Instrument: Turner Designs Fluorometer -10-AU
The Turner Designs 10-AU Field Fluorometer is used to measure Chlorophyll fluorescence. The 10AU Fluorometer can be set up for continuous-flow monitoring or discrete sample analyses. A variety of compounds can be measured using application-specific optical filters available from the manufacturer. (read more from Turner Designs, turnerdesigns.com, Sunnyvale, CA, USA)
Seawater samples collected from Niskin bottles were filtered over 25 mm Whatman GF/F filters under low vacuum pressure, extracted overnight at 4C in 5mL 90% acetone and analyzed on a Turner Model 10AU fluorometer before and after acidification. The fluorometer was calibrated with pure chlorophyll a from Sigma.