http://lod.bco-dmo.org/id/dataset/1000873 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2026-06-11 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Sediment methane and sulfate concentrations and radiotracer anaerobic methane oxidation and sulfate reduction rates in the Gulf of California during R/V Atlantis cruise AT50-22 in 2024 2026-09-04 publication 2026-09-04 revision BCO-DMO Linked Data URI 2026-09-04 creation http://lod.bco-dmo.org/id/dataset/1000873 Samantha B. Joye University of Georgia principalInvestigator Kimberley Hunter University of Georgia principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Joye, S. B., Hunter, K. (2026) Sediment methane and sulfate concentrations and radiotracer anaerobic methane oxidation and sulfate reduction rates in the Gulf of California during R/V Atlantis cruise AT50-22 in 2024. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-09-04 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/1000873 [access date] AT50-22 sediment rates Dataset Description: Methods and Sampling: &lt;p&gt;Sediment samples were collected by the HOV Alvin using PVC push cores. Upon arrival at the surface, the cores were described and cataloged prior to being sectioned into discrete 3 cm depth intervals. Methane samples were collected first. &amp;nbsp;Porewater was separated from the sediment using a manually-actuated porewater press as described by Joye et al., 2004. Whole sediment subsamples for radiotracer sulfate reduction rates and anaerobic oxidation of methane rates were collected in triplicate from a parallel core and were handled, injected, incubated, and analyzed using the methods described in Joye et al., 2004.&lt;/p&gt; &lt;p&gt;CH4: Methane samples (3 cc whole sediment) were collected into a glass serum vial, preserved with 2 mL 2 M N₂-purged NaOH, crimp-sealed with a butyl rubber stopper and stored at room temperature until analysis. Concentrations were determined by headspace analysis using an SRI 8610 GC-FID with Agilent J&amp;amp;W HP-PLOT Al2O3 S Capillary Column, 50 m, 0.53 mm, 15.00 μm (Prod. No. 19095P-S25E). See Joye et al., 2004.&lt;/p&gt; &lt;p&gt;SO4: Water sample was filtered through a pre-rinsed 0.2 μm regenerated cellulose Target2 syringe filter (Thermo Scientific, Prod. No. F25047) into a 7 mL scintillation vial. Samples were acidified with 10 μL of concentrated HNO3 per 1 mL sample, sealed with a PTFE-lined cap, and stored at room temperature until analysis. Sample analysis was performed with a Dionex Integrion HPIC using KOH eluent supplied by a Dionex EGC 500 KOH Eluent Generator Cartridge (Prod. No. 075778), Dionex CR-ATC Continuously Regenerated Trap Column (Prod. No. 088662), Dionex ADRS 600 Dynamically Regenerated Suppressor (Prod. No. 088666), Dionex IonPac AG18 Guard Column (Prod. No. 060551), Dionex IonPac AS18 Analytical Column (Prod. No. 060549) and Dionex CRD 200 Carbonate Removal Device (Prod. No. 062983). See Weston et al., 2006.&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2049439 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2049439 onGoing Samantha B. Joye University of Georgia 706-542-5893 Dept. of Marine Sciences 325 Sanford Drive Athens GA 30602 USA mjoye@uga.edu pointOfContact Kimberley Hunter University of Georgia kshunter@uga.edu pointOfContact asNeeded Dataset Version: 1 Unknown Site Collection_DateTime Collection_Date Collection_Time Alvin_Dive_number Lat Long Depth Depth_Range Midpoint_Depth CH4 SO4 SO4_flag Anaerobic_Methane_Oxidation Sulfate_Reduction SRI 8610 GC-FID HOV Alvin VWR refrigerated incubator model 89508-420 Dionex Integrion HPIC with Dionex AS-AP Autosampler Perkin Elmer Tri-Carb 2910TR liquid scintillation counter PVC push cores manually-actuated porewater press theme None, User defined site ISO_DateTime_UTC date time of day dive_id latitude longitude depth depth below seafloor sample description methane sulfate quality flag oxidation sulfate_reduction featureType BCO-DMO Standard Parameters Gas Chromatograph HOV Alvin Incubator Ion Chromatograph Liquid Scintillation Counter Push Corer Sediment Porewater Sampler instrument BCO-DMO Standard Instruments AT50-22 service Deployment Activity Gulf of California place Locations otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Collaborative research: Regulation and dynamics of microbial communities and biogeochemical cycling in hydrothermally-influenced habitats in the Gulf of California https://osprey.bco-dmo.org/project/876801 