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            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset/1001800.rdf" xlink:actuate="onRequest">Rock and sediment samples collected by ROV SuBastian during the Octopus Odyssey expeditions Fkt230602 and Fkt231202 aboard R/V Falkor (too) in Costa Rica (2023)</gmx:Anchor>
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                <gmx:Anchor xlink:href="http://orcid.org/0000-0001-7004-8649" xlink:title="ORCID" xlink:actuate="onRequest">Jorge Cortés-Núñez</gmx:Anchor>
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                <gmx:Anchor xlink:href="https://ror.org/02yzgww51" xlink:title="ROR ID" xlink:actuate="onRequest">Universidad de Costa Rica</gmx:Anchor>
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                <gmx:Anchor xlink:href="https://ror.org/0294hxs80" xlink:title="ROR ID" xlink:actuate="onRequest">California State University Los Angeles</gmx:Anchor>
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                <gmx:Anchor xlink:href="https://ror.org/02yzgww51" xlink:title="ROR ID" xlink:actuate="onRequest">Universidad de Costa Rica</gmx:Anchor>
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            <gco:CharacterString>Cite this dataset as: Sandoval, M. I., Ramírez, G., Semedo, M., Cortés-Núñez, J., Orcutt, B. N. (2026) Rock and sediment samples collected by ROV SuBastian during the Octopus Odyssey expeditions Fkt230602 and Fkt231202 aboard R/V Falkor (too) in Costa Rica (2023). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-07-02 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.1001800.1 [access date]</gco:CharacterString>
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        <gco:CharacterString>ROV rock and sediment samples Dataset Description:  Methods and Sampling: &amp;lt;p&amp;gt;Sampling was conducted across six main geological features in the Pampa Submarina region off the Pacific coast of Costa Rica, including several hills, knolls, a seamount, and the surrounding abyssal plain.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Push core sediment samples were collected in the abyssal plain where sufficient sediment thickness allowed coring. Rock samples were collected during ROV SuBastian dives when suitable outcrops were encountered. Both push core and rock samples were collected using the ROV’s manipulator arms.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Rock samples were labelled using the standard code used in the expedition (Cruise ID, followed by a sequential sample code used for each expedition, and then the type of sample (S for sediments, R for rocks); e.g. FKt230602-006-S). Samples were photographed on board following the standards methods used for rocks.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Samples from these collections were allocated to various biological, physical, geochemical, and ecotoxicological analyses. These included characterization of sediment, fungi, worms, protists, and meiofauna, physical sediment descriptions using smear slides, geochemistry, ‘omics-based taxonomic and functional assessment of microbiomes, Cd exposure ecotoxicological experiments and background metal concentration of the 0-10 cm layer, namely by quantifying Cu, Fe, Mn, Zn, Ni, Cr, Pb, Cd.&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/856235.rdf" xlink:title="OISE-2114593" xlink:actuate="onRequest">Funding provided by NSF Office of International Science and Engineering (NSF OISE) Award Number: OISE-2114593 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2114593</gmx:Anchor>
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            <gmx:Anchor xlink:href="http://orcid.org/0000-0001-7004-8649" xlink:title="ORCID" xlink:actuate="onRequest">Jorge Cortés-Núñez</gmx:Anchor>
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            <gmx:Anchor xlink:href="https://ror.org/02yzgww51" xlink:title="ROR ID" xlink:actuate="onRequest">Universidad de Costa Rica</gmx:Anchor>
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            <gmx:Anchor xlink:href="https://ror.org/0294hxs80" xlink:title="ROR ID" xlink:actuate="onRequest">California State University Los Angeles</gmx:Anchor>
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Description:
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                            <gco:CharacterString>&lt;p&gt;&lt;strong&gt;Brief Overview:&lt;/strong&gt;&lt;br /&gt;
The first Octopus Odyssey expedition took place from June 2 to June 21, 2023 on R/V Falkor (too). The second expedition, Octopus Odyssey (too) took place from December 2 to December 15, 2023. Both expeditions explored The Dorado Outcrop, one of Costa Rica's &quot;Off-Axis seamounts on the complex Cocos Plate. These two cruises featured early career training activities and international capacity-sharing elements that were integrated into the NSF-funded COBRA program. In addition to the NSF award, this project was also supported by Schmidt Ocean Institute, Blue Nature Alliance, and Bigelow Laboratory for Ocean Sciences.&lt;/p&gt;
