http://lod.bco-dmo.org/id/dataset/1004302 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2026-08-10 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) DOM analysis by LCMS for submarine groundwater and river samples collected between November 2022 and September 2023 along the West Florida Shelf 2026-08-20 publication 2026-08-20 revision Woods Hole Oceanographic Institution (WHOI BCO-DMO) 2026-08-20 publication https://doi.org/10.26008/1912/bco-dmo.1004302.1 Rene Maurice Boiteau Oregon State University principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Boiteau, R. M., Huang, C., Farrell, I. (2026) DOM analysis by LCMS for submarine groundwater and river samples collected between November 2022 and September 2023 along the West Florida Shelf. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-08-20 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.1004302.1 [access date] STING SGD &amp; River - LCMS DOM Dataset Description: Methods and Sampling: &lt;p&gt;&lt;strong&gt;Sample collection and solid phase extraction:&lt;/strong&gt;&lt;br /&gt; Surface water samples (500 milliliters (mL)) were collected at all river stations via a submersible pump lowered ~1.0 meter (m) below the water's surface. For the collection of submarine groundwater samples, U.S. Geological Survey small boat operations along the West Florida Shelf (WFS) were conducted. Surface water, bottom water, and submarine groundwater samples (2 liters (L)) were collected via peristaltic pump along three coastal transects.&lt;/p&gt; &lt;p&gt;Samples were filtered through 0.2 micrometer (um) filters (polyethersulfone) into acid-cleaned polypropylene bottles to 2L. The sample was then acidified to a pH of 2-3 with hydrochloric acid (trace metal grade) and pumped at 25 milliliters per minute (mL/min) through Bond-Elut PPL solid phase extraction (SPE) columns (1 gram (g), 6 mL, Agilent Technologies) that had been previously activated by passing 6 mL each of methanol (MeOH, Optima LCMS grade, Fisher Scientific) and ultrapure water (qH₂O, 18.2 MΩ) through the column. SPE columns were rinsed with 10 mL acidified qH₂O (pH = 2) to remove salts and frozen at -20 degrees Celsius (°C) immediately after sample collection and returned to the laboratory for further processing. Sample columns were gravity eluted with 10 mL LCMS Optima grade methanol. The eluent was collected in 15 mL polypropylene centrifuge tube and evaporated in a SpeedVac to concentrate to a volume of ~ 0.2 mL. The eluent was transferred to a 2 mL centrifuge tube and brought up to a final volume of 2 mL with qH₂O. Each sample was spiked with 20 microliters (µL) of 100 micromolar (µM) B12 stock for a concentration of 1 µM cyanocobalamin. Solid phase extraction process blank samples were prepared with the same procedure without sample loading.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Analysis by LCMS:&lt;/strong&gt;&lt;br /&gt; Tubes were centrifuged at max speed for 5 minutes to sediment any particles, and then the spiked samples were transferred to plastic microcentrifuge tubes for storage in freezer, 1 mL was used for analysis on Orbitrap in 2 mL plastic autosampler vials. A pooled sample from each batch of samples for each Orbitrap run was prepared by combining 15 µL from each sample. Chromatographic separation was performed using a Phenyl-Hexyl column (Waters ACQUITY Premier CSH) with dimensions of 2.1 x 100 millimeters (mm) and a 1.7 µm particle size. The column temperature was maintained at 30.0 °C throughout the analytical run. Mobile phase A was composed of water supplemented with 0.1% formic acid, while mobile phase B consisted of methanol with 0.1% formic acid. The system operated at a constant flow rate of 200 µL/min with a 30-minute gradient starting from 95% solvent A and 5% solvent B to 5% solvent A and 95% solvent B and a 5-minute hold at 95% solvent B. Solvents were then switched back to the initial condition of 95% solvent A and held for 6 minutes to re-equilibrate the column. The total injection volume for each sample was 20 µL. Mass spectral data were acquired using an Orbitrap IQ-X Tribrid Mass Spectrometer (Thermo Fisher Scientific) operating in positive electrospray ionization (ESI) mode. The ESI source parameters were optimized with a spray voltage of 3500 volts (V), a sheath gas flow of 50 (Arb), an auxiliary gas flow of 10 (Arb), and a sweep gas flow of 1 (Arb). The ion transfer tube and vaporizer temperatures were set to 325 °C and 350 °C, respectively. Full MS scans were recorded in the Orbitrap mass analyzer across a mass range of 100 to 1000 m/z. The resolution was set to 500,000 at m/z 200 to ensure high mass accuracy for the analytes. Data acquisition settings included the collection of 1 microscan per data point with a maximum injection time of 1014 milliseconds (ms).