Dataset: 16S rRNA gene from RNA
Deployment: Chikyu-331

16S rRNA gene (from RNA)
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Principal Investigator: 
Christopher House (Pennsylvania State University, PSU)
Leah Brandt (Pennsylvania State University, PSU)
BCO-DMO Data Manager: 
Shannon Rauch (Woods Hole Oceanographic Institution, WHOI BCO-DMO)
Deployment Synonyms:

Next-generation sequencing data across a hydrothermal gradient; Okinawa Backarc Basin IODP 331 – subvent biosphere. RNA extractions were attempted from sediments at Sites C0014 and C0017 to selectively investigate the active microbial populations at these sites. This study was conducted to supplement the gene-based and metagenomic studies. However, the results from sequenced reverse transcribe 16S rRNA demonstrate the sensitivity of extraction and amplification to external nucleic acids and attest to the challenges in working with low biomass environmental samples. Inconsistencies between primersets and replicates make conclusions about the results of this RNA study extremely tenuous.

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