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            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset/1007055.rdf" xlink:actuate="onRequest">Experimental Ocean Carbonate System Controls on Mytilus trossulus Shell Growth from 2023 to 2024</gmx:Anchor>
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            <gco:CharacterString>Cite this dataset as: Gaylord, B., Lewis, M. A., Ninokawa, A. T. (2026) Experimental Ocean Carbonate System Controls on Mytilus trossulus Shell Growth from 2023 to 2024. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-09-04 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/1007055 [access date]</gco:CharacterString>
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        <gco:CharacterString>Ocean Carbonate System Controls on Mytilus trossulus Shell Growth: Experimental Dataset Dataset Description:  Methods and Sampling: &amp;lt;p&amp;gt;Adult Mytilus trossulus (44–75 mm shell length) were collected in September 2023 (n = 131) and December 2023 (n = 73) from Penn Cove Shellfish Farm in Penn Cove, WA, USA. Once they arrived at UC Davis Bodega Marine Lab, they were acclimated for 10 days in flow-through seawater tanks maintained at approximately 13°C prior to experimentation. The following methods are based on and derived from the protocol described in Ninokawa et al. (2024) and Carlson et al. (2025). Experimental incubations were done in a temperature-controlled room at 13°C using airtight 1 L glass vessels containing approximately 0.86 kg seawater and either a live mussel, a de-fleshed and sterilized shell, or only seawater (blank controls to account for any background changes in seawater chemistry).&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;The experiment consisted of three complementary carbonate chemistry manipulation approaches intended to separate the effects of pH, bicarbonate concentration ([HCO3−]), calcium carbonate saturation state (Ω), and calcium concentration ([Ca2+]) on calcification and dissolution rates. To make each treatment, 20 L sumps of filtered seawater were first acidified with Hydrochloric acid (HCl), reducing total alkalinity and shifting dissolved inorganic carbon (DIC) equilibrium toward dissolved CO2. The seawater was then aerated for ~48hrs with Tetra Whisper AP300 Air Pumps to remove excess CO2 through off-gassing. Following aeration, a gradient-based treatment design targeted a continuum of carbonate chemistry conditions, achieved through varying additions of DIC stock solution, HCl, Sodium hydroxide (NaOH), Calcium chloride (CaCl2), or Sodium chloride (NaCl). Carbonate chemistry targets were planned using the R package &amp;lt;em&amp;gt;seacarb&amp;lt;/em&amp;gt;.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;For the first trial, we assessed how pH and DIC influence calcification processes using a batch-treatment design of nine manipulated carbonate chemistry treatments spanning low-to-high pH (7.1–8.2) and [HCO3−] (878.5–4256.8 µmol/kg) conditions, along with one ambient seawater control treatment. For this portion of the experiment, each treatment consisted of a 20L seawater chemistry batch that was distributed among nine replicate live-mussel incubation vessels.&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;A second trial quantified abiotic shell dissolution across a broad calcium carbonate saturation state (aragonite) gradient (0.05–8.24) using de-fleshed mussel shells that had been sterilized by drying at 60°C for approximately 24 h prior to incubation. These shell-only incubations (n = 81) allowed the disaggregation of net calcification from dissolution in order to estimate gross calcification rates (gross calcification = net calcification + dissolution). In contrast to the live-mussel incubations, shell-only incubations isolated abiotic calcium carbonate dissolution from physiological processes such as respiration and ammonium excretion. To increase the magnitude of alkalinity changes associated with shell dissolution, dissolution incubations done in December 2023 and February 2024 were extended to 42–44 h, compared to the 2–4 incubations done in September 2023.&amp;amp;nbsp;&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;In the third trial, we independently manipulated calcium concentration to decouple Ω from pH. This experiment used a 2 × 2 factorial design with two target pH conditions (approximately 7.8 and 8.1) and two calcium treatments: ambient Ω (“low Ca2+”, adjusted with NaCl) and elevated Ω (“high Ca2+”, adjusted with CaCl2). Addition of CaCl2 increased Ω without substantially altering pH to differentiate whether calcification responses were more strongly associated with Ω or pH under similar bicarbonate conditions. Each calcium treatment combination contained six replicate live-mussel incubation vessels.