&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2422709 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2422709 completed Rene Maurice Boiteau Oregon State University 612-625-6983 228 Smith Hall 207 Pleasant Street Se Minneapolis MN 55455 USA rboiteau@umn.edu pointOfContact asNeeded Dataset Version: 1 Unknown Time Molecular_Formula Calculated_m_z m_z m_z_Error_ppm Calibrated_m_z Intensity_CH_250617_SGDRIVER_20250617_pooled_117 Intensity_CH_250617_SGDRIVER_20250617_pooled_117_n56 Intensity_CH_250617_SGDRIVER_20250617_pooled_3_n3 Intensity_CH_250617_SGDRIVER_20250617_pooled_66_n13 Intensity_CH_250617_SGDRIVER_AR1_AUG_38_n40 Intensity_CH_250617_SGDRIVER_AR1_MAYJUNE_102 Intensity_CH_250617_SGDRIVER_NOV_VH55BW_14_n14 Intensity_CH_250617_SGDRIVER_OFS17BW_JUNE_57 Intensity_CH_250617_SGDRIVER_PR1_02092023_39_n41 Intensity_CH_250617_SGDRIVER_VH45BW_JUNE_101 Intensity_CH_250617_SGDRIVER_VH50GD_02082023_114 Intensity_CH_250617_SGDRIVER_VH55BW_02082023_106 Intensity_CH_250617_SGDRIVER_HB1_AUG_71 Intensity_CH_250617_SGDRIVER_HB1_MAYJUNE_42_n44 Intensity_CH_250617_SGDRIVER_IRB30BW_01302023_56 Intensity_CH_250617_SGDRIVER_IRB30BW_JUNE_54 Intensity_CH_250617_SGDRIVER_IRB30BW_SEPT_81 Intensity_CH_250617_SGDRIVER_MR1_AUG_83 Intensity_CH_250617_SGDRIVER_MR1_MAYJUNE_92 Intensity_CH_250617_SGDRIVER_VH55GD_JUNE_69 Intensity_CH_250617_SGDRIVER_VH55GD_SEPT_76 Intensity_CH_250617_SGDRIVER_NC10GD_SEPT_46_n48 Intensity_CH_250617_SGDRIVER_NC15BW_02022023_90 Intensity_CH_250617_SGDRIVER_NC15BW_JUNE_77 Intensity_CH_250617_SGDRIVER_NOV_IRB25BW_59 Intensity_CH_250617_SGDRIVER_NOV_IRB25GD_64 Intensity_CH_250617_SGDRIVER_NOV_VH45BW_20_n20 Intensity_CH_250617_SGDRIVER_NOV_VH50GD_7_n6 Intensity_CH_250617_SGDRIVER_OFS36BW_JUNE_82 Intensity_CH_250617_SGDRIVER_OFS38BW_JUNE_91 Intensity_CH_250617_SGDRIVER_OFS44BW_JUNE_25_25 Intensity_CH_250617_SGDRIVER_VH50BW_JUNE_34_n34 Intensity_CH_250617_SGDRIVER_VH50BW_SEPT_41_n43 Intensity_CH_250617_SGDRIVER_VH50GD_JUNE_22_n22 Intensity_CH_250617_SGDRIVER_VH50GD_SEPT_44_n46 Intensity_CH_250617_SGDRIVER_VH55BW_JUNE_80 Intensity_CH_250617_SGDRIVER_VH55BW_SEPT_16_n16 Intensity_CH_250617_SGDRIVER_IRB35BW_01302023_21_n21 Intensity_CH_250617_SGDRIVER_IRB35BW_SEPT_19_n19 Intensity_CH_250617_SGDRIVER_IRB35GD_JUNE_89_n36 Intensity_CH_250617_SGDRIVER_IRB35GD_SEPT_110 Intensity_CH_250617_SGDRIVER_AR2_MAYJUNE_94 Intensity_CH_250617_SGDRIVER_Blank2_SEPT_9_n8 Intensity_CH_250617_SGDRIVER_ConwayBlank_02022023_23_n23 Intensity_CH_250617_SGDRIVER_ConwayBlank_020922023_95 Intensity_CH_250617_SGDRIVER_NC15GD_JUNE_86 Intensity_CH_250617_SGDRIVER_NC15GD_SEPT_68 Intensity_CH_250617_SGDRIVER_NOV_Blank231122_0513_33_n33 Intensity_CH_250617_SGDRIVER_OFS01BW_JUNE_28_n28 Intensity_CH_250617_SGDRIVER_OFS13BW_JUNE_65 Intensity_CH_250617_SGDRIVER_20250617_pooled_24_n24 Intensity_CH_250617_SGDRIVER_20250617_pooled_66_n35 Intensity_CH_250617_SGDRIVER_AR2_AUG_30_n30 Intensity_CH_250617_SGDRIVER_Blank_SEPT_60 Intensity_CH_250617_SGDRIVER_CR1_02092023_78 Intensity_CH_250617_SGDRIVER_HB1_02062023_88 Intensity_CH_250617_SGDRIVER_HB2_02062023_36_n38 Intensity_CH_250617_SGDRIVER_IRB25BW_JUNE_35_n37 Intensity_CH_250617_SGDRIVER_IRB30GD_02012023_100 Intensity_CH_250617_SGDRIVER_IRB30GD_SEPT_12_n11 Intensity_CH_250617_SGDRIVER_IRB35BW_JUNE_84 Intensity_CH_250617_SGDRIVER_IRB35GD_020122023_98 Intensity_CH_250617_SGDRIVER_CR1_AUG_109 Intensity_CH_250617_SGDRIVER_CR2_02082023_107 Intensity_CH_250617_SGDRIVER_MR2_02062023_11_n10 Intensity_CH_250617_SGDRIVER_HB2_AUG_111 Intensity_CH_250617_SGDRIVER_HB2_MAYJUNE_99 Intensity_CH_250617_SGDRIVER_IRB25BW_SEPT_112 Intensity_CH_250617_SGDRIVER_IRB25GD_02012023_79 Intensity_CH_250617_SGDRIVER_IRB25GD_JUNE_5_n4 Intensity_CH_250617_SGDRIVER_IRB25GD_SEPT_116 Intensity_CH_250617_SGDRIVER_OFS18BW_JUNE_18_n18 Intensity_CH_250617_SGDRIVER_OFS31BW_JUNE_113 Intensity_CH_250617_SGDRIVER_OFS32BW_INC_JUNE_73 Intensity_CH_250617_SGDRIVER_OFS32BW_JUNE_96 Intensity_CH_250617_SGDRIVER_OFS35BW_JUNE_47_n49 Intensity_CH_250617_SGDRIVER_SPEBlank_08012023_75 Intensity_CH_250617_SGDRIVER_VH45BW_02092023_10_n9 Intensity_CH_250617_SGDRIVER_NOV_NC10BW_105 Intensity_CH_250617_SGDRIVER_NOV_NC10GD_61 Intensity_CH_250617_SGDRIVER_NOV_NC15BW_40_n42 Intensity_CH_250617_SGDRIVER_NOV_NC15GD_50_n52 Intensity_CH_250617_SGDRIVER_NOV_IRB30GD_17_n17 Intensity_CH_250617_SGDRIVER_NOV_IRB35GD_27_n27 Intensity_CH_250617_SGDRIVER_NOV_MR1_67 Intensity_CH_250617_SGDRIVER_NOV_MR2_53 Intensity_CH_250617_SGDRIVER_NOV_NC05BW_8_n7 Intensity_CH_250617_SGDRIVER_MR2_AUG_37_n39 Intensity_CH_250617_SGDRIVER_MR2_MAYJUNE_51_n53 Intensity_CH_250617_SGDRIVER_NC05BW_SEPT_31_n31 Intensity_CH_250617_SGDRIVER_NC05GD_JUNE_48_n50 Intensity_CH_250617_SGDRIVER_NC05GD_SEPT_6_n5 Intensity_CH_250617_SGDRIVER_NC10BW_SEPT_55 Intensity_CH_250617_SGDRIVER_NC10GD_02022023_103 Intensity_CH_250617_SGDRIVER_NC10GD_JUNE_58 Intensity_CH_250617_SGDRIVER_062723Blank_JUNE_52_n54 Intensity_CH_250617_SGDRIVER_20250617_pooled_108 Intensity_CH_250617_SGDRIVER_20250617_pooled_87 Intensity_CH_250617_SGDRIVER_AR1_02062023_43_n45 Intensity_CH_250617_SGDRIVER_NOV_HB1_0513_32_n32 Intensity_CH_250617_SGDRIVER_NOV_HB2_85 Intensity_CH_250617_SGDRIVER_VH45BW_SEPT_93 Intensity_CH_250617_SGDRIVER_VH50BW_02082023_49_n51 