Collaborative research: Regulation and dynamics of microbial communities and biogeochemical cycling in hydrothermally-influenced habitats in the Gulf of California <p><em>NSF Award Abstract:</em><br /> Oceanic hydrothermal ecosystems have captivated the imagination of scientists and the general public since their discovery ~40 years ago. These habitats are characterized by extremes in temperature and pH, low oxygen concentrations, and high concentrations of toxic metals. Despite this, these ecosystems support rich and abundant microbial communities that achieve high rates of biogeochemical cycling. This project supports unprecedented studies to identify the impact of chemical regimes on microbial and viral community composition, diversity, and activity in areas in the Gulf of California along a range of hydrothermalism and dissolve oxygen levels. The project provides training opportunities for undergraduate and graduate students. Results are communicated through talks and lectures, publications, and data sharing through public repositories. The work will be shared through Ocean Discovery Camp and Clubs for diverse middle school students, displays at the Georgia Museum of Art, and a collaboration with the BBC Planet Earth III – Oceans team. Through these cumulative efforts, the project will forge a strong legacy in education and in fostering ocean literacy and promoting ocean advocacy in the general public.</p> <p>The Gulf of California is a system where hydrothermal fluids flow through and alter sediment prior to discharge into deep waters. In sediments, fluid flow modulates biological dynamics through changes in carbon loading and electron accepter availability. In the water column, inorganic and organic energy sources are injected into hypoxic deep waters, creating dynamic chemical niches. This project studies how gradients in geochemistry shape and modulate the microbial and viral communities that carry out key biogeochemical reactions in sediments and in the water column of Guaymas and Pescadero Basins. The research integrates data streams from biogeochemistry, genomics, and microbiology, including single-cell activity approaches, to achieve unprecedented insight into regulatory mechanisms and dynamics. The project includes experiments and observations in the laboratory and at sea during an expedition on the R/V Atlantis with the deep submergence vehicle ALVIN in 2022. Key topics for investigation include: (1) Do variations in geochemical regimes select for metabolically plastic microbial populations? (2) Do different microbes become active under specific geochemical conditions or do the same microbes adapt to changing geochemical conditions? (3) What is the role of viruses in shaping the microbial populations present in highly dynamic hydrothermal habitats?</p> <p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p> GoC Microbial Biogeochem largerWorkCitation project eng; USA oceans Gulf of California -111.46374 -108.85566 23.94152 27.58194 2024-04-08 2024-04-25 Gulf of California 0 BCO-DMO catalogue of parameters from Sediment methane and sulfate concentrations and radiotracer anaerobic methane oxidation and sulfate reduction rates in the Gulf of California during R/V Atlantis cruise AT50-22 in 2024 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/1007011.rdf Name: Site Units: unitless Description: <p>Site name</p> http://lod.bco-dmo.org/id/dataset-parameter/1007012.rdf Name: Collection_DateTime Units: unitless Description: <p>Datetime of sample collection</p> http://lod.bco-dmo.org/id/dataset-parameter/1007014.rdf Name: Collection_Date Units: unitless Description: <p>Date of sample collection</p> http://lod.bco-dmo.org/id/dataset-parameter/1007015.rdf Name: Collection_Time Units: unitless Description: <p>Time of sample collection (24 hr)</p> http://lod.bco-dmo.org/id/dataset-parameter/1007016.rdf Name: Alvin_Dive_number Units: unitless Description: <p>Alvin dive number</p> http://lod.bco-dmo.org/id/dataset-parameter/1007017.rdf Name: Lat Units: decimal degrees Description: <p>Latitude of sample collection, positive is North</p> http://lod.bco-dmo.org/id/dataset-parameter/1007019.rdf Name: Long Units: decimal degrees Description: <p>Longitude of sample collection, negative is West</p> http://lod.bco-dmo.org/id/dataset-parameter/1007021.rdf Name: Depth Units: meters (m) Description: <p>Seafloor depth from which sample was collected</p> http://lod.bco-dmo.org/id/dataset-parameter/1007023.rdf Name: Depth_Range Units: centimeters (cm) Description: <p>The depth interval below the seafloor from which the sample was taken. OLW = Overlying water sample.