http://lod.bco-dmo.org/id/dataset/1005988
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2026-08-25
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Chemoautotrophic assimilation of inorganic carbon within the ETNP OMZ R/V Atlantis AT50-08 from the Eastern Tropical Pacific Minimum Oxygen Zone from February to March 2023 (MicroPro project)
2026-08-25
publication
2026-08-25
revision
BCO-DMO Linked Data URI
2026-08-25
creation
http://lod.bco-dmo.org/id/dataset/1005988
Maria G. Pachiadaki
Woods Hole Oceanographic Institution
principalInvestigator
Gordon T. Taylor
Stony Brook University
principalInvestigator
Elena Yakubovskaya
Stony Brook University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Taylor, G. T., Pachiadaki, M. G., Yakubovskaya, E. (2026) Chemoautotrophic assimilation of inorganic carbon within the ETNP OMZ R/V Atlantis AT50-08 from the Eastern Tropical Pacific Minimum Oxygen Zone from February to March 2023 (MicroPro project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-08-25 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/1005988 [access date]
Chemoautotrophic assimilation of inorganic carbon within the ETNP OMZ R/V Atlantis AT50-08 Dataset Description: Methods and Sampling: <p>1L whole water samples were collected on deck under nitrogen pressure at the rosette. A three-volume overflow was performed for each sample to minimize oxygen contamination. Bottles were sealed with deoxygenated butyl rubber septa after confirming all bubbles were excluded. A He gas headspace was added in the shipboard lab no more than 15 minutes after collection. To quantify community chemoautotrophic activity, triplicate incubations with <sup>14</sup>C-bicarbonate were prepared in 16 mL Hungate tubes with degassed butyl rubber septa under nitrogen pressure. Chilled N2-purged <sup>14</sup>C-bicarbonate in an alkaline brine (pH 9.5; S = 60 on the practical salinity scale) was injected (0.92 µCi Mex1, 0.54 µCi Mex2) into the bottom before sealing (Tuttle and Jannasch 1973a). One depth per cast was selected for stimulation experiments with alternative electron donors. Selected incubations were spiked with <sup>14</sup>C-bicarbonate and one of the following: NH<sub>4</sub><sup>+</sup>, DMSP, H<sub>2</sub>S or S<sub>2</sub>O<sub>3</sub>. Incubations were conducted in a dark ice chest for 18-24 hours and terminated by filtration onto 25 mm nitrocellulose 0.22 µm filters (25 mm diameter Osmonics). Selected non-amended incubations were size fractionated into a “particle-associated” fraction (&gt;2.7 µm) and a free-living size fraction (0.22 – 2.7 µm) by sequential filtration through stacked 25 mm Swinnex filter holders. Unassimilated <sup>14</sup>C-bicarbonate was purged from the filters in a saturated HCl atmosphere enclosure for &gt;1h in a fume hood. Filters were then dried, suspended in scintillation cocktail (Hionic-Fluor) and radioassayed. Table 1 summarizes sample types. Data were corrected for isotopic fractionation (multiplied by 1.06) and for nonbiological sorption by use of control samples processed immediately after introduction of the radiotracer (T<sub>0</sub> samples). If blanks were higher than T<sub>0</sub> samples, blanks were used instead. Three T<sub>0</sub> sample sets were made, one for standard+stimulation experiments, one for the free-living size fraction and one for the particle-associated size fraction. Rates of dark <sup>14</sup>C-assimilation were normalized to total nanomoles of carbon per day via equation detailed under 'Data Processing' section.</p>
<table>
<tbody>
<tr>
<td><strong>Sample code</strong></td>
<td><strong>Tracer/Amendments</strong></td>
<td><strong>Experiment type</strong></td>
<td><strong>Notes</strong></td>
</tr>
<tr>
<td>standard</td>
<td><sup>14</sup>C-bicarbonate</td>
<td>Standard</td>
<td>&nbsp;</td>
</tr>
<tr>
<td>S<sub>2</sub>O<sub>3</sub></td>
<td>
<p><sup>14</sup>C-bicarbonate and</p>
