14:I[71133,["4507","static/chunks/e2799680-446c489506865058.js","7665","static/chunks/7665-81c5407bc4489ff9.js","8500","static/chunks/8500-e4dd1cb1bb626827.js","9753","static/chunks/9753-028c0a26aa71f2d4.js","8437","static/chunks/8437-a09d0b83fcec76af.js","4557","static/chunks/4557-64c5efa221bc528f.js","947","static/chunks/947-410627998426400f.js","5357","static/chunks/5357-6142894e88cb7da2.js","8382","static/chunks/app/dataset/%5Bid%5D/data/view/page-177ea8adea7aed86.js"],"default"] 15:T1902,

These are data on kelp traits, seawater features and 16S rRNA amplicon sequencing. All kelp measurements were made in situ, often via kayak or at the shore using a measuring tape and calipers.

Kelp tissue was collected for elemental analysis by excising a 2 x 2 centimeter (cm) piece of tissue in the meristematic region. Tissue was ground to a fine powder in a GenoGrinder Spex (Metuchen, NJ), weighed in aliquots of 1.2 to 1.5 milligrams (mg) and packed into 3.5 × 5 millimeter (mm) aluminium tins (Costech, Valencia, CA). Packed tins were analysed on an elemental analyser isotope ratio mass spectrometer at Northwestern University Stable Isotope Biogeochemistry Laboratory (NUSIBL).

Carbon fixation was quantified using a section of the youngest, basal frond tissue extending 24 to 30 cm from the bulb. It was weighed (wet mass in grams (g)) with a Pesola scale and placed in a 1 liter (L) Nalgene container; the change in oxygen was measured for 1 hour with a Pyro Science contactless fibre-optic sensors (Oxygen Sensor Spots OXSP5) and an optical oxygen and temperature meter (FireStingO2 FSO2-4), with the sensor spots affixed inside each 1 L chamber.

DOC release and nutrient changes were quantified from seawater filtered through a GF/F filter and immediately frozen for analysis at the University of Washington Marine Chemistry Laboratory (methods from UNESCO), with DOC samples frozen in 40 milliliter (mL) glass vials with Teflon caps (Shimadzu, VOA) and other nutrients in acid-washed 60 mL high density polyethylene (HDPE) Nalgene bottles.

Using pulse amplitude modulated (PAM) fluorometry in situ (Diving Pam, Walz, Germany) we estimated fluorescence over a range of irradiances. PAM is a non-invasive, in situ method that quantifies Photosystem II fluorescence parameters across different light levels. Tissue was dark-adapted for 20 minutes and then rapid light curves were estimated with nine PAR levels from 0 to 1200. We estimated the maximum electron transport rate (ETR_max) and the photosynthetic efficiency at low light, both as the initial linear slope of ETR to irradiance (α) and as the quantum yield of photosystem II (Yield) on nine individual Nereocystis at both Tatoosh Island and Squaxin Island, the populations that span the greatest range of environmental conditions. Tatoosh Island was assayed on 9 and 20 June 2019 and Squaxin Island on 2 July 2019. At both sites, we selected individuals at least 1 meter (m) apart at low tide.

The salinity and seawater temperatures were assessed with a Castaway-CTD (Sontek) from a boat or kayak at all sites except Tatoosh Island where instrumentation is moored (Hach DS5).

Microbial density on individual kelp blades at either end of the geographic distribution, Tatoosh Island versus Squaxin Island, differed using 4,6-diamidino-2-phenylindole (DAPI) fluorescent staining of bacterial cells. We quantified bacterial cells from a section of the blade at approximately 20 cm distal from where the blade articulates with the bulb. We sampled a 3−5 mm slice from 10 individuals at each site (Tatoosh on 4 August 2019, Squaxin on 2 July 2019), preserving each slice immediately in Formalin, transferring to 50 : 50 EtOH : phosphate-buffered saline (PBS) after 1 hour, then freezing. In the laboratory, each piece was again sliced with a razor blade to approximately 0.5 mm, placed on a glass slide, and a solution of 1 microgram (µg) DAPI : 1 mL PBS was dropped on the slide-mounted slice. The slide was kept in dark for 20 minutes, chilled and rinsed with PBS prior to visualizing at 100× with an Olympus BX50 fluorescence microscope with a DAPI filter. We photographed 10 fields of view, selected haphazardly, for each individual slice, resulting in 100 images at each Tatoosh and Squaxin. In samples where bacteria were sparse or non-existent, we repeatedly adjusted the fine focus to verify no bacteria were present. We quantified the bacteria in ImageJ, enumerating the cells in the blue channel, adjusting the background and thresholds identically for all 200 images to eliminate autofluorescence by kelp cells. We calculated bacterial cell density as a percentage of the total imaged area.

