http://lod.bco-dmo.org/id/dataset/1004161 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2026-08-07 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Dissolved nutrient data from samples collected from 1998 through 2025 from the surface waters of Tatoosh Island, Makah Reservation, Washington, USA 2026-08-11 publication 2026-08-11 revision Woods Hole Oceanographic Institution (WHOI BCO-DMO) 2026-08-11 publication https://doi.org/10.26008/1912/bco-dmo.1004161.1 Catherine Ann Pfister University of Chicago principalInvestigator John Timothy Wootton University of Chicago principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Pfister, C. A., Wootton, J. T. (2026) Dissolved nutrient data from samples collected from 1998 through 2025 from the surface waters of Tatoosh Island, Makah Reservation, Washington, USA. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-08-11 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.1004161.1 [access date] Tatoosh Island Nutrient Data Dataset Description: Methods and Sampling: &lt;p&gt;All censusing was done from shore or with small boats. Sampling stations were established on the shoreline of Tatoosh Island and adjacent offshore sites to the east of the island beginning in August 1998. Seven of the sites (Finger, Glacier Control, Hedophyllum Cove, Ladd's Finger, Simon's Landing, Strawberry Point, Toad Point) corresponded to wave-exposed shores on the periphery of the island used in a prior nutrient manipulation experiment (Wootton et al. 1996), and an eighth (Northwest Point) was another wave-exposed shore selected to expand peripheral coverage of the island. Two additional relatively wave-sheltered sites were also added to the sampling, both located inside extensive kelp beds: Main Beach and Strawberry Draw. Water at all these sites was collected 2-3 hours after a strong spring tide on a day when the investigators were departing from the island to facilitate rapid sample freezing. Water samples were collected in 2001, 2015, 2016, and 2022 at an additional shoreline site (Hydrolab Pool) subject to intensive sampling of ocean conditions. In 2000, four offshore sampling sites were established at varying distances from the island (Inside Kelp, ~30 meters (m); Outside Kelp, ~100 m; Gut, ~0.6 kilometers (km); Mushroom Rocks, ~ 1.8 km from island, ~0.5 km from the mainland). Water at the offshore sites was collected by boat approximately 4-5 hours after the tide. Water was extracted from the ocean at the surface using 50-milliliter (mL) syringes, which were first rinsed in the water at the site before a sample was drawn. The sample was then pushed through a syringe filter loaded with a disk of Whatman GF/F glass fiber filter paper, and collected in 40 mL acid-washed Nalgene bottles, leaving enough headspace to allow water expansion when frozen.&lt;/p&gt; &lt;p&gt;Water samples were kept in coolers following collection and immediately frozen at -20 degrees Celsius upon return to the mainland field base. Frozen water samples were express shipped to the University of Washington Ocean Chemistry Laboratory, where they were analyzed following the methods outlined in (UNESCO 1994) using a Seal Analytical AA3 nutrient autoanalyzer. In 2025, the analysis of samples from all dates/sites was carried out by the Moss Landing Marine Laboratory Environmental Analytical Laboratory, by colorimetric analysis using a Lachat QuickChem 8000 Flow Injection Analyzer and 50 mL Falcon Tubes as sample containers. We sent duplicate samples from 29 May 2025 and 23 August 2025 to the University of Washington Marine Chemistry Laboratory for comparative purposes.&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0928232 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0928232 Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1851489 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1851489 Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0117801 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0117801 Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0452687 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0452687 Funding provided by NSF Division of Environmental Biology (NSF DEB) Award Number: DEB-0919420 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0919420 Funding provided by NSF Division of Environmental Biology (NSF DEB) Award Number: DEB-1556874 