<p>S<sub>2</sub>O<sub>3</sub></p>
</td>
<td>Alternative electron donor amendment</td>
<td>Target stimulant concentration: 10uM. For all samples prepared Feb.19 or later, target concentration is 5uM</td>
</tr>
<tr>
<td>DMSP</td>
<td><sup>14</sup>C-bicarbonate and DMSP</td>
<td>Alternative electron donor amendment</td>
<td>Target stimulant concentration: 100nM. For all samples prepared Feb.19 or later, target concentration is 50nM</td>
</tr>
<tr>
<td>NH<sub>4</sub><sup>+</sup></td>
<td><sup>14</sup>C-bicarbonate and NH<sub>4</sub><sup>+</sup></td>
<td>Alternative electron donor amendment</td>
<td>Target stimulant concentration: 10uM. For all samples prepared Feb.19 or later, target concentration is 5uM</td>
</tr>
<tr>
<td>H<sub>2</sub>S</td>
<td><sup>14</sup>C-bicarbonate and H<sub>2</sub>S</td>
<td>Alternative electron donor amendment</td>
<td>Target stimulant concentration: 10uM. For all samples prepared Feb.19 or later, target concentration is 5uM</td>
</tr>
<tr>
<td>0.2_SF</td>
<td><sup>14</sup>C-bicarbonate</td>
<td>Size fractionation</td>
<td>&nbsp;</td>
</tr>
<tr>
<td>2.7_SF</td>
<td><sup>14</sup>C-bicarbonate</td>
<td>Size fractionation</td>
<td>&nbsp;</td>
</tr>
</tbody>
</table>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1924492 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1924492
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1924424 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1924424
onGoing
Maria G. Pachiadaki
Woods Hole Oceanographic Institution
508-289-2943
Redfield Lab
Woods Hole
MA
02543
USA
mpachiadaki@whoi.edu
pointOfContact
Gordon T. Taylor
Stony Brook University
631-632-8688
Stony Brook University School of Marine and Atmospheric Sciences
Stony Brook
NY
11794-5000
USA
gordon.taylor@stonybrook.edu
pointOfContact
Elena Yakubovskaya
Stony Brook University
elena.yakubovskaya@stonybrook.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Depth
Sample_Type
Station
Lat
Long
ISO_Cast_Start_DateTime_UTC
Cast_Date_UTC
Cast_Time_UTC
CTD_Cast_Filename
Incubation_Start_Local_Ship_Time
Incubation_Stop_Local_Ship_Time
Incubation_Duration
Raw_DPM
Assimilation_nMC
Assimilation_sd_nMC
Replicate_ID
Scintillation counter: TRICARBB281000, serial number SGTC05150535
theme
None, User defined
depth
sample type
station
latitude
longitude
ISO_DateTime_UTC
start_date
time_start
file_name
time_start_local
time_end_local
duration
No BCO-DMO term
replicate
featureType
BCO-DMO Standard Parameters
Liquid Scintillation Counter
instrument
BCO-DMO Standard Instruments
AT50-08B
service
Deployment Activity
Eastern Tropical Pacific, Minimum Oxygen Zone (OMZ)
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Key Microbial Processes in Oxygen Minimum Zones: From In Situ Community Rate Measurements to Single Cells
https://osprey.bco-dmo.org/project/828777
Collaborative Research: Key Microbial Processes in Oxygen Minimum Zones: From In Situ Community Rate Measurements to Single Cells
<p>NSF Award Abstract:</p>
<p>Oxygen availability shapes the distributions and activities of marine organisms. Ongoing human activities and climate change are expected to lead to expansion and intensification of already large oxygen-stressed areas of the coastal and open ocean. Decreases in ocean oxygen have significant ecological consequences, including habitat loss for migratory and bottom-dwelling organisms, modification of the marine food web, and production of trace gases with pronounced feedbacks on climate, such as methane and nitrous oxide. Intense chemical cycling by microorganisms occurs in oxygen-depleted marine habitats. However, a full understanding of the consequences for marine ecosystems is hampered by limited knowledge of actual rates of key microbiological processes and dynamics of the microorganisms mediating them. This study combines novel methods and sampling techniques to understand how these processes are influenced by changes in oxygen concentration to inform predictions of important chemical exchanges within a changing ocean and its production of climate-active gases. This deeply collaborative project trains undergraduates (four of whom participate on the cruise), a graduate student and a postdoctoral fellow. Outreach takes place in middle and high schools and through social media. Data and samples from the cruise are integrated in coursework.</p>
