Dataset: Trichodesmium consortia metagenomics
Deployment: lab_Webb_Hutchins_Fu

16S tag data, metagenomic data, and a draft assembled genome of Altermonas macleodii derived from laboratory-maintained Trichodesmium erythraeum cultures
Principal Investigator: 
David A. Hutchins (University of Southern California, USC)
Co-Principal Investigator: 
Eric A. Webb (University of Southern California, USC)
BCO-DMO Data Manager: 
Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)
Current State: 
Final with updates expected
Version: 
14 March 2017
Version Date: 
2017-03-14
Description

These data are all derived from laboratory-maintained Trichodesmium erythraeum cultures and contain 16S tag data, metagenomic data, and a draft assembled genome of Altermonas macleodii.

3 DNA sequence datasets have been uploaded to NCBI in their appropriate locations. The accession information is presented here:

FastQ files of 16S rRNA amplicon data have been uploaded to NCBI’s Sequence Read Archive (SRA) under accession number SRP078329.

FastQ files of 5 metagenomic sequence datasets have been deposited in NCBI’s SRA under SRP078343.

A FastA file of a draft genome has been uploaded to NCBI’s Whole Genome Shotgun (WGS) database under the accession MBSN00000000.

These datasets were released upon publication of the paper: The Trichodesmium consortium: conserved heterotrophic co-occurrence and genomic signatures of potential interactions, by Lee et al. (doi:10.1038/ismej.2017.49), published in April 2017. Detailed sample information is included in the supplemental spreadsheets to that publication, specifically tables S1 and S2. 

These data were also utilized in the paper:
Walworth, N. G., Lee, M. D., Suffridge, C., Qu, P., Fu, F., Saito, M. A., ... & Hutchins, D. A. (2018). Functional genomics and phylogenetic evidence suggest genus-wide cobalamin production by the globally distributed marine nitrogen fixer Trichodesmium. Frontiers in Microbiology9, 189. doi: 10.3389/fmicb.2018.00189

More information about this dataset deployment