http://lod.bco-dmo.org/id/dataset/1001113
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2026-06-16
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Solid Phase extracted metal distributions collected on the US GEOTRACES GP17- ANT expedition aboard R/V Nathaniel B. Palmer (cruise NBP24-01) in the Southern Ocean across the Amundsen Sea from December 2023 to January 2024
2026-06-16
publication
2026-06-16
revision
Woods Hole Oceanographic Institution (WHOI BCO-DMO)
2026-06-18
publication
https://doi.org/10.26008/1912/bco-dmo.1001113.1
Rene Maurice Boiteau
University of Minnesota
principalInvestigator
Daniel J. Repeta
Woods Hole Oceanographic Institution
principalInvestigator
Anil Timilsina
University of Minnesota
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Boiteau, R. M., Repeta, D. J., Coffey, N. R., Timilsina, A. (2026) Solid Phase extracted metal distributions collected on the US GEOTRACES GP17- ANT expedition aboard R/V Nathaniel B. Palmer (cruise NBP24-01) in the Southern Ocean across the Amundsen Sea from December 2023 to January 2024. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-06-16 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.1001113.1 [access date]
GP17-ANT DSPE Metal Concentrations Dataset Description: Methods and Sampling: <p><strong>Sample collection and preparation:</strong><br />
Each water sample was filtered directly from the trace metal clean GTC rosette/Go-Flo bottle sampler through a 0.45 micrometer (µm) Supor membrane filter (25 millimeters (mm)) or 0.2 µm Pall Acropak-200 Supor cartridge into a trace metal grade acid-cleaned 4-liter (L) polycarbonate bottles. Samples for solid phase extracted trace element ligands (TELs) were pumped at 20 milliliters per minute (mL/min) through Bond-Elut ENV solid phase extraction (SPE) columns (1 gram (g), 6 mL, P/N 12255012, Agilent Technologies) that had been previously activated by passing 6 mL each of distilled methanol (MeOH, Optima LCMS grade, Fisher Scientific), 0.1% HCl (VWR chemical, Aristar Ultra grade), and ultrapure water (qH₂O, 18.2 MΩ) through the column. SPE columns were frozen (-20 degrees Celsius (°C)) immediately after sample collection and returned to the laboratory for processing.</p>
<p><strong>Siderophore Processing:</strong><br />
SPE columns were thawed and concentrated TELs were then eluted with 10 mL distilled MeOH into 15 mL trace metal clean polypropylene tubes. Process blanks were prepared in parallel by eluting activated SPE columns with 10 mL MeOH. The methanol fraction was collected as the process blank. The eluted sample was concentrated down to ~1000 microliters (µL) by vacuum centrifugal concentrator (CentrIVap Evaporating System, LABCONCO).</p>
<p>A 10 µL stock solution of 100 micromolar (µM) cyanocobalamin was added to each sample as an internal standard.</p>
<p><strong>Quantitative analyses of siderophores:</strong><br />
Chromatographic analyses were performed on a bioinert Dionex Ultimate 3000 liquid chromatograph (LC) system fitted with a loading pump, a low flow pump, and a 10-port switching valve. Full loop (50 µL) samples were injected using the 50 µL sample loop, then separated using a BEH C18 column (ACQUITY UPLC BEH C18, 130A<sup>o</sup>, 1.7 µm, 2.1 mm X 50 mm, P/N 186002350, Waters) connected with a 5 mm guard column (ACQUITY UPLC BEH C18 VanGuard, 130A<sup>o</sup>, 1.7 µm, 2.1 mm X 5 mm, P/N 186003975, Waters) by the low flow pump at 50 μL/min. 5 millimolar (mM) ammonium formate in Milli-Q water and LC-MS-grade methanol were used as solvents A and B, respectively. Samples were separated using a 40 minute gradient flow (95% A/ 5% B to 5% A/ 95% B) followed by 9 minute washout at 95% B. Meanwhile, the loading pump was used to supply an internal standard (4 parts per billion (ppb) Indium in 1% HNO3 as solvent C) at a constant rate and maintain 26% of total organic (LC-MS grade MeoH) flow by mixing loading pump flow (150 µL/min) and low flow (50 µL) post-column before infusing into the ICPMS.</p>