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1556874 Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2329475 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2329475 Funding provided by National Oceanic and Atmospheric Administration (NOAA) Award Number: NOAA-COCA NA16OAR4310155 Funding provided by Washington State Department of Natural Resources (WDNR) Award Number: WDNR 93099282 Funding provided by Washington State Department of Natural Resources (WDNR) Award Number: WDNR 93100399 completed Catherine Ann Pfister University of Chicago 773-834-0071 Department of Ecology and Evolution 1101 E 57th St Chicago IL 60637 USA cpfister@uchicago.edu pointOfContact John Timothy Wootton University of Chicago 773-202-2773 Dept. of Ecology and Evolution 1101 East 57th St. Chicago IL 60637 USA twootton@uchicago.edu pointOfContact asNeeded Dataset Version: 1 Unknown ISO_DateTime_Local ISO_DateTime_UTC Date_local Time_local Site Latitude Longitude NO3 NO2 NH4 PO4 SiOH4 Lab Lachat QuickChem 8000 Flow Injection Analyzer Seal Analytical AA3 nutrient autoanalyzer theme None, User defined ISO_DateTime_Local ISO_DateTime_UTC date time of day site latitude longitude Nitrate Nitrite Ammonium reactive phosphorus (PO4) Silicic acid laboratory featureType BCO-DMO Standard Parameters Lachat QuikChem 8000 flow injection analyzer and Ion Chromatography (IC) system Seal Analytical AutoAnalyser 3HR instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. The role of microbes in driving productivity and carbon fixation in seaweeds https://pfisterlab.uchicago.edu/basic-page/ The role of microbes in driving productivity and carbon fixation in seaweeds <p><em>NSF Award Abstract:</em><br /> It is increasingly recognized that species in the ocean host a diverse set of microbes and that these microbes can determine the functioning of the host. Yet, the discovery of microbial taxa in nature far outpaces our understanding of their functions. Primary producers in the ocean show rich microbial communities and may contribute to the significant role that macrophytes play in coastal communities. This project focuses on a species of canopy kelp that is a foundational species along the shores of the northeast Pacific Ocean, the bull kelp <em>Nereocystis luetkeana</em>. The research explores the role of a diverse set of microbes in association with bull kelp. Using experimental manipulations of newly isolated microbial taxa from kelp, tests of whether interactions are positive, negative, or neutral are performed. Specific assays of whether microbes provision vitamins and enhance access to nutrients are carried out, as well as tests of whether kelp exudates benefit microbial metabolism and growth. Working directly with Tribal youth through an internship program, as well as with undergraduate, post-baccalaureate, and graduate students, helps train the next generation of ocean scientists. Communication channels with Tribal and State governments inform efforts to understand kelp declines. Host-microbe interactions are a component of the persistence of ocean species and this research informs the factors that underly these relationships.</p> <p>The advent of DNA sequencing, imaging, and other molecular approaches have revealed that many key species in the ocean are a 'holobiome', and their fate is entwined with the microbes they host. Yet, this discovery of microbial taxa in nature far outpaces our understanding of microbial function. <em>Nereocystis luetkeana</em>, or bull kelp, and the bacteria the investigators have isolated from the kelp surface, present an opportunity to quantify host-microbe interactions, including how stressors of host health, such as ocean warming, alter these interactions. Experiments tracing stable nitrogen isotopes and manipulations with hosts and bacterial isolates are used to investigate possible exchanges and the currencies underlying interactions. Metabolomic, proteomic, and genomic analyses are used to quantify host-microbe interactions and linkage between taxa and their functions. Key microbial metabolisms being investigated include nutrient provisioning, vitamin synthesis, and the response to reactive oxygen species, among others. The demonstrated alteration of host-microbe interactions in the ocean when hosts are stressed suggest the findings of this study have application to understanding the factors that promote persistence of eukaryote-prokaryote partnerships.