</p> http://lod.bco-dmo.org/id/dataset-parameter/1007024.rdf Name: Midpoint_Depth Units: centimeters (cm) Description: <p>The mid-point depth of the sediment layer from which the sample was taken. OLW = Overlying water sample.</p> http://lod.bco-dmo.org/id/dataset-parameter/1007025.rdf Name: CH4 Units: micromolar (µM) Description: <p>Methane. Method detection limit = 1 µM</p> http://lod.bco-dmo.org/id/dataset-parameter/1007026.rdf Name: SO4 Units: millimolar (mM) Description: <p>Sulfate. Method detection limit = 0.1 mM. See SO4_flag for Below detection limit indicator</p> http://lod.bco-dmo.org/id/dataset-parameter/1007027.rdf Name: SO4_flag Units: unitless Description: <p>Sulfate indicator: BDL = Below detection limit. Method detection limit = 0.1 mM</p> http://lod.bco-dmo.org/id/dataset-parameter/1007028.rdf Name: Anaerobic_Methane_Oxidation Units: nmol cm⁻³ d⁻¹ Description: <p>Anaerobic oxidation of methane rate</p> http://lod.bco-dmo.org/id/dataset-parameter/1007029.rdf Name: Sulfate_Reduction Units: nmol cm⁻³ d⁻¹ Description: <p>Sulfate reduction rate</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact https://osprey.bco-dmo.org/dataset/1000873/data/download download onLine dataset &lt;p&gt;Sediment samples were collected by the HOV Alvin using PVC push cores. Upon arrival at the surface, the cores were described and cataloged prior to being sectioned into discrete 3 cm depth intervals. Methane samples were collected first. &amp;nbsp;Porewater was separated from the sediment using a manually-actuated porewater press as described by Joye et al., 2004. Whole sediment subsamples for radiotracer sulfate reduction rates and anaerobic oxidation of methane rates were collected in triplicate from a parallel core and were handled, injected, incubated, and analyzed using the methods described in Joye et al., 2004.&lt;/p&gt; &lt;p&gt;CH4: Methane samples (3 cc whole sediment) were collected into a glass serum vial, preserved with 2 mL 2 M N₂-purged NaOH, crimp-sealed with a butyl rubber stopper and stored at room temperature until analysis. Concentrations were determined by headspace analysis using an SRI 8610 GC-FID with Agilent J&amp;amp;W HP-PLOT Al2O3 S Capillary Column, 50 m, 0.53 mm, 15.00 μm (Prod. No. 19095P-S25E). See Joye et al., 2004.&lt;/p&gt; &lt;p&gt;SO4: Water sample was filtered through a pre-rinsed 0.2 μm regenerated cellulose Target2 syringe filter (Thermo Scientific, Prod. No. F25047) into a 7 mL scintillation vial. Samples were acidified with 10 μL of concentrated HNO3 per 1 mL sample, sealed with a PTFE-lined cap, and stored at room temperature until analysis. Sample analysis was performed with a Dionex Integrion HPIC using KOH eluent supplied by a Dionex EGC 500 KOH Eluent Generator Cartridge (Prod. No. 075778), Dionex CR-ATC Continuously Regenerated Trap Column (Prod. No. 088662), Dionex ADRS 600 Dynamically Regenerated Suppressor (Prod. No. 088666), Dionex IonPac AG18 Guard Column (Prod. No. 060551), Dionex IonPac AS18 Analytical Column (Prod. No. 060549) and Dionex CRD 200 Carbonate Removal Device (Prod. No. 062983). See Weston et al., 2006.&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;CH4 data was processed using SRI Instruments PeakSimple software. &amp;nbsp;SO4 data was processed using DIONEX Chromeleon software. &amp;nbsp;Radioisotope data was processed using PerkinElmer QuantaSmart software.&lt;/p&gt; Specified by the Principal Investigator(s) This section documents curation actions performed prior to publication review with the submitter, and additional information relevant to understanding and reusing this dataset. It distinguishes changes made to the submitted (meta)data from unresolved issues and/or enhancements that improve future reuse and interoperability. CURATION ACTIONS PERFORMED ON DATA - Loaded AT50-22 Sediment Rates V2.xlsx (Sheet1), header row 8, units row 9 skipped, blank trailing columns dropped, missing values &quot;&quot;, &quot;nd&quot;, &quot;ND&quot; - Renamed columns to BCO-DMO convention: Collection Date to Collection_Date, Collection Time to Collection_Time, Alvin Dive # to Alvin_Dive_number, Lat (N) to Lat, Long (W) to Long, Depth Range to Depth_Range, Mid-point Depth to Midpoint_Depth, Sulfate Reduction to Sulfate_Reduction, Anaerobic Methane Oxidation to Anaerobic_Methane_Oxidation - Normalized