http://lod.bco-dmo.org/id/dataset/1004007
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2026-08-06
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Dissolved iron concentrations from an iron incubation experiment using upwelled waters in the California Current System during the PUPCYCLE II cruise in May and June 2023
2026-08-06
publication
2026-08-06
revision
BCO-DMO Linked Data URI
2026-08-06
creation
http://lod.bco-dmo.org/id/dataset/1004007
Adrian Marchetti
University of North Carolina at Chapel Hill
principalInvestigator
Claire P. Till
Cal Poly Humboldt (formerly Humboldt State University)
principalInvestigator
Ralph C. Till
Cal Poly Humboldt (formerly Humboldt State University)
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Till, C. P., Till, R. C., Marchetti, A. (2026) Dissolved iron concentrations from an iron incubation experiment using upwelled waters in the California Current System during the PUPCYCLE II cruise in May and June 2023. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-08-06 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/1004007 [access date]
Dissolved Fe concentrations from PUPCYCLE II Dataset Description: Methods and Sampling: <p>Data collection took place on the R/V Sally Ride from May 29th to June 10th, 2023. To simulate upwelling conditions under different iron treatments, an onboard incubation experiment was conducted. Seawater for the incubation experiment was collected within the northern CCS, off the southern coast of Oregon at 43°02'42.7"N, 124°33'07.2"W. The collected seawater was deemed as freshly upwelled water and taken from a depth of 55 m – which corresponds to a depth slightly below the euphotic zone receiving less than 1% irradiance – using trace-metal clean techniques on May 30th, 2023, 13:30 GMT. The seawater was pumped and homogenized using trace metal clean techniques, then transferred into a total of thirty 20 L low-density polyethylene cubitainers. Three cubitainers were immediately harvested for the initial timepoint (T0). The remaining twenty-seven cubitainers were assigned treatments: nine were unamended (labeled Ctrl), nine were amended with 5 nM FeCl2 (labeled +Fe), and nine were amended with 200 nM desferrioxamine B,&nbsp;a strong iron chelator that inhibits dissolved iron uptake (labeled DFB). The cubitainers were placed in an on-deck incubator covered with two layers of neutral density screening to simulate 26% of incidence irradiance supplied with flow-through surface seawater to maintain ambient surface temperature. Three cubitainers from each treatment were harvested for each of the three subsequent timepoints: 48 hours (T1), 168 hours (T2), and 264 hours (T3) after incubation.</p>
<p><strong>Iron Incubation Experimental Set-Up. </strong>The seawater for the iron incubation experiment was pumped into a positive pressure trace metal clean laboratory and then into 50-gallon acid-washed high-density polyethylene (HDPE) drums. Seawater in these drums was homogenized using a Wilden air-operated double diaphragm pump made of polytetrafluoroethylene and acid-washed HDPE tubing. The seawater was then transferred into a total of thirty 20 L low-density polyethylene cubitainers.&nbsp;</p>
<p><strong>Dissolved Iron Sampling. </strong>Samples for measurement of dissolved iron were filtered directly from the cubitainer through an acid-cleaned Acropak supor membrane cartridge filter with 0.2 micrometer pore size. Filtered samples were collected in acid-cleaned LDPE bottles, which were rinsed three times with sample prior to filling. Samples were acidified at sea with Optima hydrochloric acid to a pH of ~1.8.&nbsp;</p>
<p><strong>Dissolved Iron Analysis. </strong>Samples were analyzed post-cruise in a trace metal clean room using the methods of Lohan et al. (2006) with modifications as described in Biller et al. (2013). This method involves pre-concentrating iron on a chelating resin; we followed Biller et al. (2013) and used Toyopearl Chelate-650 resin, which recovers &gt;93% of Fe(III) at pH 2. We added a small amount of Optima ammonium hydroxide to each sample before analysis to increase the pH from 1.8 to 2. We also added hydrogen peroxide (10 micromolar) to each sample ten minutes before analysis to ensure quantitative oxidation of all Fe(II) to Fe(III), which Lohan et al (2005) found to be effective. After pre-concentration, the iron is eluted into a reaction stream with the colorimetric agent N,N-dimethyl-p-phenylenediaminedihydrochloride (DPD), which turns deeper pink the more iron is present. The signal is detected with a flow-through spectrophotometer.&nbsp;</p>
<p>Standards were made by spiking low metal seawater with known amounts of added iron and creating a standard addition curve. Blanks were assessed by analyzing acidified MilliQ as a sample. Reference samples (Geotraces GSC and D1) measured in the same analytical runs as the samples were in line with consensus values.&nbsp;</p>
<p><strong>Note about the DFB treatments: </strong>high concentrations (&gt;10nM) of dissolved Fe were measured in the treatments with added DFB. We think this is likely because the strong chelator solubilized Fe. It is also possible there was some Fe in the DFB spike. Regardless, the dissolved Fe present in the DFB treatments was almost certainly not biologically available, as it was chelated with DFB.&nbsp;<br />
<br />
Notes about methods papers cited:<br />
*&nbsp;Lohan et al. (2005, doi: 10.1016/j.aca.2004.09.005) -&nbsp;This paper shows that 10 micromolar H2O2 and ten minutes is sufficient to quantitatively oxidize Fe(II) to Fe(III) in acidified seawater<br />
*&nbsp;Lohan et al. (2006, doi: 10.4319/lom.2006.4.164) -&nbsp;The main methods paper, but we used some modifications as in Biller et al., 2013.&nbsp;<br />
*&nbsp;Biller et al. (2023, doi: 10.1016/j.csr.2013.07.003) -&nbsp;The paper that has the modifications we used to Lohan et al 2006's method.&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1751805 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1751805