<p>Oxygen depletion alters cycling of major elements (especially carbon, nitrogen, and sulfur) as well as food web functionality. This project addresses major gaps in our knowledge of oxygen minimum zone (OMZ) processes by applying in situ approaches to more accurately measure rates of several key microbial processes (chemoautotrophy, denitrification, anammox, sulfate reduction and sulfide oxidation) central to marine biogeochemical cycling. This work studies the Eastern Tropical North Pacific OMZ, the largest open ocean oxygen-depleted system, to 1) determine the in situ rates of microbial processes involved in carbon, nitrogen, and sulfur cycling, 2) reveal the genomic blueprint of active single cells involved in these processes, and 3) obtain estimates of the relative contributions of the dominant chemoautotrophic and heterotrophic groups to the measured rates. This work include applies cutting-edge equipment for in situ sampling and incubations that minimize artifacts associated with traditional water sampling approaches, allowing more accurate estimates of rates of important biogeochemical processes. Additionally, rate measurements of relatively undisturbed bulk and fractionated water samples make it easier to distinguish the potential role of particle-associated microorganisms in these OMZ processes. Single cell sorting of microorganisms using a fluorescent dye indicative of cell activity together with metatranscriptomics informs on metabolic pathways used for key processes by active microbial community members, as well as the potential coupling of chemoautotrophy and nitrogen or/and sulfur cycling. By combining stable isotope probing, fluorescence in situ hybridization and single cell Raman microspectrometry the relative activity levels of different microbial phylotypes involved in chemoautotrophic and heterotrophic elemental cycling are assessed.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
MicroPro
largerWorkCitation
project
eng; USA
oceans
Eastern Tropical Pacific, Minimum Oxygen Zone (OMZ)
-106.287
-104.89533
18.17683
18.887
2023-02-16
2023-03-10
East Tropical North Pacific Ocean
0
BCO-DMO catalogue of parameters from Chemoautotrophic assimilation of inorganic carbon within the ETNP OMZ R/V Atlantis AT50-08 from the Eastern Tropical Pacific Minimum Oxygen Zone from February to March 2023 (MicroPro project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/1006143.rdf
Name: Depth
Units: meters (m)
Description: <p>Recorded depth at which Niskin bottle was closed and water sample was taken. Manually recorded from CTD cast at time of sample acquisition.</p>
http://lod.bco-dmo.org/id/dataset-parameter/1006145.rdf
Name: Sample_Type
Units: unitless
Description: <p>Denotes type of sample, full description available in Table 1 of methods section</p>
http://lod.bco-dmo.org/id/dataset-parameter/1006146.rdf
Name: Station
Units: unitless
Description: <p>Broadly categorizes cast location into 'Mex1' or 'Mex2', which corresponds to a nearshore or an offshore station respectively.</p>
http://lod.bco-dmo.org/id/dataset-parameter/1006147.rdf
Name: Lat
Units: decimal degrees
Description: <p>Latitude at which sample was taken, recorded in CTD cast header. A positive value indicates a northern latitude coordinate.</p>
http://lod.bco-dmo.org/id/dataset-parameter/1006149.rdf
Name: Long
Units: decimal degrees
Description: <p>Longitude at which sample was taken, recorded in CTD cast header. A negative value indicates a western longitude coordinate.</p>
