http://lod.bco-dmo.org/id/dataset/1002498
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2026-07-13
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Incubation timepoint measurements from sediment cores collected at the Southern Hydrate Ridge and Astoria Canyon on R/V Atlantis cruises AT50-14 and AT50-29B in 2023 and 2024
2026-08-19
publication
2026-08-19
revision
Woods Hole Oceanographic Institution (WHOI BCO-DMO)
2026-09-02
publication
https://doi.org/10.26008/1912/bco-dmo.1002498.1
Laura L. Lapham
University of Maryland Center for Environmental Science
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Hildebrand, A., Lapham, L. L. (2026) Incubation timepoint measurements from sediment cores collected at the Southern Hydrate Ridge and Astoria Canyon on R/V Atlantis cruises AT50-14 and AT50-29B in 2023 and 2024. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-08-19 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.1002498.1 [access date]
Incubation experiment data from Southern Hydrate Ridge and Astoria Canyon Dataset Description: Methods and Sampling: <p><strong>Core collection:</strong><br />
AT50-14: Gravity core material was sectioned with a tubing cutter, capped, and stored at 4 degrees Celsius until incubation processing. Sediment depths 50-75 centimeters (cm), 110-125 cm, and 220-230 cm were selected for incubations based on sulfate zonation.</p>
<p>AT50-29b: Push core material was sectioned through extrusion out the top at 5 cm resolution. Sediment depths 0-5 cm, 5-10 cm, and 10-15 cm were stored in three separate glass jars (Ball) and pooled with those depth intervals from two other cores at 4 degrees Celsius until incubation processing.</p>
<p><strong>Incubation setup:</strong> Headspace-free bag incubations were conducted with sediment from each study site. The overall idea was to fill foil bags with a sediment slurry that had been equilibrated with differing concentrations of CH4 and SO4 to mimic in situ geochemical conditions characteristic of each site. Three geochemical zones were targeted for each incubation to reflect the vertical zonation observed in the in situ data: the sulfate zone (SZ; high SO4, low CH4), the transition zone (TZ; low SO4, low CH4), and the methanogenic zone (low SO4, high CH4). First, in situ SO4 data was used (AC) to inform the zonation of the collected cores prior to incubation. However, SO4 data was not available for SHR when sectioning; therefore, sediments were sectioned into 5 cm intervals based on SO4 data previously reported for SHR bacterial mat sites (Boetius et al., 2000; Boetius &amp;&nbsp;Suess, 2004; Torres et al., 2002). Next, incubations were set up with experimental and control treatments. For all incubations, a slurry was created in a 2:1 sediment to anoxic MQ ratio. Slurries were sieved with a mesh strainer (steel single weave #20 mesh) to remove shell fragments that could puncture the incubation bags or clog the stopcock fitted to the incubation bag. Prior to starting incubations, storage tests were conducted to ensure the bags were gas-tight and didn’t cause isotopic fractionation. The slurries were then transferred to 500 milliliter (mL) syringes without a headspace. All slurry manipulations were conducted in an anaerobic chamber (Coy). A mixture of 100% CH4, 99% 13C-CH4 (Cambridge Isotope Laboratories), and ultra-high purity (UHP) nitrogen gas (N2) was introduced to the syringe and equilibrated with the slurry by rotating for 90 minutes to achieve CH4 concentrations targeted to in situ concentrations. After equilibration, the headspace was removed and the slurry was injected into a 1 liter (L) N2-flushed foil sampling bag equipped with a 3-way stopcock (Calibrated Instruments).</p>
<p><strong>Sediment/porewater sampling:</strong><br />