Individual kelp was sampled for microbes by rubbing a cotton swab for 20−30 seconds over the mid-blade region. The swab was placed in a 2 mL Eppendorf tube, immediately chilled and transferred to a freezer within 4−6 hours. DNA from the swabs was extracted with a Qiagen DNeasy PowerSoil Kit (Qiagen). DNA was amplified, sequenced and amplicon sequence variants (ASVs) were identified by the Duchossois Family Institute Microbial Metagenomics Facility (DFIMMF) at The University of Chicago. The V4–V5 region within the 16S ribosomal RNA (rRNA) gene was amplified using universal bacterial primers and polymerase chain reaction (PCR) conditions described in Younker, et al. (2024). Approximately 412 bp region amplicons were then purified using a spin column-based method (Minelute, Qiagen), quantified and dual index adapters were ligated. Sequences were generated from the Illumina MiSeq platform using the QIASeq 1-step amplicon kit (Qiagen) for generating libraries and using 2 × 250 paired end reads with 5000 to 10,000 reads per sample. The default pipeline for processing MiSeq 16S rRNA reads was dada2 (v. 1.18.0) with minor modifications in R (v. 4.0.3). Reads were first trimmed at 190 bp for both forward and reverse reads to remove low-quality nucleotides, and chimaeras were detected and removed using the default consensus method in the dada2 pipeline. Then, ASVs with length between 320 and 365 bp were retained. Taxonomy of the resultant ASVs were assigned to the genus level using the RDP classifier (v. 2.13) with a minimum bootstrap confidence score of 80. Species-level classification used blastn (v. 2.13.0) and the refseq_rna database. We analysed ASVs with R, phyloseq and microViz (R version 2023.06.2+561). Chloroplast DNA that was not classified as Cyanobacteria were generally diatoms (Bacillarophyta) and were filtered out, as well as any other chloroplast sequence that did not match to the class Cyanobacteria. Sequences of corn (Zea, in Plantae), in low numbers at some Puget Sound sites, were also removed.

All methods are described in the Pfister, et al. (2025) paper.

16:T724,Canopy kelp are foundational species in coastal ecosystems and host diverse bacterial communities. We used these data to test the association between bull kelp (Nereocystis luetkeana) host traits, blade-associated bacterial taxa and seawater environmental features across nine sites spanning more than 200 kilometers in Washington state. The data collection spans the outer coast site Tatoosh Island, at the entrance of the Strait of Juan de Fuca. Data collection extended eastward to include sites within the Strait of Juan de Fuca and central and southern Puget Sound. Kelp traits and seawater features that were measured are contained within this dataset and include carbon fixation and DOC release, morphometrics of kelp, and nutrient content. Seawater features that were measured were nutrients, sea surface temperature, and salinity in the surrounding water column. Microbial community data based on 16S rRNA amplicon sequencing of kelp blades across all sites are archived at the National Center for Biotechnology Information (NCBI) under BioProject number PRJNA1301461. Bacterial taxa showed differentiation among sites, and blade-associated bacterial densities were higher at the outer coast site compared with the most inland site. Yet, 11 bacterial genera were present in at least 80% of the samples; these taxa probably serve as core members of the N. luetkeana microbiome and show both positive and negative correlations with host health and environmental features. The data indicated strong interrelationships between kelp traits, seawater features and bacterial community composition with implications for the health of this highly productive foundational species in coastal ecosystems. This dataset supports the 2025 Pfister, et al. paper published in Royal Society Open Science (DOI: 10.1098/rsos.250637). 17:Tb66,NSF Award Abstract: It is increasingly recognized that species in the ocean host a diverse set of microbes and that these microbes can determine the functioning of the host. Yet, the discovery of microbial taxa in nature far outpaces our understanding of their functions. Primary producers in the ocean show rich microbial communities and may contribute to the significant role that macrophytes play in coastal communities. This project focuses on a species of canopy kelp that is a foundational species along the shores of the northeast Pacific Ocean, the bull kelp Nereocystis luetkeana. The research explores the role of a diverse set of microbes in association with bull kelp. Using experimental manipulations of newly isolated microbial taxa from kelp, tests of whether interactions are positive, negative, or neutral are performed. Specific assays of whether microbes provision vitamins and enhance access to nutrients are carried out, as well as tests of whether kelp exudates benefit microbial metabolism and growth. Working directly with Tribal youth through an internship program, as well as with undergraduate, post-baccalaureate, and graduate students, helps train the next generation of ocean scientists. Communication channels with Tribal and State governments inform efforts to understand kelp declines. Host-microbe interactions are a component of the persistence of ocean species and this research informs the factors that underly these relationships. The advent of DNA sequencing, imaging, and other molecular approaches have revealed that many key species in the ocean are a 'holobiome', and their fate is entwined with the microbes they host. Yet, this discovery of microbial taxa in nature far outpaces our understanding of microbial function. Nereocystis luetkeana, or bull kelp, and the bacteria the investigators have isolated from the kelp surface, present an opportunity to quantify host-microbe interactions, including how stressors of host health, such as ocean warming, alter these interactions. Experiments tracing stable nitrogen isotopes and manipulations with hosts and bacterial isolates are used to investigate possible exchanges and the currencies underlying interactions. Metabolomic, proteomic, and genomic analyses are used to quantify host-microbe interactions and linkage between taxa and their functions. Key microbial metabolisms being investigated include nutrient provisioning, vitamin synthesis, and the response to reactive oxygen species, among others. The demonstrated alteration of host-microbe interactions in the ocean when hosts are stressed suggest the findings of this study have application to understanding the factors that promote persistence of eukaryote-prokaryote partnerships. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.6:["$","$L14",null,{"dataset":{"_id":"1000920","description":"","problem":"","acquisition":"$15","processing":"