http://lod.bco-dmo.org/id/dataset-parameter/1006151.rdf
Name: ISO_Cast_Start_DateTime_UTC
Units: unitless
Description: <p>Start datetime of the associated cast in UTC. The values of this column were created by combining the original Cast Date and Cast Time column.</p>
http://lod.bco-dmo.org/id/dataset-parameter/1006153.rdf
Name: Cast_Date_UTC
Units: unitless
Description: <p>Date the cast was made as recorded by CTD cast.</p>
http://lod.bco-dmo.org/id/dataset-parameter/1006154.rdf
Name: Cast_Time_UTC
Units: unitless
Description: <p>Time the cast began recorded by CTD cast.</p>
http://lod.bco-dmo.org/id/dataset-parameter/1006155.rdf
Name: CTD_Cast_Filename
Units: unitless
Description: <p>Filename associated with CTD cast corresponding to these samples.</p>
http://lod.bco-dmo.org/id/dataset-parameter/1006156.rdf
Name: Incubation_Start_Local_Ship_Time
Units: unitless
Description: <p>The time (local time of the ship at the time of recording) the incubation was started by injecting tracer in local time, recorded to calculate incubation duration.</p>
http://lod.bco-dmo.org/id/dataset-parameter/1006157.rdf
Name: Incubation_Stop_Local_Ship_Time
Units: unitless
Description: <p>The time (local time of the ship at the time of recording) the incubation was terminated by filtration in local time, recorded to calculate incubation duration.</p>
http://lod.bco-dmo.org/id/dataset-parameter/1006158.rdf
Name: Incubation_Duration
Units: hours
Description: <p>Incubation duration - the length of time the sample was incubated with the tracer and (if applicable) alternative electron donor</p>
http://lod.bco-dmo.org/id/dataset-parameter/1006159.rdf
Name: Raw_DPM
Units: disintegrations per minute
Description: <p>Disintegrations per minute for each sample, recorded from scintillation counter output.</p>
http://lod.bco-dmo.org/id/dataset-parameter/1006160.rdf
Name: Assimilation_nMC
Units: nanomole carbon per day
Description: <p>Calculated mean carbon assimilation.</p>
http://lod.bco-dmo.org/id/dataset-parameter/1006161.rdf
Name: Assimilation_sd_nMC
Units: nanomole carbon per day
Description: <p>Standard deviation was calculated by taking the sum of all assimilation values (nM C d⁻¹), dividing it by the number of samples, squaring the result, and then taking the square root (Standard deviation = sqrt(((sum of all assimilation_nMC_d^-1)^2)/#samples).</p>
http://lod.bco-dmo.org/id/dataset-parameter/1006162.rdf
Name: Replicate_ID
Units: unitless
Description: <p>Triplicate identifier.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://osprey.bco-dmo.org/dataset/1005988/data/download
download
onLine
dataset
<p>1L whole water samples were collected on deck under nitrogen pressure at the rosette. A three-volume overflow was performed for each sample to minimize oxygen contamination. Bottles were sealed with deoxygenated butyl rubber septa after confirming all bubbles were excluded. A He gas headspace was added in the shipboard lab no more than 15 minutes after collection. To quantify community chemoautotrophic activity, triplicate incubations with <sup>14</sup>C-bicarbonate were prepared in 16 mL Hungate tubes with degassed butyl rubber septa under nitrogen pressure. Chilled N2-purged <sup>14</sup>C-bicarbonate in an alkaline brine (pH 9.5; S = 60 on the practical salinity scale) was injected (0.92 µCi Mex1, 0.54 µCi Mex2) into the bottom before sealing (Tuttle and Jannasch 1973a). One depth per cast was selected for stimulation experiments with alternative electron donors. Selected incubations were spiked with <sup>14</sup>C-bicarbonate and one of the following: NH<sub>4</sub><sup>+</sup>, DMSP, H<sub>2</sub>S or S<sub>2</sub>O<sub>3</sub>. Incubations were conducted in a dark ice chest for 18-24 hours and terminated by filtration onto 25 mm nitrocellulose 0.22 µm filters (25 mm diameter Osmonics). Selected non-amended incubations were size fractionated into a “particle-associated” fraction (&gt;2.7 µm) and a free-living size fraction (0.22 – 2.7 µm) by sequential filtration through stacked 25 mm Swinnex filter holders. Unassimilated <sup>14</sup>C-bicarbonate was purged from the filters in a saturated HCl atmosphere enclosure for &gt;1h in a fume hood. Filters were then dried, suspended in scintillation cocktail (Hionic-Fluor) and radioassayed. Table 1 summarizes sample types. Data were corrected for isotopic fractionation (multiplied by 1.06) and for nonbiological sorption by use of control samples processed immediately after introduction of the radiotracer (T<sub>0</sub> samples). If blanks were higher than T<sub>0</sub> samples, blanks were used instead. Three T<sub>0</sub> sample sets were made, one for standard+stimulation experiments, one for the free-living size fraction and one for the particle-associated size fraction. Rates of dark <sup>14</sup>C-assimilation were normalized to total nanomoles of carbon per day via equation detailed under 'Data Processing' section.</p>
<table>
<tbody>
<tr>
<td><strong>Sample code</strong></td>
<td><strong>Tracer/Amendments</strong></td>
<td><strong>Experiment type</strong></td>
<td><strong>Notes</strong></td>
</tr>
<tr>
<td>standard</td>
<td><sup>14</sup>C-bicarbonate</td>
<td>Standard</td>
<td>&nbsp;</td>
</tr>
<tr>
<td>S<sub>2</sub>O<sub>3</sub></td>
<td>
<p><sup>14</sup>C-bicarbonate and</p>
<p>S<sub>2</sub>O<sub>3</sub></p>
</td>
<td>Alternative electron donor amendment</td>
<td>Target stimulant concentration: 10uM. For all samples prepared Feb.19 or later, target concentration is 5uM</td>
</tr>
<tr>
<td>DMSP</td>
<td><sup>14</sup>C-bicarbonate and DMSP</td>
<td>Alternative electron donor amendment</td>
<td>Target stimulant concentration: 100nM. For all samples prepared Feb.19 or later, target concentration is 50nM</td>
</tr>
<tr>
<td>NH<sub>4</sub><sup>+</sup></td>
<td><sup>14</sup>C-bicarbonate and NH<sub>4</sub><sup>+</sup></td>
<td>Alternative electron donor amendment</td>
<td>Target stimulant concentration: 10uM. For all samples prepared Feb.19 or later, target concentration is 5uM</td>
</tr>
<tr>
<td>H<sub>2</sub>S</td>
<td><sup>14</sup>C-bicarbonate and H<sub>2</sub>S</td>
<td>Alternative electron donor amendment</td>
<td>Target stimulant concentration: 10uM. For all samples prepared Feb.19 or later, target concentration is 5uM</td>
</tr>
<tr>
<td>0.2_SF</td>
<td><sup>14</sup>C-bicarbonate</td>
<td>Size fractionation</td>
<td>&nbsp;</td>
</tr>
<tr>
<td>2.7_SF</td>
<td><sup>14</sup>C-bicarbonate</td>
<td>Size fractionation</td>
<td>&nbsp;</td>
</tr>
</tbody>
</table>
Specified by the Principal Investigator(s)
<p><strong>Assimilation equation:&nbsp;</strong></p>
<p>rate (nanomole carbon per liter per day) = (((2*10^6) * 1.06 * max(DPM - blank or t0, 0))/specific activity value)*(24/incubation time)</p>
<p>&nbsp;</p>
<p><strong>Constants:&nbsp;</strong></p>
<p>2*10^6 = dissolved inorganic carbon in seawater (from Feely et al. 1995)</p>
<p>1.06 = isotopic fractionation factor</p>
<p>29 = standard blank in DPM</p>
<p>30 = small size fraction blank in DPM</p>
<p>37 = large size fraction blank in DPM</p>
<p>2032708 = specific activity for mex1 in DPM, 0.916 microCurie injected per sample</p>
<p>1190572 = specific activity for mex2 in DPM, 0.536 microCurie injected per sample</p>
<p>&nbsp;</p>
<p>&nbsp;</p>
<p>Notes:&nbsp;</p>
<p>-separate specific activities were used for Mex1 and Mex2 samples because separate stocks were prepared. The same blank values were used across all samples.</p>
<p>-max(DPM-blank or t0,0) implies that if the blank/t0 subtraction resulted in a negative value, 0 was substituted instead.&nbsp;</p>
Specified by the Principal Investigator(s)
Curation Actions Performed on Data
