http://lod.bco-dmo.org/id/dataset/1003286
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2026-07-30
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Biogeochemical and microbial parameters collected during diel surveys on a coral reef at Mo'orea's North Shore in French Polynesia in 2021, 2022, and 2023
2026-08-03
publication
2026-08-03
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2026-08-03
publication
https://doi.org/10.26008/1912/bco-dmo.1003286.1
Craig E. Nelson
University of Hawaiʻi at Mānoa
principalInvestigator
Linda Wegley Kelly
University of California-San Diego Scripps
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Wegley Kelly, L., Nelson, C. E. (2026) Biogeochemical and microbial parameters collected during diel surveys on a coral reef at Mo'orea's North Shore in French Polynesia in 2021, 2022, and 2023. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-08-03 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.1003286.1 [access date]
Mo'orea North Shore Diel Surveys 2021-2023 Dataset Description: Methods and Sampling: <p><em>Sampling Dates:</em><br />
2021:<br />
Gump Station, Moorea, French Polynesia<br />
Aug 10 to Aug 19, 2021<br />
Chief Scientist - Linda Wegley Kelly (SIO, lwegley@ucsd.edu)</p>
<p>2022:<br />
Gump Station, Moorea, French Polynesia<br />
Mar 29 to Apr 15, 2022<br />
Chief Scientist - Linda Wegley Kelly</p>
<p>2023:<br />
Gump Station, Moorea, French Polynesia<br />
Aug 17 to Aug 25, 2023<br />
Chief Scientist - Linda Wegley Kelly</p>
<p><em>Sample Collection. </em>At every site on the fore- or backreef, salinity and temperature were measured using Manta Probes multi-parameter sondes (Eureka Instruments). Approximately 1.2 liters (L) of reef water was collected via peristalsis through 0.2 micrometer (µm) polyethersulfone filter cartridges (Sterivex, Millipore, UK) directly into durable, food-grade, flexible pouches (1.5 liter, blended foil and EVOH (ethylene vinyl alcohol) plastic, AstraPouch) before being transported to the laboratory for further processing. Ten milliliters (mL) of whole seawater was also collected into a syringe using peristalsis and immediately fixed with paraformaldehyde at a final concentration of 0.5% for subsequent flow cytometry analysis of microbial abundances. The Sterivex filtrate was used to rinse bottles to collect samples for inorganic nutrients, which were frozen and stored at -40 degrees Celsius (°C). For dissolved organic carbon (DOC) measurements, Sterivex filtrate was used to triple sample-rinse borosilicate vials with Teflon septa caps before collecting 40 mL (for deployment 2 and 3 in duplicates). Additionally, 500 mL of filtrate was transferred to triple-rinsed polycarbonate bottles for solid-phase dissolved organic matter (DOM) extraction. DOC and DOM samples were acidified to pH 2 using trace metal grade HCl. Acidified DOM samples were solid-phase extracted using the multichannel pump operating at a flow rate of 18 mL per minute onto Bond Elut PPL resin cartridges (200 milligrams (mg) bed mass, Agilent 2105005, USA). After desalinating the resin with LC-MS grade water (Fisher Chemical, Belgium), the cartridges were dried using Ultra High Purity compressed N<sub>2</sub> gas and kept frozen at -40°C.</p>
<p><em>Biogeochemical Measurements. </em>Inorganic nutrients were analyzed using a Seal AA3 Segmented Flow Injection Autoanalyzer at the University of Hawai'i SOEST Laboratory for Analytical Biogeochemistry: Nitrate+nitrite (N+N) and silicate concentrations, ammonium, phosphate. Additionally, total dissolved nitrogen and total dissolved phosphorus were determined through separate injections, with UV and alkaline or acid persulfate in-line oxidation, respectively. DOC samples were analyzed using high-temperature platinum catalytic oxidation on a Shimadzu TOC-V at the University of Hawaii SOEST Laboratory. The analysis of fluorescent dissolved organic matter (fDOM) was conducted using a Horiba Aqualog scanning fluorometer. Samples for microbial cell concentrations were thawed and 200 microliters (µL) of each sample was stained with SYBR Green I stain for a final concentration of 1X. Bacterial cell counts were enumerated using an Attune Acoustic Focusing Cytometer (Applied Biosystems).</p>
<p>Note that while the instruments were the same from year to year, samples were not all run at the same time.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2118617 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2118617
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1949033 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1949033
completed
Craig E. Nelson
University of Hawaiʻi at Mānoa