CH4, CO2, δ13C-CH4, δ13C-CO2: 10 mL glass serum vials containing 3 mL sediment slurry were subsampled using the headspace equilibration method following Magen et al. (2014). Briefly, 8 mL of ultra-zero purity air (UZA) was injected into the sediment vial with a plastic syringe equipped with a 22-gauge needle. Vials were shaken and the headspace was mixed for 2 minutes, after which 8 mL of headspace gas sample was removed. Headspace samples were subsequently diluted with UZA to fall within the instrumental range for CH4 and CO2 measurements. Because the sulfide in the incubation subsamples was not bound like in the characterization cores, a copper trap was used at the intake of the CRDS to remove sulfide before entering the machine (Malowany et al., 2015).</p>
<p>SO4 and Cl: 2 mL microcentrifuge tubes containing 40 microliters (uL) porewater sample acidified to pH 2 with 0.1M H3PO4 were diluted (135x) with Milli-Q water prior to analysis. IAPSO certified seawater standards (Ocean Scientific International Ltd.) were used for all samples and precision is +/- 2%.</p>
<p>DOC and δ13C-DOC: 40 mL amber VOA vials containing 1 mL porewater sample acidified to pH 2 with trace metal clean HCl were measured with the wet oxidation method, similarly to the setup detailed in Lalonde et al. (2014). Samples are acidified and sparged to remove inorganic carbon then reacted with Sodium persulfate as wet oxidation. Data were normalized using two different internal organic standards (precision +/- 0.5 ppm for concentrations and 0.2‰ for isotopes).</p>
<p>DIC and δ13C-DIC: 10 mL glass serum vials containing 1 mL porewater sample acidified to pH 2 with H3PO4 were measured at CBL CRDS was used as detailed for the CH4 and CO2 concentrations and stable carbon isotopes.</p>
<p>POC and δ13C-POC: After frozen sediment samples were sampled for CH4 and CO2 concentrations and stable carbon isotope ratios, one of the duplicate samples was uncapped, dried at 60 degrees Celsius (°C) for three days, ground, and acidified with vapor acidification.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2049517 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2049517
completed
Laura L. Lapham
University of Maryland Center for Environmental Science
PO Box 38
Solomons
MD
20688-0038
USA
lapham@umces.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Region
Cruise
Latitude_ddeg
Longitude_ddeg
Sampling_Date
Incubation
Classification
Zone
Treatment
Temperature
Sample_name
Days_elapsed
Bag_porewater_mL
iCH4_permille
iCO2_permille
CH4_mM
CO2_mM
SO4_mM
Cl_mM
iDIC_permille
DIC_mM
iDOC_permille
DOC_mM
percent_Corg
iPOC_permille
Conflo 4
Costech ECS 4010 elemental analyzer
gravity corer
Thermo Scientific Aquion ion chromatograph
Thermo Finnigan DeltaPlus XP IRMS
AS40 Autosampler
model 1088 autosampler
Picarro G2201-i CRDS
Push corer
Delta V Plus (Thermo Scientific)
OI Analytical model 103 0wet TIC-TOC analyzer
theme
None, User defined
region
Cruise identifier
latitude
longitude
date
experiment id
sample description
treatment
incubation temperature
sample identification
time_elapsed
volume
d13C
methane
carbon dioxide
sulfate
chloride
13C to 12C ratio in DIC
dissolved inorganic Carbon
dissolved organic Carbon
organic carbon
featureType
BCO-DMO Standard Parameters
Continuous Flow Interface for Mass Spectrometers
Costech International Elemental Combustion System (ECS) 4010
Gravity Corer
Ion Chromatograph
Isotope-ratio Mass Spectrometer
Laboratory Autosampler
Laboratory Autosampler
Picarro G2201-i isotope analyzer
Push Corer
Thermo Fisher Scientific DELTA V Plus isotope ratio mass spectrometer
Total Organic Carbon Analyzer
instrument
BCO-DMO Standard Instruments
AT50-14
AT50-29
service
Deployment Activity
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Investigating the source and flux of dissolved organic carbon released from methane seeps to the deep-ocean
https://sites.google.com/umces.edu/thelaphamlab/home/projects/seepdoc
Collaborative Research: Investigating the source and flux of dissolved organic carbon released from methane seeps to the deep-ocean
<p><em>NSF Award Abstract:</em><br />