Intensity_CH_250617_SGDRIVER_IRB30GD_JUNE_62 Intensity_CH_250617_SGDRIVER_NC15GD_02022023_72 Intensity_CH_250617_SGDRIVER_NC15BW_SEPT_104 Intensity_CH_250617_SGDRIVER_NOV_AR1_97 Intensity_CH_250617_SGDRIVER_NOV_Blank291122_26_n26 Intensity_CH_250617_SGDRIVER_NOV_IRB30BW_15_n15 Intensity_CH_250617_SGDRIVER_PR1_AUG_13_n12 Stoichiometric_classification O_C H_C N_C centrifuge Thermo Scientific Orbitrap IQ-X Mass Spectrometer submersible pump peristaltic pump Vanquish Horizon liquid chromatography system theme None, User defined time_point chemical_formula mass-to-charge ratio (m/z) Intensity (relative ion abundance) sample description ratio featureType BCO-DMO Standard Parameters Centrifuge Mass Spectrometer Pump Pump Ultra-high-performance liquid chromatography instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Collaborative Research: Linking iron and nitrogen sources in an oligotrophic coastal margin: Nitrogen fixation and the role of boundary fluxes https://osprey.bco-dmo.org/project/880516 Collaborative Research: Linking iron and nitrogen sources in an oligotrophic coastal margin: Nitrogen fixation and the role of boundary fluxes <p><strong>NSF Award Abstract:</strong><br /> This project will investigate how groundwater discharge delivers important nutrients to the coastal ecosystems of the West Florida Shelf. Preliminary studies indicate that groundwater may supply both dissolved organic nitrogen (DON) and iron in this region. In coastal ecosystems like the West Florida Shelf that have very low nitrate and ammonium concentrations, DON is the main form of nitrogen available to organisms. Nitrogen cycling is strongly affected by iron availability because iron is essential for both photosynthesis and for nitrogen fixation. This study will investigate the sources and composition of DON and iron, and their influence on the coastal ecosystem. The team will sample offshore groundwater wells, river and estuarine waters, and conduct two expeditions across the West Florida Shelf in winter and summer. Investigators will participate in K-12 and outreach activities to increase awareness of the project and related science. The project will fund the work of six graduate and eight undergraduate students across five institutions, furthering NSF’s goals of education and training.</p> <p>Motivated by preliminary observations of unexplained, tightly-correlated DON and dissolved iron concentrations across the West Florida Shelf (WFS), the proposed work will quantify the flux and isotopic signatures of submarine groundwater discharge (SGD)-derived DON and iron to the WFS, and evaluate the bioavailability of this temporally-variable source using four seasonal near-shore campaigns sampling offshore groundwater wells, estuarine, and riverine endmembers and two cross-shelf cruises. The work will evaluate whether SGD stimulates nitrogen fixation on the WFS, and the potential for the stimulated nitrogen fixation to further modify the chemistry of DON and dissolved iron in the region. The cross-shelf cruises will investigate hypothesized periods of maximum SGD and Trichodesmium abundance (June), and reduced river discharge and SGD (February), thus comparing two distinct biogeochemical regimes. The concentrations and isotopic compositions of DON and dissolved iron, molecular composition of DON, and the concentration and composition of iron-binding ligands will be characterized. Nitrogen fixation rates and Trichodesmium spp. abundance and expression of iron stress genes will be measured. Fluxes of DON and iron from SGD and rivers will be quantified with radium isotope mass balances. The impacts of SGD on nitrogen fixation and DON/ligand production will be constrained with incubations of natural phytoplankton communities with submarine groundwater amendments. Two hypotheses will be tested: 1) SGD is the dominant source of bioavailable DON and dissolved iron on the WFS, and 2) SGD-alleviation of iron stress changes the dominant Trichodesmium species on the WFS, increases nitrogen fixation rates and modifies DON and iron composition. Overall, the work will establish connections between marine nitrogen and iron cycling and evaluate the potential for coastal inputs to modify water along the WFS before export to the Atlantic Ocean. This study will thus provide a framework to consider these boundary fluxes in oligotrophic coastal systems and the relative importance of rivers and SGD as sources of nitrogen and iron in other analogous locations, such as coastal systems in Australia, India, and Africa, where nitrogen fixation and SGD have also been documented.