Instrument: High Performance Liquid Chromatograph

Instrument Short Name: HPLC
(http://vocab.nerc.ac.uk/collection/L05/current/LAB11/)
Instrument Description:

A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase.

PI supplied instrument name: HPLC
Dataset-specific description

HPLC analysis was restarted in July 2006 (CAR-123). Samples were filtered 47 mm Whatman GF/F filters at 8 depths (1, 7, 15, 25, 35, 55, 75 and 100m). The volume filtered depends on the amount of particles in the water. Replicates were taken at the 1m depth. Filters were stored in aluminum envelopes and stored in the fridge until reaching shore. Once on shore, samples were stored at -40°C until transportation to the US. Horn Point Laboratory (http://www.hpl.umces.edu/) performs the analyses through a collaborative agreement with NASA. The method used was Van Heukelem and Thomas (2001).