805-705-3497
Center for Microbial Oceanography: Research & Education Dept of Oceanography & UH Sea Grant
Honolulu
HI
96822
USA
cen@hawaii.edu
pointOfContact
Linda Wegley Kelly
University of California-San Diego Scripps
858-246-4655
9500 Gilman Dr #0202
La Jolla
CA
92093-0202
USA
lwegley@ucsd.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Survey_Year
MooreaCode
SampleID
Experiment
Location
Campaign
ISO_DateTime_Local
ISO_DateTime_UTC
Time
Date
MASSIVE_mass_spec_sample_code
PPLVol
Notes
Replicate
Syringe_Autosampler
Syringe_Channel
Bag_Autosampler
Bag_Channel
Total_N
Total_P
Phosphate
Silicate
N_N
Ammonia
NPOC
Stdev_NPOC
M_to_C
BIX
HIX
FI
CobleA
CobleM
CobleC
CobleT
CobleB
Fpeak
Stedmon_D
Lignin
HBact
PEuk
Syn
Pro
Latitude
Longitude
Seal AA3 Segmented Flow Injection Autoanalyzer
Attune Acoustic Focusing Cytometer (Applied Biosystems)
Horiba Aqualog scanning fluorometer
Shimadzu TOC-V
Manta Probes multi-parameter sondes (Eureka Instruments)
theme
None, User defined
year
sample identification
experiment id
site
project
ISO_DateTime_Local
ISO_DateTime_UTC
time of day
date
volume
comments
replicate
instrument
Nitrogen
Phosphorus
phosphate
Silicate, Si(OH)4, silicic acid
nitrate plus nitrite
Ammonia
dissolved organic Carbon
ratio
fluorescence
abundance
latitude
longitude
featureType
BCO-DMO Standard Parameters
Bran+Luebbe / SEAL Analytical AutoAnalyzer 3 (AA3) continuous-flow analyzer
Flow Cytometer
Horiba Aqualog spectrofluorometer
Shimadzu TOC-V Analyzer
Water Quality Multiprobe
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Diel dynamics of dissolved organic matter production and remineralization as a driver of coral reef nutrient recycling
https://osprey.bco-dmo.org/project/855067
Collaborative Research: Diel dynamics of dissolved organic matter production and remineralization as a driver of coral reef nutrient recycling
<p>This project develops a core understanding of diel microbial ecology and biogeochemistry in coral reef ecosystems. It contextualizes how crucial nutrient recycling processes vary across gradients of coral cover and nutrient availability, two factors highlighted as the main drivers of reef decline in the Anthropocene, making the results useful to managers seeking to enhance ecosystem-based approaches to reef restoration. The investigators are collecting diel measurements of microbial and biogeochemical processes at coral reefs around Mo’orea at the Long Term Ecological Research site there (MCR-LTER). They are using established spatial gradients of benthic cover, from coral to macroalgal dominance, and nutrient inputs. Overall the research improves our understanding of how these key environmental factors influence diel microbe-DOM interactions and nutrient recycling. The ongoing macroalgal phase shifts observed at Mo’orea are hypothesized to be related to nutrient pollution, and this work directly informs understanding of how these changes are impacting nutrient cycling in the reefs of Mo’orea. The training of several undergraduate students and two graduate students, one in Biology and one in Oceanography is shared between two minority serving institutions of higher education. The project also supports active outreach programs with the Ocean Discovery Institute focused on engaging underrepresented high school students in ocean-oriented careers in San Diego, and with the UH College Sea Grant Program to support coral reef resilience initiatives locally in Hawai‘i.</p>
<p>Coral reefs exhibit some of the highest rates of primary production and decomposition of any ecosystem type yet persist in some of the most oligotrophic waters on the planet, implying tight recycling of macronutrients through organic matter. The last half century of work on the biogeochemistry of reefs have highlighted this bacterial decomposition of organic matter as a likely mechanism for maintaining nutrient retention and reef productivity. This project applies modern metagenomics and untargeted metabolomics to test clearly defined hypotheses of how diel microbe-DOM interactions drive nutrient recycling and retention in reefs. The investigators are first resolving coupled in situ diel dynamics of organic and inorganic C, N and P (using bulk elemental and spectroscopic methods), microbial abundances and population structures (using DNA sequencing and flow cytometry) and the chemical composition of DOM (using untargeted tandem mass spectrometry) in multiple reef habitats across a gradient of benthic cover and nutrient availability. These patterns inform the second in situ diel sampling campaign resolving the dynamic coupling of metabolic pathways (using metagenomics), exoenzymatic activity (using