Dissolved organic carbon (DOC) is a key component of the ocean’s food web and carbon cycle, and carbon exchanged between oceanic DOC and the atmosphere has influenced atmospheric CO2 levels on timescales ranging from recent decades to the geologic past. Production by marine algae in the surface ocean is the largest source of DOC and its effects on the ocean carbon cycle are widely appreciated. However, the contribution of DOC from additional sources such as rivers, hydrothermal vents, and methane seeps and their impact on ocean ecology and chemistry are not well understood. Each source differs in terms of its biological utilization and age, which affects the storage and distribution of DOC among the ocean basins. Methane seeps located along continental margins are particularly significant because they may transfer globally significant quantities of carbon stored below the seafloor as natural gas and gas hydrate to the oceans. This project will investigate the production, flux, composition and potential for biological utilization of DOC at Hydrate Ridge, located offshore Oregon. Hydrate Ridge is a prominent methane seep with massive accumulations of gas hydrate and a node of the Ocean Observatories Initiative telecommunications cabled array on the Juan De Fuca tectonic plate, which provides a continuous stream of real-time regional oceanographic data. We will sample and chemically characterize methane, DOC, and other materials to provide information about where the materials originated (deep vs shallow), how they have been chemically altered, to what extent they may feed deep ocean organisms, or contribute to the long term storage of DOC in the ocean. Experiments and analysis will be conducted using sediment cores and bottom water samples collected using either the remotely operated vehicle Jason or the human occupied vehicle Alvin during a 7-day ocean expedition. Additionally, this project will place osmotically-driven pumps on the seafloor to continuously sample fluids for approximately one year, thereby allowing us to monitor the movement of methane and DOC expelled from the seafloor to the ocean and constrain processes that regulate the release of carbon to the oceans at methane seeps. This project will support one graduate student and several undergraduates from a community college in Maryland and a college located in a lower-income urban center in southeastern Massachusetts. We will disseminate project findings to the public with a series of videos for public TV.</p>
<p>This study will investigate the production, flux and reactivity of methane-derived dissolved organic carbon (DOC) from methane (CH4) seeps at Hydrate Ridge, Offshore Oregon. The study will address four fundamental questions to determine the significance of CH4-derived DOC within the ocean carbon cycle: (1) How much CH4-derived fossil DOC do seeps contribute to the oceans? (2) To what extent is CH4-derived C incorporated into DOC during anaerobic oxidation of CH4? (3) Is seep DOC bioavailable or recalcitrant when released into the deep ocean? (4) How does the flux of DOC to the water column vary over time? We will employ an interdisciplinary strategy that includes in situ sampling, laboratory incubations, and a comprehensive analytical geochemistry program. Data from the Ocean Observatories Initiative Regional Cabled Array at Southern Hydrate Ridge will be used to provide context for field and experimental data. The composition and abundance of organic and inorganic chemical species along with the stable and radiocarbon isotope composition of pore water, bulk sediment, and water column C pools will be used to identify DOC sources and quantify fluxes from cold seeps characterized by a range of advection rates. The centerpiece of the investigation will be a 7-day research cruise to Hydrate Ridge to collect sediments, pore fluids, and water column samples, and deploy OsmoSamplers for continuous time series fluid sampling. The results will form the foundation for estimating the contribution of CH4-derived DOC to the oceanic DOC pool.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
seepDOM
largerWorkCitation
project
eng; USA
oceans
-125.147051
-124.6026513
44.569866
46.240541
2023-08-18
2024-08-21
Astoria Canyon and Hydrate Ridge
0
BCO-DMO catalogue of parameters from Incubation timepoint measurements from sediment cores collected at the Southern Hydrate Ridge and Astoria Canyon on R/V Atlantis cruises AT50-14 and AT50-29B in 2023 and 2024
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/1005713.rdf