onGoing
Adrian Marchetti
University of North Carolina at Chapel Hill
919-843-3473
123 South Rd.
Chapel Hill
NC
27514
USA
amarchet@email.unc.edu
pointOfContact
Claire P. Till
Cal Poly Humboldt (formerly Humboldt State University)
707-826-3266
Chemistry Department 1 Harpst Street
Arcata
CA
95521
USA
claire.till@humboldt.edu
pointOfContact
Ralph C. Till
Cal Poly Humboldt (formerly Humboldt State University)
rct177@humboldt.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Time_Stamp
Treatment
Replicate
Sample_Name
Fe_Conc
Wilden air-operated double diaphragm pump
Varian model 340 UV-Vis detector
theme
None, User defined
sample time
treatment
replicate
sample identification
Iron
featureType
BCO-DMO Standard Parameters
Pump
Spectrophotometer
instrument
BCO-DMO Standard Instruments
SR2311
service
Deployment Activity
California Current System
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
CAREER: An integrated molecular and physiological approach to examining the dynamics of upwelled phytoplankton in current and changing oceans
https://osprey.bco-dmo.org/project/768006
CAREER: An integrated molecular and physiological approach to examining the dynamics of upwelled phytoplankton in current and changing oceans
<p><em>NSF Award Abstract:</em><br />
Upwelling zones are hotspots of photosynthesis that are very dynamic in space and time. Microscopic algae, known as phytoplankton, bloom when deep, nutrient-rich waters are upwelled into sunlit surface layers of the ocean, providing nourishment that supports productive food webs and draws down carbon dioxide (CO2) from the atmosphere to the deep ocean. Photosynthetic microbes in these regions must constantly adapt to changes in their chemical and physical environments. For example, subsurface populations respond to changes in light as they approach the surface. When upwelled waters move offshore, cells sink out of the illuminated zone, establishing seed populations that remain inactive until the next upwelling event. This process is called the upwelling conveyor belt cycle (UCBC). How phytoplankton respond to these changes in environmental conditions and how they may influence their nutrient requirements remains unknown. With future ocean changes predicted to alter seawater chemistry, including ocean acidification and decreased iron availability, some phytoplankton groups may be more vulnerable than others. Accompanying educational activities provide learning experiences to enhance understanding and awareness of marine microbes. The development of a research hub at UNC aims to provide infrastructure and support for scientists and students conducting research on environmental genomics. A laboratory component for an upper-level undergraduate course focused on marine phytoplankton is being developed. Educational outreach activities to broader communities include creation of a lesson plan on phytoplankton in upwelling zones and a virtual research cruise experience for middle-school students, as well as a hands-on lab activity for a local museum focused on marine phytoplankton and the important roles they play in shaping our planet.</p>
<p>The project examines how phytoplankton respond at the molecular and physiological level to the different UCBC stages, which seed populations (i.e., surface versus subsurface) contribute most to phytoplankton blooms during upwelling events of varying intensity, how phytoplankton elemental compositions are altered throughout UCBC stages, and how future predicted ocean conditions will affect the phytoplankton responses to UCBC conditions. This project contains both laboratory and fieldwork. In the laboratory, phytoplankton isolates recently obtained from upwelling regions are exposed to simulated UCBC conditions to examine changes in gene expression, growth and photosynthetic characteristics and elemental composition. Cultures are subjected to both current and future ocean conditions, including reduced iron availability and higher CO2. In the field, research cruises within upwelling regions study the dynamics of natural phytoplankton communities (both surface and subsurface) experiencing upwelling and relaxation and within simulated upwelling incubation experiments. Knowledge of how phytoplankton are affected by UCBC conditions at an integrated molecular, physiological and elemental level under both current and future scenarios is imperative for the proper conservation and management of these critically important ecosystems.</p>