&lt;p&gt;More information is available from Schmidt Ocean Institute at:&lt;br /&gt;
&lt;a href=&quot;https://schmidtocean.org/cruise/octopus-odyssey/&quot; target=&quot;_blank&quot;&gt;https://schmidtocean.org/cruise/octopus-odyssey/&lt;/a&gt;&lt;br /&gt;
and&lt;br /&gt;
&lt;a href=&quot;https://schmidtocean.org/cruise/octopus-odyssey-too/&quot; target=&quot;_blank&quot;&gt;https://schmidtocean.org/cruise/octopus-odyssey-too/&lt;/a&gt;&lt;/p&gt;
&lt;p&gt;&lt;strong&gt;Detailed Description:&lt;/strong&gt;&lt;br /&gt;
Seamount ecosystems support highly diverse animal communities on the seafloor and the surrounding ocean, yet the diversity, connectivity and ecosystem services of these environments is poorly understood. The Pacific Ocean margin of Costa Rica contains a range of seamount habitats, from the rough terrain of the southwestern margin to the sparser terrain of the northwest margin. While the southwestern terrain has previously been surveyed (including by R/V Falkor in 2019) and some seamount areas are already protected, far less is known about the ecosystems of the northwestern terrain. In 2013/2014 unique animal behaviors and hydrothermal venting were discovered using ROV Jason and HOV Alvin on a small feature in the northwestern terrain. Namely, extensive aggregations of octopus were observed at a place called the Dorado Outcrop, located in areas of diffuse venting of slightly warmed hydrothermal fluids. At the time of discovery, it was unclear if these aggregations could be considered nurseries, since no viable eggs were observed with brooding mothers.&lt;/p&gt;
&lt;p&gt;Two expeditions of the RV Falkor (too) were planned for 2023 to return to this region to ask new questions about the connection of life, rocks, and fluids around these seafloor features. The team wanted to answer questions, such as:&lt;/p&gt;
&lt;ul&gt;
&lt;li&gt;Are there viable octopus nurseries hosted on seamounts offshore Costa Rica?&lt;/li&gt;
&lt;li&gt;If yes, are the octopus nurseries active at a different time of year?&lt;/li&gt;
&lt;li&gt;Do octopus brooding in hydrothermal springs have different microbiomes as compared to other octopus, and are those microbiomes connected to the microbes in the hydrothermal springs or surrounding rocks?&lt;/li&gt;
&lt;li&gt;Are the hydrothermal spring fluids unique, representing different trends in fluid-rock-life reactions, or do they represent a single altered fluid?&lt;/li&gt;
&lt;li&gt;Are there seasonal trends in biodiversity on the seafloor or in the water above?&lt;/li&gt;
&lt;/ul&gt;
&lt;p&gt;In June of 2023, an international team traveled to this region aboard R/V Falkor (too) for the Octopus Odyssey Leg 1 expedition Fkt230602 with a major goal to determine if the eggs at the nursery were viable, as past expeditions to the outcrop had never seen evidence of developing embryos. From 2-21 June 2023, we conducted 14 dives with ROV SuBastian to explore six seafloor features (only one of which had ever been explored before), augmented by 13 full-water-column CTD Niskin Rosette casts and six multibeam surveys. We had roughly 229 hours of ROV operations in the water (172 hours on the seafloor + 57 hours of ascent/descent), resulting in 208 hours of video. The longest ROV dive was approximately 35 hours and the deepest depth of ROV exploration was 3178 m. We had 285 sampling events during the ROV dives: 150 primary biological specimens (plus associates), 66 sediment push cores, 28 ROV Niskin samples of bottom water, 13 squeezer fluid samples, 30 rock samples. This also included deployments of 22 different experiments planned for recovery in December 2023, and recovery of 2 experiments from the Dorado Outcrop deployed in 2014. We also conducted 31 video transects. Operations went very smoothly, although some transit between sites had to be diverted due to long line fishing in the area, and one medical evacuation required transit to port before returning to site. On the first ROV dive at the nursery in June, we witnessed baby octopus hatching, confirming our primary hypothesis that there are viable octopus nurseries in this region. We also found the fifth known octopus nursery in the world on a different seafloor feature 30 nautical miles away. Exploration of the six seafloor features on the expedition revealed an incredibly rich biodiversity and biogeography of life on ancient volcanoes offshore Costa Rica. We also documented additional evidence of the hydrogeology of the region – how water moves in, out, and through oceanic crust. This data can inform why volcanoes and earthquakes in Costa Rica vary as different types of seamounts and oceanic crust subducts beneath overriding plates.&lt;/p&gt;