- Loaded data from "Chemoautotrophic assimilation of inorganic carbon_FINAL.xlsx" (sheet 1), using xlsx format with formatting preservation and floating point error adjustment enabled, headers on row 1, blank headers ignored, empty rows removed, and empty string and "nd" values treated as missing; table named 1005988_v1_ETNP_OMZ_AT50_Chemoautotrophic_Assimilation_of_Inorganic_Carbon
- Created new column ISO_Cast_Start_DateTime_UTC by combining Cast Date (UTC) and Cast Time (UTC) columns, parsing with formats %Y-%m-%d and %H:%M:%S respectively, treating input as UTC, and outputting as datetime in format %Y-%m-%dT%H:%M:%SZ, also in UTC
- Renamed columns: "Sample Type" to Sample_Type, "Cast Date (UTC)" to Cast_Date_UTC, "Cast Time (UTC)" to Cast_Time_UTC, "CTD Cast Filename" to CTD_Cast_Filename, "Incubation Duration" to Incubation_Duration, "Raw DPM" to Raw_DPM, "Assimilation_nMC_d^-1" to Assimilation_nMC, "Assimilation_sd_nMC_d^-1" to Assimilation_sd_nMC, "Replicate ID" to Replicate_ID
- Reordered columns to: Depth, Sample_Type, Station, Lat, Long, ISO_Cast_Start_DateTime_UTC, Cast_Date_UTC, Cast_Time_UTC, CTD_Cast_Filename, Incubation Start (local ship time), Incubation Stop (local ship time), Incubation_Duration, Raw_DPM, Assimilation_nMC, Assimilation_sd_nMC, Replicate_ID
- Set column types and formats: Depth and Raw_DPM as integer; Sample_Type, CTD_Cast_Filename, Replicate_ID, Station as string; Lat, Long, Incubation_Duration, Assimilation_nMC, Assimilation_sd_nMC as number; Cast_Date_UTC as date (format %Y-%m-%d); Cast_Time_UTC as time (format %H:%M:%S); ISO_Cast_Start_DateTime_UTC as datetime (format %Y-%m-%dT%H:%M:%SZ); Incubation Start (local ship time) and Incubation Stop (local ship time) as datetime (format %Y-%m-%d %H:%M)
- Updated column metadata with standard name IDs, descriptions, and supplied units for all columns, including marking Depth, ISO_Cast_Start_DateTime_UTC, Lat, and Long as primary parameters
- Dumped final table to CSV output named 1005988_v1_ETNP_OMZ_AT50_Chemoautotrophic_Assimilation_of_Inorganic_Carbon.csv
Curation Actions Performed on Metadata
- Standard metadata entry and text formatting steps were performed.
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Scintillation counter: TRICARBB281000, serial number SGTC05150535
Scintillation counter: TRICARBB281000, serial number SGTC05150535
PI Supplied Instrument Name: Scintillation counter: TRICARBB281000, serial number SGTC05150535 PI Supplied Instrument Description:A scintillation counter was used to measure the radioactivity of ^14C retained on the filters after incubation. Following removal of unassimilated ^14C-bicarbonate, the filters were dried and suspended in Hionic-Fluor scintillation cocktail. The measured radioactivity represented biologically assimilated ^14C and was used to calculate rates of dark carbon assimilation and community chemoautotrophic activity. Measurements were corrected using time-zero controls or blanks and for isotopic fractionation. Instrument Name: Liquid Scintillation Counter Instrument Short Name:LSC Instrument Description: Liquid scintillation counting is an analytical technique which is defined by the incorporation of the radiolabeled analyte into uniform distribution with a liquid chemical medium capable of converting the kinetic energy of nuclear emissions into light energy. Although the liquid scintillation counter is a sophisticated laboratory counting system used to quantify the activity of particulate emitting (ß and a) radioactive samples, it can also detect the auger electrons emitted from 51Cr and 125I samples.
Liquid scintillation counters are instruments assaying alpha and beta radiation by quantitative detection of visible light produced by the passage of rays or particles through a suitable scintillant incorporated into the sample. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB21/
Cruise: AT50-08B
AT50-08B
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
AT50-08B
Maria G. Pachiadaki
Woods Hole Oceanographic Institution
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel