http://lod.bco-dmo.org/id/dataset/1003038
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2026-07-27
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Size fractionated organic carbon and 13C at Bermuda Atlantic Time Series (BATS) from samples collected on R/V Atlantic Explorer cruise AE1920 in August 2019
2026-07-27
publication
2026-07-27
revision
BCO-DMO Linked Data URI
2026-07-27
creation
http://lod.bco-dmo.org/id/dataset/1003038
Jacob A. Cram
University of Maryland Center for Environmental Science
principalInvestigator
Clara Fuchsman
University of Maryland Center for Environmental Science
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Fuchsman, C., Cram, J. A. (2026) Size fractionated organic carbon and 13C at Bermuda Atlantic Time Series (BATS) from samples collected on R/V Atlantic Explorer cruise AE1920 in August 2019. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-07-27 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/1003038 [access date]
BATS 2019 Size Fractionated Organic C and 13C Dataset Description: Methods and Sampling: <p>For size fractionated suspended particulate organic matter in August 2019, samples were obtained from Niskin bottles but were collected by opening the bottom of the Niskin bottle into an acid cleaned bucket. This ensured that particles that had sunk below the spigot of the Niskin bottle were included. 120 liters (L) of water was gravity filtered, in sequence, through nylon mesh (142-millimeter (mm) diameter) of decreasing pore size (500, 180, 53, 20 micrometers (µm)) and a subset of this 20 µm filtered water (~20 L) was then filtered through a 5 µm mesh. For mesh sizes 20 µm and above, the large diameter of the mesh and abundance of functional pore-space prevented clogging, and water flowed through the mesh quickly, indicating that clogging did not occur. Water filtered more slowly through the 5 µm mesh (on the order of 30 minutes). After filtration, each nylon mesh was back-rinsed with ~500 milliliters (mL) of prefiltered 60 kd filtered seawater to produce a resuspension of particulate matter from particles from each size class. The 60 kd filtered seawater had been produced by tangential flow filtration as a byproduct of virus isolation experiments on the same cruise. After back-rinsing, the resuspended particles were split with one half used for particulate matter measurements. In all cases, the actual volumes were carefully recorded and used for normalization during analysis. The resuspended particulate matter from each sample and size class was collected by vacuum filtration through a 1.2 µm nominal pore size, 25 mm diameter, GF/C glass fiber filter (Whatman WHA1822025). These filters had been previously pre-combusted for at least two hours at 400 degrees Celsius (°C). Two depths were sampled per cast, and multiple casts were combined to represent the station. Samples were immediately frozen.</p>
<p>At Horn Point, samples were dried at 40°C and stored in a desiccator. For processing, they were wafted with HCl overnight to remove carbonate, dried at 40°C, packed in both silver and tin capsules, and sent to the UC Davis Stable Isotope Facility for C and N analysis utilizing an Elemental Analyzer (Elementar Vario EL Cube) attached to an Isotope Ratio Mass Spectrometer (Isoprime VisION). Blank combusted GF/C filters were included in analyses and did not show measurable material.</p>
<p>For the 90-meter (m) sample, the entire Niskin rosette was fired at the same depth. Particles were fractionated for both size and sinking. In this case, the CTD sat on deck for one hour while the particles were allowed to settle. After one hour, the top ⅔ of each Niskin bottle were siphoned off and saved. Then the bottom ⅓ of each bottle was collected by opening the bottom of the Niskin bottle into an acid-cleaned bucket. The upper and lower fractions were then filtered using the size fractionation method described above.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1829888 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1829888
onGoing
Jacob A. Cram
University of Maryland Center for Environmental Science
2020 Horns Point Rd
Cambridge
MD
USA
jcram@umces.edu
pointOfContact
Clara Fuchsman
University of Maryland Center for Environmental Science
P. O. Box 775 2020 Horns Point Rd
Cambridge
MD
21613
USA
cfuchsman@umces.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Station
latitude
longitude
Date
Depth
Section
Size_Fraction
d13C_VPDB
Carbon
Isoprime VisION
Elementar Vario EL Cube
Niskin bottles
theme
None, User defined
station
latitude
longitude
date
depth
sample description
sample fraction (includes size fractions)
d13C
particulate organic Carbon (POC)
featureType
BCO-DMO Standard Parameters
Elementar Isoprime Vision (IRMS) isotopic ratio mass spectrometer
Elementar Vario EL Cube elemental analyzer
Niskin bottle
instrument
BCO-DMO Standard Instruments
AE1920
service
Deployment Activity
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
The fate of lysis products of picocyanobacteria contributes to marine humic-like chromophoric dissolved organic matter
https://osprey.bco-dmo.org/project/806071
The fate of lysis products of picocyanobacteria contributes to marine humic-like chromophoric dissolved organic matter
<p><em>NSF Award Abstract:</em><br />
This study focuses on the sources and composition of colored dissolved organic matter (CDOM) in the ocean. CDOM is a part of water that absorbs sunlight. This material is important because it filters out harmful ultraviolet radiation. Scientists use it to track the movement of carbon and other important biological and chemical processes in the ocean. Organisms such as algae living in the open ocean have been shown to be sources of CDOM, but the chemical composition of these algal natural products remains to be discovered. Recent results from studying common algae show that viruses may break down algal cells and release material that looks like CDOM. This study will use new tools to find out if viruses and algae are creating this material and study its chemical makeup. This project will support two graduate students and provide summer internships for undergraduates through the NSF Research Experiences for Undergraduates (REU) program. The investigators will participate in a range of education and outreach activities.</p>
<p>The sources and structural nature of marine CDOM within the oceans remain unclear and continue to be a subject of debate. Marine in situ sources of CDOM have been suggested and some have been confirmed, but thus far none could explain the ubiquitous appearance of the so called "humic-like" CDOM component. Unique features of this component include its unusual exponential behavior in ultraviolet-visible (UV-Vis) absorbance with the absorbance extending well above 400 nm, and the large Stoke's shift in fluorescence spectroscopy. Picocyanobacteria are ubiquitous in the World's Oceans and make up 50 % of the autotrophic marine primary production. Preliminary results showed that the picocyanobacteria Synechococcus and Prochlorococcus release CDOM that matched the "humic-like" appearance of globally observed marine CDOM after virus-induced lysis. The main focus of this study is the characterization of the optical properties and molecular composition of viral-lysed DOM (VDOM) from different strains of Synechococcus and Prochlorococcus and additionally Trichodesmium which was shown in a previous study to also release CDOM. Associations between the chemical characterization information and metagenomics and transcriptomics data will be investigated for picocyanobacteria in the Pacific and Atlantic Oceans. This study includes long-term incubation experiments to determine the persistence of picocyanobacteria-derived CDOM as well as changes in microbial communities and processes (gene expression) that are related to the degradation of VDOM during the incubation period.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Picocyanobacteria CDOM
largerWorkCitation
project
eng; USA
oceans
-64.182
-64.1601
31.6665
31.6751
2019-08-08
2019-08-12
Bermuda Atlantic Time Series Station (BATS) and station Aloha, Hawaii
0
BCO-DMO catalogue of parameters from Size fractionated organic carbon and 13C at Bermuda Atlantic Time Series (BATS) from samples collected on R/V Atlantic Explorer cruise AE1920 in August 2019
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/1003051.rdf
Name: Station
Units: unitless
Description: <p>station sampled</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003052.rdf
Name: latitude
Units: decimal degrees
Description: <p>Latitude of location sampled. degrees with a cardinal direction N.</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003054.rdf
Name: longitude
Units: decimal degrees
Description: <p>Longitude of location sampled. degrees with a cardinal direction W</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003056.rdf
Name: Date
Units: unitless
Description: <p>date sampled</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003057.rdf
Name: Depth
Units: meters (m)
Description: <p>depth sampled</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003059.rdf
Name: Section
Units: unitless
Description: <p>Description of the part of the bottle sampled (e.g. 'Whole bottle', 'Bottom 1/3')</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003060.rdf
Name: Size_Fraction
Units: unitless
Description: <p>particle size range description (e.g. '>500','53-180'). Numbers in the size range description are in microns (um).</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003061.rdf
Name: d13C_VPDB
Units: permil (0/00)
Description: <p>isotopic composition of C. The stable carbon isotope ratio d13C relative to the Vienna Pee Dee Belemnite (VPDB) international standard.</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003062.rdf
Name: Carbon
Units: micromolar (uM)
Description: <p>concentration of organic C</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://osprey.bco-dmo.org/dataset/1003038/data/download
download
onLine
dataset
<p>For size fractionated suspended particulate organic matter in August 2019, samples were obtained from Niskin bottles but were collected by opening the bottom of the Niskin bottle into an acid cleaned bucket. This ensured that particles that had sunk below the spigot of the Niskin bottle were included. 120 liters (L) of water was gravity filtered, in sequence, through nylon mesh (142-millimeter (mm) diameter) of decreasing pore size (500, 180, 53, 20 micrometers (µm)) and a subset of this 20 µm filtered water (~20 L) was then filtered through a 5 µm mesh. For mesh sizes 20 µm and above, the large diameter of the mesh and abundance of functional pore-space prevented clogging, and water flowed through the mesh quickly, indicating that clogging did not occur. Water filtered more slowly through the 5 µm mesh (on the order of 30 minutes). After filtration, each nylon mesh was back-rinsed with ~500 milliliters (mL) of prefiltered 60 kd filtered seawater to produce a resuspension of particulate matter from particles from each size class. The 60 kd filtered seawater had been produced by tangential flow filtration as a byproduct of virus isolation experiments on the same cruise. After back-rinsing, the resuspended particles were split with one half used for particulate matter measurements. In all cases, the actual volumes were carefully recorded and used for normalization during analysis. The resuspended particulate matter from each sample and size class was collected by vacuum filtration through a 1.2 µm nominal pore size, 25 mm diameter, GF/C glass fiber filter (Whatman WHA1822025). These filters had been previously pre-combusted for at least two hours at 400 degrees Celsius (°C). Two depths were sampled per cast, and multiple casts were combined to represent the station. Samples were immediately frozen.</p>
<p>At Horn Point, samples were dried at 40°C and stored in a desiccator. For processing, they were wafted with HCl overnight to remove carbonate, dried at 40°C, packed in both silver and tin capsules, and sent to the UC Davis Stable Isotope Facility for C and N analysis utilizing an Elemental Analyzer (Elementar Vario EL Cube) attached to an Isotope Ratio Mass Spectrometer (Isoprime VisION). Blank combusted GF/C filters were included in analyses and did not show measurable material.</p>
<p>For the 90-meter (m) sample, the entire Niskin rosette was fired at the same depth. Particles were fractionated for both size and sinking. In this case, the CTD sat on deck for one hour while the particles were allowed to settle. After one hour, the top ⅔ of each Niskin bottle were siphoned off and saved. Then the bottom ⅓ of each bottle was collected by opening the bottom of the Niskin bottle into an acid-cleaned bucket. The upper and lower fractions were then filtered using the size fractionation method described above.</p>
Specified by the Principal Investigator(s)
<p>Micrograms (ug) of C and N were converted to concentrations in Microsoft Excel using volumes filtered.&nbsp;</p>
Specified by the Principal Investigator(s)
- Imported original file "BATS_OrgC_13C_2.xlsx" into the BCO-DMO data processing system.
- Converted the "Date" column from "%d-%b-%y" format (e.g., "15-Jan-20") to ISO date format "%Y-%m-%d" in UTC.
- Converted "latitude" column from degrees-decimal minutes format (e.g., "31°45.0'N") to decimal degrees with North directional.
- Converted "longitude" column from degrees-decimal minutes format (e.g., "64°10.0'W") to decimal degrees with West directional.
- Rounded "latitude" and "longitude" to 4 decimal places, applying only to values with more precision than 4 digits and preserving trailing zeros.
- Saved the final file as "1003038_v1_bats_orgc_13c.csv".
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Isoprime VisION
Isoprime VisION
PI Supplied Instrument Name: Isoprime VisION PI Supplied Instrument Description:At the UC Davis Stable Isotope Facility samples were analyzed utilizing an Elemental Analyzer (Elementar Vario EL Cube) attached to an Isotope Ratio Mass Spectrometer (Isoprime VisION). Instrument Name: Elementar Isoprime Vision (IRMS) isotopic ratio mass spectrometer Instrument Short Name:Isoprime Vision Instrument Description: A laboratory instrument. It is used for mesauring the ratios of stable isotopes in a variety of applications such as in honey, ecology, geology and petroleum applications. It can aid in the measurement of 2H, 13C, 15N, 18O and 34S stable isotopes. It uses a thorium coated filament, stainless steel vacuum chamber, 100 V amplification and purge and trap chromatography for the separation of gases. It is also capable of headspace analysis. It is able to perform bulk and compound specific analysis through combination with other Elementar and Agilent technologies such as gas chromatography and elemental analysis.
Elementar Vario EL Cube
Elementar Vario EL Cube
PI Supplied Instrument Name: Elementar Vario EL Cube PI Supplied Instrument Description:At the UC Davis Stable Isotope Facility samples were analyzed utilizing an Elemental Analyzer (Elementar Vario EL Cube) attached to an Isotope Ratio Mass Spectrometer (Isoprime VisION). Instrument Name: Elementar Vario EL Cube elemental analyzer Instrument Short Name:Vario EL Cube Instrument Description: A laboratory instrument used for quantifying organic elements. It can measure C, H, N and S and optionally O, Cl and TIC. It was first developed in 2006 as a successor to the vario EL III. It uses a high-temperature combustion unit that is able to complete sample digestion at up to 1200 deg C (or 1800 deg C at the point of combustion when tin foil is used) and a jet injection of oxygen directly to the sample during combustion. Separation of gas components are performed on up to 3 gas-selective columns which trap gases until they are heated up and the prior gas peak has reached the baseline during detection. It uses a Thermal Conductivity Detector (TCD) as standard. An infrared (IR) detector for sulfur and oxygen and electrochemical detector for chlorine are optionally available. The instrument can measure C / N elemental ratios of up to 12,000:1 and provides an elemental detection limit of < 40 ppm (TCD).
Niskin bottles
Niskin bottles
PI Supplied Instrument Name: Niskin bottles PI Supplied Instrument Description:Samples were obtained from Niskin bottles. Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Cruise: AE1920
AE1920
R/V Atlantic Explorer
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantic Explorer
vessel
AE1920
Michael Gonsior
University of Maryland Center for Environmental Science
R/V Atlantic Explorer
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantic Explorer
vessel