<p>The combined flow from the LC (200 μL/min) was analyzed using a Thermo Scientific iCAP Q ICPMS fitted with a perfluoroalkoxy micronebulizer (PFA-ST, Elemental Scientific), and a cyclonic spray chamber cooled to 0 °C. Measurements were made in kinetic energy discrimination (KED) mode, with a helium collision gas flow of 4-4.5 mL/min to minimize isobaric interferences. Oxygen was introduced into the sample carrier gas at 25 mL/min to prevent the formation of reduced organic deposits onto the ICPMS skimmer and sampling cones.</p>
<p>The metal detector response was calibrated using standards, 10 ppb, 20 ppb, 50 ppb, 100 ppb, and 200 ppb, prepared from a commercial stock solution (InorganicVentures). These standards were analyzed using a method similar to other samples, but no column was connected and eluted with 5% of solvent B and 95% of solvent A for five minutes. A plot of the metal peak areas against concentration yielded a linear relationship (r² ~0.99) for the response of the ICPMS detector as calibration curve. Calibrations and process blanks were made for every 10-20 samples analyzed, with only small changes (RSD ~30%) were observed in the slope of the calibration relationship over the course of the two years of sample analysis. Concentrations of TELs in each sample were calculated by integrating the metal peak area (detector response) of interested time range and using appropriate calibration curve for the peak area.</p>
Funding provided by NSF Antarctic Sciences (NSF ANT) Award Number: ANT-2410011 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2410011
completed
Rene Maurice Boiteau
University of Minnesota
612-625-6983
228 Smith Hall 207 Pleasant Street Se
Minneapolis
MN
55455
USA
rboiteau@umn.edu
pointOfContact
Daniel J. Repeta
Woods Hole Oceanographic Institution
508-289-2635
360 Woods Hole Rd. MS #51 Watson Laboratory
Woods Hole
MA
02543
USA
drepeta@whoi.edu
pointOfContact
Anil Timilsina
University of Minnesota
atimilsi@umn.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Station
GEOTRACES_ID
Cast
ISO_DateTime_UTC
Date
Time
Event
Latitude
Longitude
Depth
TotalFeLigands_56_DSPEENV_CONC_BOTTLE
HydrophilicFeLigands_56_DSPEENV_CONC_BOTTLE
HydrophobicFeLigands_56_DSPEENV_CONC_BOTTLE
TotalNiLigands_60_DSPEENV_CONC_BOTTLE
TotalCuLigands_63_DSPEENV_CONC_BOTTLE
TotalZnLigands_66_DSPEENV_CONC_BOTTLE
vacuum centrifugal concentrator (CentrIVap Evaporating System, LABCONCO)
GTC rosette/Go-Flo bottle sampler
Dionex Ultimate 3000 liquid chromatograph (LC) system
Thermo Scientific iCAP Q ICPMS
Bond-Elut ENV solid phase extraction (SPE) columns
theme
None, User defined
station
sample identification
cast
ISO_DateTime_UTC
date
time of day
event
latitude
longitude
depth
Iron
Nickel
Copper
Zinc
featureType
BCO-DMO Standard Parameters
Concentrator Device
GO-FLO Bottle
High-Performance Liquid Chromatograph
Inductively Coupled Plasma Mass Spectrometer
Solid Phase Extraction System
instrument
BCO-DMO Standard Instruments
NBP2401
service
Deployment Activity
Punta Arenas, Chile to Lyttelton, New Zealand
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
U.S. GEOTRACES
http://www.geotraces.org/
U.S. GEOTRACES
GEOTRACES is a SCOR sponsored program; and funding for program infrastructure development is provided by the U.S. National Science Foundation.