&lt;p&gt;In December 2023, the Octopus Odyssey (too) Leg 2 team returned to this region on RV Falkor (too) on expedition Fkt231202 to ask new questions about biodiversity in the region and to recover experiments to track the hydrogeology of the area. From 1-15 December 2023, Octopus Odyssey (too) conducted twelve full-ocean depth ROV dives with ROV SuBastian, augmented by five full-ocean depth CTD Niskin Rosette casts, and multibeam operations resulting in 7416 km2 of coverage in Costa Rican waters. We had roughly 104 hours of ROV operations (55 hours on the seafloor + 49 hours of ascent/descent). This has resulted in approximately 141 hours of video. The longest ROV dive was a little over 16 hours and the deepest depth of ROV exploration was 3179 mbsl. We had 241 sampling events with the ROV in the water: 93 primary biological specimens, 14 sediment push cores, 21 ROV Niskin samples, 20 rock samples, and 51 fluid samples collected with a third-party SUPR sampler. On the ship, we collected an additional 66 secondary associate biological samples from primary specimens, bringing the total number of samples to 307 (this does not include subsamples). We also conducted 23 video transects. For the most part, our operations went according to schedule. No ROV operations were ended early due to operational issues, although one dive was aborted on launch due to a ground fault in a third-party instrument; this was quickly resolved and the dive restarted. One dive ended early due to a fishing long-line drifting towards the vessel; we recovered early then re-dove on the site after the long-line passed by. Communications with fishing boats and the fisheries ministry, enabled by the Berth-of-Opportunity Observer from Instituto Costarricense de Pesca y Acuacultura (INCOPESCA), helped prevent further issues in the area. The biggest finding of the return expedition was confirmation that the octopus nurseries offshore Costa Rica support baby octopus throughout the year, not just in the summer rainy season. Scientists onboard witnessed spectacular scenes of the first moments of life, as baby octopus emerged from their eggs, including traveling with one hatchling for an epic journey over 150 m up into the water. Immature eggs were also observed to have tiny octopus embryos inside. Having two expeditions to the same region in one year was essential for confirming this finding. Moreover, the seamounts offshore Costa Rica support at least four new species of deep-sea octopuses, based on the collection of specimens from both Octopus Odyssey expeditions in June and December 2023. This is an unprecedented biodiversity of octopus in this small area especially at these depths.&lt;/p&gt;
&lt;p&gt;Equally as important as achieving the scientific objectives was the objective to continue the theme of capacity sharing, early career development, and raising awareness of deep-sea heritage in Latin America. The international Octopus Odyssey and Octopus Odyssey (too) teams gathered to achieve collaborative co-production of knowledge and training with Costa Ricans, honoring the work in Costa Rica’s waters. Spanish-speaking scientists were given priority for dive lead watches to enable livestream narration in Spanish, and priority for leadership experience. Ship-to-shore engagements were also prioritized for Spanish-speaking audiences, particularly in Costa Rica. These efforts were intended to raise the profile of the deep-sea heritage in Costa Rica ahead of the 2024 UN Ocean Conference meeting taking place in Costa Rica in June 2024. Over 300 biological specimens collected on the two expeditions are archived at the Museum of Zoology at the University of Costa Rica, enabling current and future generations of students and researchers to develop expertise in regional deep-sea animals. It is likely that many of the specimens collected represent new species and new records of known species for the region. Rock and sediment samples collected on the expeditions are revolutionizing the understanding of the complex geological origins and processes occurring on this part of the seafloor. Surprisingly, initial analysis of microfossils in sediments reveals that seafloor sediments are millions-of-years old, indicating strong currents, dissolution and scouring. In addition, fossils of beaked whales were found on numerous outcrops. All microfossils and macrofossils are archived in the Paleontology collection at the Central American School of Geology at the University of Costa Rica for continued study, with additional mineralogical samples shared with the Global Marine Minerals Program at the U.S. Geological Society. Finally, bathymetric and subbottom profile mapping data conducted on the OctoOdyssey expeditions was used to define the diverse seafloor features in this region to then propose official names to GEBCO. This naming effort is being led by Costa Rican scientists in consultation with the Costa Rican Committee on Nomenclature; the new proposed names were unanimously approved by the committee and will now be included on Costa Rican maps.&lt;/p&gt;</gco:CharacterString>
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            <gmd:MD_ScopeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MD_ScopeCode" codeListValue="dataset">dataset</gmd:MD_ScopeCode>