</p> <p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p> kelp and their microbiome largerWorkCitation project eng; USA oceans -124.74557 -124.71266 48.38962 48.39518 1998-08-24 2025-08-23 Northeast Pacific Ocean, coastal Washington state 0 BCO-DMO catalogue of parameters from Dissolved nutrient data from samples collected from 1998 through 2025 from the surface waters of Tatoosh Island, Makah Reservation, Washington, USA Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/1004225.rdf Name: ISO_DateTime_Local Units: unitless Description: <p>Date and time in the local time zone (PDT) in ISO 8601 format</p> http://lod.bco-dmo.org/id/dataset-parameter/1004402.rdf Name: ISO_DateTime_UTC Units: unitless Description: <p>Date and time of sampling converted to UTC in ISO 8601 format</p> http://lod.bco-dmo.org/id/dataset-parameter/1004226.rdf Name: Date_local Units: unitless Description: <p>Date of sampling in the local time zone (PDT)</p> http://lod.bco-dmo.org/id/dataset-parameter/1004227.rdf Name: Time_local Units: unitless Description: <p>Time of sampling in the local time zone (PDT)</p> http://lod.bco-dmo.org/id/dataset-parameter/1004228.rdf Name: Site Units: unitless Description: <p>Name of sampling site</p> http://lod.bco-dmo.org/id/dataset-parameter/1004229.rdf Name: Latitude Units: Decimal degrees N Description: <p>Latitude of sampling site</p> http://lod.bco-dmo.org/id/dataset-parameter/1004231.rdf Name: Longitude Units: Decimal degrees W Description: <p>Longitude of sampling site</p> http://lod.bco-dmo.org/id/dataset-parameter/1004233.rdf Name: NO3 Units: micromolar (uM) Description: <p>Nitrate concentration</p> http://lod.bco-dmo.org/id/dataset-parameter/1004234.rdf Name: NO2 Units: micromolar (uM) Description: <p>Nitrite concentration</p> http://lod.bco-dmo.org/id/dataset-parameter/1004235.rdf Name: NH4 Units: micromolar (uM) Description: <p>Ammonium concentration</p> http://lod.bco-dmo.org/id/dataset-parameter/1004236.rdf Name: PO4 Units: micromolar (uM) Description: <p>Orthophosphate concentration</p> http://lod.bco-dmo.org/id/dataset-parameter/1004237.rdf Name: SiOH4 Units: micromolar (uM) Description: <p>Silicic Acid concentration</p> http://lod.bco-dmo.org/id/dataset-parameter/1004238.rdf Name: Lab Units: unitless Description: <p>Analysis laboratory: UW (University of Washington Marine Chemistry Lab), ML (Moss Landing Marine Lab Environmental Laboratory), MLtoUW (Analyzed at Moss Landing, converted to University of Washington measurements)</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact https://doi.org/10.26008/1912/bco-dmo.1004161.1 download onLine dataset &lt;p&gt;All censusing was done from shore or with small boats. Sampling stations were established on the shoreline of Tatoosh Island and adjacent offshore sites to the east of the island beginning in August 1998. Seven of the sites (Finger, Glacier Control, Hedophyllum Cove, Ladd's Finger, Simon's Landing, Strawberry Point, Toad Point) corresponded to wave-exposed shores on the periphery of the island used in a prior nutrient manipulation experiment (Wootton et al. 1996), and an eighth (Northwest Point) was another wave-exposed shore selected to expand peripheral coverage of the island. Two additional relatively wave-sheltered sites were also added to the sampling, both located inside extensive kelp beds: Main Beach and Strawberry Draw. Water at all these sites was collected 2-3 hours after a strong spring tide on a day when the investigators were departing from the island to facilitate rapid sample freezing. Water samples were collected in 2001, 2015, 2016, and 2022 at an additional shoreline site (Hydrolab Pool) subject to intensive sampling of ocean conditions. In 2000, four offshore sampling sites were established at varying distances from the island (Inside Kelp, ~30 meters (m); Outside Kelp, ~100 m; Gut, ~0.6 kilometers (km); Mushroom Rocks, ~ 1.8 km from island, ~0.5 km from the mainland). Water at the offshore sites was collected by boat approximately 4-5 hours after the tide. Water was extracted from the ocean at the surface using 50-milliliter (mL) syringes, which were first rinsed in the water at the site before a sample was drawn. The sample was then pushed through a syringe filter loaded with a disk of Whatman GF/F glass fiber filter paper, and collected in 40 mL acid-washed Nalgene bottles, leaving enough headspace to allow water expansion when frozen.