GEOTRACES gained momentum following a special symposium, S02: Biogeochemical cycling of trace elements and isotopes in the ocean and applications to constrain contemporary marine processes (GEOSECS II), at a 2003 Goldschmidt meeting convened in Japan. The GEOSECS II acronym referred to the Geochemical Ocean Section Studies To determine full water column distributions of selected trace elements and isotopes, including their concentration, chemical speciation, and physical form, along a sufficient number of sections in each ocean basin to establish the principal relationships between these distributions and with more traditional hydrographic parameters;
* To evaluate the sources, sinks, and internal cycling of these species and thereby characterize more completely the physical, chemical and biological processes regulating their distributions, and the sensitivity of these processes to global change; and
* To understand the processes that control the concentrations of geochemical species used for proxies of the past environment, both in the water column and in the substrates that reflect the water column.
GEOTRACES will be global in scope, consisting of ocean sections complemented by regional process studies. Sections and process studies will combine fieldwork, laboratory experiments and modelling. Beyond realizing the scientific objectives identified above, a natural outcome of this work will be to build a community of marine scientists who understand the processes regulating trace element cycles sufficiently well to exploit this knowledge reliably in future interdisciplinary studies.
Expand "Projects" below for information about and data resulting from individual US GEOTRACES research projects.
U.S. GEOTRACES
largerWorkCitation
program
US GEOTRACES GP17 Section: Amundsen Sea Sector of the Antarctic Continental Margin (GP17-ANT)
https://www.geotraces.org/
US GEOTRACES GP17 Section: Amundsen Sea Sector of the Antarctic Continental Margin (GP17-ANT)
<p>U.S. GEOTRACES extended its meridional transect, initiated on the 2018 GP15 Alaska-Tahiti expedition, south to the Antarctic ice edge and then east to Chile with GP17-OCE (December 2022 - January 2023). Because of the potentially important trace elements and isotopes (TEIs) inputs and transformations occurring in Antarctic waters and shelves, GP17 also had a second leg, GP17-ANT (November 29, 2023 - January 30, 2024) into coastal and shelf waters of Antarctica’s Amundsen Sea. Further information is available on the <a href="https://usgeotraces.ldeo.columbia.edu/content/gp17-ant" target="_blank">US GEOTRACES website</a>.</p>
<p><em>NSF Project Title:</em> Collaborative Research: Management and Implementation of US GEOTRACES GP17 Section: Amundsen Sea Sector of the Antarctic Continental Margin (GP17-ANT)</p>
<p><em>NSF Award Abstract:</em><br />
This project will support the management and implementation of a 60-day research cruise to the Amundsen Sea sector of the Antarctic continental margin to collect samples for measurements of a broad suite of trace elements and isotopes ('TEIs'), as part of the U.S. GEOTRACES program. GEOTRACES is a global effort in the field of Chemical Oceanography, the goal of which is to understand the distributions of trace elements and their isotopes in the ocean. Determining the distributions of these elements and isotopes will increase the understanding of processes that shape their distributions and also the processes that depend on these elements. Key TEIs include essential micronutrients such as iron and zinc; 'tracers' such as aluminum, manganese, and isotopes of nitrogen, thorium and neodymium that can be used to investigate modern and ancient ocean processes; and elements such as lead that are indicative of human activities. In the Southern Ocean, the Antarctic continental margins are important as sources of micronutrient trace elements such as iron, which is required to support biological production and carbon export over the Antarctic shelf and in offshore waters of the Antarctic Circumpolar Current. Moreover, these regions are experiencing rapid environmental changes that are expected to impact oceanic circulation and biogeochemical cycles, for which TEIs provide crucial data needed to test and refine numerical models of the Earth system. The Amundsen Sea sector holds particular interest because of the pronounced, decadal-scale increases in the melting rates of glacial ice shelves that border the region, driven by intrusions of warm Circumpolar Deep Water onto the continental shelf. This melting has potentially major impacts on global sea level, on the formation of Antarctic Bottom Water in the Ross Sea, and on the regional ecosystem.</p>