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;For all experiments, seawater chemistry measurements were collected before and after incubations. Measurements included total alkalinity, pH, dissolved oxygen, salinity, temperature, and ammonia concentration; calcium concentration was additionally measured for calcium-manipulation treatments. Initial and final alkalinity were analyzed using a Metrohm robotitrator. A salicylate spectrophotometric assay measured ammonia concentration to correct alkalinity anomaly calculations for any alkalinity changes caused by ammonium production rather than calcification.&amp;amp;nbsp;&amp;lt;/p&amp;gt;</gco:CharacterString>
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        <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/award/886710.rdf" xlink:title="OCE-2129942" xlink:actuate="onRequest">Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2129942 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2129942</gmx:Anchor>
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            http://lod.bco-dmo.org/id/dataset-parameter/1007158.rdf
	Name: mussel_number
	Units: unitless
	Description: &lt;p&gt;Unique ID assigned to each mussel or shell.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/1007159.rdf
	Name: species
	Units: unitless
	Description: &lt;p&gt;Mussel species used in experiment.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/1007160.rdf
	Name: scientific_name
	Units: unitless
	Description: &lt;p&gt;Mussel species used in experiment in scientific name format validated by the WORMS database.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/1007161.rdf
	Name: aphia_id
	Units: unitless
	Description: &lt;p&gt;Aphia ID for the Mussel species used in the experiment, matched from the WORMS database.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/1007162.rdf
	Name: lsid
	Units: unitless
	Description: &lt;p&gt;LSID for the Mussel species used in the experiment, matched from the WORMS database.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/1007163.rdf
	Name: date_cat
	Units: unitless
	Description: &lt;p&gt;Month and year at which experiment was conducted.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/1007164.rdf
	Name: sample_type
	Units: unitless
	Description: &lt;p&gt;Treatments were done on either live&amp;quot; mussels or dissected &amp;quot;shells&amp;quot;. &amp;quot;&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/1007165.rdf
	Name: duration
	Units: hours
	Description: &lt;p&gt;Incubation period.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/1007166.rdf
	Name: sal
	Units: PSU
	Description: &lt;p&gt;Treatment water salinity.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/1007167.rdf
	Name: temp
	Units: degrees Celsius
	Description: &lt;p&gt;Treatment water temperature (measured by Horiba Laqua PC1100).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/1007168.rdf
	Name: gross_calc
	Units: umol/kg/hr
	Description: &lt;p&gt;Gross calcification rate calculated for live&quot; mussels as: ( (-0.5 * (del_ta - del_nh3) * h2o.kg) - calc_diss) /duration/ tissue_mass.&quot;&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/1007169.rdf
	Name: abiotic_diss
	Units: umol/kg/hr
	Description: &lt;p&gt;Abiotic dissolution rate for shells&quot; calculated as (del_ta*(h20.kg)/2/duration/shell_mass).&quot;&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/1007170.rdf
	Name: tissue_mass
	Units: g
	Description: &lt;p&gt;Mass of dried shell tissue.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/1007171.rdf
	Name: shell_mass
	Units: g
	Description: &lt;p&gt;Mass of dried shell.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/1007172.rdf
	Name: TA
	Units: umol/kg
	Description: &lt;p&gt;Average total alkalinity during incubation (measured by Metrohm 855 Robotic Titrosampler).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/1007173.rdf
	Name: pH_t
	Units: unitless
	Description: &lt;p&gt;pH on total scale during incubation (measured by Horiba Laqua PC1100).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/1007174.rdf
	Name: hco3
	Units: umol/kg
	Description: &lt;p&gt;Bicarbonate concentration during incubation (derived from TA and pH using seacarb: Seawater Carbonate Chemistry R package).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/1007175.rdf
	Name: co3
	Units: umol/kg
	Description: &lt;p&gt;Carbonate concentration during incubation (derived from TA and pH using seacarb: Seawater Carbonate Chemistry R package).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/1007176.rdf
	Name: OmegaAragonite
	Units: unitless