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                <gco:CharacterString>&amp;lt;p&amp;gt;Sampling was conducted across six main geological features in the Pampa Submarina region off the Pacific coast of Costa Rica, including several hills, knolls, a seamount, and the surrounding abyssal plain.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Push core sediment samples were collected in the abyssal plain where sufficient sediment thickness allowed coring. Rock samples were collected during ROV SuBastian dives when suitable outcrops were encountered. Both push core and rock samples were collected using the ROV’s manipulator arms.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Rock samples were labelled using the standard code used in the expedition (Cruise ID, followed by a sequential sample code used for each expedition, and then the type of sample (S for sediments, R for rocks); e.g. FKt230602-006-S). Samples were photographed on board following the standards methods used for rocks.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Samples from these collections were allocated to various biological, physical, geochemical, and ecotoxicological analyses. These included characterization of sediment, fungi, worms, protists, and meiofauna, physical sediment descriptions using smear slides, geochemistry, ‘omics-based taxonomic and functional assessment of microbiomes, Cd exposure ecotoxicological experiments and background metal concentration of the 0-10 cm layer, namely by quantifying Cu, Fe, Mn, Zn, Ni, Cr, Pb, Cd.&amp;lt;/p&amp;gt;</gco:CharacterString>
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                <gco:CharacterString>&amp;lt;p&amp;gt;Sample metadata were compiled and verified using the cruise data log maintained during both cruises. After each cruise, the dataset was reviewed to ensure consistency in sample codes, description, and location details. The information from both expeditions was then integrated into a single dataset.&amp;lt;/p&amp;gt;</gco:CharacterString>
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              <gmd:source>
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          </gmd:processStep>
        <gmd:processStep xlink:title="BCO-DMO Data Processing Description">
              <gmd:LI_ProcessStep>
                <gmd:description>
                  <gco:CharacterString>- Loaded original file &amp;quot;OctopusOdyssey_GeologicalSamples.csv&amp;quot; into the BCO-DMO system.
- Combined columns &amp;quot;eventDate&amp;quot; (time, format &amp;quot;%H:%M:%S&amp;quot;) and &amp;quot;eventDate2&amp;quot; (date, format &amp;quot;%Y-%m-%d&amp;quot;) into new datetime column &amp;quot;ISO_DateTime_UTC&amp;quot; formatted as &amp;quot;%Y-%m-%dT%H:%M:%SZ&amp;quot; in UTC.
- Applied find/replace on an erroneous &amp;quot;decimalLongitude&amp;quot; value, replacing &amp;quot;-87277339.0&amp;quot; with &amp;quot;-87.277339&amp;quot;.
- Saved the file as &amp;quot;1001800_v1_rov_rock_sediment_samples.csv&amp;quot; and requested submitter approval.
- Loaded the submitter-modified version of this file, &amp;quot;1001800_v1_rov_rock_sediment_samples_MS.csv&amp;quot; into the BCO-DMO system.
- Converted eventDate2 from format %d/%m/%Y to date type with output format %Y-%m-%d.
- Saved the final file as &amp;quot;1001800_v1_rov_rock_sediment_samples.csv&amp;quot;.</gco:CharacterString>
                </gmd:description>
                <gmd:source>
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                          <gco:CharacterString>Specified by BCO-DMO Data Managers</gco:CharacterString>
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				    <gco:CharacterString>Unavailable</gco:CharacterString>
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				    <gco:CharacterString>Woods Hole</gco:CharacterString>
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		  <gmd:contactInstructions>
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Description obtained from: http://web.whoi.edu/coastal-group/about/how-we-work/field-methods/coring/</gco:CharacterString>
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    <gmi:citation>
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         <gmx:Anchor xlink:href="https://vocab.ices.dk/?codeguid=9acc8274-cb6f-406c-8707-ec5a0516074a" xlink:actuate="onRequest">Community Standard Description</gmx:Anchor>
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            <gmd:date gco:nilReason="unknown"/>
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        </gmd:authority>
        <gmd:code>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/923060.rdf"
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        </gmd:code>
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    </gmi:identifier>
    <gmi:description>
      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/923060.rdf" xlink:title="R/V Falkor (too)" xlink:actuate="onRequest">vessel</gmx:Anchor>
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    <gmi:instrument gco:nilReason="unknown"/>
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                          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/person/565799.rdf" xlink:actuate="onRequest">Beth N. Orcutt</gmx:Anchor>
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