<p>The cruise will comprise essential sampling operations (collection and shipboard processing) and ancillary measurements (hydrography, nutrients, algal pigments) in support of multiple, individual science projects, following the successful model of previous U.S. GEOTRACES cruises in the Atlantic, Pacific and Arctic ocean basins. The cruise will sample the ocean region between 100°W and 135°W, with stations ranging from 67°S in the Antarctic Circumpolar Current southward to the Amundsen Sea continental shelf, including stations adjacent to several rapidly melting ice shelves and in highly-productive shelf polynyas. Water column samples will be collected using conventional and trace-metal clean CTD-rosette systems, in-situ high-volume pumps, and a towed fish sampler or small boat, using established methods. Sampling time will also be provided for collection of sea ice, floating glacial ice, and seafloor sediments. To facilitate coordination with a complementary open-ocean cruise and ensure access to the study region to document the impact of biological processes, the cruise is planned for late austral summer (late January-late March). Beyond the disciplinary contributions, the proposed research will contribute knowledge concerning the cryosphere and its impacts on global sea level and ocean circulation, regional ecosystems and biological processes, ocean-atmosphere interactions, and past and future environmental change. The project will contribute to STEM education and outreach through the participation of an NSF-funded PolarTREC education professional, and a K-12 STEM program for students from underserved and underrepresented schools run by Rutgers University education specialists. To foster public engagement, the investigators will partner with the UCSC Science Communication Program to engage freelance science journalists to profile research in this spectacular and harsh Antarctic environment.</p>
GP17-ANT
largerWorkCitation
project
Collaborative Research: US GEOTRACES GP-17-ANT: Molecular speciation of trace element-ligand complexes in the Southern Ocean and Antarctic shelf
https://osprey.bco-dmo.org/project/955348
Collaborative Research: US GEOTRACES GP-17-ANT: Molecular speciation of trace element-ligand complexes in the Southern Ocean and Antarctic shelf
<p><em>NSF Award Abstract:</em><br />
The supply of iron and other biologically essential trace elements to surface waters is a critical regulator of biological growth in the Southern Ocean. Sea ice volume and the physical dynamics of the Southern Ocean are currently experiencing rapid changes. An understanding of the corresponding changes in metal cycling is needed as these elements are essential for the growth of phytoplankton which plays an important role in modulating atmospheric CO2. One of the major knowledge gaps is understanding how soluble forms of these metals are generated and transported to the surface ocean. Many trace elements are insoluble in seawater and precipitate close to their sources unless they are bound to a dissolved organic molecule, or ligand, that keeps the metal in solution. Little is currently known about what these ligands are, where they come from, or how they affect the reactivity and fate of metals in the Southern Ocean. The study proposed here is designed to identify the organic ligands that bind to metals and determine the ligand sources and reactivity along the Antarctic continental margin.</p>
<p>The proposed project will survey the molecular speciation of six biologically important trace elements (Fe, Cu, Zn. Ni, Co, Mn) along the US GEOTRACES cruise track along the Antarctic shelf in the Amundsen Sea in order to better understand trace element cycling across the region. The goal of the GEOTRACES expedition is to identify processes and quantify fluxes that control the distributions of trace elements and their isotopes to the Southern Ocean. As part of this effort, the investigators will assess biological ligand production as the ecosystem shifts from low productivity iron-starved conditions in the Antarctic Circumpolar Current to higher productivity along the upwelling continental margin, with a particular focus on assessing production within the Amundsen Sea polynya blooms and sinking organic matter remineralization zones. Second, changes in the accumulation, saturation, and composition of ligands in Circumpolar Deep Water will be measured as the water passes across the Antarctic shelf and receives inputs from sediments and glacial meltwaters. Finally, the role of sea ice on metal-ligand dynamics will be investigated. Metal ligands are a central parameter of numerical ocean models that predict and estimate metal distributions, and results from this project will provide those models with knowledge of the processes that supply ligands and affect ligand concentrations. Two graduate students and undergraduate interns will be supported and trained as part of this project.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
GP17-ANT Trace element-ligand complexes
largerWorkCitation
project
eng; USA
oceans
Punta Arenas, Chile to Lyttelton, New Zealand
-133.02246
-99.68365
-74.38082
-66.97744
2023-12-08
2024-01-13
From projects that focused on the following 2 locations: 1. Amundsen Sea Sector of the Antarctic Continental Margin 2. Southern Ocean
0
BCO-DMO catalogue of parameters from Solid Phase extracted metal distributions collected on the US GEOTRACES GP17- ANT expedition aboard R/V Nathaniel B. Palmer (cruise NBP24-01) in the Southern Ocean across the Amundsen Sea from December 2023 to January 2024
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/1001131.rdf
Name: Station
Units: Unitless
Description: <p>Cruise station identifier</p>
http://lod.bco-dmo.org/id/dataset-parameter/1001132.rdf
Name: GEOTRACES_ID
Units: Unitless
Description: <p>Unique GEOTRACES identifier for each water-column sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/1001133.rdf
Name: Cast
Units: Unitless
Description: <p>Cast number</p>
http://lod.bco-dmo.org/id/dataset-parameter/1001134.rdf
Name: ISO_DateTime_UTC
Units: units
Description: <p>Sample collection date and time (UTC) in ISO 8601 format</p>
http://lod.bco-dmo.org/id/dataset-parameter/1001136.rdf
Name: Date
Units: Unitless
Description: <p>Sample collection date</p>
http://lod.bco-dmo.org/id/dataset-parameter/1001137.rdf
Name: Time
Units: Unitless
Description: <p>Sample collection time (UTC)</p>
http://lod.bco-dmo.org/id/dataset-parameter/1001138.rdf
Name: Event
Units: Unitless
Description: <p>Event number</p>
http://lod.bco-dmo.org/id/dataset-parameter/1001139.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude of sampling event; negative = south</p>
http://lod.bco-dmo.org/id/dataset-parameter/1001141.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude of sampling event; negative = west</p>
http://lod.bco-dmo.org/id/dataset-parameter/1001143.rdf
Name: Depth
Units: meter (m)
Description: <p>Sample collection depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/1001145.rdf
Name: TotalFeLigands_56_DSPEENV_CONC_BOTTLE
Units: nanomole per litre (nM)
Description: <p>Total SPE dissolved iron concentration in nM [integrated retention time interval 6-50 min]</p>
http://lod.bco-dmo.org/id/dataset-parameter/1001146.rdf
Name: HydrophilicFeLigands_56_DSPEENV_CONC_BOTTLE
Units: nanomole per litre (nM)
Description: <p>Hydrophilic SPE dissolved iron concentration in nM [integrated retention time interval 6-33 min]</p>
http://lod.bco-dmo.org/id/dataset-parameter/1001147.rdf
Name: HydrophobicFeLigands_56_DSPEENV_CONC_BOTTLE
Units: nanomole per litre (nM)
Description: <p>Hydrophobic SPE dissolved iron concentration in nM [integrated retention time interval 33-50 min]</p>
http://lod.bco-dmo.org/id/dataset-parameter/1001148.rdf
Name: TotalNiLigands_60_DSPEENV_CONC_BOTTLE
Units: nanomole per litre (nM)
Description: <p>Total SPE dissolved Nickel concentration in nM [integrated retention time interval 6-50 min]</p>
http://lod.bco-dmo.org/id/dataset-parameter/1001149.rdf
Name: TotalCuLigands_63_DSPEENV_CONC_BOTTLE
Units: nanomole per litre (nM)
Description: <p>Total SPE dissolved Copper concentration in nM [integrated retention time interval 6-50 min]</p>
http://lod.bco-dmo.org/id/dataset-parameter/1001150.rdf
Name: TotalZnLigands_66_DSPEENV_CONC_BOTTLE
Units: nanomole per litre (nM)
Description: <p>Total SPE dissolved Zinc concentration in nM [integrated retention time interval 6-50 min]</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://doi.org/10.26008/1912/bco-dmo.1001113.1
download
onLine
dataset
<p><strong>Sample collection and preparation:</strong><br />