	Description: &lt;p&gt;Aragonite saturation state during incubation (derived from TA and pH using seacarb: Seawater Carbonate Chemistry R package).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/1007177.rdf
	Name: OmegaCalcite
	Units: unitless
	Description: &lt;p&gt;Calcite saturation state during incubation (derived from TA and pH using seacarb: Seawater Carbonate Chemistry R package).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/1007178.rdf
	Name: pH_free
	Units: unitless
	Description: &lt;p&gt;Free scale pH during incubation (derived from TA and pH using the seacarb: Seawater Carbonate Chemistry R package).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/1007179.rdf
	Name: H_free
	Units: unitless
	Description: &lt;p&gt;Hydrogen ion concentration (derived from TA and pH using seacarb: Seawater Carbonate Chemistry R package).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/1007180.rdf
	Name: SIR
	Units: unitless
	Description: &lt;p&gt;Substrate Inhibitor Ratio during incubation (derived from TA and pH using seacarb: Seawater Carbonate Chemistry R package).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/1007181.rdf
	Name: co2
	Units: umol/kg
	Description: &lt;p&gt;Carbon dioxide concentration during incubation (derived from TA and pH using seacarb: Seawater Carbonate Chemistry R package).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/1007182.rdf
	Name: pCO2
	Units: uatm
	Description: &lt;p&gt;Partial pressure of carbon dioxide during incubation (derived from TA and pH using seacarb: Seawater Carbonate Chemistry R package).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/1007183.rdf
	Name: DIC
	Units: umol/kg
	Description: &lt;p&gt;Total Dissolved Inorganic Carbons during incubation ([CO2] + [HCO3-] + [CO32-]), (derived from TA and pH using seacarb: Seawater Carbonate Chemistry R package).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/1007184.rdf
	Name: h2o_kg
	Units: kg
	Description: &lt;p&gt;Mass of seawater in individual incubation vessel (derived from TA and pH using seacarb: Seawater Carbonate Chemistry R package).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/1007185.rdf
	Name: del_ta
	Units: umol/kg
	Description: &lt;p&gt;Change in total alkalinity before and after treatment (derived from TA and pH using seacarb: Seawater Carbonate Chemistry R package).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/1007186.rdf
	Name: del_nh3
	Units: umol/kg
	Description: &lt;p&gt;Change in NH3 concentration before and after treatment (derived from TA and pH using seacarb: Seawater Carbonate Chemistry R package).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/1007187.rdf
	Name: calc_diss
	Units: umol/kg
	Description: &lt;p&gt;Correction for alkalinity change due to abiotic dissolution, calculated by dividing CaCO3 dissolution by incubation time and dry shell mass. Only applies to live&quot; mussels. &quot;&lt;/p&gt; 
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                <gco:CharacterString>&amp;lt;p&amp;gt;Adult Mytilus trossulus (44–75 mm shell length) were collected in September 2023 (n = 131) and December 2023 (n = 73) from Penn Cove Shellfish Farm in Penn Cove, WA, USA. Once they arrived at UC Davis Bodega Marine Lab, they were acclimated for 10 days in flow-through seawater tanks maintained at approximately 13°C prior to experimentation. The following methods are based on and derived from the protocol described in Ninokawa et al. (2024) and Carlson et al. (2025). Experimental incubations were done in a temperature-controlled room at 13°C using airtight 1 L glass vessels containing approximately 0.86 kg seawater and either a live mussel, a de-fleshed and sterilized shell, or only seawater (blank controls to account for any background changes in seawater chemistry).&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;The experiment consisted of three complementary carbonate chemistry manipulation approaches intended to separate the effects of pH, bicarbonate concentration ([HCO3−]), calcium carbonate saturation state (Ω), and calcium concentration ([Ca2+]) on calcification and dissolution rates. To make each treatment, 20 L sumps of filtered seawater were first acidified with Hydrochloric acid (HCl), reducing total alkalinity and shifting dissolved inorganic carbon (DIC) equilibrium toward dissolved CO2. The seawater was then aerated for ~48hrs with Tetra Whisper AP300 Air Pumps to remove excess CO2 through off-gassing. Following aeration, a gradient-based treatment design targeted a continuum of carbonate chemistry conditions, achieved through varying additions of DIC stock solution, HCl, Sodium hydroxide (NaOH), Calcium chloride (CaCl2), or Sodium chloride (NaCl). Carbonate chemistry targets were planned using the R package &amp;lt;em&amp;gt;seacarb&amp;lt;/em&amp;gt;.