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Bacteria and the viruses that infect them (phages) play a major role in marine ecosystems. Their host-virus interactions can alter community structure, preserve genetic diversity, and impact nutrient cycling. Up to a third of the bacteria that dominate marine oxygen minimum zones (OMZs) are infected with phages, yet the consequences of the host-virus interactions are unknown. Environmental DNA sequence data suggest that a dominant lineage of OMZ bacteria (SUP05) is comprised of species and subspecies that carry out different steps in marine nitrogen cycling and that these cells are often infected by viruses. The nitrogen cycling capacity of diverse SUP05 cells can drive the accumulation of nitrogen cycle intermediates in the oceans or lead directly to significant nitrogen loss. The outcome may be driven in part by phages, by causing cell death, metabolic rewiring of infected cells (virocells), or through the expression of phage-encoded auxiliary metabolic genes (AMGs). This project is testing the overarching hypothesis that SUP05 populations are comprised of diverse nitrogen respiring cell types and that their interactions with phages can determine the fate of fixed nitrogen. The investigators are studying SUP05 cells and viruses from OMZs to detail their combined effects on nitrogen cycling and loss processes in OMZs. The project is providing graduate and undergraduate students with hands-on collaborative field and laboratory research experiences, with a focus on broadening participation in oceanography. Through local science festivals, open houses, and interactive classroom lessons, K-12 students are learning about OMZs, nitrogen cycling, and viral infection. Results are being presented at national and international conferences and published in peer-reviewed journals, and all sequence and biogeochemical data, as well as new cultures of SUP05 and phage isolates are being made available to the broader scientific community.&lt;/p&gt;
&lt;p&gt;Up to 25% of the fixed nitrogen in the ocean is lost as gaseous products through denitrification and other microbial processes in OMZs. These regions are also hotspots for nitrous oxide (N2O) production and emission to the atmosphere. While sulfur oxidizing chemoautotrophs from the SUP05 clade of marine bacteria and their viruses are abundant in marine OMZs, the roles of host-virus interactions in biogeochemical processes are largely unknown. This interdisciplinary study is evaluating SUP05 cells and phages in stable OMZ fjord systems using a combination of in situ measurements, DNA and RNA sequencing, and laboratory experiments to link the metabolic activities of SUP05 with the underlying nitrogen transformations they mediate in the ocean. The collective expertise of team members using field approaches to study microbial and viral diversity in nature, cultivation studies to test genomic predictions under controlled laboratory conditions, and isotope analyses to identify the rates and mechanisms underlying microbial nitrogen cycling and loss are being combined to provide new information about the conditions and activities that regulate SUP05 nitrogen cycling and loss in marine OMZs. Ultimately, this multi-faceted approach is filling critical gaps in our understanding of SUP05 cells and phages and how they collectively influence the marine nitrogen cycle.&lt;/p&gt;
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          </gmd:CI_OnlineResource>
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&amp;lt;ul&amp;gt;
&amp;lt;li&amp;gt;Data Conversion&amp;lt;/li&amp;gt;
&amp;lt;li&amp;gt;Align CTD – default settings&amp;lt;/li&amp;gt;
&amp;lt;li&amp;gt;Bottle Summary&amp;lt;/li&amp;gt;
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&amp;lt;li&amp;gt;Derive TEOS-10&amp;lt;/li&amp;gt;
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- Converted Lat and Lon from degrees–decimal minutes to decimal degrees using regex-based parsing
- Standardized the Date column by replacing “Jul” with “July”
- Converted Date from text in the format %B %d %Y to a date field formatted as %Y-%m-%d
- Set column types: Bottle (integer), CStarTr0 (number), Cast (string), Date (date), DepSM (number), FlECO-AFL (number), Lat (number), Lon (number), Ph (number), Potemp090C (number), PrDM (number), Sal00 (number), Sbox0Mm/Kg (number), Station (string), and T090C (number)
- Rounded Lat and Lon to six decimal places, preserving trailing zeros
- Renamed Sbox0Mm/Kg to Sbox0MmKg
- Renamed FlECO-AFL to FlECOAFL
- Updated field metadata for all columns, including descriptions, standard name IDs, supplied units, and primary parameter designations