transcriptomics and enzyme assays) and transformations of specific metabolites (tracked via molecular networking) to distill common mechanisms of microbial organic matter decomposition that play a role in nutrient cycling. This project is being conducted within the Moorea Coral Reef Long Term Ecological Research program, leveraging a wealth of time series data on multiple reef habitats as well as contextualizing our in situ sampling with ongoing physical, geochemical and biological monitoring programs. By integrating cutting edge molecular approaches with well-established techniques in field ecology and microbial oceanography, this research program identifies key microbial and molecular players in the nutrient decomposition and remineralization processes long hypothesized to be central to maintaining healthy reefs.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Coral Diel
largerWorkCitation
project
eng; USA
oceans
-149.84258
-149.84258
-17.47722
-17.47722
2021-08-11
2023-08-25
Mo'orea, Moorea-Maiao, French Polynesia
0
BCO-DMO catalogue of parameters from Biogeochemical and microbial parameters collected during diel surveys on a coral reef at Mo'orea's North Shore in French Polynesia in 2021, 2022, and 2023
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/1003334.rdf
Name: Survey_Year
Units: unitless
Description: <p>Year of sample collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003335.rdf
Name: MooreaCode
Units: unitless
Description: <p>Numeric identifier for each individual sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003336.rdf
Name: SampleID
Units: unitless
Description: <p>Unique identifier for each individual sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003337.rdf
Name: Experiment
Units: unitless
Description: <p>Name of Experiment</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003338.rdf
Name: Location
Units: unitless
Description: <p>Sampling Location</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003339.rdf
Name: Campaign
Units: unitless
Description: <p>Name of the Campaign in which the Experiment was done</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003340.rdf
Name: ISO_DateTime_Local
Units: unitless
Description: <p>Local date and time converted to ISO 8601 format; Time zone = HST (UTC-10)</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003390.rdf
Name: ISO_DateTime_UTC
Units: unitless
Description: <p>Date and time converted to ISO 8601 format and converted to UTC time zone</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003341.rdf
Name: Time
Units: unitless
Description: <p>Time of collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003342.rdf
Name: Date
Units: unitless
Description: <p>Date of collection</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003343.rdf
Name: MASSIVE_mass_spec_sample_code
Units: unitless
Description: <p>Identification number for mass spectrometry data deposited on MassIVE (<a href="https://massive.ucsd.edu/">https://massive.ucsd.edu/</a>)</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003344.rdf
Name: PPLVol
Units: milliliters
Description: <p>Volume of seawater collected onto solid phase extraction resin</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003345.rdf
Name: Notes
Units: unitless
Description: <p>Notes on sampling</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003346.rdf
Name: Replicate
Units: unitless
Description: <p>Codes A and B or open and closed to denote paired samples</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003347.rdf
Name: Syringe_Autosampler
Units: unitless
Description: <p>Name of Autosampler used for Syringe FCM sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003348.rdf
Name: Syringe_Channel
Units: unitless
Description: <p>Channel of Autosampler used for Syringe FCM sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003349.rdf
Name: Bag_Autosampler
Units: unitless
Description: <p>Name of Autosampler used for Bag chemistry sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003350.rdf
Name: Bag_Channel
Units: unitless
Description: <p>Channel of Autosampler used for Bag chemistry sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003351.rdf
Name: Total_N
Units: micromoles per liter
Description: <p>Total Nitrogen concentration in micromoles per liter (umol/L)</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003352.rdf
Name: Total_P
Units: micromoles per liter
Description: <p>Total Phosphorus concentration in micromoles per liter (umol/L)</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003353.rdf
Name: Phosphate
Units: micromoles per liter
Description: <p>Phosphate concentration in micromoles per liter (umol/L)</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003354.rdf
