Dataset: limpet aerial respiration
Deployment: Denny_2013

Individual aerial respiration rate data for limpets from trials

Principal Investigator:

Dr Mark W Denny (Stanford University - Hopkins, Stanford-HMS)

Co-Principal Investigator:

Dr Bengt J Allen (California State University Long Beach, CSULB)

Contact:

Luke P. Miller (Stanford University - Hopkins, Stanford-HMS)

BCO-DMO Data Manager:

Nancy Copley (Woods Hole Oceanographic Institution, WHOI BCO-DMO)

Project:

Environmental Variability, Functional Redundancy, and the Maintenance of Ecological Processes: Experiments in a Model Ecosystem (Experiments in a Model Ecosystem)

Current State:

Final no updates expected

Version:

2016-01-13

Version Date:

2016-01-13

Data URL:

https://www.bco-dmo.org/dataset-deployment/630123/data

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Description

Growth data for animals on experimental plates in the field during 2013 was collected monthly via digital photographs and measured using ImageJ software. Respiration of limpets collected from the field during summer 2013 was measured in air or seawater at a range of temperatures for one hour or two hours.

Related Reference:

Miller, L.P., B.J. Allen, F.A. King, D.R. Chilin, V.M. Reynoso and M.W. Denny (2015). Warm microhabitats drive both increased respiration and growth rates of intertidal consumers. Marine Ecology Progress Series 522: 127-143 doi: http://dx.doi.org/10.3354/meps11117

Related Datasets:

limpet aquatic respiration
limpet mass and body volume
limpet R-code and images

These data are also available at the Stanford Digital Repository: https://purl.stanford.edu/mz343tz6255

Methods & Sampling

Dataset acquisition description

Oxygen measurements taken using Ocean Optics FOXY fluorescence-based optode. Limpet mass data for all measured animals is included. Detailed methodology is available in Miller et al (2015).

Data Processing Description

Dataset Processing Description

Growth measurements were made by analyzing limpet shell projected area in ImageJ. Limpet respiration time series were used to estimate oxygen consumption rate. Complete analysis for size measurements and derived respiration rates are provided in the attached R code.

BCO-DMO Processing:

- added conventional header with dataset name, PI name, version date
- renamed parameters to BCO-DMO standard
- replaced space with underscore

More information about this dataset deployment

Funding

Award Number	Funding Source
OCE-1130095	NSF Division of Ocean Sciences
OCE-1131038	NSF Division of Ocean Sciences

Instruments

Oxygen Sensor

Supplied Name: ruthenium sensor spots

Supplied Description:

SP-PSt3-NAU-D5-YOP, PreSens Precision Sensing

Instrument Type

Generic Name: Oxygen Sensor

Acronym: Dissolved Oxygen Sensor

Generic Description:

An electronic device that measures the proportion of oxygen (O2) in the gas or liquid being analyzed

Water Temperature Sensor

Supplied Name:

Supplied Description:

iButton temperature logger (DS1921G, Maxim Integrated)

Instrument Type

Generic Name: Water Temperature Sensor

Acronym: Water Temp Sensor

Community Identifier: http://vocab.nerc.ac.uk/collection/L05/current/134/

Generic Description:

General term for an instrument that measures the temperature of the water with which it is in contact (thermometer).

Parameters

Supplied Name	Supplied description	Supplied Units	Standard Name
species	Lottia species name	unitless	species
limpet	unique identifying label for each limpet consisting of a 3-character species name abbreviation and a 3 or 4 digit number: lim = Lottia limatula; dig = L. austrodigitalis; sca = L. scabra; pel = L. pelta	unitless	sample
well	record of the individual respiration chamber well the limpet was in; values can be 1-15	unitless	no_bcodmo_term
temp	temperature of the water bath (and respiration chamber)	degrees Celsius	temp
dry_wt_limpet_g	limpet tissue dry mass after drying in the oven for 48hrs; the tissue was dissected from the shell	grams	dry_wgt_globec
rate_blank	average rate of change of oxygen concentration in the 3 blank chamber wells used in each trial. The same value is used to correct the respiration rates for all limpets in a trial run.	umol oxygen/hour	resp
rate_raw	raw uncorrected oxygen consumption rate of the limpet. Originally derived by fitting a linear regression to the individual time points for which oxygen was measured in the chamber during the run; converted to umol O2. NOTE: negative values indicate oxygen consumption and lower numbers indicate faster consumption. The sign is reversed for the corrected rate in the following column.	umol oxygen/hour	resp
rate_corr	corrected oxygen consumption rate of the limpet; corrected using the averaged rate of the blank chambers for that trial run. NOTE: the sign of the rate has been reversed here so that faster consumption rates are larger positive values instead of lower negative values.	umol oxygen/hour	resp
r_squared	the R-squared value of the straight-line regression fit to the oxygen consumption data used to derive the raw oxygen consumption rate given above. Provided as a goodness-of-fit metric of the regression used to calculate oxygen consumption rate.	unitless	resp
resid_var	the variance of the residuals around the straight-line regression fit of the oxygen consumption data versus time. Provided as a metric of the goodness-of-fit of the regression used to calculate oxygen consumption rate.	unitless	resp
rate_mass_specific_O2	oxygen consumption rate divided by the dry tissue mass of the limpet.	umol oxygen/hour/gram dry tissue.	O2_consum
date_trial	Date of the trial run. All limpets were collected between 2 and 7 days prior to the trial date on which they were used.	mm/dd/yyyy	date

Database

Contribute Data

Dataset: limpet aerial respiration
Deployment: Denny_2013

Dataset acquisition description

Dataset Processing Description

Database

Contribute Data

Dataset: limpet aerial respirationDeployment: Denny_2013

Dataset acquisition description

Dataset Processing Description

Dataset: limpet aerial respiration
Deployment: Denny_2013