</p> <p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p> West Florida Shelf DON and Fe largerWorkCitation project eng; USA oceans -83.0012 -81.8474 26.5321 28.7669 2022-11-14 2023-09-14 Gulf of Mexico/America, West Florida Shelf 0 BCO-DMO catalogue of parameters from DOM analysis by LCMS for submarine groundwater and river samples collected between November 2022 and September 2023 along the West Florida Shelf Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/1004518.rdf Name: Time Units: minutes Description: <p>the time interval that the chromotographic retention time of this feature belongs to</p> http://lod.bco-dmo.org/id/dataset-parameter/1004519.rdf Name: Molecular_Formula Units: unitless Description: <p>The molecular formula assigned to the feature</p> http://lod.bco-dmo.org/id/dataset-parameter/1004520.rdf Name: Calculated_m_z Units: m/z Description: <p>mass to charge ratio (m/z) calculated from the assigned molecular formula</p> http://lod.bco-dmo.org/id/dataset-parameter/1004521.rdf Name: m_z Units: m/z Description: <p>detected mass to charge ratio</p> http://lod.bco-dmo.org/id/dataset-parameter/1004522.rdf Name: m_z_Error_ppm Units: m/z Description: <p>the difference between calculated m/z and calibrated m/z</p> http://lod.bco-dmo.org/id/dataset-parameter/1004523.rdf Name: Calibrated_m_z Units: m/z Description: <p>calibrated m/z</p> http://lod.bco-dmo.org/id/dataset-parameter/1004524.rdf Name: Intensity_CH_250617_SGDRIVER_20250617_pooled_117 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004525.rdf Name: Intensity_CH_250617_SGDRIVER_20250617_pooled_117_n56 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004526.rdf Name: Intensity_CH_250617_SGDRIVER_20250617_pooled_3_n3 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004527.rdf Name: Intensity_CH_250617_SGDRIVER_20250617_pooled_66_n13 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004528.rdf Name: Intensity_CH_250617_SGDRIVER_AR1_AUG_38_n40 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004529.rdf Name: Intensity_CH_250617_SGDRIVER_AR1_MAYJUNE_102 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004530.rdf Name: Intensity_CH_250617_SGDRIVER_NOV_VH55BW_14_n14 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004531.rdf Name: Intensity_CH_250617_SGDRIVER_OFS17BW_JUNE_57 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004532.rdf Name: Intensity_CH_250617_SGDRIVER_PR1_02092023_39_n41 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004533.rdf Name: Intensity_CH_250617_SGDRIVER_VH45BW_JUNE_101 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004534.rdf Name: Intensity_CH_250617_SGDRIVER_VH50GD_02082023_114 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004535.rdf Name: Intensity_CH_250617_SGDRIVER_VH55BW_02082023_106 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004536.rdf Name: Intensity_CH_250617_SGDRIVER_HB1_AUG_71 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004537.rdf Name: Intensity_CH_250617_SGDRIVER_HB1_MAYJUNE_42_n44 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004538.rdf Name: Intensity_CH_250617_SGDRIVER_IRB30BW_01302023_56 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004539.rdf Name: Intensity_CH_250617_SGDRIVER_IRB30BW_JUNE_54 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004540.rdf Name: Intensity_CH_250617_SGDRIVER_IRB30BW_SEPT_81 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004541.rdf Name: Intensity_CH_250617_SGDRIVER_MR1_AUG_83 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004542.rdf Name: Intensity_CH_250617_SGDRIVER_MR1_MAYJUNE_92 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004543.rdf Name: Intensity_CH_250617_SGDRIVER_VH55GD_JUNE_69 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004544.rdf Name: Intensity_CH_250617_SGDRIVER_VH55GD_SEPT_76 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004545.rdf Name: Intensity_CH_250617_SGDRIVER_NC10GD_SEPT_46_n48 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004546.rdf Name: Intensity_CH_250617_SGDRIVER_NC15BW_02022023_90 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004547.rdf Name: Intensity_CH_250617_SGDRIVER_NC15BW_JUNE_77 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004548.rdf Name: Intensity_CH_250617_SGDRIVER_NOV_IRB25BW_59 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004549.rdf Name: Intensity_CH_250617_SGDRIVER_NOV_IRB25GD_64 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004550.rdf Name: Intensity_CH_250617_SGDRIVER_NOV_VH45BW_20_n20 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004551.rdf Name: Intensity_CH_250617_SGDRIVER_NOV_VH50GD_7_n6 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004552.rdf Name: Intensity_CH_250617_SGDRIVER_OFS36BW_JUNE_82 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004553.rdf Name: Intensity_CH_250617_SGDRIVER_OFS38BW_JUNE_91 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004554.rdf Name: Intensity_CH_250617_SGDRIVER_OFS44BW_JUNE_25_25 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004555.rdf Name: Intensity_CH_250617_SGDRIVER_VH50BW_JUNE_34_n34 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004556.rdf Name: Intensity_CH_250617_SGDRIVER_VH50BW_SEPT_41_n43 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004557.rdf Name: Intensity_CH_250617_SGDRIVER_VH50GD_JUNE_22_n22 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004558.rdf Name: Intensity_CH_250617_SGDRIVER_VH50GD_SEPT_44_n46 