Upwelled Phytoplankton Dynamics
largerWorkCitation
project
eng; USA
oceans
California Current System
-124.552
-124.552
43.0452
43.0452
2023-05-29
2023-06-10
California Upwelling Zone
0
BCO-DMO catalogue of parameters from Dissolved iron concentrations from an iron incubation experiment using upwelled waters in the California Current System during the PUPCYCLE II cruise in May and June 2023
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/1004320.rdf
Name: Time_Stamp
Units: unitless
Description: <p>The timepoint of sampling the incubation: 48 hours (T1), 168 hours (T2), and 264 hours (T3).</p>
http://lod.bco-dmo.org/id/dataset-parameter/1004321.rdf
Name: Treatment
Units: unitless
Description: <p>Experimental treatment where Ctrl = unamended control; plus Fe = amended with 5nM FeCl2; plus DFB (low Fe) = amended with 200 nM desferrioxamine B</p>
http://lod.bco-dmo.org/id/dataset-parameter/1004322.rdf
Name: Replicate
Units: unitless
Description: <p>Each treatment at each timepoint was in triplicate. The three different cubitainers for the triplicate measurements are indicated with A, B, and C</p>
http://lod.bco-dmo.org/id/dataset-parameter/1004323.rdf
Name: Sample_Name
Units: unitless
Description: <p>Incubation samples were identified with a Q number to unambiguously identify each sample by treatment, replicate and timepoint.</p>
http://lod.bco-dmo.org/id/dataset-parameter/1004324.rdf
Name: Fe_Conc
Units: nanomoles per kilogram (nmol/kg)
Description: <p>The concentration of dissolved iron (less than 0.2 micrometers)</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://osprey.bco-dmo.org/dataset/1004007/data/download
download
onLine
dataset
<p>Data collection took place on the R/V Sally Ride from May 29th to June 10th, 2023. To simulate upwelling conditions under different iron treatments, an onboard incubation experiment was conducted. Seawater for the incubation experiment was collected within the northern CCS, off the southern coast of Oregon at 43°02'42.7"N, 124°33'07.2"W. The collected seawater was deemed as freshly upwelled water and taken from a depth of 55 m – which corresponds to a depth slightly below the euphotic zone receiving less than 1% irradiance – using trace-metal clean techniques on May 30th, 2023, 13:30 GMT. The seawater was pumped and homogenized using trace metal clean techniques, then transferred into a total of thirty 20 L low-density polyethylene cubitainers. Three cubitainers were immediately harvested for the initial timepoint (T0). The remaining twenty-seven cubitainers were assigned treatments: nine were unamended (labeled Ctrl), nine were amended with 5 nM FeCl2 (labeled +Fe), and nine were amended with 200 nM desferrioxamine B,&nbsp;a strong iron chelator that inhibits dissolved iron uptake (labeled DFB). The cubitainers were placed in an on-deck incubator covered with two layers of neutral density screening to simulate 26% of incidence irradiance supplied with flow-through surface seawater to maintain ambient surface temperature. Three cubitainers from each treatment were harvested for each of the three subsequent timepoints: 48 hours (T1), 168 hours (T2), and 264 hours (T3) after incubation.</p>
<p><strong>Iron Incubation Experimental Set-Up. </strong>The seawater for the iron incubation experiment was pumped into a positive pressure trace metal clean laboratory and then into 50-gallon acid-washed high-density polyethylene (HDPE) drums. Seawater in these drums was homogenized using a Wilden air-operated double diaphragm pump made of polytetrafluoroethylene and acid-washed HDPE tubing. The seawater was then transferred into a total of thirty 20 L low-density polyethylene cubitainers.&nbsp;</p>
<p><strong>Dissolved Iron Sampling. </strong>Samples for measurement of dissolved iron were filtered directly from the cubitainer through an acid-cleaned Acropak supor membrane cartridge filter with 0.2 micrometer pore size. Filtered samples were collected in acid-cleaned LDPE bottles, which were rinsed three times with sample prior to filling. Samples were acidified at sea with Optima hydrochloric acid to a pH of ~1.8.&nbsp;</p>