Each water sample was filtered directly from the trace metal clean GTC rosette/Go-Flo bottle sampler through a 0.45 micrometer (µm) Supor membrane filter (25 millimeters (mm)) or 0.2 µm Pall Acropak-200 Supor cartridge into a trace metal grade acid-cleaned 4-liter (L) polycarbonate bottles. Samples for solid phase extracted trace element ligands (TELs) were pumped at 20 milliliters per minute (mL/min) through Bond-Elut ENV solid phase extraction (SPE) columns (1 gram (g), 6 mL, P/N 12255012, Agilent Technologies) that had been previously activated by passing 6 mL each of distilled methanol (MeOH, Optima LCMS grade, Fisher Scientific), 0.1% HCl (VWR chemical, Aristar Ultra grade), and ultrapure water (qH₂O, 18.2 MΩ) through the column. SPE columns were frozen (-20 degrees Celsius (°C)) immediately after sample collection and returned to the laboratory for processing.</p>
<p><strong>Siderophore Processing:</strong><br />
SPE columns were thawed and concentrated TELs were then eluted with 10 mL distilled MeOH into 15 mL trace metal clean polypropylene tubes. Process blanks were prepared in parallel by eluting activated SPE columns with 10 mL MeOH. The methanol fraction was collected as the process blank. The eluted sample was concentrated down to ~1000 microliters (µL) by vacuum centrifugal concentrator (CentrIVap Evaporating System, LABCONCO).</p>
<p>A 10 µL stock solution of 100 micromolar (µM) cyanocobalamin was added to each sample as an internal standard.</p>
<p><strong>Quantitative analyses of siderophores:</strong><br />
Chromatographic analyses were performed on a bioinert Dionex Ultimate 3000 liquid chromatograph (LC) system fitted with a loading pump, a low flow pump, and a 10-port switching valve. Full loop (50 µL) samples were injected using the 50 µL sample loop, then separated using a BEH C18 column (ACQUITY UPLC BEH C18, 130A<sup>o</sup>, 1.7 µm, 2.1 mm X 50 mm, P/N 186002350, Waters) connected with a 5 mm guard column (ACQUITY UPLC BEH C18 VanGuard, 130A<sup>o</sup>, 1.7 µm, 2.1 mm X 5 mm, P/N 186003975, Waters) by the low flow pump at 50 μL/min. 5 millimolar (mM) ammonium formate in Milli-Q water and LC-MS-grade methanol were used as solvents A and B, respectively. Samples were separated using a 40 minute gradient flow (95% A/ 5% B to 5% A/ 95% B) followed by 9 minute washout at 95% B. Meanwhile, the loading pump was used to supply an internal standard (4 parts per billion (ppb) Indium in 1% HNO3 as solvent C) at a constant rate and maintain 26% of total organic (LC-MS grade MeoH) flow by mixing loading pump flow (150 µL/min) and low flow (50 µL) post-column before infusing into the ICPMS.</p>
<p>The combined flow from the LC (200 μL/min) was analyzed using a Thermo Scientific iCAP Q ICPMS fitted with a perfluoroalkoxy micronebulizer (PFA-ST, Elemental Scientific), and a cyclonic spray chamber cooled to 0 °C. Measurements were made in kinetic energy discrimination (KED) mode, with a helium collision gas flow of 4-4.5 mL/min to minimize isobaric interferences. Oxygen was introduced into the sample carrier gas at 25 mL/min to prevent the formation of reduced organic deposits onto the ICPMS skimmer and sampling cones.</p>
<p>The metal detector response was calibrated using standards, 10 ppb, 20 ppb, 50 ppb, 100 ppb, and 200 ppb, prepared from a commercial stock solution (InorganicVentures). These standards were analyzed using a method similar to other samples, but no column was connected and eluted with 5% of solvent B and 95% of solvent A for five minutes. A plot of the metal peak areas against concentration yielded a linear relationship (r² ~0.99) for the response of the ICPMS detector as calibration curve. Calibrations and process blanks were made for every 10-20 samples analyzed, with only small changes (RSD ~30%) were observed in the slope of the calibration relationship over the course of the two years of sample analysis. Concentrations of TELs in each sample were calculated by integrating the metal peak area (detector response) of interested time range and using appropriate calibration curve for the peak area.</p>
Specified by the Principal Investigator(s)
<p>Raw intensity ICPMS data over time were exported in '.csv' format. We used a custom Python script (v3.10.9) to integrate peak areas and calculate concentrations based on an external calibration curve (link: <a href="https://github.com/tanil07/LC-ICPMS-integration-and-combining-with-metadata" target="_blank">https://github.com/tanil07/LC-ICPMS-integration-and-combining-with-metadata</a>; DOI: <a href="http://dx.doi.org/10.5281/zenodo.18486409" target="_blank">10.5281/zenodo.18486409</a>).</p>
Specified by the Principal Investigator(s)
- Loaded data from "GP17 ANT DSPEMETAL Concentration 02122026.xlsx" (sheet "combined_odvdata_all_with_meta") into the BCO-DMO processing system.