Collection_Date values: slashes converted to hyphens, single-digit day zero-padded, single-digit month zero-padded, yielding consistent dd-mm-yy hyphenated format - Converted Collection_Date from dd-mm-yy string to ISO date (%Y-%m-%d) - Extracted non-numeric values from SO4 column into new SO4_flag column (suffix _flag), preserving metadata, isolating &quot;BDL&quot; (below detection limit) entries from numeric sulfate values - Combined Collection_Date and Collection_Time into new datetime column Collection_DateTime, ISO 8601 formatted - Reordered columns: Site, Collection_DateTime, Collection_Date, Collection_Time, Alvin_Dive_number, Lat, Long, Depth, Depth_Range, Midpoint_Depth, CH4, SO4, SO4_flag, Anaerobic_Methane_Oxidation, Sulfate_Reduction - Output as 1000873_v1_at50_22_sediment_rates.csv CURATION ACTIONS PERFORMED ON METADATA - BCO-DMO's standard metadata entry and text formatting steps were performed. - Used the provided information to add context to the abstract. ISSUES POTENTIALLY IMPACTING REUSE - Collection times provided do not clarify timezone. Specified by BCO-DMO Data Managers asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact SRI 8610 GC-FID SRI 8610 GC-FID PI Supplied Instrument Name: SRI 8610 GC-FID PI Supplied Instrument Description:Concentrations were determined by headspace analysis using an SRI 8610 GC-FID with Agilent J&W HP-PLOT Al2O3 S Capillary Column, 50 m, 0.53 mm, 15.00 μm (Prod. No. 19095P-S25E). Instrument Name: Gas Chromatograph Instrument Short Name:Gas Chromatograph Instrument Description: Instrument separating gases, volatile substances, or substances dissolved in a volatile solvent by transporting an inert gas through a column packed with a sorbent to a detector for assay. (from SeaDataNet, BODC) Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB02/ HOV Alvin HOV Alvin PI Supplied Instrument Name: HOV Alvin PI Supplied Instrument Description:Sediment samples were collected by the HOV Alvin using PVC push cores.  Instrument Name: HOV Alvin Instrument Short Name: Instrument Description: Human Occupied Vehicle (HOV) Alvin is part of the National Deep Submergence Facility (NDSF). Alvin enables in-situ data collection and observation by two scientists to depths reaching 6,500 meters, during dives lasting up to ten hours. Commissioned in 1964 as one of the world’s first deep-ocean submersibles, Alvin has remained state-of-the-art as a result of numerous overhauls and upgrades made over its lifetime. The most recent upgrades, begun in 2011 and completed in 2021, saw the installation of a new, larger personnel sphere with a more ergonomic interior; improved visibility and overlapping fields of view; longer bottoms times; new lighting and high-definition imaging systems; improved sensors, data acquisition and download speed.  It also doubled the science basket payload, and improved the command-and-control system allowing greater speed, range and maneuverability. With seven reversible thrusters, it can hover in the water, maneuver over rugged topography, or rest on the sea floor.  It can collect data throughout the water column, produce a variety of maps and perform photographic surveys.  Alvin also has two robotic arms that can manipulate instruments, obtain samples, and its basket can be reconfigured daily based on the needs of the upcoming dive. Alvin's depth rating of 6,500m gives researchers in-person access to 99% of the ocean floor.  Alvin is a proven and reliable platform capable of diving for up to 30 days in a row before requiring a single scheduled maintenance day.  Recent collaborations with autonomous vehicles such as Sentry have proven extremely beneficial, allowing PIs to visit promising sites to collect samples and data in person within hours of their being discovered, and UNOLs driven technological advances have improved the ability for scientific outreach and collaboration via telepresence Alvin is named for Allyn Vine, a WHOI engineer and geophysicist who helped pioneer deep submergence research and technology. (from https://www.whoi.edu/what-we-do/explore/underwater-vehicles/hov-alvin/, accessed 2022-09-09) VWR refrigerated incubator model 89508-420 VWR refrigerated incubator model 89508-420 PI Supplied Instrument Name: VWR refrigerated incubator model 89508-420 PI Supplied Instrument Description:Whole sediment subsamples for radiotracer sulfate reduction rates and anaerobic oxidation of methane rates were collected in triplicate from a