- Saved the final table as 1008244_v1_sup05titan_processed_ctd_data.csv</gco:CharacterString>
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    <gmi:instrument>
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          <gmi:description>
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      <gmi:instrument>
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                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/1004039.rdf" xlink:title="Sea-Bird SBE 18 pH Sensor" xlink:actuate="onRequest">Sea-Bird SBE 18</gmx:Anchor>
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            <gco:CharacterString>PI Supplied Instrument Name: Sea-Bird SBE 18 PI Supplied Instrument Description:A Sea-Bird SBE 18 sensor, integrated as part of the SBE-911 suite, was used to measure pH. Instrument Name: Sea-Bird SBE 18 pH Sensor Instrument Short Name:SBE 18 pH   Instrument Description: A pH sensor using a pressure-balanced glass-electrode Ag_AgCl-reference pH probe to provide in-situ measurements at depths up to 1200m. The replaceable pH probe is permanently sealed and is supplied with a soaker bottle attachment that prevents the reference electrode from drying out during storage.</gco:CharacterString>
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      <gmi:instrument>
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              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/552.rdf" xlink:title="Sea-Bird SBE 43 Dissolved Oxygen Sensor" xlink:actuate="onRequest">Sea-Bird SBE 43</gmx:Anchor>
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      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
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            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/995549.rdf" xlink:title="Sea-Bird WETLabs ECO FLNTU(RT)D combined fluorometer and turbidity sensor" xlink:actuate="onRequest">WETLabs ECO-FL-RTD</gmx:Anchor>
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            <gco:CharacterString>PI Supplied Instrument Name: WETLabs ECO-FL-RTD PI Supplied Instrument Description:A WETLabs ECO-FL-RTD sensor, integrated as part of the SBE-911 suite, was used to measure fluorescence. Instrument Name: Sea-Bird WETLabs ECO FLNTU(RT)D combined fluorometer and turbidity sensor Instrument Short Name:WET Labs ECO FLNTU(RT)D   Instrument Description: This optical sensor is available in combinations of backscattering, turbidity, and fluorescence measurements. It records in real-time and does not store data. ECOs feature optional active anti-fouling and internal batteries for long-term deployments. This instrument has a user-selectable sample rate up to 8 Hz The fluorometer can typically measure pigment concentrations in the range 0-75 ug/l, with a sensitivity of 0.037 ug/l, at wavelengths of 470 or 695 nm. The turbidity sensor can measure within the range 0-200 NTU, with a sensitivity of 0.098 NTU, at a wavelength of 700 nm. The instrument is stable over a temperature range of 0-30 degC and is rated to a depth of 6000 m.</gco:CharacterString>
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      <gmi:instrument>
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          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: SeaBird C-Star CST-25-DR PI Supplied Instrument Description:A SeaBird C-Star CST-25-DR sensor, integrated as part of the SBE-911 suite, was used to measure beam transmission. Instrument Name: WET Labs {Sea-Bird WETLabs} C-Star transmissometer Instrument Short Name:WL CSTAR Trans   Instrument Description: The C-Star transmissometer has a novel monolithic housing with a highly integrated opto-electronic design to provide a low cost, compact solution for underwater measurements of beam transmittance. The C-Star is capable of free space measurements or flow-through sampling when used with a pump and optical flow tubes. The sensor can be used in profiling, moored, or underway applications. Available with a 6000 m depth rating.

More information on Sea-Bird website: https://www.seabird.com/c-star-transmissometer/product?id=60762467717 Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0160/</gco:CharacterString>
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         <gmx:Anchor xlink:href="https://www.ocean.washington.edu/story/RV_Rachel_Carson" xlink:actuate="onRequest">R/V Rachel Carson (UW)</gmx:Anchor>
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          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/990746.rdf"
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