue biopsies were taken from replicate, one-inch cores of Montastraea cavernosa as small scrapings along a polyp’s calyx wall using a razor blade. The samples were immediately placed into 300uL of 1% SDS in DNA Buffer in 1.5mL Eppendorf tubes and heated to 65°C for one hour, where they are now stable at room temperature. Small aliquots of this lysate are then removed for DNA extraction on as as-needed basis. 

We retain archived lysates (consisting of SDS-lysed tissue and a small piece of skeleton) that are stable long-term (>18 years) at room temperature.  

Data for this project will ultimately include symbiont:host cell ratios for these samples using a variety of Symbiodinium clade-specific probes. In addition, data may also include the results of ITS2 genotyping using Denaturing Gradient Gel Electrophoresis (DGGE). Additional data, such as environmental data collected by dataloggers during experiments or field deployments, may also be contributed.