Basic statistical analysis was done with R, version 2023.06.2+561.

Packages used within R included phyloseq, microviz, ggplot, vegan, nlme, tidyverse

\n","bcodmoProcessing":"- Loaded data from original file \"Pfister_NereocystisGeography_RoySocOpen2025_BCODMO.csv\" into the BCO-DMO system, treating \"NA\" as a missing data value (missing data values are empty/blank in the final CSV file).\r\n- Converted date column from %m/%d/%y format to %Y-%m-%d format.\r\n- Saved the final file as \"1000920_v1_nereocystis_geography.csv\".","title":"Nereocystis luetkeana geographic patterns in physiology and microbiome from samples collected from 2019 to 2022 at nine sites spanning more than 200 kilometers in Washington state","nickname":"Nereocystis geographic patterns in physiology and microbiome","dataUrl":"","currentState":"Final no updates expected","currentStateId":"7","validated":true,"restricted":false,"version":"1","version_date":"2026-06-17T04:00:00.000Z","abstract":"$16","citation":"Pfister, C. A. (2026) Nereocystis luetkeana geographic patterns in physiology and microbiome from samples collected from 2019 to 2022 at nine sites spanning more than 200 kilometers in Washington state. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-06-17 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.1000920.1 [access date]","citationParts":{"identifier":"doi:10.26008/1912/bco-dmo.1000920.1","versionDate":"2026-06-17","versionId":"1","year":"2026","title":"Nereocystis luetkeana geographic patterns in physiology and microbiome from samples collected from 2019 to 2022 at nine sites spanning more than 200 kilometers in Washington state","authors":"Pfister, C. A.","publisher":"Biological and Chemical Oceanography Data Management Office (BCO-DMO)."},"doi":"10.26008/1912/bco-dmo.1000920.1","release_date":null,"field_dataset_location":"Washington state coastal waters","temporal_extent":{"start":"2019-07-02T04:00:00.000Z","end":"2022-08-14T04:00:00.000Z"},"temporal":{"resolution":"time:unitDay","type":"time:Interval","start":{"value":"1562040000","label":"2019-07-02"},"end":{"value":"1660449600","label":"2022-08-14"}},"spatial_extent":{"shape":"point","center":{"latitude":47.166522,"longitude":-124.73382},"wkt":" POINT (-124.73382 47.166522)","north":0,"east":0,"south":0,"west":0},"dataset_type":["Other Field Results"],"keywords":["oceans"],"data_files":[{"id":"1005309","title":"1000920_v1_nereocystis_geography.csv","url":"https://datadocs.bco-dmo.org/dataset/1000920/file/vmEANkvS79BJ9G/1000920_v1_nereocystis_geography.csv","showTableUrl":"https://www.bco-dmo.org/dataset/1000920/data/view/1005309","description":"Primary data file for dataset ID 1000920, version 1","filename":"1000920_v1_nereocystis_geography.csv","bytesize":25325,"checksum":{"value":"51d964e146b7272954ae3d11f98986e5","Algorithm":{"_id":"190","name":"MD5"}},"mimetype":"text/csv","fileType":{"_id":"192","name":"Comma Separated Values (.csv)"},"isErddap":"bcodmo_dataset_1000920_v1","date":"","isPublicDuringEmbargo":false,"primaryDataFile":true,"showTable":true,"restricted":false}],"supplemental_files":[],"roles":[{"role":{"_id":"138","name":"Principal Investigator"},"performedBy":{"_id":"474679","name":"Catherine Ann Pfister"},"performedAtOrganization":{"_id":"81","name":"University of Chicago"}},{"role":{"_id":"145","name":"BCO-DMO Data Manager"},"performedBy":{"_id":"51498","name":"Shannon Rauch"},"performedAtOrganization":{"_id":"191","name":"Woods Hole Oceanographic Institution"}}],"deployments":[],"funding":[{"_id":"917588","name":"OCE-2329475","fundingSource":{"_id":"355","name":"NSF Division of Ocean Sciences"},"url":"https://www.nsf.gov/awardsearch/show-award?AWD_ID=2329475"}],"instruments":[{"_id":"1001301","name":"Illumina MiSeq","description":"Sequences were generated from the Illumina MiSeq platform.","type_id":"649","type_name":"Automated DNA Sequencer","type_desc":"A DNA sequencer is an instrument that determines the order of deoxynucleotides in deoxyribonucleic acid sequences.","type_acronym":"Automated Sequencer"},{"_id":"1001287","name":"calipers","description":"All kelp measurements were made in situ, often via kayak or at the shore using a measuring tape and calipers.","type_id":"820156","type_name":"calipers","type_desc":"A caliper (or \"pair of calipers\") is a device used to measure the distance between two opposite sides of an object. Many types of calipers permit reading out a measurement on a ruled scale, a dial, or a digital display.","type_acronym":""},{"_id":"1001299","name":"Qiagen DNeasy PowerSoil Kit (Qiagen)","description":"DNA from the swabs was extracted with a Qiagen DNeasy PowerSoil Kit (Qiagen).","type_id":"1000452","type_name":"DNA Analysis Kit","type_desc":"A laboratory kit containing reagents and materials used to extract, purify, detect, measure, or otherwise evaluate DNA from a sample.","type_acronym":""},{"_id":"1001298","name":"Olympus BX50 fluorescence microscope","description":"Slides were visualized at 100× with an Olympus BX50 fluorescence microscope with a DAPI filter.","type_id":"695","type_name":"Fluorescence Microscope","type_desc":"Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments.","type_acronym":""},{"_id":"1001295","name":"pulse amplitude modulated (PAM) fluorometry in situ (Diving Pam, Walz, Germany)","description":"Using pulse amplitude modulated (PAM) fluorometry in situ (Diving Pam, Walz, Germany) we estimated fluorescence over a range of irradiances. PAM is a non-invasive, in situ method that quantifies Photosystem II fluorescence parameters across different