Name: Silicate
Units: micromoles per liter
Description: <p>Silicate concentration in micromoles per liter (umol/L)</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003355.rdf
Name: N_N
Units: micromoles per liter
Description: <p>Nitrate + Nitrite concentration in micromoles per liter (umol/L)</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003356.rdf
Name: Ammonia
Units: micromoles per liter
Description: <p>Ammonia concentration in the water sample in micromoles per liter (umol/L)</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003357.rdf
Name: NPOC
Units: micromoles per liter
Description: <p>Dissolved Organic Carbon concentration in micromoles per liter (umol/L)</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003358.rdf
Name: Stdev_NPOC
Units: micromoles per liter
Description: <p>Standard deviation of Dissolved Organic Carbon concentration, micromoles per liter (umol/L)</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003359.rdf
Name: M_to_C
Units: Raman Fluorescence Units of Water
Description: <p>Humic to Protein-like ratio in Raman Fluorescence Units of Water</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003360.rdf
Name: BIX
Units: Raman Fluorescence Units of Water
Description: <p>Biological Index in Raman Fluorescence Units of Water</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003361.rdf
Name: HIX
Units: Raman Fluorescence Units of Water
Description: <p>Humification Index in Raman Fluorescence Units of Water</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003362.rdf
Name: FI
Units: Raman Fluorescence Units of Water
Description: <p>Fluorescence Index in Raman Fluorescence Units of Water</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003363.rdf
Name: CobleA
Units: Raman Fluorescence Units of Water
Description: <p>Ultra Violet Humic-like component in Raman Fluorescence Units of Water</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003364.rdf
Name: CobleM
Units: Raman Fluorescence Units of Water
Description: <p>Marine Humic-like component in Raman Fluorescence Units of Water</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003365.rdf
Name: CobleC
Units: Raman Fluorescence Units of Water
Description: <p>Visible Humic-like component in Raman Fluorescence Units of Water</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003366.rdf
Name: CobleT
Units: Raman Fluorescence Units of Water
Description: <p>Tyrosine-like component in Raman Fluorescence Units of Water</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003367.rdf
Name: CobleB
Units: Raman Fluorescence Units of Water
Description: <p>Tryptophan-like: Tryptophan-like component in Raman Fluorescence Units of Water</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003368.rdf
Name: Fpeak
Units: Raman Fluorescence Units of Water
Description: <p>Generic Humic-like component in Raman Fluorescence Units of Water</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003369.rdf
Name: Stedmon_D
Units: Raman Fluorescence Units of Water
Description: <p>Fulvic Acid-like: Fulvic Acid-like component in Raman Fluorescence Units of Water</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003370.rdf
Name: Lignin
Units: Raman Fluorescence Units of Water
Description: <p>Lignin-like component in Raman Fluorescence Units of Water</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003371.rdf
Name: HBact
Units: cells per microliter
Description: <p>Heterotrophic bacterioplankton concentration in cells per microliter (cells/uL)</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003372.rdf
Name: PEuk
Units: cells per microliter
Description: <p>PicoEukaryotes cell concentration in cells per microliter (cells/uL)</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003373.rdf
Name: Syn
Units: cells per microliter
Description: <p>Synechococcus cell concentration in cells per microliter (cells/uL)</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003374.rdf
Name: Pro
Units: cells per microliter
Description: <p>Prochlorococcus cell concentration in cells per microliter (cells/uL)</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003375.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude of the general sampling area</p>
http://lod.bco-dmo.org/id/dataset-parameter/1003377.rdf
Name: Longitude
Units: decimal degrees
Description: <p>Longitude of the general sampling area</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
191411
https://datadocs.bco-dmo.org/file/LAxlEJ5sNMROqm/1003286_v1_moorea_n_shore_diel_2021-2023.csv
1003286_v1_moorea_n_shore_diel_2021-2023.csv
Primary data file for dataset ID 1003286, version 1