Name: Region
Units: unitless
Description: <p>Geographic region</p>
http://lod.bco-dmo.org/id/dataset-parameter/1005714.rdf
Name: Cruise
Units: unitless
Description: <p>Cruise ID for sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/1005715.rdf
Name: Latitude_ddeg
Units: decimal degrees
Description: <p>Sample latitude, south is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/1005717.rdf
Name: Longitude_ddeg
Units: decimal degrees
Description: <p>Sample longitude, west is negative</p>
http://lod.bco-dmo.org/id/dataset-parameter/1005719.rdf
Name: Sampling_Date
Units: unitless
Description: <p>Date the sample was collected</p>
http://lod.bco-dmo.org/id/dataset-parameter/1005720.rdf
Name: Incubation
Units: unitless
Description: <p>Incubation ID</p>
http://lod.bco-dmo.org/id/dataset-parameter/1005721.rdf
Name: Classification
Units: unitless
Description: <p>For Incubation SHR, samples were either taken from a microbial mat, 'Mat', or a reference site, 'Ref'</p>
http://lod.bco-dmo.org/id/dataset-parameter/1005722.rdf
Name: Zone
Units: unitless
Description: <p>Zone the incubation samples were taken from. SZ = sulfate zone; TZ = transition zone; MZ = methanogenic zone</p>
http://lod.bco-dmo.org/id/dataset-parameter/1005723.rdf
Name: Treatment
Units: unitless
Description: <p>Treatment applied to the incubation vessel. 13CH4 = 13C-labeled methane added; 13CH4+base = 13C-labeled methane and base added; 13CH4 + BES = 13C-labeled methane and 2-bromoethanesulfonate added; 12CH4 = non-13C-labeled methane added</p>
http://lod.bco-dmo.org/id/dataset-parameter/1005724.rdf
Name: Temperature
Units: degrees Celsius (°C)
Description: <p>Temperature the incubation bags were stored at for the duration of the experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/1005725.rdf
Name: Sample_name
Units: unitless
Description: <p>The combined zone + treatment + temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/1005726.rdf
Name: Days_elapsed
Units: days
Description: <p>Time since the incubation was started, defined as the day since the incubation vessels were filled</p>
http://lod.bco-dmo.org/id/dataset-parameter/1005727.rdf
Name: Bag_porewater_mL
Units: milliliters (mL)
Description: <p>Volume of bag porewater after samples were taken</p>
http://lod.bco-dmo.org/id/dataset-parameter/1005728.rdf
Name: iCH4_permille
Units: per mille (‰)
Description: <p>Bulk stable carbon isotope value of pore water dissolved methane relative to Vienna Pee Dee Belemnite (VPDB)</p>
http://lod.bco-dmo.org/id/dataset-parameter/1005729.rdf
Name: iCO2_permille
Units: per mille (‰)
Description: <p>Bulk stable carbon isotope value of pore water dissolved carbon dioxide relative to Vienna Pee Dee Belemnite (VPDB)</p>
http://lod.bco-dmo.org/id/dataset-parameter/1005730.rdf
Name: CH4_mM
Units: millimolar (mM)
Description: <p>Pore water dissolved methane</p>
http://lod.bco-dmo.org/id/dataset-parameter/1005731.rdf
Name: CO2_mM
Units: millimolar (mM)
Description: <p>Pore water dissolved carbon dioxide</p>
http://lod.bco-dmo.org/id/dataset-parameter/1005732.rdf
Name: SO4_mM
Units: millimolar (mM)
Description: <p>Pore water dissolved sulfate</p>
http://lod.bco-dmo.org/id/dataset-parameter/1005733.rdf
Name: Cl_mM
Units: millimolar (mM)
Description: <p>Pore water dissolved chloride</p>
http://lod.bco-dmo.org/id/dataset-parameter/1005734.rdf
Name: iDIC_permille
Units: per mille (‰)
Description: <p>Bulk stable carbon isotope value of pore water dissolved inorganic carbon relative to Vienna Pee Dee Belemnite (VPDB)</p>
http://lod.bco-dmo.org/id/dataset-parameter/1005735.rdf
Name: DIC_mM
Units: millimolar (mM)
Description: <p>Pore water dissolved inorganic carbon</p>
http://lod.bco-dmo.org/id/dataset-parameter/1005736.rdf
Name: iDOC_permille
Units: per mille (‰)
Description: <p>Bulk stable carbon isotope value of pore water dissolved organic carbon relative to Vienna Pee Dee Belemnite (VPDB)</p>
http://lod.bco-dmo.org/id/dataset-parameter/1005737.rdf
Name: DOC_mM
Units: millimolar (mM)
Description: <p>Pore water dissolved organic carbon</p>
http://lod.bco-dmo.org/id/dataset-parameter/1005738.rdf
Name: percent_Corg
