http://lod.bco-dmo.org/id/dataset/1005186 eng; USA utf8 dataset Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact 2026-08-13 ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data ISO 19115-2:2009(E) Single-cell elemental quotas and stoichiometry of Synechococcus sp. WH8102 measured by X-ray microanalysis (XRMA) under varying phosphorus conditions 2026-08-14 publication 2026-08-14 revision BCO-DMO Linked Data URI 2026-08-14 creation http://lod.bco-dmo.org/id/dataset/1005186 Solange Duhamel University of Arizona principalInvestigator Julia Diaz University of California-San Diego Scripps principalInvestigator Alba Filella University of Arizona principalInvestigator Mariona Segura-Noguera Mediterranean Institute for Advanced Studies principalInvestigator Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. publisher Cite this dataset as: Filella, A., Duhamel, S., Segura-Noguera, M., Diaz, J. (2026) Single-cell elemental quotas and stoichiometry of Synechococcus sp. WH8102 measured by X-ray microanalysis (XRMA) under varying phosphorus conditions. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-08-14 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/1005186 [access date] Single-cell Synechococcus WH8102 XRMA elemental data Dataset Description: Methods and Sampling: &lt;p&gt;For XRMA analysis, ~ 0.4 mL of &lt;em&gt;Synechococcus&lt;/em&gt; culture cells were transferred to a 1.5 mL microtube and washed with 0.8 mL of cold buffered ultrapure water (pH 8) to remove salts. After centrifugation (1 min at 7000 rpm at 15 ºC), 1.1 mL supernatant was replaced by cold buffered ultrapure water and centrifuged under the same conditions. This last step was repeated twice, and 1.1 mL was carefully discarded. The concentrated cells were homogenized before transferring 6 µL onto a 3 mm TEM grid coated with formvar and evaporated carbon (Ted Pella, Redding, CA, USA). The grid was air-dried and stored in a vacuum cabinet until analysis. Cells were analyzed for elemental composition on a Hitachi SU8600 Field-Emission Scanning Electron Microscope (FE-SEM) equipped with two high-resolution (123 eV) Bruker X-Flash 7|30 Energy Dispersive Spectrometers. To avoid interference with the holder, the grids were attached to the edge of the SEM pins. XRMA spectra were obtained at 20 kV of incident energy during 90 seconds of live time. The absolute beam current was measured using a Faraday cup before each analysis session, which allowed for correcting for beam current drift during the session, and ensured an accurate reference to the calibration. Polystyrene latex spheres with mean particle size of 0.6 and 1.1 µm&amp;nbsp; (Sigma LB6 and LB11) were used to calibrate carbon, and ATP (Sigma, A2383) was used to obtain the calibration constants for N and P, following Norland et al. (1995).&amp;nbsp;&lt;/p&gt; &lt;p&gt;Cell dimensions were measured from SEM micrographs using ImageJ (version 1.54g, Abràmoff et al. 2004). &lt;em&gt;Synechococcus &lt;/em&gt;cells were assumed to exhibit a rod-shaped morphology and were therefore approximated geometrically as a cylinder with two hemispherical ends. Based on these measurements, cellular biovolumes were calculated using standard geometric approximations for this morphology.&lt;/p&gt; Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2245249 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2245249 onGoing Solange Duhamel University of Arizona 520-621-6047 1007 E Lowell Street Life Sciences South 315 Tucson AZ 85721 USA duhamel@arizona.edu pointOfContact Julia Diaz University of California-San Diego Scripps j2diaz@ucsd.edu pointOfContact Alba Filella University of Arizona 689585260 afilella@arizona.edu pointOfContact Mariona Segura-Noguera Mediterranean Institute for Advanced Studies (808) 956-0566 Center for Microbial Oceanography: Research and Education Department of Oceanography SOEST University of Hawai'i at Manoa C·MORE Hale, 1950 East-West Road Honolulu HI 96822 USA mariona@imedea.uib-csic.es pointOfContact asNeeded Dataset Version: 1 Unknown SampleID Syn_Cell exp time rep A_um2_xray A_um2_imageJ Volume_um3 C_fg_cell N_fg_cell P_fg_cell C_fg_um3 N_fg_um3 P_fg_um3 C_P C_N N_P centrifuge homogenizer Hitachi SU8600 Field-Emission Scanning Electron Microscope (FE-SEM) theme None, User defined sample identification treatment time_point replicate area volume Elemental carbon (C) Nitrogen Phosphorus Carbon to Phosphorus