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;For the first trial, we assessed how pH and DIC influence calcification processes using a batch-treatment design of nine manipulated carbonate chemistry treatments spanning low-to-high pH (7.1–8.2) and [HCO3−] (878.5–4256.8 µmol/kg) conditions, along with one ambient seawater control treatment. For this portion of the experiment, each treatment consisted of a 20L seawater chemistry batch that was distributed among nine replicate live-mussel incubation vessels.&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;A second trial quantified abiotic shell dissolution across a broad calcium carbonate saturation state (aragonite) gradient (0.05–8.24) using de-fleshed mussel shells that had been sterilized by drying at 60°C for approximately 24 h prior to incubation. These shell-only incubations (n = 81) allowed the disaggregation of net calcification from dissolution in order to estimate gross calcification rates (gross calcification = net calcification + dissolution). In contrast to the live-mussel incubations, shell-only incubations isolated abiotic calcium carbonate dissolution from physiological processes such as respiration and ammonium excretion. To increase the magnitude of alkalinity changes associated with shell dissolution, dissolution incubations done in December 2023 and February 2024 were extended to 42–44 h, compared to the 2–4 incubations done in September 2023.&amp;amp;nbsp;&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;In the third trial, we independently manipulated calcium concentration to decouple Ω from pH. This experiment used a 2 × 2 factorial design with two target pH conditions (approximately 7.8 and 8.1) and two calcium treatments: ambient Ω (“low Ca2+”, adjusted with NaCl) and elevated Ω (“high Ca2+”, adjusted with CaCl2). Addition of CaCl2 increased Ω without substantially altering pH to differentiate whether calcification responses were more strongly associated with Ω or pH under similar bicarbonate conditions. Each calcium treatment combination contained six replicate live-mussel incubation vessels.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;For all experiments, seawater chemistry measurements were collected before and after incubations. Measurements included total alkalinity, pH, dissolved oxygen, salinity, temperature, and ammonia concentration; calcium concentration was additionally measured for calcium-manipulation treatments. Initial and final alkalinity were analyzed using a Metrohm robotitrator. A salicylate spectrophotometric assay measured ammonia concentration to correct alkalinity anomaly calculations for any alkalinity changes caused by ammonium production rather than calcification.&amp;amp;nbsp;&amp;lt;/p&amp;gt;</gco:CharacterString>
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                <gco:CharacterString>&amp;lt;p&amp;gt;Net calcification rates were determined using the ammonia-corrected alkalinity anomaly technique and normalized to incubation duration, seawater mass (kg), and tissue dry mass (Gazeau et al 2015). Abiotic shell dissolution was quantified from parallel incubations of cleaned, sterilized shells and added to net calcification rates to determine rate of gross calcification. Data processing was done with R statistical software, version 4.3.3. Carbonate system parameters were calculated using the package &amp;lt;em&amp;gt;seacarb &amp;lt;/em&amp;gt;and equilibrium constants from Lueker et al 2000.&amp;lt;/p&amp;gt;</gco:CharacterString>
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                  <gco:CharacterString>* Loaded Mytilus_trossulus_carbonate_decoupling_incubation_data.csv as table res1, using &amp;quot;&amp;quot;, &amp;quot;NA&amp;quot;, and &amp;quot;nd&amp;quot; as missing values.
* Removed a blank-named column.
* Renamed columns for consistency, including mussel.number to mussel_number, h2o.kg to h2o_kg, date.cat to date_cat, and treatment to sample_type.
* Added scientific_name, assigning Mytilus trossulus where applicable.
* Added the WoRMS identifiers for Mytilus trossulus: AphiaID 140482 and LSID urn:lsid:marinespecies.org:taxname:140482. These values were hard-coded because live API lookups were unavailable.
* Reordered the columns into the final specified order.
* Set column data types, including integers for mussel_number and aphia_id, numbers for measurement fields, and strings for descriptive and categorical fields.
* Added metadata—including descriptions, standard names, and units—for all columns. Units included µmol/kg, unitless, degrees C, hours, and g, as applicable.