<p><strong>Dissolved Iron Analysis. </strong>Samples were analyzed post-cruise in a trace metal clean room using the methods of Lohan et al. (2006) with modifications as described in Biller et al. (2013). This method involves pre-concentrating iron on a chelating resin; we followed Biller et al. (2013) and used Toyopearl Chelate-650 resin, which recovers &gt;93% of Fe(III) at pH 2. We added a small amount of Optima ammonium hydroxide to each sample before analysis to increase the pH from 1.8 to 2. We also added hydrogen peroxide (10 micromolar) to each sample ten minutes before analysis to ensure quantitative oxidation of all Fe(II) to Fe(III), which Lohan et al (2005) found to be effective. After pre-concentration, the iron is eluted into a reaction stream with the colorimetric agent N,N-dimethyl-p-phenylenediaminedihydrochloride (DPD), which turns deeper pink the more iron is present. The signal is detected with a flow-through spectrophotometer.&nbsp;</p>
<p>Standards were made by spiking low metal seawater with known amounts of added iron and creating a standard addition curve. Blanks were assessed by analyzing acidified MilliQ as a sample. Reference samples (Geotraces GSC and D1) measured in the same analytical runs as the samples were in line with consensus values.&nbsp;</p>
<p><strong>Note about the DFB treatments: </strong>high concentrations (&gt;10nM) of dissolved Fe were measured in the treatments with added DFB. We think this is likely because the strong chelator solubilized Fe. It is also possible there was some Fe in the DFB spike. Regardless, the dissolved Fe present in the DFB treatments was almost certainly not biologically available, as it was chelated with DFB.&nbsp;<br />
<br />
Notes about methods papers cited:<br />
*&nbsp;Lohan et al. (2005, doi: 10.1016/j.aca.2004.09.005) -&nbsp;This paper shows that 10 micromolar H2O2 and ten minutes is sufficient to quantitatively oxidize Fe(II) to Fe(III) in acidified seawater<br />
*&nbsp;Lohan et al. (2006, doi: 10.4319/lom.2006.4.164) -&nbsp;The main methods paper, but we used some modifications as in Biller et al., 2013.&nbsp;<br />
*&nbsp;Biller et al. (2023, doi: 10.1016/j.csr.2013.07.003) -&nbsp;The paper that has the modifications we used to Lohan et al 2006's method.&nbsp;</p>
Specified by the Principal Investigator(s)
<p>Data was processed in Microsoft Excel. Blanks were subtracted from the sample signals, and the slopes of the standard addition curves were used to convert from absorbance to concentration. Drift in sensitivity was adjusted for by running a quality control sample many times throughout a sequence: any changes to its signal were linearly extrapolated and applied to the samples throughout the sequence.&nbsp;</p>
Specified by the Principal Investigator(s)
- Imported data from source file "dFe data Adrian's incubation for BCODMO.xlsx" into the BCO-DMO system.
- Renamed columns to conform with FAIR principles and BCO-DMO naming conventions. The only allowed characters are A-Z,a-z,0-9, and underscores. No spaces, hyphens, commas, parentheses, or Greek letters.
- Set types as string and numeric
- Exported data as CSV file titled "1004007_v1_iron_concentrations_pupcycle2.csv"
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Wilden air-operated double diaphragm pump
Wilden air-operated double diaphragm pump
PI Supplied Instrument Name: Wilden air-operated double diaphragm pump PI Supplied Instrument Description:A Wilden air-operated double diaphragm pump made of polytetrafluoroethylene and acid-washed HDPE tubing was used to homogenize the seawater in the drums Instrument Name: Pump Instrument Short Name: Instrument Description: A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps
Varian model 340 UV-Vis detector
Varian model 340 UV-Vis detector
PI Supplied Instrument Name: Varian model 340 UV-Vis detector PI Supplied Instrument Description:The flow through spectrophotometer used for these samples was a Varian model 340 UV-Vis detector at 514 nm. Instrument Name: Spectrophotometer Instrument Short Name:Spectrophotometer Instrument Description: An instrument used to measure the relative absorption of electromagnetic radiation of different wavelengths in the near infra-red, visible and ultraviolet wavebands by samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB20/
Cruise: SR2311
SR2311
R/V Sally Ride
Community Standard Description
International Council for the Exploration of the Sea
R/V Sally Ride
vessel
SR2311
Adrian Marchetti
University of North Carolina at Chapel Hill
R/V Sally Ride
Community Standard Description
International Council for the Exploration of the Sea
R/V Sally Ride
vessel