Units: percent (%)
Description: <p>Sediment percent organic matter</p>
http://lod.bco-dmo.org/id/dataset-parameter/1005739.rdf
Name: iPOC_permille
Units: per mille (‰)
Description: <p>Bulk stable carbon isotope value of pore water particulate organic carbon relative to Vienna Pee Dee Belemnite (VPDB)</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://doi.org/10.26008/1912/bco-dmo.1002498.1
download
onLine
dataset
<p><strong>Core collection:</strong><br />
AT50-14: Gravity core material was sectioned with a tubing cutter, capped, and stored at 4 degrees Celsius until incubation processing. Sediment depths 50-75 centimeters (cm), 110-125 cm, and 220-230 cm were selected for incubations based on sulfate zonation.</p>
<p>AT50-29b: Push core material was sectioned through extrusion out the top at 5 cm resolution. Sediment depths 0-5 cm, 5-10 cm, and 10-15 cm were stored in three separate glass jars (Ball) and pooled with those depth intervals from two other cores at 4 degrees Celsius until incubation processing.</p>
<p><strong>Incubation setup:</strong> Headspace-free bag incubations were conducted with sediment from each study site. The overall idea was to fill foil bags with a sediment slurry that had been equilibrated with differing concentrations of CH4 and SO4 to mimic in situ geochemical conditions characteristic of each site. Three geochemical zones were targeted for each incubation to reflect the vertical zonation observed in the in situ data: the sulfate zone (SZ; high SO4, low CH4), the transition zone (TZ; low SO4, low CH4), and the methanogenic zone (low SO4, high CH4). First, in situ SO4 data was used (AC) to inform the zonation of the collected cores prior to incubation. However, SO4 data was not available for SHR when sectioning; therefore, sediments were sectioned into 5 cm intervals based on SO4 data previously reported for SHR bacterial mat sites (Boetius et al., 2000; Boetius &amp;&nbsp;Suess, 2004; Torres et al., 2002). Next, incubations were set up with experimental and control treatments. For all incubations, a slurry was created in a 2:1 sediment to anoxic MQ ratio. Slurries were sieved with a mesh strainer (steel single weave #20 mesh) to remove shell fragments that could puncture the incubation bags or clog the stopcock fitted to the incubation bag. Prior to starting incubations, storage tests were conducted to ensure the bags were gas-tight and didn’t cause isotopic fractionation. The slurries were then transferred to 500 milliliter (mL) syringes without a headspace. All slurry manipulations were conducted in an anaerobic chamber (Coy). A mixture of 100% CH4, 99% 13C-CH4 (Cambridge Isotope Laboratories), and ultra-high purity (UHP) nitrogen gas (N2) was introduced to the syringe and equilibrated with the slurry by rotating for 90 minutes to achieve CH4 concentrations targeted to in situ concentrations. After equilibration, the headspace was removed and the slurry was injected into a 1 liter (L) N2-flushed foil sampling bag equipped with a 3-way stopcock (Calibrated Instruments).</p>
<p><strong>Sediment/porewater sampling:</strong><br />
CH4, CO2, δ13C-CH4, δ13C-CO2: 10 mL glass serum vials containing 3 mL sediment slurry were subsampled using the headspace equilibration method following Magen et al. (2014). Briefly, 8 mL of ultra-zero purity air (UZA) was injected into the sediment vial with a plastic syringe equipped with a 22-gauge needle. Vials were shaken and the headspace was mixed for 2 minutes, after which 8 mL of headspace gas sample was removed. Headspace samples were subsequently diluted with UZA to fall within the instrumental range for CH4 and CO2 measurements. Because the sulfide in the incubation subsamples was not bound like in the characterization cores, a copper trap was used at the intake of the CRDS to remove sulfide before entering the machine (Malowany et al., 2015).</p>
<p>SO4 and Cl: 2 mL microcentrifuge tubes containing 40 microliters (uL) porewater sample acidified to pH 2 with 0.1M H3PO4 were diluted (135x) with Milli-Q water prior to analysis. IAPSO certified seawater standards (Ocean Scientific International Ltd.) were used for all samples and precision is +/- 2%.</p>