download
https://osprey.bco-dmo.org/dataset/1003286/data/download
download
onLine
dataset
<p><em>Sampling Dates:</em><br />
2021:<br />
Gump Station, Moorea, French Polynesia<br />
Aug 10 to Aug 19, 2021<br />
Chief Scientist - Linda Wegley Kelly (SIO, lwegley@ucsd.edu)</p>
<p>2022:<br />
Gump Station, Moorea, French Polynesia<br />
Mar 29 to Apr 15, 2022<br />
Chief Scientist - Linda Wegley Kelly</p>
<p>2023:<br />
Gump Station, Moorea, French Polynesia<br />
Aug 17 to Aug 25, 2023<br />
Chief Scientist - Linda Wegley Kelly</p>
<p><em>Sample Collection. </em>At every site on the fore- or backreef, salinity and temperature were measured using Manta Probes multi-parameter sondes (Eureka Instruments). Approximately 1.2 liters (L) of reef water was collected via peristalsis through 0.2 micrometer (µm) polyethersulfone filter cartridges (Sterivex, Millipore, UK) directly into durable, food-grade, flexible pouches (1.5 liter, blended foil and EVOH (ethylene vinyl alcohol) plastic, AstraPouch) before being transported to the laboratory for further processing. Ten milliliters (mL) of whole seawater was also collected into a syringe using peristalsis and immediately fixed with paraformaldehyde at a final concentration of 0.5% for subsequent flow cytometry analysis of microbial abundances. The Sterivex filtrate was used to rinse bottles to collect samples for inorganic nutrients, which were frozen and stored at -40 degrees Celsius (°C). For dissolved organic carbon (DOC) measurements, Sterivex filtrate was used to triple sample-rinse borosilicate vials with Teflon septa caps before collecting 40 mL (for deployment 2 and 3 in duplicates). Additionally, 500 mL of filtrate was transferred to triple-rinsed polycarbonate bottles for solid-phase dissolved organic matter (DOM) extraction. DOC and DOM samples were acidified to pH 2 using trace metal grade HCl. Acidified DOM samples were solid-phase extracted using the multichannel pump operating at a flow rate of 18 mL per minute onto Bond Elut PPL resin cartridges (200 milligrams (mg) bed mass, Agilent 2105005, USA). After desalinating the resin with LC-MS grade water (Fisher Chemical, Belgium), the cartridges were dried using Ultra High Purity compressed N<sub>2</sub> gas and kept frozen at -40°C.</p>
<p><em>Biogeochemical Measurements. </em>Inorganic nutrients were analyzed using a Seal AA3 Segmented Flow Injection Autoanalyzer at the University of Hawai'i SOEST Laboratory for Analytical Biogeochemistry: Nitrate+nitrite (N+N) and silicate concentrations, ammonium, phosphate. Additionally, total dissolved nitrogen and total dissolved phosphorus were determined through separate injections, with UV and alkaline or acid persulfate in-line oxidation, respectively. DOC samples were analyzed using high-temperature platinum catalytic oxidation on a Shimadzu TOC-V at the University of Hawaii SOEST Laboratory. The analysis of fluorescent dissolved organic matter (fDOM) was conducted using a Horiba Aqualog scanning fluorometer. Samples for microbial cell concentrations were thawed and 200 microliters (µL) of each sample was stained with SYBR Green I stain for a final concentration of 1X. Bacterial cell counts were enumerated using an Attune Acoustic Focusing Cytometer (Applied Biosystems).</p>
<p>Note that while the instruments were the same from year to year, samples were not all run at the same time.</p>
Specified by the Principal Investigator(s)
<p>The shared data is unprocessed.</p>
Specified by the Principal Investigator(s)
- Imported three original CSV files, Diel2021_BCODMO_Wegley_Kelly_Nelson.17Dec2025.csv, Diel2022_BCODMO_Wegley_Kelly_Nelson.17Dec2025.csv, and Diel2023_BCODMO_Wegley_Kelly_Nelson.17Dec2025.csv into the BCO-DMO data processing system.
- Treated "NA" as a missing data value (missing data are empty/blank in the final CSV file).
- Added a constant integer column Survey_Year with values 2021, 2022, and 2023 to their respective rows.
- Renamed multiple columns to standardize names across files and to comply with BCO-DMO naming conventions.
- Concatenated all three tables into a single combined table, mapping all 40 columns by name.
- Computed a new "ISO_DateTime_Local" datetime column by combining "Time" (format %H:%M) and "Date" (format %m/%d/%Y) columns, output formatted as %Y-%m-%dT%H:%M.
- Computed a new "ISO_DateTime_UTC" datetime column by combining the same "Time" and "Date" columns, interpreting the input as HST timezone and converting output to UTC, formatted as %Y-%m-%dT%H:%MZ.
- Added constant Latitude column ("-17.47722") and Longitude column ("-149.84258") to all rows, representing the general sampling area coordinates.