light levels.","type_id":"484","type_name":"Fluorometer","type_desc":"A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ.","type_acronym":"Fluorometer"},{"_id":"1001288","name":"GenoGrinder Spex","description":"Kelp tissue was ground to a fine powder in a GenoGrinder Spex (Metuchen, NJ).","type_id":"522984","type_name":"Homogenizer","type_desc":"A homogenizer is a piece of laboratory equipment used for the homogenization of various types of material, such as tissue, plant, food, soil, and many others.","type_acronym":"Homogenizer "},{"_id":"1001290","name":"elemental analyser isotope ratio mass spectrometer","description":"Packed tins were analysed on an elemental analyser isotope ratio mass spectrometer at Northwestern University Stable Isotope Biogeochemistry Laboratory (NUSIBL).","type_id":"469","type_name":"Isotope-ratio Mass Spectrometer","type_desc":"The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer).","type_acronym":"IR Mass Spec; IRMS"},{"_id":"1001286","name":"measuring tape","description":"All kelp measurements were made in situ, often via kayak or at the shore using a measuring tape and calipers.","type_id":"645010","type_name":"Measuring Tape","type_desc":"A tape measure or measuring tape is a flexible ruler. It consists of a ribbon of cloth, plastic, fibre glass, or metal strip with linear-measurement markings. It is a common tool for measuring distance or length.","type_acronym":""},{"_id":"1001293","name":"optical oxygen and temperature meter (FireStingO2 FSO2-4)","description":"The change in oxygen was measured for 1 hour with a Pyro Science contactless fibre-optic sensors (Oxygen Sensor Spots OXSP5) and an optical oxygen and temperature meter (FireStingO2 FSO2-4), with the sensor spots affixed inside each 1 L chamber.","type_id":"705","type_name":"Oxygen Sensor","type_desc":"An electronic device that measures the proportion of oxygen (O2) in the gas or liquid being analyzed","type_acronym":"Dissolved Oxygen Sensor"},{"_id":"1001292","name":"Oxygen Sensor Spots (OXSP5)","description":"The change in oxygen was measured for 1 hour with a Pyro Science contactless fibre-optic sensors (Oxygen Sensor Spots OXSP5) and an optical oxygen and temperature meter (FireStingO2 FSO2-4), with the sensor spots affixed inside each 1 L chamber.","type_id":"923438","type_name":"Oxygen Sensor Spot","type_desc":"Oxygen sensor spots allow oxygen measurements in closed sample containers with contactless read-out through a transparent window (glass, acryl glass) with special adapters and optical fibers. These patches often have a self-adhesive option. ","type_acronym":"Oxygen Sensor Patch "},{"_id":"1001291","name":"Pesola scale","description":"The youngest basal frond tissue extending 24 to 30 cm from the bulb was weighed with a Pesola scale.","type_id":"714","type_name":"scale or balance","type_desc":"Devices that determine the mass or weight of a sample.","type_acronym":""},{"_id":"1001296","name":"Castaway-CTD (Sontek)","description":"The salinity and seawater temperatures were assessed with a Castaway-CTD (Sontek) from a boat or kayak at all sites except Tatoosh Island where instrumentation is moored (Hach DS5).","type_id":"893178","type_name":"SonTek CastAway-CTD","type_desc":"The Sontek CastAway-CTD (manufactured by Xylem) is a handheld castable instrument that provides instantaneous profiles of temperature, salinity, and sound speed. Each cast is referenced with both time and location using its built-in GPS receiver. The CastAway software displays profiles of the casts in addition to mapping the locations of the data collection points. The CastAway-CTD has a 5 Hz response and sampling rate, accurate to 0.1 (PSS-78), 0.05° Celsius.\r\n\r\nConductivity range is 0 to 100,000 µS/cm.\r\nTemperature range is -5° to 45° Celsius.\r\nPressure range is 0 to 100 decibars.\r\n\r\nFurther specs and information can be found on the manufacturer's website: https://www.xylem.com/en-us/brands/wtw/wtw-products/castaway-ctd/","type_acronym":"CastAway-CTD"},{"_id":"1001300","name":"polymerase chain reaction (PCR)","description":"The V4–V5 region within the 16S ribosomal RNA (rRNA) gene was amplified using universal bacterial primers and polymerase chain reaction (PCR) conditions.","type_id":"471582","type_name":"Thermal Cycler","type_desc":"A thermal cycler or \"thermocycler\" is a general term for a type of laboratory apparatus, commonly used for performing polymerase chain reaction (PCR), that is capable of repeatedly altering and maintaining specific temperatures for defined periods of time. The device has a thermal block with holes where tubes with the PCR reaction mixtures can be inserted. The cycler then raises and lowers the temperature of the block in discrete, pre-programmed steps. They can also be used to facilitate other temperature-sensitive reactions, including restriction enzyme digestion or rapid diagnostics.\r\n\r\n(adapted from http://serc.carleton.edu/microbelife/research_methods/genomics/pcr.html)","type_acronym":"Thermal Cycler"},{"_id":"1001297","name":"Hach DS5","description":"The salinity and seawater temperatures were assessed with a Castaway-CTD (Sontek) from a boat or kayak at all sites except Tatoosh Island where instrumentation is moored (Hach DS5).","type_id":"678","type_name":"Water Quality Multiprobe","type_desc":"An instrument which measures multiple water quality parameters based on the sensor configuration.","type_acronym":"Water Quality Multiprobe"}],"parameters":[{"_id":"1001312","name":"year","format":"%Y","frictionlessType":"date","description":"

Year

","units":{"_id":"","name":"unitless"},"type":{"_id":"1062","name":"year","description":"

year, reported as YYYY, e.g. 1995

"}},{"_id":"1001313","name":"date","format":"%Y-%m-%d","frictionlessType":"date","description":"

Date

","units":{"_id":"","name":"Unitless"},"type":{"_id":"1070","name":"date","description":"

date; generally reported in GMT as YYYYMMDD (year; month; day); also as MMDD (month; day); EqPac dates are local Hawaii time. ISO_Date format is YYYY-MM-DD (http://www.iso.org/iso/home/standards/iso8601.htm)

"}},{"_id":"1001314","name":"month","format":"","frictionlessType":"string","description":"

month

","units":{"_id":"","name":"unitless"},"type":{"_id":"1115","name":"month","description":"

Month of year; numeric 1 to 12.

"}},{"_id":"1001315","name":"locale","format":"","frictionlessType":"string","description":"

within coastal Washington state; see Methods

","units":{"_id":"","name":"unitless"},"type":{"_id":"1124","name":"site","description":"

Sampling site identification.

"}},{"_id":"1001316","name":"region","format":"","frictionlessType":"string","description":"

locale divided into 3 regions in coastal Washington state

","units":{"_id":"","name":"unitless"},"type":{"_id":"1124","name":"site","description":"

Sampling site identification.

"}},{"_id":"1001317","name":"latitude","format":"","frictionlessType":"number","description":"

Latitude

","units":{"_id":"","name":"decimal degrees"},"type":{"_id":"730","name":"latitude","description":"

latitude, in decimal degrees, North is positive, negative denotes South; Reported in some datasets as degrees, minutes

"}},{"_id":"1001319","name":"longitude","format":"","frictionlessType":"number","description":"

Longitude

","units":{"_id":"","name":"decimal degrees"},"type":{"_id":"731","name":"longitude","description":"

longitude, in decimal degrees, East is positive, negative denotes West; Reported in some datasets as degrees, minutes

"}},{"_id":"1001321","name":"replicate","format":"","frictionlessType":"string","description":"

per site per year

","units":{"_id":"","name":"unitless"},"type":{"_id":"953","name":"replicate","description":"

an identifier used to distinguish between one or more replicate samples

"}},{"_id":"1001322","name":"wetmass_g","format":"","frictionlessType":"number","description":"

wetmass of Nereocystis tissue used in estimating carbon fixation, reported in grams

","units":{"_id":"","name":"grams"},"type":{"_id":"886","name":"mass","description":"

mass; the amount of matter in an object.

