Dataset: Dissolved nitrite and ammonium concentration data from R/V Atlantis (AT15-61) cruise in Jan-Feb 2010 and R/V Melville (MV1104) cruise in Mar-Apr 2011 in the Eastern Tropical South Pacific

ValidatedFinal no updates expectedDOI: 10.26008/1912/bco-dmo.820165.1Version 1 (2020-08-06)Dataset Type:Cruise Results

Lead Principal Investigator: Karen L. Casciotti (Woods Hole Oceanographic Institution)

Scientist: Angela N. Knapp (Florida State University EOAS)

Contact: Alyson E. Santoro (University of California-Santa Barbara)

BCO-DMO Data Manager: Dana Stuart Gerlach (Woods Hole Oceanographic Institution)


Project: Expression of Microbial Nitrification in the Stable Isotopic Systematics of Oceanic Nitrite and Nitrate (Microbial Nitrification)


Abstract

Dissolved nitrite ([NO2-]) and ammonium ([NH4+]) concentration data were collected on two cruises in the Eastern Tropical South Pacific. Data from R/V Atlantis (AT15-61) cruise collected in Jan-Feb 2010 and data from R/V Melville (MV1104) cruise collected in Mar-Apr 2011 as part of the Microbial Nitrification project.

Seawater samples were obtained during the R/V Atlantis (AT15-61) and R/V Melville (MV1104) cruises in Jan-Feb 2010 and Mar-Apr 2011.  Water samples were collected at discrete depths using a standard 24-bottle Niskin rosette sampler equipped with an SBE9plus conductivity-temperature-depth (CTD) sensor package (SeaBird Electronics, Bellevue, WA). 

Ammonium concentration ([NH4+]) was determined on-ship in unfiltered 50 mL seawater samples using o-phthaldialdehyde derivatization (Holmes et al., 1999) and measurement on an Aquafluor 8000 handheld fluorometer (Turner Designs), with modifications as suggested in Taylor et al., 2007. Nitrite ([NO2-]) was determined on-ship in unfiltered 50 mL sample volumes using standard colorimetric methods (Strickland and Parsons, 1968).  NH4+ standards (30 – 300 nM) were freshly prepared for each analysis in duplicate using deep water (> 500 m) from the same station, which consistently had a lower blank than ultrapure water. Detection limits for [NH4+] and  [NO2-] were 0.010 µM, and 0.10 µM for [NO2-]. Measured values are reported even if they are lower than the detection limit.


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Results

Santoro, A. E., Buchwald, C., Knapp, A. N., Berelson, W. M., Capone, D. G., & Casciotti, K. L. (2020). Nitrification and nitrous oxide production in the offshore waters of the Eastern Tropical South Pacific. doi:10.1002/essoar.10503499.1
Methods

Holmes, R. M., Aminot, A., Kérouel, R., Hooker, B. A., & Peterson, B. J. (1999). A simple and precise method for measuring ammonium in marine and freshwater ecosystems. Canadian Journal of Fisheries and Aquatic Sciences, 56(10), 1801–1808. doi:10.1139/f99-128
Methods

Strickland, J.D.H and Parsons, T.R. (1968) A Practical Handbook of Seawater Analysis. Fisheries Research Board of Canada Bulletin 167, 71-75 [as seen in The Quarterly Review of Biology (1969) 44(3), 327–327. doi:10.1086/406210]
Methods

Taylor, B. W., Keep, C. F., Hall, R. O., Koch, B. J., Tronstad, L. M., Flecker, A. S., & Ulseth, A. J. (2007). Improving the fluorometric ammonium method: matrix effects, background fluorescence, and standard additions. Journal of the North American Benthological Society, 26(2), 167–177.