parallel core and were handled, injected, incubated, and analyzed using the methods described in Joye et al., 2004. Instrument Name: Incubator Instrument Short Name:incubator (general) Instrument Description: A device in which environmental conditions (light, photoperiod, temperature, humidity, etc.) can be controlled. Note: we have more specific terms for shipboard incubators (https://www.bco-dmo.org/instrument/629001) and in-situ incubators (https://www.bco-dmo.org/instrument/494). Dionex Integrion HPIC with Dionex AS-AP Autosampler Dionex Integrion HPIC with Dionex AS-AP Autosampler PI Supplied Instrument Name: Dionex Integrion HPIC with Dionex AS-AP Autosampler PI Supplied Instrument Description:Sample analysis was performed with a Dionex Integrion HPIC using KOH eluent supplied by a Dionex EGC 500 KOH Eluent Generator Cartridge (Prod. No. 075778), Dionex CR-ATC Continuously Regenerated Trap Column (Prod. No. 088662), Dionex ADRS 600 Dynamically Regenerated Suppressor (Prod. No. 088666), Dionex IonPac AG18 Guard Column (Prod. No. 060551), Dionex IonPac AS18 Analytical Column (Prod. No. 060549) and Dionex CRD 200 Carbonate Removal Device (Prod. No. 062983). Instrument Name: Ion Chromatograph Instrument Short Name:Ion Chromatograph Instrument Description: Ion chromatography is a form of liquid chromatography that measures concentrations of ionic species by separating them based on their interaction with a resin. Ionic species separate differently depending on species type and size. Ion chromatographs are able to measure concentrations of major anions, such as fluoride, chloride, nitrate, nitrite, and sulfate, as well as major cations such as lithium, sodium, ammonium, potassium, calcium, and magnesium in the parts-per-billion (ppb) range. From: http://serc.carleton.edu/microbelife/research_methods/biogeochemical/ic.html Perkin Elmer Tri-Carb 2910TR liquid scintillation counter Perkin Elmer Tri-Carb 2910TR liquid scintillation counter PI Supplied Instrument Name: Perkin Elmer Tri-Carb 2910TR liquid scintillation counter PI Supplied Instrument Description:Perkin Elmer Tri-Carb 2910TR liquid scintillation counter Instrument Name: Liquid Scintillation Counter Instrument Short Name:LSC Instrument Description: Liquid scintillation counting is an analytical technique which is defined by the incorporation of the radiolabeled analyte into uniform distribution with a liquid chemical medium capable of converting the kinetic energy of nuclear emissions into light energy. Although the liquid scintillation counter is a sophisticated laboratory counting system used to quantify the activity of particulate emitting (ß and a) radioactive samples, it can also detect the auger electrons emitted from 51Cr and 125I samples. Liquid scintillation counters are instruments assaying alpha and beta radiation by quantitative detection of visible light produced by the passage of rays or particles through a suitable scintillant incorporated into the sample. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB21/ PVC push cores PVC push cores PI Supplied Instrument Name: PVC push cores PI Supplied Instrument Description:Sediment samples were collected by the HOV Alvin using PVC push cores.  Instrument Name: Push Corer Instrument Short Name: Instrument Description: Capable of being performed in numerous environments, push coring is just as it sounds. Push coring is simply pushing the core barrel (often an aluminum or polycarbonate tube) into the sediment by hand. A push core is useful in that it causes very little disturbance to the more delicate upper layers of a sub-aqueous sediment. Description obtained from: http://web.whoi.edu/coastal-group/about/how-we-work/field-methods/coring/ manually-actuated porewater press manually-actuated porewater press PI Supplied Instrument Name: manually-actuated porewater press PI Supplied Instrument Description:Porewater was separated from the sediment using a manually-actuated porewater press as described by Joye et al. 2004.  Instrument Name: Sediment Porewater Sampler Instrument Short Name: Instrument Description: A device that collects samples of pore water from various horizons below the seabed. Cruise: AT50-22 AT50-22 R/V Atlantis Community Standard Description International Council for the Exploration of the Sea R/V Atlantis vessel AT50-22 Samantha B. Joye University of Georgia R/V Atlantis Community Standard Description International Council for the Exploration of the Sea R/V Atlantis vessel