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004559.rdf Name: Intensity_CH_250617_SGDRIVER_VH55BW_JUNE_80 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004560.rdf Name: Intensity_CH_250617_SGDRIVER_VH55BW_SEPT_16_n16 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004561.rdf Name: Intensity_CH_250617_SGDRIVER_IRB35BW_01302023_21_n21 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004562.rdf Name: Intensity_CH_250617_SGDRIVER_IRB35BW_SEPT_19_n19 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004563.rdf Name: Intensity_CH_250617_SGDRIVER_IRB35GD_JUNE_89_n36 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004564.rdf Name: Intensity_CH_250617_SGDRIVER_IRB35GD_SEPT_110 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004565.rdf Name: Intensity_CH_250617_SGDRIVER_AR2_MAYJUNE_94 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004566.rdf Name: Intensity_CH_250617_SGDRIVER_Blank2_SEPT_9_n8 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004567.rdf Name: Intensity_CH_250617_SGDRIVER_ConwayBlank_02022023_23_n23 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004568.rdf Name: Intensity_CH_250617_SGDRIVER_ConwayBlank_020922023_95 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004569.rdf Name: Intensity_CH_250617_SGDRIVER_NC15GD_JUNE_86 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004570.rdf Name: Intensity_CH_250617_SGDRIVER_NC15GD_SEPT_68 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004571.rdf Name: Intensity_CH_250617_SGDRIVER_NOV_Blank231122_0513_33_n33 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004572.rdf Name: Intensity_CH_250617_SGDRIVER_OFS01BW_JUNE_28_n28 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004573.rdf Name: Intensity_CH_250617_SGDRIVER_OFS13BW_JUNE_65 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004574.rdf Name: Intensity_CH_250617_SGDRIVER_20250617_pooled_24_n24 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004575.rdf Name: Intensity_CH_250617_SGDRIVER_20250617_pooled_66_n35 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004576.rdf Name: Intensity_CH_250617_SGDRIVER_AR2_AUG_30_n30 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004577.rdf Name: Intensity_CH_250617_SGDRIVER_Blank_SEPT_60 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004578.rdf Name: Intensity_CH_250617_SGDRIVER_CR1_02092023_78 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004579.rdf Name: Intensity_CH_250617_SGDRIVER_HB1_02062023_88 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004580.rdf Name: Intensity_CH_250617_SGDRIVER_HB2_02062023_36_n38 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004581.rdf Name: Intensity_CH_250617_SGDRIVER_IRB25BW_JUNE_35_n37 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004582.rdf Name: Intensity_CH_250617_SGDRIVER_IRB30GD_02012023_100 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004583.rdf Name: Intensity_CH_250617_SGDRIVER_IRB30GD_SEPT_12_n11 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004584.rdf Name: Intensity_CH_250617_SGDRIVER_IRB35BW_JUNE_84 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004585.rdf Name: Intensity_CH_250617_SGDRIVER_IRB35GD_020122023_98 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004586.rdf Name: Intensity_CH_250617_SGDRIVER_CR1_AUG_109 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004587.rdf Name: Intensity_CH_250617_SGDRIVER_CR2_02082023_107 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004588.rdf Name: Intensity_CH_250617_SGDRIVER_MR2_02062023_11_n10 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004589.rdf Name: Intensity_CH_250617_SGDRIVER_HB2_AUG_111 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004590.rdf Name: Intensity_CH_250617_SGDRIVER_HB2_MAYJUNE_99 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004591.rdf Name: Intensity_CH_250617_SGDRIVER_IRB25BW_SEPT_112 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004592.rdf Name: Intensity_CH_250617_SGDRIVER_IRB25GD_02012023_79 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004593.rdf Name: Intensity_CH_250617_SGDRIVER_IRB25GD_JUNE_5_n4 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004594.rdf Name: Intensity_CH_250617_SGDRIVER_IRB25GD_SEPT_116 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004595.rdf Name: Intensity_CH_250617_SGDRIVER_OFS18BW_JUNE_18_n18 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004596.rdf Name: Intensity_CH_250617_SGDRIVER_OFS31BW_JUNE_113 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004597.rdf Name: Intensity_CH_250617_SGDRIVER_OFS32BW_INC_JUNE_73 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004598.rdf Name: Intensity_CH_250617_SGDRIVER_OFS32BW_JUNE_96 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004599.rdf Name: Intensity_CH_250617_SGDRIVER_OFS35BW_JUNE_47_n49 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004600.rdf Name: Intensity_CH_250617_SGDRIVER_SPEBlank_08012023_75 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004601.rdf Name: Intensity_CH_250617_SGDRIVER_VH45BW_02092023_10_n9 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004602.rdf Name: Intensity_CH_250617_SGDRIVER_NOV_NC10BW_105 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004603.rdf Name: Intensity_CH_250617_SGDRIVER_NOV_NC10GD_61 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004604.rdf Name: Intensity_CH_250617_SGDRIVER_NOV_NC15BW_40_n42 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004605.rdf Name: Intensity_CH_250617_SGDRIVER_NOV_NC15GD_50_n52 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004606.rdf Name: Intensity_CH_250617_SGDRIVER_NOV_IRB30GD_17_n17 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004607.rdf Name: Intensity_CH_250617_SGDRIVER_NOV_IRB35GD_27_n27 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004608.rdf Name: Intensity_CH_250617_SGDRIVER_NOV_MR1_67 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004609.rdf Name: Intensity_CH_250617_SGDRIVER_NOV_MR2_53 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004610.rdf Name: Intensity_CH_250617_SGDRIVER_NOV_NC05BW_8_n7 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004611.rdf Name: Intensity_CH_250617_SGDRIVER_MR2_AUG_37_n39 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004612.rdf Name: Intensity_CH_250617_SGDRIVER_MR2_MAYJUNE_51_n53 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004613.rdf Name: Intensity_CH_250617_SGDRIVER_NC05BW_SEPT_31_n31 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004614.rdf Name: Intensity_CH_250617_SGDRIVER_NC05GD_JUNE_48_n50 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004615.rdf Name: Intensity_CH_250617_SGDRIVER_NC05GD_SEPT_6_n5 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004616.rdf Name: Intensity_CH_250617_SGDRIVER_NC10BW_SEPT_55 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004617.rdf Name: Intensity_CH_250617_SGDRIVER_NC10GD_02022023_103 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004618.rdf Name: Intensity_CH_250617_SGDRIVER_NC10GD_JUNE_58 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004619.rdf Name: Intensity_CH_250617_SGDRIVER_062723Blank_JUNE_52_n54 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004620.rdf Name: Intensity_CH_250617_SGDRIVER_20250617_pooled_108 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004621.rdf Name: Intensity_CH_250617_SGDRIVER_20250617_pooled_87 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004622.rdf Name: Intensity_CH_250617_SGDRIVER_AR1_02062023_43_n45 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004623.rdf Name: Intensity_CH_250617_SGDRIVER_NOV_HB1_0513_32_n32 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004624.rdf Name: Intensity_CH_250617_SGDRIVER_NOV_HB2_85 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004625.rdf Name: Intensity_CH_250617_SGDRIVER_VH45BW_SEPT_93 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004626.rdf Name: Intensity_CH_250617_SGDRIVER_VH50BW_02082023_49_n51 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004627.rdf Name: Intensity_CH_250617_SGDRIVER_IRB30GD_JUNE_62 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004628.rdf Name: Intensity_CH_250617_SGDRIVER_NC15GD_02022023_72 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004629.rdf Name: Intensity_CH_250617_SGDRIVER_NC15BW_SEPT_104 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004630.rdf Name: Intensity_CH_250617_SGDRIVER_NOV_AR1_97 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004631.rdf Name: Intensity_CH_250617_SGDRIVER_NOV_Blank291122_26_n26 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004632.rdf Name: Intensity_CH_250617_SGDRIVER_NOV_IRB30BW_15_n15 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004633.rdf Name: Intensity_CH_250617_SGDRIVER_PR1_AUG_13_n12 Units: counts per second (CPS) Description: <p>Peak height intensity of the feature in this sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1004634.rdf Name: Stoichiometric_classification Units: unitless Description: <p>stoichiometric classification assigned to the feature based on molecular formula. These classifications are based on Rivas-Ubach et al. ‘Moving beyond the van Krevelen Diagram: A New Stoichiometric Approach for Compound Classification in Organisms‘ <a href="https://pubs.acs.org/doi/10.1021/acs.analchem.8b00529">https://pubs.acs.org/doi/10.1021/acs.analchem.8b00529</a></p> http://lod.bco-dmo.org/id/dataset-parameter/1004635.rdf Name: O_C Units: unitless Description: <p>elemental ratios (Oxygen/Carbon) in the molecular formula</p> http://lod.bco-dmo.org/id/dataset-parameter/1004636.rdf Name: H_C Units: unitless Description: <p>elemental ratios (Hydrogen/Carbon) in the molecular formula</p> http://lod.bco-dmo.org/id/dataset-parameter/1004637.rdf Name: N_C Units: unitless Description: <p>elemental ratios (Nitrogen/Carbon) in the molecular formula</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact https://doi.org/10.26008/1912/bco-dmo.1004302.1 download onLine dataset &lt;p&gt;&lt;strong&gt;Sample collection and solid phase extraction:&lt;/strong&gt;&lt;br /&gt; Surface water samples (500 milliliters (mL)) were collected at all river stations via a submersible pump lowered ~1.0 meter (m) below the water's surface. For the collection of submarine groundwater samples, U.S. Geological Survey small boat operations along the West Florida Shelf (WFS) were conducted. Surface water, bottom water, and submarine groundwater samples (2 liters (L)) were collected via peristaltic pump along three coastal transects.