Instrument: High Performance Liquid Chromatograph

Instrument Short Name: HPLC
Instrument Description:

A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase.

PI supplied instrument name: High Performance Liquid Chromatograph
Dataset-specific description

HPLC analysis was performed using a Shimadzu HPLC equipped with a monomeric (Rainin Microsorb-MV, 0.46 x 10 cm, 3 µm) and a polymeric (Vydac 201TP54, 0.46 x 25 cm, 5 um) reverse-phase C18 column in series. Absorption spectra and chromatograms (440 ± 4 nm) were acquired using a Shimadzu SPD-M10av photodiode array detector.