ratio (C:P) Carbon to Nitrogen ratio (C:N) Nitrogen to phosphorus ratio (N:P) featureType BCO-DMO Standard Parameters Centrifuge Homogenizer Scanning Electron Microscope instrument BCO-DMO Standard Instruments otherRestrictions otherRestrictions Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use. Collaborative Research: Assessing the role of polyphosphate production and cycling in marine ecosystem functioning https://osprey.bco-dmo.org/project/891607 Collaborative Research: Assessing the role of polyphosphate production and cycling in marine ecosystem functioning <p><em>NSF Award Abstract:</em><br /> Phosphorus (P) is a vital nutrient required by all forms of life. In the ocean, which sustains half of global photosynthesis and oxygen supply, P can be scarce enough to constrain biological productivity, carbon dioxide uptake, and therefore climate. Human activity is accelerating the delivery of nutrients like P to the ocean, but rates of nitrogen inputs are even greater. This imbalance may increase levels of P stress in marine ecosystems, placing ocean productivity more and more under the control of P supply. Given the critical role of P in sustaining ocean health and ecosystem services now and into the future, a comprehensive understanding of its utilization and fate in the marine environment is necessary. In this project, the research team investigates marine polyphosphate (polyP), a ubiquitous yet poorly understood form of P made by all living organisms. To close major knowledge gaps on marine polyP, the investigators are overcoming major technical barriers to produce the first quantitative measurements of polyP in marine microorganisms. These measurements are being conducted on laboratory microbial cultures, as well as field samples from environments with high P supply, such as the California Current Ecosystem, or very low P supply, such as the Mediterranean Sea. In addition, the research team is resolving the cellular function of marine polyP across these different organisms and environments in order to clarify its role in biological P nutrition. This work helps advance polyP research across disciplines, including terrestrial science and even cancer research, and has broad application to P bioremediation. This project supports a postdoctoral researcher, a graduate student, and several undergraduate students in the labs of two female scientists. New educational tools to teach the public about marine polyP are being produced and disseminated through this project. A K12 teacher is participating in the work and communicating the findings to their classrooms and to broad audiences online.</p> <p>PolyP is ubiquitous in marine systems, where it plays critical roles in microbial P nutrition and P mineral formation and sequestration. In these ways, polyP has the potential to shape long- and short-term marine P cycling, primary productivity, microbial ecology, and global climate. However, major knowledge gaps still exist. Due to technical limitations, the scientific community currently lacks a quantitative understanding of marine polyP pools, their chain lengths, and biological origins. Furthermore, given the view of polyP as a P storage molecule, recent observations pointing to the preferential retention of particulate polyP in low phosphate (Pi) environments raise new questions about its ecophysiological functions. To close these knowledge gaps, two research questions are addressed: Q1: What is the total content and chain length distribution of polyP across different microbial groups and environmental conditions? Q2: How do the production of particulate polyP contribute to microbial P demand and stoichiometry across a broad range in Pi availability? The following hypotheses are tested: H1 (Q1): Functionally and environmentally diverse plankton produce a broad range of polyP chain lengths and concentrations. H2 (Q2): The preferential retention of polyP in low Pi environments can be reconciled with its role as a P storage molecule by a combination of taxonomic and physiological factors. These hypotheses are tested in the laboratory using representative cultures of marine plankton and in the field using observational approaches along natural Pi gradients in the Pacific Ocean and Mediterranean Sea. Applying a new P-targeted method using mass spectrometry, the team is resolving an unprecedented level of detail in marine polyP content and speciation. By combining cell sorting with elemental, biochemical, and radiotracer analyses, the team is gaining a mechanistic understanding of polyP physiology and its cycling in the ocean.