&lt;/p&gt; &lt;p&gt;Water samples were kept in coolers following collection and immediately frozen at -20 degrees Celsius upon return to the mainland field base. Frozen water samples were express shipped to the University of Washington Ocean Chemistry Laboratory, where they were analyzed following the methods outlined in (UNESCO 1994) using a Seal Analytical AA3 nutrient autoanalyzer. In 2025, the analysis of samples from all dates/sites was carried out by the Moss Landing Marine Laboratory Environmental Analytical Laboratory, by colorimetric analysis using a Lachat QuickChem 8000 Flow Injection Analyzer and 50 mL Falcon Tubes as sample containers. We sent duplicate samples from 29 May 2025 and 23 August 2025 to the University of Washington Marine Chemistry Laboratory for comparative purposes.&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;No significant data processing, except in 2025, where relationships between University of Washington and Moss Landing labs were developed and data transformed following these relationships to make Moss Landing analyzed samples more equivalent to University of Washington samples for sample dates without University of Washington lab analysis. The relationships were derived from linear regression on log-transformed data of University of Washington nutrient data on Moss Landing nutrient data for each nutrient considered.&lt;/p&gt; Specified by the Principal Investigator(s) - Imported the original file &quot;Tatoosh Water Nutrients Long BCODMO.csv&quot; into the BCO-DMO data processing system. - Renamed column Si(OH)4 to SiOH4 to comply with BCO-DMO naming conventions. - Converted Date and Time columns (parsed as %Y-%m-%d and %H:%M respectively) into new datetime column ISO_DateTime_Local, formatted as %Y-%m-%dT%H:%M. - Converted Date and Time columns (local Pacific time, PST8PDT) into a new datetime column ISO_DateTime_UTC, converting to UTC and formatted as %Y-%m-%dT%H:%MZ. - Saved the final file as &quot;1004161_v1_tatoosh_island_nutrient_data.csv&quot;. Specified by BCO-DMO Data Managers asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact Lachat QuickChem 8000 Flow Injection Analyzer Lachat QuickChem 8000 Flow Injection Analyzer PI Supplied Instrument Name: Lachat QuickChem 8000 Flow Injection Analyzer PI Supplied Instrument Description:Analysis was carried out by the University of Washington Marine Chemistry Laboratory using a Seal Analytical AA3 nutrient autoanalyzer, and by the Moss Landing Marine Laboratory Environmental Analytical Laboratory using a Lachat QuickChem 8000 Flow Injection Analyzer. Instrument Name: Lachat QuikChem 8000 flow injection analyzer and Ion Chromatography (IC) system Instrument Short Name: Instrument Description: The Lachat QuikChem 8000 can operate flow injection analysis and ion chromatography simultaneously and independently on the same instrument platform. Instrument includes sampler, dilutor, sampling pump, electronics unit, and data station. Analysis takes 20-60 seconds, with a sample throughput of 60-120 samples per hour. Measurements are in the range of parts per trillion to parts per hundred. Seal Analytical AA3 nutrient autoanalyzer Seal Analytical AA3 nutrient autoanalyzer PI Supplied Instrument Name: Seal Analytical AA3 nutrient autoanalyzer PI Supplied Instrument Description:Analysis was carried out by the University of Washington Marine Chemistry Laboratory using a Seal Analytical AA3 nutrient autoanalyzer, and by the Moss Landing Marine Laboratory Environmental Analytical Laboratory using a Lachat QuickChem 8000 Flow Injection Analyzer. Instrument Name: Seal Analytical AutoAnalyser 3HR Instrument Short Name:Seal Analytical AutoAnalyser 3HR Instrument Description: A fully automated Segmented Flow Analysis (SFA) system, ideal for water and seawater analysis. It comprises a modular system which integrates an autosampler, peristaltic pump, chemistry manifold and detector. The sample and reagents are pumped continuously through the chemistry manifold, and air bubbles are introduced at regular intervals forming reaction segments which are mixed using glass coils. The AA3 uses segmented flow analysis principles to reduce inter-sample dispersion, and can analyse up to 100 samples per hour using stable LED light sources.