Dataset: Fatty acid profiles
Deployment: AP_Rankin

Fatty acid profiles of M. menidia females and their unfertilized eggs.
Principal Investigator: 
Hannes Baumann (University of Connecticut, UConn)
Co-Principal Investigator: 
Janet Nye (Stony Brook University - SoMAS, SUNY-SB SoMAS)
Hannes Baumann (University of Connecticut, UConn)
BCO-DMO Data Manager: 
Hannah Ake (Woods Hole Oceanographic Institution, WHOI BCO-DMO)
Current State: 
Final no updates expected

Gas chromatography was used to quantify the absolute (mg g dry weight-1) and relative concentrations (% of total) of 27 FAs for each of 5 females (whole individual) and their unfertilized eggs (~ 1 ml). Samples were first dried and then homogenized in a solution of chloroform-methanol (2:1 v/v) and tricosanoic acid (23:0) as an internal standard for quantification of mg g−1 dry mass of fatty acids. Lipids were cold-extracted from approximately 50 mg of dry mass. A Shimadzu GC-2014 gas chromatograph set with a Phenomenex ZB-WAX plus capillary column (30 m long; 0.53 mm ID; 1.0 um thick) was used to quantify FAs, and individual FAs were identified by comparison to commercial standards (Supelco, Inc). 

These data are associated with the corresponding paper:

Snyder, J.T.*, Murray, C.S.*, and Baumann, H. (2017) Potential for maternal effects on offspring CO2-sensitivities in a coastal marine fish. Journal of Experimental Marine Biology and Ecology (in press).

Other datasets related to this paper:

Survival, length, and growth responses of M. menidia offspring from different females exposed to contrasting CO2 environments.

More information about this dataset deployment