- Renamed column "GEOTRACES ID" to "GEOTRACES_ID".
- Combined Date (format "%Y/%m/%d") and Time (format "%H:%M:%S") fields in UTC to create new datetime column "ISO_DateTime_UTC" (format "%Y-%m-%dT%H:%M:%SZ").
- Saved the final file as "1001113_v1_gp17_dspe_metal.csv".
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
vacuum centrifugal concentrator (CentrIVap Evaporating System, LABCONCO)
vacuum centrifugal concentrator (CentrIVap Evaporating System, LABCONCO)
PI Supplied Instrument Name: vacuum centrifugal concentrator (CentrIVap Evaporating System, LABCONCO) Instrument Name: Concentrator Device Instrument Short Name: Instrument Description: A concentrator is a device designed to increase the weight per unit volume of a substance.
This category includes vacuum centrifuge concentrator, which include a vacuum chamber within which a centrifuge rotor is mounted for spinning a plurality of vials containing a solution at high speed while subjecting the solution to a vacuum condition for concentration and evaporation. Alternative names: sample concentrator; speed vacuum; speed vac.
GTC rosette/Go-Flo bottle sampler
GTC rosette/Go-Flo bottle sampler
PI Supplied Instrument Name: GTC rosette/Go-Flo bottle sampler Instrument Name: GO-FLO Bottle Instrument Short Name:GO-FLO Instrument Description: GO-FLO bottle cast used to collect water samples for pigment, nutrient, plankton, etc. The GO-FLO sampling bottle is specially designed to avoid sample contamination at the surface, internal spring contamination, loss of sample on deck (internal seals), and exchange of water from different depths. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/30/
Dionex Ultimate 3000 liquid chromatograph (LC) system
Dionex Ultimate 3000 liquid chromatograph (LC) system
PI Supplied Instrument Name: Dionex Ultimate 3000 liquid chromatograph (LC) system Instrument Name: High-Performance Liquid Chromatograph Instrument Short Name:HPLC Instrument Description: A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB11/
Thermo Scientific iCAP Q ICPMS
Thermo Scientific iCAP Q ICPMS
PI Supplied Instrument Name: Thermo Scientific iCAP Q ICPMS Instrument Name: Inductively Coupled Plasma Mass Spectrometer Instrument Short Name:ICP Mass Spec Instrument Description: An ICP Mass Spec is an instrument that passes nebulized samples into an inductively-coupled gas plasma (8-10000 K) where they are atomized and ionized. Ions of specific mass-to-charge ratios are quantified in a quadrupole mass spectrometer. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB15/
Bond-Elut ENV solid phase extraction (SPE) columns
Bond-Elut ENV solid phase extraction (SPE) columns
PI Supplied Instrument Name: Bond-Elut ENV solid phase extraction (SPE) columns Instrument Name: Solid Phase Extraction System Instrument Short Name:SPE System Instrument Description: Solid-phase extraction (SPE) is a solid-liquid extractive technique, by which compounds that are dissolved or suspended in a liquid mixture are separated, isolated or purified, from other compounds in this mixture, according to their physical and chemical properties
Cruise: NBP2401
NBP2401
Community Standard Description
International Council for the Exploration of the Sea
RVIB Nathaniel B. Palmer
vessel
NBP2401
Peter N. Sedwick
Old Dominion University
https://www.bodc.ac.uk/resources/inventories/cruise_inventory/reports/nathanielbpalmer_nbp2401.pdf
Report describing NBP2401
Community Standard Description
International Council for the Exploration of the Sea
RVIB Nathaniel B. Palmer
vessel