- Saved the final file as "1003286_v1_moorea_n_shore_diel_2021-2023.csv".
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Seal AA3 Segmented Flow Injection Autoanalyzer
Seal AA3 Segmented Flow Injection Autoanalyzer
PI Supplied Instrument Name: Seal AA3 Segmented Flow Injection Autoanalyzer PI Supplied Instrument Description:Inorganic nutrients were analyzed using a Seal AA3 Segmented Flow Injection Autoanalyzer at the University of Hawai'i SOEST Laboratory for Analytical Biogeochemistry. Instrument Name: Bran+Luebbe / SEAL Analytical AutoAnalyzer 3 (AA3) continuous-flow analyzer Instrument Short Name:AA3 AutoAnalyzer Instrument Description: The AutoAnalyzer 3 (AA3) is a segmented continuous-flow analyzer (continuous flow analyzer, CFA) used for automated colorimetric analysis of dissolved nutrients and other analytes in environmental, seawater, freshwater, wastewater, soil, and agricultural samples. The AA3 was originally manufactured by Bran+Luebbe and, following acquisition of the product line in 2006, has continued to be manufactured and supported by SEAL Analytical. The AA3 is the third-generation instrument in the Technicon AutoAnalyzer family and is widely used for determination of nitrate, nitrite, ammonium, phosphate, silicate, and other dissolved nutrients. See the description from the manufacturer. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/
Attune Acoustic Focusing Cytometer (Applied Biosystems)
Attune Acoustic Focusing Cytometer (Applied Biosystems)
PI Supplied Instrument Name: Attune Acoustic Focusing Cytometer (Applied Biosystems) PI Supplied Instrument Description:Bacterial cell counts were enumerated using an Attune Acoustic Focusing Cytometer (Applied Biosystems). Instrument Name: Flow Cytometer Instrument Short Name:Flow Cytometer Instrument Description: Flow cytometers (FC or FCM) are automated instruments that quantitate properties of single cells, one cell at a time. They can measure cell size, cell granularity, the amounts of cell components such as total DNA, newly synthesized DNA, gene expression as the amount messenger RNA for a particular gene, amounts of specific surface receptors, amounts of intracellular proteins, or transient signalling events in living cells. Description from: http://www.bio.umass.edu/micro/immunology/facs542/facswhat.htm Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB37/
Horiba Aqualog scanning fluorometer
Horiba Aqualog scanning fluorometer
PI Supplied Instrument Name: Horiba Aqualog scanning fluorometer PI Supplied Instrument Description:The analysis of fluorescent dissolved organic matter (fDOM) was conducted using a Horiba Aqualog scanning fluorometer. Instrument Name: Horiba Aqualog spectrofluorometer Instrument Short Name:Horiba Aqualog Instrument Description: A benchtop optical spectrometer suitable for measuring coloured dissolved organic matter (CDOM). Outputs include absorbance spectra, fluorescence emission spectra, and fluorescence excitation-emission matrices. This instrument simultaneously measures absorbance spectra and fluorescence Excitation-Emission Matrices. It employs the Absorbance-Transmission Excitation Emission Matrix (A-TEEM) technique to acquire an Excitation Emission Matrix.
Shimadzu TOC-V
Shimadzu TOC-V
PI Supplied Instrument Name: Shimadzu TOC-V PI Supplied Instrument Description:DOC samples were analyzed using high-temperature platinum catalytic oxidation on a Shimadzu TOC-V at the University of Hawaii SOEST Laboratory. Instrument Name: Shimadzu TOC-V Analyzer Instrument Short Name:Shimadzu TOC-V Instrument Description: A Shimadzu TOC-V Analyzer measures DOC by high temperature combustion method. Community Standard Description: http://onto.nerc.ac.uk/CAST/124
Manta Probes multi-parameter sondes (Eureka Instruments)
Manta Probes multi-parameter sondes (Eureka Instruments)
PI Supplied Instrument Name: Manta Probes multi-parameter sondes (Eureka Instruments) PI Supplied Instrument Description:Salinity and temperature were measured using Manta Probes multi-parameter sondes (Eureka Instruments). Instrument Name: Water Quality Multiprobe Instrument Short Name:Water Quality Multiprobe Instrument Description: An instrument which measures multiple water quality parameters based on the sensor configuration.