* Saved the final data file as 1007055_1_data.csv.</gco:CharacterString>
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    <gmd:MD_MaintenanceInformation>
      <gmd:maintenanceAndUpdateFrequency>
        <gmd:MD_MaintenanceFrequencyCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#MD_MaintenanceFrequencyCode" codeListValue="asNeeded" codeSpace="009">asNeeded</gmd:MD_MaintenanceFrequencyCode>
      </gmd:maintenanceAndUpdateFrequency>
      <gmd:maintenanceNote>
        <gco:CharacterString>7.x-1.1</gco:CharacterString>
      </gmd:maintenanceNote>
      <gmd:contact>
        <gmd:CI_ResponsibleParty>
  <gmd:organisationName>
    <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/affiliation/191.rdf" xlink:actuate="onRequest">Biological and Chemical Oceanography Data Management Office (BCO-DMO)</gmx:Anchor>
  </gmd:organisationName>
  <gmd:contactInfo>
    <gmd:CI_Contact>
		  <gmd:phone>
		    <gmd:CI_Telephone>
				  <gmd:voice>
				    <gco:CharacterString>Unavailable</gco:CharacterString>
				  </gmd:voice>
				  <gmd:facsimile>
				    <gco:CharacterString>508-289-2009</gco:CharacterString>
				  </gmd:facsimile>
				</gmd:CI_Telephone>
		  </gmd:phone>
		  <gmd:address>
		    <gmd:CI_Address>
				  <gmd:deliveryPoint>
				    <gco:CharacterString>WHOI MS#36</gco:CharacterString>
				  </gmd:deliveryPoint>
				  <gmd:city>
				    <gco:CharacterString>Woods Hole</gco:CharacterString>
				  </gmd:city>
				  <gmd:administrativeArea>
				    <gco:CharacterString>MA</gco:CharacterString>
				  </gmd:administrativeArea>
				  <gmd:postalCode>
				    <gco:CharacterString>02543</gco:CharacterString>
				  </gmd:postalCode>
				  <gmd:country>
				    <gco:CharacterString>USA</gco:CharacterString>
				  </gmd:country>
				  <gmd:electronicMailAddress>
				    <gco:CharacterString>info@bco-dmo.org</gco:CharacterString>
				  </gmd:electronicMailAddress>
		    </gmd:CI_Address>
		  </gmd:address>
      <gmd:onlineResource>
          <gmd:CI_OnlineResource>
            <gmd:linkage>
              <gmd:URL>http://www.bco-dmo.org</gmd:URL>
            </gmd:linkage>
          </gmd:CI_OnlineResource>
        </gmd:onlineResource>
		  <gmd:hoursOfService>
        <gco:CharacterString>Monday - Friday 8:00am - 5:00pm</gco:CharacterString>
      </gmd:hoursOfService>
		  <gmd:contactInstructions>
		    <gco:CharacterString>For questions regarding this resource, please contact BCO-DMO via the email address provided.</gco:CharacterString>
		  </gmd:contactInstructions>
		</gmd:CI_Contact>
  </gmd:contactInfo>
  <gmd:role>
    <gmd:CI_RoleCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#CI_RoleCode" codeListValue="pointOfContact"  codeSpace="007">pointOfContact</gmd:CI_RoleCode>
  </gmd:role>
</gmd:CI_ResponsibleParty>
      </gmd:contact>
    </gmd:MD_MaintenanceInformation>
  </gmd:metadataMaintenance>
  <gmi:acquisitionInformation>
    <gmi:MI_AcquisitionInformation>
    <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/727.rdf" xlink:title="Optode" xlink:actuate="onRequest">PreSens Microx 4 Micro-optode</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>PreSens Microx 4 Micro-optode</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: PreSens Microx 4 Micro-optode PI Supplied Instrument Description:Oxygen and temperature were measured using a PreSens Microx 4 micro-optode; salinity with a Horiba Laqua PC 1100 conductivity probe; and pH with a Horiba Laqua PC 1100 instrument. Instrument Name: Optode Instrument Short Name:   Instrument Description: An optode or optrode is an optical sensor device that optically measures a specific substance usually with the aid of a chemical transducer.</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/667.rdf" xlink:title="Spectrometer" xlink:actuate="onRequest">Shimadzu Spectrophotometer</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>Shimadzu Spectrophotometer</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Shimadzu Spectrophotometer PI Supplied Instrument Description:To conduct ammonia and pH analysis were done with a Shimadzu Spectrophotometer. Instrument Name: Spectrometer Instrument Short Name:Spectrometer   Instrument Description: A spectrometer is an optical instrument used to measure properties of light over a specific portion of the electromagnetic spectrum. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0460/</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/682.rdf" xlink:title="Titrator" xlink:actuate="onRequest">Metrohm 855 Robotic Titrosampler</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>Metrohm 855 Robotic Titrosampler</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Metrohm 855 Robotic Titrosampler PI Supplied Instrument Description:A Metrohm 855 Robotic Titrosampler, an 800 Dosino, and an 856 Conductivity Module were used in tandem to measure Total Alkalinity before and after treatment.  Instrument Name: Titrator Instrument Short Name:Titrator   Instrument Description: Titrators are instruments that incrementally add quantified aliquots of a reagent to a sample until the end-point of a chemical reaction is reached. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB12/</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      </gmi:MI_AcquisitionInformation>
  </gmi:acquisitionInformation>
</gmi:MI_Metadata>