<p>DOC and δ13C-DOC: 40 mL amber VOA vials containing 1 mL porewater sample acidified to pH 2 with trace metal clean HCl were measured with the wet oxidation method, similarly to the setup detailed in Lalonde et al. (2014). Samples are acidified and sparged to remove inorganic carbon then reacted with Sodium persulfate as wet oxidation. Data were normalized using two different internal organic standards (precision +/- 0.5 ppm for concentrations and 0.2‰ for isotopes).</p>
<p>DIC and δ13C-DIC: 10 mL glass serum vials containing 1 mL porewater sample acidified to pH 2 with H3PO4 were measured at CBL CRDS was used as detailed for the CH4 and CO2 concentrations and stable carbon isotopes.</p>
<p>POC and δ13C-POC: After frozen sediment samples were sampled for CH4 and CO2 concentrations and stable carbon isotope ratios, one of the duplicate samples was uncapped, dried at 60 degrees Celsius (°C) for three days, ground, and acidified with vapor acidification.</p>
Specified by the Principal Investigator(s)
<p>Methane, carbon dioxide, and dissolved inorganic carbon concentrations and stable carbon isotope ratios: Software for Picarro was used to obtain the CH4, CO2, and DIC concentrations (ppm) as well as stable carbon isotope ratios (delta notation; per mille). Concentrations were converted to dissolved concentrations (mM) and stable carbon isotope ratios were corrected for the machine offset using Microsoft Excel.</p>
<p>Sulfate and chloride: Chromeleon 7 software was used to obtain areas for SO4 and Cl. Areas were converted to concentrations (mM) using Microsoft Excel.</p>
<p>Particulate organic carbon concentrations and stable carbon isotope ratios: EAS Claity 3.0.0.154 and Isodat Acquisition Version 3.0 were used to obtain POC concentrations (%) and stable carbon isotope ratios (delta notation; per mille). Concentrations and stable carbon isotope ratios were corrected for machine drift using Microsoft Excel.</p>
Specified by the Principal Investigator(s)
- Imported sheet 1 of the original file "BCO_DMO_AC_SHR_v2.xlsx" into the BCO-DMO data processing system.
- Treated "#DIV/0!" and "#VALUE!" as missing values (note that missing values are empty/blank in the final CSV file).
- Converted "Sampling_Date" column from string format %m-%d-%y to date type with output format %Y-%m-%d.
- Saved the final file as "1002498_v1_inc_exp_ac_shr.csv".
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Conflo 4
Conflo 4
PI Supplied Instrument Name: Conflo 4 PI Supplied Instrument Description:Dissolved organic carbon concentration and stable carbon isotopes were measured with an OI Analytical model 1030 wet TIC-TOC analyzer with a model 1088 autosampler interfaced to a Thermo Finnigan DeltaPlus XP IRMS via a Conflo 4 at Jan Veizer Stable Isotope Laboratory. Instrument Name: Continuous Flow Interface for Mass Spectrometers Instrument Short Name: Instrument Description: A Continuous Flow Interface connects solid and liquid sample preparation devices to instruments that measure isotopic composition. It allows the introduction of the sample and also reference and carrier gases.
Examples: Finnigan MATConFlo II, ThermoScientific ConFlo IV, and Picarro Caddy.
Note: This is NOT an analyzer
Costech ECS 4010 elemental analyzer
Costech ECS 4010 elemental analyzer
PI Supplied Instrument Name: Costech ECS 4010 elemental analyzer PI Supplied Instrument Description:Particulate organic carbon and stable carbon isotopes were measured with a Costech ECS 4010 elemental analyzer equipped with a thermal conduction detector and connected to a continuous flow isotope ratio mass spectrometer Delta V Plus (Thermo Scientific) using He as the carrier gas at Chesapeake Biological Laboratory. Instrument Name: Costech International Elemental Combustion System (ECS) 4010 Instrument Short Name:Costech ECS 4010 Instrument Description: The ECS 4010 Nitrogen / Protein Analyzer is an elemental combustion analyser for CHNSO elemental analysis and Nitrogen / Protein determination. The GC oven and separation column have a temperature range of 30-110 degC, with control of +/- 0.1 degC.