</p> <p>This project is supported by the Biological Oceanography and Chemical Oceanography Programs. This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p> Polyphosphate production and cycling largerWorkCitation project eng; USA biota oceans 2026-08-14 Mediterranean Sea and California Current Ecosystem 0 BCO-DMO catalogue of parameters from Single-cell elemental quotas and stoichiometry of Synechococcus sp. WH8102 measured by X-ray microanalysis (XRMA) under varying phosphorus conditions Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact http://lod.bco-dmo.org/id/dataset-parameter/1005428.rdf Name: SampleID Units: unitless Description: <p>Unique identifier for each analyzed single-cell sample</p> http://lod.bco-dmo.org/id/dataset-parameter/1005429.rdf Name: Syn_Cell Units: unitless Description: <p>Cell index within sample or field of view</p> http://lod.bco-dmo.org/id/dataset-parameter/1005430.rdf Name: exp Units: unitless Description: <p>Experimental treatment condition (e.g., Low P, Mid P, High P)</p> http://lod.bco-dmo.org/id/dataset-parameter/1005431.rdf Name: time Units: days Description: <p>Sampling timepoint in days</p> http://lod.bco-dmo.org/id/dataset-parameter/1005432.rdf Name: rep Units: unitless Description: <p>Biological replicate identifier</p> http://lod.bco-dmo.org/id/dataset-parameter/1005433.rdf Name: A_um2_xray Units: µm2 Description: <p>Cell projected area measured directly from SEM/XRMA system</p> http://lod.bco-dmo.org/id/dataset-parameter/1005434.rdf Name: A_um2_imageJ Units: µm2 Description: <p>Cell projected area recalculated using ImageJ image analysis</p> http://lod.bco-dmo.org/id/dataset-parameter/1005435.rdf Name: Volume_um3 Units: µm3 Description: <p>Estimated cell volume calculated assuming rod-shaped geometry</p> http://lod.bco-dmo.org/id/dataset-parameter/1005436.rdf Name: C_fg_cell Units: femtograms (fg C / cell) Description: <p>Carbon content per cell</p> http://lod.bco-dmo.org/id/dataset-parameter/1005437.rdf Name: N_fg_cell Units: femtograms (fg N / cell) Description: <p>Nitrogen content per cell</p> http://lod.bco-dmo.org/id/dataset-parameter/1005438.rdf Name: P_fg_cell Units: femtograms (fg P / cell) Description: <p>Phosphorus content per cell</p> http://lod.bco-dmo.org/id/dataset-parameter/1005439.rdf Name: C_fg_um3 Units: fg C / µm3 Description: <p>Carbon concentration normalized to cell volume</p> http://lod.bco-dmo.org/id/dataset-parameter/1005440.rdf Name: N_fg_um3 Units: fg N / µm3 Description: <p>Nitrogen concentration normalized to cell volume</p> http://lod.bco-dmo.org/id/dataset-parameter/1005441.rdf Name: P_fg_um3 Units: fg P / µm3 Description: <p>Phosphorus concentration normalized to cell volume</p> http://lod.bco-dmo.org/id/dataset-parameter/1005442.rdf Name: C_P Units: ratio (unitless) Description: <p>Carbon-to-phosphorus molar or mass ratio</p> http://lod.bco-dmo.org/id/dataset-parameter/1005443.rdf Name: C_N Units: ratio (unitless) Description: <p>Carbon-to-nitrogen molar or mass ratio</p> http://lod.bco-dmo.org/id/dataset-parameter/1005444.rdf Name: N_P Units: ratio (unitless) Description: <p>Nitrogen-to-phosphorus molar or mass ratio</p> GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact https://osprey.bco-dmo.org/dataset/1005186/data/download download onLine dataset &lt;p&gt;For XRMA analysis, ~ 0.4 mL of &lt;em&gt;Synechococcus&lt;/em&gt; culture cells were transferred to a 1.5 mL microtube and washed with 0.8 mL of cold buffered ultrapure water (pH 8) to remove salts. After centrifugation (1 min at 7000 rpm at 15 ºC), 1.1 mL supernatant was replaced by cold buffered ultrapure water and centrifuged under the same conditions. This last step was repeated twice, and 1.1 mL was carefully discarded. The concentrated cells were homogenized before transferring 6 µL onto a 3 mm TEM grid coated with formvar and evaporated carbon (Ted Pella, Redding, CA, USA). The grid was air-dried and stored in a vacuum cabinet until analysis. Cells were analyzed for elemental composition on a Hitachi SU8600 Field-Emission Scanning Electron Microscope (FE-SEM) equipped with two high-resolution (123 eV) Bruker X-Flash 7|30 Energy Dispersive Spectrometers. To avoid interference with the holder, the grids were attached to the edge of the SEM pins. XRMA spectra were obtained at 20 kV of incident energy during 90 seconds of live time. The absolute beam current was measured using a Faraday cup before each analysis session, which allowed for correcting for beam current drift during the session, and ensured an accurate reference to the calibration. Polystyrene latex spheres with mean particle size of 0.6 and 1.1 µm&amp;nbsp; (Sigma LB6 and LB11) were used to calibrate carbon, and ATP (Sigma, A2383) was used to obtain the calibration constants for N and P, following Norland et al. (1995).