"}},{"_id":"1001323","name":"drymass_g","format":"","frictionlessType":"number","description":"

drymass of Nereocystis tissue used in estimating carbon fixation, reported in grams

","units":{"_id":"","name":"grams"},"type":{"_id":"886","name":"mass","description":"

mass; the amount of matter in an object.

"}},{"_id":"1001324","name":"mgCfixed_gdrymass_hr","format":"","frictionlessType":"number","description":"

the mg of Carbon fixed per g drymass of Nereocystis per hour

","units":{"_id":"","name":"milligrams per gram drymass per hour"},"type":{"_id":"707571","name":"C","description":"

Elemental carbon (C)

"}},{"_id":"1001325","name":"DOCmgl_release_gdrymass_hr_corr01","format":"","frictionlessType":"number","description":"

the mg of dissolved organic Carbon released per g drymass of Nereocystis per hour, with 0.01 added to all values to allow analyses of log values for all and to avoid dividing by zero (see below)

","units":{"_id":"","name":"milligrams per gram drymass per hour"},"type":{"_id":"1188","name":"DOC","description":"

dissolved organic Carbon

"}},{"_id":"1001326","name":"DOC_um_hr_drymass","format":"","frictionlessType":"number","description":"

the uM of Carbon dissolved organic Carbon released per g drymass of Nereocystis per hour

","units":{"_id":"","name":"micromolar (uM)"},"type":{"_id":"1188","name":"DOC","description":"

dissolved organic Carbon

"}},{"_id":"1001327","name":"to_po4","format":"","frictionlessType":"number","description":"

the initial P04 concentration in uM

","units":{"_id":"","name":"micromolar (uM)"},"type":{"_id":"820747","name":"phosphate","description":"

Phosphate (PO43-)

"}},{"_id":"1001328","name":"to_si","format":"","frictionlessType":"number","description":"

the initial SiO4 concentration in uM

","units":{"_id":"","name":"micromolar (uM)"},"type":{"_id":"1209","name":"SiO4","description":"

Silicate; reactive silica. Also referred to as dissolved silicate, dissolved silica.

"}},{"_id":"1001329","name":"to_no3","format":"","frictionlessType":"number","description":"

the initial NO3 concentration in uM

","units":{"_id":"","name":"micromolar (uM)"},"type":{"_id":"1194","name":"NO3","description":"

Nitrate concentration in the water column

"}},{"_id":"1001330","name":"to_no2","format":"","frictionlessType":"number","description":"

the initial NO2 concentration in uM

","units":{"_id":"","name":"micromolar (uM)"},"type":{"_id":"1192","name":"NO2","description":"

Nitrite

"}},{"_id":"1001331","name":"to_nh4","format":"","frictionlessType":"number","description":"

the initial NH4 concentration in uM

","units":{"_id":"","name":"micromolar (uM)"},"type":{"_id":"901","name":"Ammonium","description":"

Ammonium and ammonia concentration parameters in any body of fresh or salt water.

"}},{"_id":"1001332","name":"to_DIN","format":"","frictionlessType":"number","description":"

the initial dissolved inorganic nitrogen concentration in uM

","units":{"_id":"","name":"micromolar (uM)"},"type":{"_id":"540831","name":"Dissolved Inorganic Nitrogen","description":"

Dissolved Inorganic Nitrogen per unit volume of the water

"}},{"_id":"1001333","name":"uM_DOC_to","format":"","frictionlessType":"number","description":"

the initial dissolved organic carbon concentration in uM

","units":{"_id":"","name":"micromolar (uM)"},"type":{"_id":"1188","name":"DOC","description":"

dissolved organic Carbon

"}},{"_id":"1001334","name":"tf_po4","format":"","frictionlessType":"number","description":"

the final P04 concentration in uM

","units":{"_id":"","name":"micromolar (uM)"},"type":{"_id":"820747","name":"phosphate","description":"

Phosphate (PO43-)

"}},{"_id":"1001335","name":"tf_si","format":"","frictionlessType":"number","description":"

the final SiO4 concentration in uM

","units":{"_id":"","name":"micromolar (uM)"},"type":{"_id":"1209","name":"SiO4","description":"

Silicate; reactive silica. Also referred to as dissolved silicate, dissolved silica.