gravity corer
gravity corer
PI Supplied Instrument Name: gravity corer PI Supplied Instrument Description:On AT50-14, gravity core material was sectioned with a tubing cutter, capped, and stored until incubation processing. Instrument Name: Gravity Corer Instrument Short Name:Gravity Corer Instrument Description: The gravity corer allows researchers to sample sediment layers at the bottom of lakes or oceans. The coring device is deployed from the ship and gravity carries it to the seafloor. From: http://www.whoi.edu/instruments/viewInstrument.do?id=1079 Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/51/
Thermo Scientific Aquion ion chromatograph
Thermo Scientific Aquion ion chromatograph
PI Supplied Instrument Name: Thermo Scientific Aquion ion chromatograph PI Supplied Instrument Description:Sulfate and chloride were measured with a Thermo Scientific Aquion ion chromatograph (IonPac AG22 4x50 mm guard column, IonPac AS22 4x250 mm analytical column, and AERS 300 4 mm suppressor) with an AS40 Autosampler at Chesapeake Biological Laboratory. Instrument Name: Ion Chromatograph Instrument Short Name:Ion Chromatograph Instrument Description: Ion chromatography is a form of liquid chromatography that measures concentrations of ionic species by separating them based on their interaction with a resin. Ionic species separate differently depending on species type and size. Ion chromatographs are able to measure concentrations of major anions, such as fluoride, chloride, nitrate, nitrite, and sulfate, as well as major cations such as lithium, sodium, ammonium, potassium, calcium, and magnesium in the parts-per-billion (ppb) range. From: http://serc.carleton.edu/microbelife/research_methods/biogeochemical/ic.html
Thermo Finnigan DeltaPlus XP IRMS
Thermo Finnigan DeltaPlus XP IRMS
PI Supplied Instrument Name: Thermo Finnigan DeltaPlus XP IRMS PI Supplied Instrument Description:Dissolved organic carbon concentration and stable carbon isotopes were measured with an OI Analytical model 1030 wet TIC-TOC analyzer with a model 1088 autosampler interfaced to a Thermo Finnigan DeltaPlus XP IRMS via a Conflo 4 at Jan Veizer Stable Isotope Laboratory. Instrument Name: Isotope-ratio Mass Spectrometer Instrument Short Name:IR Mass Spec; IRMS Instrument Description: The Isotope-ratio Mass Spectrometer is a particular type of mass spectrometer used to measure the relative abundance of isotopes in a given sample (e.g. VG Prism II Isotope Ratio Mass-Spectrometer). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
AS40 Autosampler
AS40 Autosampler
PI Supplied Instrument Name: AS40 Autosampler PI Supplied Instrument Description:Sulfate and chloride were measured with a Thermo Scientific Aquion ion chromatograph (IonPac AG22 4x50 mm guard column, IonPac AS22 4x250 mm analytical column, and AERS 300 4 mm suppressor) with an AS40 Autosampler at Chesapeake Biological Laboratory. Instrument Name: Laboratory Autosampler Instrument Short Name: Instrument Description: Laboratory apparatus that automatically introduces one or more samples with a predetermined volume or mass into an analytical instrument.
model 1088 autosampler
model 1088 autosampler
PI Supplied Instrument Name: model 1088 autosampler PI Supplied Instrument Description:Dissolved organic carbon concentration and stable carbon isotopes were measured with an OI Analytical model 1030 wet TIC-TOC analyzer with a model 1088 autosampler interfaced to a Thermo Finnigan DeltaPlus XP IRMS via a Conflo 4 at Jan Veizer Stable Isotope Laboratory. Instrument Name: Laboratory Autosampler Instrument Short Name: Instrument Description: Laboratory apparatus that automatically introduces one or more samples with a predetermined volume or mass into an analytical instrument.