&amp;nbsp;&lt;/p&gt; &lt;p&gt;Cell dimensions were measured from SEM micrographs using ImageJ (version 1.54g, Abràmoff et al. 2004). &lt;em&gt;Synechococcus &lt;/em&gt;cells were assumed to exhibit a rod-shaped morphology and were therefore approximated geometrically as a cylinder with two hemispherical ends. Based on these measurements, cellular biovolumes were calculated using standard geometric approximations for this morphology.&lt;/p&gt; Specified by the Principal Investigator(s) &lt;p&gt;Latex spheres with a 0.6 µm mean particle size were used as internal standards and were analyzed at the start and end of each sample analysis session. Detection limits were calculated as 3 times the standard deviation of the blank (formvar film) measured in the energy region of each target element's spectral peak and were 26.3, 0.54, and 0.45 fg cell&lt;sup&gt;-1&lt;/sup&gt; for C, N, and P, respectively. In the present study, only elemental quotas above the detection limit, which account for more than 98% of the quotas measured, are shown and used to calculate ratios.&amp;nbsp;&lt;/p&gt; Specified by the Principal Investigator(s) - Loaded Sheet1 from xray_syn_wh8102_dataset_bco.xlsx into table named xrma_single_cell_stoichiometry; configured empty string and &quot;nd&quot; as missing values; applied floating point error adjustment and preserved formatting during load - Renamed column &quot;Exp&quot; to &quot;exp&quot; to match the lowercase column name used in the README, since the actual data file used &quot;Exp&quot; as the treatment column header - Output as 1005186_v1_xrma_single_cell_stoichiometry.csv Specified by BCO-DMO Data Managers asNeeded 7.x-1.1 Biological and Chemical Oceanography Data Management Office (BCO-DMO) Unavailable 508-289-2009 WHOI MS#36 Woods Hole MA 02543 USA info@bco-dmo.org http://www.bco-dmo.org Monday - Friday 8:00am - 5:00pm For questions regarding this resource, please contact BCO-DMO via the email address provided. pointOfContact centrifuge centrifuge PI Supplied Instrument Name: centrifuge PI Supplied Instrument Description:After centrifugation (1 min at 7000 rpm at 15 ºC), 1.1 mL supernatant was replaced by cold buffered ultrapure water and centrifuged under the same conditions. Instrument Name: Centrifuge Instrument Short Name: Instrument Description: A machine with a rapidly rotating container that applies centrifugal force to its contents, typically to separate fluids of different densities (e.g., cream from milk) or liquids from solids. homogenizer homogenizer PI Supplied Instrument Name: homogenizer PI Supplied Instrument Description:The concentrated cells were homogenized before transferring 6 µL onto a 3 mm TEM grid coated with formvar and evaporated carbon (Ted Pella, Redding, CA, USA). Instrument Name: Homogenizer Instrument Short Name:Homogenizer Instrument Description: A homogenizer is a piece of laboratory equipment used for the homogenization of various types of material, such as tissue, plant, food, soil, and many others. Hitachi SU8600 Field-Emission Scanning Electron Microscope (FE-SEM) Hitachi SU8600 Field-Emission Scanning Electron Microscope (FE-SEM) PI Supplied Instrument Name: Hitachi SU8600 Field-Emission Scanning Electron Microscope (FE-SEM) PI Supplied Instrument Description:Cells were analyzed for elemental composition on a Hitachi SU8600 Field-Emission Scanning Electron Microscope (FE-SEM) equipped with two high-resolution (123 eV) Bruker X-Flash 7|30 Energy Dispersive Spectrometers. Instrument Name: Scanning Electron Microscope Instrument Short Name:SEM Instrument Description: A scanning electron microscope (SEM) scans a focused electron beam over a surface to create an image. The electrons in the beam interact with the sample, producing various signals that can be used to obtain information about the surface topography and composition.