&lt;/p&gt; &lt;p&gt;Samples were filtered through 0.2 micrometer (um) filters (polyethersulfone) into acid-cleaned polypropylene bottles to 2L. The sample was then acidified to a pH of 2-3 with hydrochloric acid (trace metal grade) and pumped at 25 milliliters per minute (mL/min) through Bond-Elut PPL solid phase extraction (SPE) columns (1 gram (g), 6 mL, Agilent Technologies) that had been previously activated by passing 6 mL each of methanol (MeOH, Optima LCMS grade, Fisher Scientific) and ultrapure water (qH₂O, 18.2 MΩ) through the column. SPE columns were rinsed with 10 mL acidified qH₂O (pH = 2) to remove salts and frozen at -20 degrees Celsius (°C) immediately after sample collection and returned to the laboratory for further processing. Sample columns were gravity eluted with 10 mL LCMS Optima grade methanol. The eluent was collected in 15 mL polypropylene centrifuge tube and evaporated in a SpeedVac to concentrate to a volume of ~ 0.2 mL. The eluent was transferred to a 2 mL centrifuge tube and brought up to a final volume of 2 mL with qH₂O. Each sample was spiked with 20 microliters (µL) of 100 micromolar (µM) B12 stock for a concentration of 1 µM cyanocobalamin. Solid phase extraction process blank samples were prepared with the same procedure without sample loading.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Analysis by LCMS:&lt;/strong&gt;&lt;br /&gt; Tubes were centrifuged at max speed for 5 minutes to sediment any particles, and then the spiked samples were transferred to plastic microcentrifuge tubes for storage in freezer, 1 mL was used for analysis on Orbitrap in 2 mL plastic autosampler vials. A pooled sample from each batch of samples for each Orbitrap run was prepared by combining 15 µL from each sample. Chromatographic separation was performed using a Phenyl-Hexyl column (Waters ACQUITY Premier CSH) with dimensions of 2.1 x 100 millimeters (mm) and a 1.7 µm particle size. The column temperature was maintained at 30.0 °C throughout the analytical run. Mobile phase A was composed of water supplemented with 0.1% formic acid, while mobile phase B consisted of methanol with 0.1% formic acid. The system operated at a constant flow rate of 200 µL/min with a 30-minute gradient starting from 95% solvent A and 5% solvent B to 5% solvent A and 95% solvent B and a 5-minute hold at 95% solvent B. Solvents were then switched back to the initial condition of 95% solvent A and held for 6 minutes to re-equilibrate the column. The total injection volume for each sample was 20 µL. Mass spectral data were acquired using an Orbitrap IQ-X Tribrid Mass Spectrometer (Thermo Fisher Scientific) operating in positive electrospray ionization (ESI) mode. The ESI source parameters were optimized with a spray voltage of 3500 volts (V), a sheath gas flow of 50 (Arb), an auxiliary gas flow of 10 (Arb), and a sweep gas flow of 1 (Arb). The ion transfer tube and vaporizer temperatures were set to 325 °C and 350 °C, respectively. Full MS scans were recorded in the Orbitrap mass analyzer across a mass range of 100 to 1000 m/z. The resolution was set to 500,000 at m/z 200 to ensure high mass accuracy for the analytes. Data acquisition settings included the collection of 1 microscan per data point with a maximum injection time of 1014 milliseconds (ms).&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;All data were quality controlled and processed using CoreMS with inhouse python based scripts (&lt;a href=&quot;http://dx.doi.org/10.5281/zenodo.19744141&quot; target=&quot;_blank&quot;&gt;10.5281/zenodo.19744141&lt;/a&gt;). Open source data files were generated using MS Convert. These data files are publicly available in MassIVE under accession #MSV000101377. Orbitrap analyses included sample analyses that are tabulated in the sample list as well as solid phase extraction process blanks analyses (denoted with 'blank' in the file name), and pooled sample quality control analyses (denoted with 'pooled' in the file name).&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Quality control:&lt;/strong&gt;&lt;br /&gt; First, quality control was conducted by checking the extracted ion chromatography (EIC) of internal cyanocobalamin standard (m/z = 678.2915 for the doubly charged form of cyanocobalamin, with a m/z tolerance of 15 parts per million (ppm)) for each sample. Peak areas of internal standard across a time range of 10.5 to 12.0 minutes were calculated. Samples with internal standard peak area differing by more than two standard deviations from the mean across the data set were excluded from further analysis.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Molecular formula assignment:&lt;/strong&gt;&lt;br /&gt; To assign molecular formula, mass spectra was averaged in 2-minute intervals over a range of 0-30 minutes and a mass range between 100-580 m/z. Each spectra was internally calibrated based on a polynomial correction applied to peaks within a CHON series reference list that included calibrant mass peaks across the entire m/z and retention time range. Search criteria for molecular formula assignments included: C 1-40, H 4-80, O 0-16, N 0-8, S 0-1, and Na 0-1, with a maximum allowed double bond equivalent (DBE = C – (H/2) + (N/2) + 1) of 16 and a maximum mass error (the difference between the average of measured m/z and the theoretical ion mass) of ± 1.5 ppm. When multiple formulas were assigned to a peak within the mass error range, the one with the highest confidence score was selected.&lt;/p&gt; &lt;p&gt;&lt;strong&gt;Feature list generation:&lt;/strong&gt;&lt;br /&gt; With assignments for all samples, a combined list of features (molecular formula assigned m/z peak at a specific retention time interval) was constructed to compare molecular abundance across samples. Features that represent the same mass peak but were assigned with different formulas in different samples were consolidated by choosing the assignment with the highest confidence score. Elemental ratios (O/C, N/C, and H/C) of each feature were calculated based on the molecular formula. Features were classified into different molecular classes based on their stoichiometric ratios. After removing isotopologues, features that satisfied the following criteria were noted as clean features and exported as a clean feature list for downstream analysis: (1) is detected in more than 4 samples, (2) is within the mass range of 100-580 m/z, (3) has a max intensity greater than 20,000, (4) had m/z error within 4 times the standard error of the expected error (expected error is a rolling average of features across the dataset), (5) max intensity in the sample is 5 times greater than in blank samples.&lt;/p&gt; Specified by the Principal Investigator(s) - Imported original file &quot;Clean_Featurelist.csv&quot; into the BCO-DMO data processing system. - Renamed fields to comply with BCO-DMO naming conventions. - Saved the final file as &quot;1004302_v1_sting_sgd_river_lcms_dom.csv&quot;. Specified by BCO-DMO Data Managers asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact centrifuge centrifuge PI Supplied Instrument Name: centrifuge Instrument Name: Centrifuge Instrument Short Name: Instrument Description: A machine with a rapidly rotating container that applies centrifugal force to its contents, typically to separate fluids of different densities (e.g., cream from milk) or liquids from solids. Thermo Scientific Orbitrap IQ-X Mass Spectrometer Thermo Scientific Orbitrap IQ-X Mass Spectrometer PI Supplied Instrument Name: Thermo Scientific Orbitrap IQ-X Mass Spectrometer PI Supplied Instrument Description:Dissolved organic matter composition was analyzed using a Thermo Scientific Orbitrap IQ-X Mass Spectrometer coupled to a Vanquish Horizon liquid chromatography system. Instrument Name: Mass Spectrometer Instrument Short Name:Mass Spec Instrument Description: General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/ submersible pump submersible pump PI Supplied Instrument Name: submersible pump PI Supplied Instrument Description:Surface water samples (500mL) were collected at all river stations via a submersible pump lowered ~1.0 m below the water’s surface. Instrument Name: Pump Instrument Short Name: Instrument Description: A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps peristaltic pump peristaltic pump PI Supplied Instrument Name: peristaltic pump PI Supplied Instrument Description:Surface water, bottom water, and submarine groundwater samples (2L) were collected via peristaltic pump along three coastal transects. Instrument Name: Pump Instrument Short Name: Instrument Description: A pump is a device that moves fluids (liquids or gases), or sometimes slurries, by mechanical action. Pumps can be classified into three major groups according to the method they use to move the fluid: direct lift, displacement, and gravity pumps Vanquish Horizon liquid chromatography system Vanquish Horizon liquid chromatography system PI Supplied Instrument Name: Vanquish Horizon liquid chromatography system PI Supplied Instrument Description:Dissolved organic matter composition was analyzed using a Thermo Scientific Orbitrap IQ-X Mass Spectrometer coupled to a Vanquish Horizon liquid chromatography system. Instrument Name: Ultra-high-performance liquid chromatography Instrument Short Name:UHPLC Instrument Description: Ultra high-performance liquid chromatography: Column chromatography where the mobile phase is a liquid, the stationary phase consists of very small (< 2 microm) particles and the inlet pressure is relatively high.