"}},{"_id":"1001336","name":"tf_no3","format":"","frictionlessType":"number","description":"

tf_no3 - the final NO3 concentration in uM

","units":{"_id":"","name":"micromolar (uM)"},"type":{"_id":"1194","name":"NO3","description":"

Nitrate concentration in the water column

"}},{"_id":"1001337","name":"tf_no2","format":"","frictionlessType":"number","description":"

tf_no2 - the final NO2 concentration in uM

","units":{"_id":"","name":"micromolar (uM)"},"type":{"_id":"1192","name":"NO2","description":"

Nitrite

"}},{"_id":"1001338","name":"tf_nh4","format":"","frictionlessType":"number","description":"

tf_nh4 - the final NH4 concentration in uM

","units":{"_id":"","name":"micromolar (uM)"},"type":{"_id":"901","name":"Ammonium","description":"

Ammonium and ammonia concentration parameters in any body of fresh or salt water.

"}},{"_id":"1001339","name":"tf_DIN","format":"","frictionlessType":"number","description":"

tf_DIN - the final dissolved inorganic nitrogen concentration in uM

","units":{"_id":"","name":"micromolar (uM)"},"type":{"_id":"540831","name":"Dissolved Inorganic Nitrogen","description":"

Dissolved Inorganic Nitrogen per unit volume of the water

"}},{"_id":"1001340","name":"no3_uptake_h_gdm","format":"","frictionlessType":"number","description":"

no3_uptake_h_gdm - the uM change in NO3 per hour per gram drymass of Nereocystis

","units":{"_id":"","name":"uM change per gram drymass per hour"},"type":{"_id":"1194","name":"NO3","description":"

Nitrate concentration in the water column

"}},{"_id":"1001341","name":"bulb_diam","format":"","frictionlessType":"number","description":"

bulb_diam - widest part of bulb, in mm

","units":{"_id":"","name":"millimeters (mm)"},"type":{"_id":"1959","name":"diameter","description":"

Diameter is defined as the length of a straight line passing through the center of a round object or figure from side to side.

"}},{"_id":"1001342","name":"blade_length","format":"","frictionlessType":"number","description":"

blade_length - blade length used in assay, in cm

","units":{"_id":"","name":"centimeters (cm)"},"type":{"_id":"1097","name":"length","description":"

Length is a one-dimensional extent equal to the distance between two points.

"}},{"_id":"1001343","name":"repro","format":"","frictionlessType":"string","description":"

indication of whether kelp is reproductive (R) or not (NR)

","units":{"_id":"","name":"unitless"},"type":{"_id":"1741","name":"sample_descrip","description":"

text description or other traits of the sample collected

"}},{"_id":"1001344","name":"deln","format":"","frictionlessType":"number","description":"

the delta 15N, stable isotope ratio of Nitrogen, in Nereocystis tissue

","units":{"_id":"","name":"per mil"},"type":{"_id":"559085","name":"d15N_bio","description":"

delta 15N (d15N) measurements on living (at the time of sampling) organisms or parts thereof. d15N is a measure of the ratio of stable isotopes 15N:14N, reported in parts per thousand (per mil, ‰).

"}},{"_id":"1001345","name":"delc","format":"","frictionlessType":"number","description":"

delc - the delta 13C, stable isotope ratio of Carbon, in Nereocystis tissue

","units":{"_id":"","name":"per mil"},"type":{"_id":"559084","name":"d13C_bio","description":"

delta 13C (d13C) measurements on living (at the time of sampling) organisms or parts thereof. d13C is a measure of the ratio of stable isotopes 13C:12C, reported in parts per thousand (per mil, ‰).

"}},{"_id":"1001346","name":"pctn","format":"","frictionlessType":"number","description":"

pctn - the percent of tissue nitrogen (as dry mass) in Nereocystis tissue

","units":{"_id":"","name":"percent"},"type":{"_id":"707573","name":"N","description":"

Nitrogen (N)

"}},{"_id":"1001347","name":"pctc","format":"","frictionlessType":"number","description":"

pctc - the percent of tissue carbon (as dry mass) in Nereocystis tissue

","units":{"_id":"","name":"percent"},"type":{"_id":"707571","name":"C","description":"

Elemental carbon (C)

"}},{"_id":"1001348","name":"c_n","format":"","frictionlessType":"number","description":"

c_n - the ratio of tissue carbon to tissue nitrogen (pctc/pctn)

","units":{"_id":"","name":"ratio"},"type":{"_id":"1186","name":"C_to_N","description":"

Carbon to Nitrogen ratio (C:N)

"}},{"_id":"1001349","name":"temperature","format":"","frictionlessType":"number","description":"

temperature - in deg C

","units":{"_id":"","name":"degrees Celsius"},"type":{"_id":"733","name":"temperature","description":"

water temperature at measurement depth

"}},{"_id":"1001350","name":"salinity","format":"","frictionlessType":"number","description":"

salinity - in ppt

","units":{"_id":"","name":"ppt"},"type":{"_id":"955","name":"sal","description":"

Salinity: The amount (mass or quantity of matter) of salt per amount (volume or mass) of a body of water.

"}},{"_id":"1001351","name":"ratioCfixtoDOCrel","format":"","frictionlessType":"number","description":"

ratioCfixtoDOCrel - the ratio of inorganic carbon fixed to dissolved organic carbon released (mcCfixed_gdrymasshr/DOCmgl_release_gdrymass_hr_corr01)

","units":{"_id":"","name":"ratio"},"type":{"_id":"833208","name":"ratio","description":"

A ratio is the relative size of two quantities expressed as the quotient of one divided by the other. The numbers in a ratio may be quantities or measurements of any kind, such as counts, lengths, weights, or concentrations, for example. When two quantities are measured with the same unit, as is often the case, their ratio is a dimensionless number.

"}}],"related_publications":[{"id":"1001357","citation":"Hasegawa, Y., Mark Welch, J. L., Rossetti, B. J., & Borisy, G. G. (2017). Preservation of three-dimensional spatial structure in the gut microbiome. Plos One, 12(11), e0188257. https://doi.org/10.1371/journal.pone.0188257\r\n","relation_type":["Methods"],"relation_desc":""},{"id":"926480","citation":"Intergovernmental Oceanographic Commission (1994) Protocols for the Joint Global Ocean Flux Study (JGOFS) Core Measurements. Paris, France, UNESCO-IOC, 170pp. (Intergovernmental Oceanographic Commission Manuals and Guides: 29), (JGOFS Report; 19). DOI: https://doi.org/10.25607/OBP-1409\r\n\r\n\r\n","relation_type":["Methods"],"relation_desc":""},{"id":"1001359","citation":"Jassby, A. D., & Platt, T. (1976). Mathematical formulation of the relationship between photosynthesis and light for phytoplankton. Limnology and Oceanography, 21(4), 540–547. Portico. https://doi.org/10.4319/lo.1976.21.4.0540\r\n","relation_type":["Methods"],"relation_desc":""},{"id":"1001358","citation":"McCoy, S. J., Santillán‐Sarmiento, A., Brown, M. T., Widdicombe, S., & Wheeler, G. L. (2019). Photosynthetic Responses of Turf‐forming Red Macroalgae to High <scp>CO</scp><sub>2</sub> Conditions. Journal of Phycology, 56(1), 85–96. Portico. https://doi.org/10.1111/jpy.12922\r\n","relation_type":["Methods"],"relation_desc":""},{"id":"1001354","citation":"Pfister, C. A., Altabet, M. A., & Weigel, B. L. (2019). Kelp beds and their local effects on seawater chemistry, productivity, and microbial communities. Ecology, 100(10). Portico. https://doi.org/10.1002/ecy.2798\r\n","relation_type":["Methods"],"relation_desc":""},{"id":"1001353","citation":"Pfister, C. A., Stanfield, E., Bogan, M., Weigel, B. L., Volbrecht, S., & Scorza, K. (2025). Foundational kelp species reveal links between host traits, the environment and the associated microbial community. Royal Society Open Science, 12(10). https://doi.org/10.1098/rsos.250637\r\n","relation_type":["Results"],"relation_desc":""},{"id":"873614","citation":"Wootton, J. T., Pfister, C. A., & Forester, J. D. (2008). Dynamic patterns and ecological impacts of declining ocean pH in a high-resolution multi-year dataset. Proceedings of the National Academy of Sciences, 105(48), 18848–18853. https://doi.org/10.1073/pnas.0810079105\r\n","relation_type":["Methods"],"relation_desc":""},{"id":"1001356","citation":"Younker, I. T., Molnar, N., Scorza, K., Weed, R., Light, S. H., & Pfister, C. A. (2024). Bacteria on the foundational kelp in kelp forest ecosystems: Insights from culturing, whole genome sequencing and metabolic assays. Environmental Microbiology Reports, 16(3). Portico. https://doi.org/10.1111/1758-2229.13270\r\n","relation_type":["Methods"],"relation_desc":""}],"related_datasets":[{"_id":"","name":"","url":"http://www.ncbi.nlm.nih.gov/bioproject/PRJNA1301461","relation_type":"IsRelatedTo","type_desc":"The dataset is related to another dataset.","relation_desc":"Data on the microbial taxa present are archived with NCBI PRJNA1301461","citation":"University of Chicago. Nereocystis luetkeana blade bacteria 16S 2022. 2025/08. In: BioProject [Internet]. Bethesda, MD: National Library of Medicine (US), National Center for Biotechnology Information; 2011-. Available from: http://www.ncbi.nlm.nih.gov/bioproject/PRJNA1301461. 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