Picarro G2201-i CRDS
Picarro G2201-i CRDS
PI Supplied Instrument Name: Picarro G2201-i CRDS PI Supplied Instrument Description:Dissolved methane, carbon dioxide, and dissolved inorganic carbon concentrations and stable carbon isotopes were determined using a Picarro G2201-i CRDS at Chesapeake Biological Laboratory. Instrument Name: Picarro G2201-i isotope analyzer Instrument Short Name: Instrument Description: The G2201-i Isotopic Analyzer measures d13C for CH4 and CO2. See: https://www.picarro.com/products/g2201i_isotopic_analyzer
Push corer
Push corer
PI Supplied Instrument Name: Push corer PI Supplied Instrument Description:On AT50-29, push core material was sectioned through extrusion out the top at 5 cm resolution. Instrument Name: Push Corer Instrument Short Name: Instrument Description: Capable of being performed in numerous environments, push coring is just as it sounds. Push coring is simply pushing the core barrel (often an aluminum or polycarbonate tube) into the sediment by hand. A push core is useful in that it causes very little disturbance to the more delicate upper layers of a sub-aqueous sediment.
Description obtained from: http://web.whoi.edu/coastal-group/about/how-we-work/field-methods/coring/
Delta V Plus (Thermo Scientific)
Delta V Plus (Thermo Scientific)
PI Supplied Instrument Name: Delta V Plus (Thermo Scientific) PI Supplied Instrument Description:Particulate organic carbon and stable carbon isotopes were measured with a Costech ECS 4010 elemental analyzer equipped with a thermal conduction detector and connected to a continuous flow isotope ratio mass spectrometer Delta V Plus (Thermo Scientific) using He as the carrier gas at Chesapeake Biological Laboratory. Instrument Name: Thermo Fisher Scientific DELTA V Plus isotope ratio mass spectrometer Instrument Short Name:Delta V Plus IRMS Instrument Description: The Thermo Scientific DELTA V Plus is an isotope ratio mass spectrometer designed to measure isotopic, elemental and molecular ratios of organic and inorganic compounds. The DELTA V Plus is an enhanced model of the DELTA V series of isotope ratio mass spectrometers, which can be upgraded from the DELTA V Advantage. The DELTA V Plus can be operated in Continuous Flow or Dual Inlet mode and can accommodate up to 10 collectors, ensuring flexibility to cover many applications. The DELTA V Plus is controlled by an automated, integrated Isodat software suite. A magnet, whose pole faces determine the free flight space for the ions, eliminates the traditional flight tube. The magnet is designed for fast mass switching which is further supported by a fast jump control between consecutive measurements of multiple gases within one run. The sample gas is introduced at ground potential, eliminating the need for insulation of the flow path, ensuring 100 percent transfer into the ion source. The amplifiers register ion beams up to 50 V. The DELTA V Plus has refined optics, enabling greater ion transmission than the DELTA V Advantage. It has a sensitivity of 800 molecules per ion (M/I) in Dual Inlet mode and 1100 M/I in Continuous Flow mode. It has a system stability of < 10 ppm and an effective magnetic detection radius of 191 nm. It has a mass range of 1 - 96 Dalton at 3 kV.
OI Analytical model 103 0wet TIC-TOC analyzer
OI Analytical model 103 0wet TIC-TOC analyzer
PI Supplied Instrument Name: OI Analytical model 103 0wet TIC-TOC analyzer PI Supplied Instrument Description:Dissolved organic carbon concentration and stable carbon isotopes were measured with an OI Analytical model 1030 wet TIC-TOC analyzer with a model 1088 autosampler interfaced to a Thermo Finnigan DeltaPlus XP IRMS via a Conflo 4 at Jan Veizer Stable Isotope Laboratory. Instrument Name: Total Organic Carbon Analyzer Instrument Short Name:TOC analyzer Instrument Description: A unit that accurately determines the carbon concentrations of organic compounds typically by detecting and measuring its combustion product (CO2). See description document at: http://bcodata.whoi.edu/LaurentianGreatLakes_Chemistry/bs116.pdf Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/
Cruise: AT50-14
AT50-14
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
AT50-14
Laura L. Lapham
University of Maryland Center for Environmental Science
Cruise: AT50-29
AT50-29
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
AT50-29
Michael Vardaro
University of Washington
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel