http://lod.bco-dmo.org/id/dataset/1010479
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2026-10-21
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Growth rates of Trichodesmium erythraeum GBRTRLIN201 and Synechococcus WH8012 from phosphate-manipulation laboratory culture experiments
2026-10-21
publication
2026-10-21
revision
BCO-DMO Linked Data URI
2026-10-21
creation
http://lod.bco-dmo.org/id/dataset/1010479
David A. Hutchins
University of Southern California
principalInvestigator
Feixue Fu
University of Southern California
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Hutchins, D. A., Fu, F. (2026) Growth rates of Trichodesmium erythraeum GBRTRLIN201 and Synechococcus WH8012 from phosphate-manipulation laboratory culture experiments. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-10-21 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/1010479 [access date]
Methods and Sampling: <p><strong>Culturing Methods</strong></p>
<p><em>Synechococcus</em> WH8012 and <em>Trichodesmium erythraeum</em> GBRTRLIN201 were isolated from the Sargasso Sea and the North Great Barrier Reef Lagoon, respectively (Waterbury et al. 1986; Bell et al. 2005). Thermal performance curves were established by stepwise acclimation of stock cultures from 28 °C to target temperatures, with approximately 3–4 days of acclimation between sequential transfers to minimize transient stress responses. <em>Synechococcus</em> was grown from 15 to 34 °C and <em>Trichodesmium</em> from 20 to 34 °C, both at 2–3 °C intervals. Triplicate cultures were grown in 0.2 µm-filtered, microwave-sterilized artificial seawater under a range of phosphate concentrations (0, 0.1, 0.2, 0.4, 0.7, 1, 2, 5, and 10 µM). In addition to phosphate, 100 μM nitrate, 10 μM phosphate, and 125 nM Fe-25 μM EDTA were added. Light was provided at ~40 µmol photons m⁻² s⁻¹ (<em>Synechococcus</em>) and ~150 µmol photons m⁻² s⁻¹ (<em>Trichodesmium</em>) on a 12:12 h light: dark cycle. Cultures were maintained in batch culture, and measurements were conducted at the exponential growth phase.&nbsp;</p>
<p>&nbsp;</p>
<p><strong>Physiological Analyses</strong></p>
<p><em>Growth rate.</em> Daily growth rates were determined from in vivo chlorophyll <em>a</em> fluorescence measured with a Turner Designs 10-AU fluorometer. Specific growth rates were calculated as µ = ln[N(T1)/N(T0)] / (T1– T0). Maximum specific growth rates (µmax) and half-saturation constants (K1/2) were determined by fitting growth rates to the Monod equation.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2149837 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2149837
onGoing
David A. Hutchins
University of Southern California
213-740-5616
Department of Biological Sciences 3616 Trousdale Parkway, AHF 20
Los Angeles
CA
90089
USA
dahutch@usc.edu
pointOfContact
Feixue Fu
University of Southern California
2137403188
Department of Biology, MC 0371, University of Southern California 3616 Trousdale Pkwy
Los Angeles
CA
90089
USA
ffu@usc.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
sampleID
Species
temperature
P_concentration
Specific_growth_rates
Date
Turner Designs 10-AU fluorometer (Turner Designs, San Jose, CA, USA)
theme
None, User defined
sample identification
species scientific name (binomial)
temperature of sample or a generic measurement (not in-situ water temp)
phosphate
growth
date
featureType
BCO-DMO Standard Parameters
Fluorometer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
NSFGEO-NERC: Understanding the consequences of changing phytoplankton elemental use efficiencies for global ocean biogeochemistry
https://osprey.bco-dmo.org/project/895809
NSFGEO-NERC: Understanding the consequences of changing phytoplankton elemental use efficiencies for global ocean biogeochemistry
<p><strong>NSF Award Abstract:</strong><br />
This project is jointly funded by the National Science Foundation's Directorate of Geosciences (NSF/GEO) and the National Environment Research Council (UKRI/NERC) of the United Kingdom (UK) via the NSF/GEO-NERC Lead Agency Agreement. This Agreement allows a single joint US/UK proposal to be submitted and peer-reviewed by the Agency whose investigator has the largest proportion of the budget. Upon successful joint determination of an award, each Agency funds the proportion of the budget and the investigators associated with its own investigators and component of the work.</p>
<p>Earth system computer models that simulate the global cycles of carbon and other elements are one of our most valuable tools to predict environmental change in the future ocean. However, existing modeling methods cannot realistically test how increasing ocean temperatures will affect the needs of marine micro-organisms for the limiting nutrients that usually control their growth, including nitrogen, phosphorus and iron. Because these limiting nutrients govern how much photosynthesis occurs in the ocean, they strongly influence the fate of carbon dioxide produced by human fossil-fuel burning. For this reason, determining how nutrient use by plankton will respond to greenhouse warming of the surface ocean is a priority for scientists and policy makers. This project supports a collaboration between two marine microbiologists in the United States and an Earth system modeler from the United Kingdom that is aiming to better understand how oceanic limiting nutrient cycles and the biological communities they support will react to global warming. The U.S. investigators are using laboratory culture experiments, field work in the coastal ocean, and existing collections of past open ocean chemistry and biology measurements to generate quantitative estimates of rising temperature effects on nutrient elemental use efficiencies (EUEs). An EUE is defined as the rate at which a marine microbe can take up carbon dioxide via photosynthesis, per unit of limiting nutrient in the cell. Preliminary results show that EUEs are highly sensitive to changing temperature, which in turn has far-reaching consequences for how ocean ecosystems function. The U.K. investigator is using these experimentally-determined values to build a new Earth system model that is centered on how warmer conditions will affect EUEs for important groups of marine micro-organisms. This novel approach has the potential to yield much better estimates of the ways that ocean carbon uptake and nutrient cycling will be altered as the ocean continues to warm in the future. Broader Impacts include breaking new scientific ground in oceanography and climate science through improved Earth system models, providing educational opportunities for graduate students, undergraduate researchers, K-12 students, and postdoctoral investigators, and strengthening collaborative ties between the marine science communities of the U.S. and the U.K.</p>
<p>Quantitative relationships between phytoplankton nutrient limitation, primary production and globally-rising sea surface temperatures are still not well understood, largely due to a lack of mechanistic experimental data. This is problematic for Earth system modelers who are trying to project future oceanic productivity levels that underpin predicted changes in ocean ecosystems, information that is urgently needed to inform environmental policy makers. This project addresses this knowledge gap in a closely coordinated, collaborative project between two U.S. marine microbiologists and a biogeochemical modeler from the U.K. The international team of interdisciplinary researchers are applying the emergent concept of Elemental Use Efficiencies (EUEs) to integrate flexible thermal and resource limitation responses into a new generation of Earth system models. EUEs are defined as the amount of carbon fixed per unit time, normalized to the amount of a limiting resource in a phytoplankton cell, as represented by the cellular nutrient quota. Preliminary work suggests that EUEs can be very sensitive to future ocean warning, with major downstream consequences for nutrient-limited marine plankton assemblages and thus also for ocean carbon and nutrient biogeochemistry. The investigators are using high-throughput thermal block methodology to measure EUEs for the limiting nutrients nitrogen, phosphorus and iron in a diverse set of four different functional groups of laboratory phytoplankton isolates across their entire thermal ranges. EUEs obtained for these three key limiting nutrient resources as a function of temperature and degree of nutrient stress are being applied in an iterative manner to inform a novel EUE-centric version of the established NEMO-PISCES ocean model. Field EUE data from coastal and open ocean regimes are being collected for comparison to and ground-truthing of both the laboratory culture results, and the modeling output. This project will advance the discipline of ocean global change biology by providing unique new insights into how biogeochemically-critical marine microbial functional groups may respond to simultaneous shifts in temperature and limiting nutrient resource availability both today, and in the future changing ocean.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
<p><strong>NERC award abstract:</strong><br />
A major focus of biological oceanography is to understand and quantify the effects of temperature and resource availability on phytoplankton productivity and ecosystem dynamics in the world ocean. Classic paradigms like Eppley's temperature curve and the resource response relationships of Liebig and Monod have served the field well, but they fail to capture the full dynamic and interactive nature of resource availability and temperature influences on primary production. This shortcoming is especially problematic for earth system modelers as they urgently try to estimate future oceanic productivity levels that underpin projected changes in the ocean ecosystem, properties that are needed to inform policy makers such as the assessment reports of the IPCC and IPBES. This proposal describes a closely coordinated collaborative project between two U.S. marine microbiologists and a biogeochemical modeler from the U.K. that will apply the emergent concept of Elemental Use Efficiencies (EUEs) to integrate flexible thermal and resource limitation responses into a new generation of earth system models. EUEs are defined as the amount of carbon fixed, per unit of cellular nutrient resource, per unit time. This simple, yet novel concept provides a unique opportunity to integrate the observed consequences of concurrent warming and altered nutrient availability for primary productivity in ocean models to quantify biogeochemical and biological responses in the rapidly changing ocean environment.</p>
EUEs and global ocean biogeochemistry
largerWorkCitation
project
eng; USA
oceans
2025-07-01
2026-04-30
Global
0
BCO-DMO catalogue of parameters from Growth rates of Trichodesmium erythraeum GBRTRLIN201 and Synechococcus WH8012 from phosphate-manipulation laboratory culture experiments
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/1010513.rdf
Name: sampleID
Units: unitless
Description: <p>A unique identifier for each sample, formatted as species code-temperature-phosphate concentration-replicate number. SYN and TRI indicate the species codes; 21, 28, and 33 indicate the experimental temperature in degree C; the phosphate value indicates the experimental phosphate concentration; and 1, 2, and 3 indicate replicate numbers.</p>
http://lod.bco-dmo.org/id/dataset-parameter/1010514.rdf
Name: Species
Units: unitless
Description: <p>Species name</p>
http://lod.bco-dmo.org/id/dataset-parameter/1010515.rdf
Name: temperature
Units: degree C
Description: <p>Experimental temperature</p>
http://lod.bco-dmo.org/id/dataset-parameter/1010516.rdf
Name: P_concentration
Units: micromole
Description: <p>Experimental phosphate concentration</p>
http://lod.bco-dmo.org/id/dataset-parameter/1010517.rdf
Name: Specific_growth_rates
Units: day -1
Description: <p>The specific growth rate under certain experimental condition</p>
http://lod.bco-dmo.org/id/dataset-parameter/1010518.rdf
Name: Date
Units: unitless
Description: <p>Experimental date</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://osprey.bco-dmo.org/dataset/1010479/data/download
download
onLine
dataset
<p><strong>Culturing Methods</strong></p>
<p><em>Synechococcus</em> WH8012 and <em>Trichodesmium erythraeum</em> GBRTRLIN201 were isolated from the Sargasso Sea and the North Great Barrier Reef Lagoon, respectively (Waterbury et al. 1986; Bell et al. 2005). Thermal performance curves were established by stepwise acclimation of stock cultures from 28 °C to target temperatures, with approximately 3–4 days of acclimation between sequential transfers to minimize transient stress responses. <em>Synechococcus</em> was grown from 15 to 34 °C and <em>Trichodesmium</em> from 20 to 34 °C, both at 2–3 °C intervals. Triplicate cultures were grown in 0.2 µm-filtered, microwave-sterilized artificial seawater under a range of phosphate concentrations (0, 0.1, 0.2, 0.4, 0.7, 1, 2, 5, and 10 µM). In addition to phosphate, 100 μM nitrate, 10 μM phosphate, and 125 nM Fe-25 μM EDTA were added. Light was provided at ~40 µmol photons m⁻² s⁻¹ (<em>Synechococcus</em>) and ~150 µmol photons m⁻² s⁻¹ (<em>Trichodesmium</em>) on a 12:12 h light: dark cycle. Cultures were maintained in batch culture, and measurements were conducted at the exponential growth phase.&nbsp;</p>
<p>&nbsp;</p>
<p><strong>Physiological Analyses</strong></p>
<p><em>Growth rate.</em> Daily growth rates were determined from in vivo chlorophyll <em>a</em> fluorescence measured with a Turner Designs 10-AU fluorometer. Specific growth rates were calculated as µ = ln[N(T1)/N(T0)] / (T1– T0). Maximum specific growth rates (µmax) and half-saturation constants (K1/2) were determined by fitting growth rates to the Monod equation.</p>
Specified by the Principal Investigator(s)
<p><strong>Growth rates</strong></p>
<p>Growth rates were calculated from in vivo chlorophyll a fluorescence readings measured with a Turner Designs 10-AU fluorometer using the equation: mu = ln[N(T1)/N(T0)] / (T1 - T0), where N(T0) and N(T1) are fluorescence values at two consecutive time points. Maximum specific growth rates (µmax) and half-saturation constants (K1/2) were determined by fitting growth rates to the Monod equation (Monod, 1949)</p>
Specified by the Principal Investigator(s)
Curation Actions Performed on Data File
- Loaded P curve expt_Growth Rate.csv as table 1010479_v1_lab_cultured_cyanobacteria_growth_rate_data (CSV format, header in row 1; empty rows removed; empty strings and "nd" interpreted as missing values).
- Renamed P concentration (μM) to P_concentration, Specific growth rates (d-1) to Specific_growth_rates, and Date (yyyy-mm-dd) to Date.
- Set Date as a date formatted %Y-%m-%d; P_concentration and Specific_growth_rates as numbers; temperature as an integer; and Species and sampleID as strings.
- Updated column metadata, including descriptions, BCO-DMO standard name IDs, and supplied units, for Date, P_concentration, Species, Specific_growth_rates, sampleID, and temperature.
- Saved the processed file as 1010479_v1_lab_cultured_cyanobacteria_growth_rate_data.csv.
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Turner Designs 10-AU fluorometer (Turner Designs, San Jose, CA, USA)
Turner Designs 10-AU fluorometer (Turner Designs, San Jose, CA, USA)
PI Supplied Instrument Name: Turner Designs 10-AU fluorometer (Turner Designs, San Jose, CA, USA) PI Supplied Instrument Description:Growth rates were calculated from in vivo chlorophyll a fluorescence readings measured with a Turner Designs 10-AU fluorometer using the equation: mu = ln[N(T1)/N(T0)] / (T1 - T0), where N(T0) and N(T1) are fluorescence values at two consecutive time points. Maximum specific growth rates (µmax) and half-saturation constants (K1/2) were determined by fitting growth rates to the Monod equation (Monod, 1949) Instrument Name: Fluorometer Instrument Short Name:Fluorometer Instrument Description: A fluorometer or fluorimeter is a device used to measure parameters of fluorescence: its intensity and wavelength distribution of emission spectrum after excitation by a certain spectrum of light. The instrument is designed to measure the amount of stimulated electromagnetic radiation produced by pulses of electromagnetic radiation emitted into a water sample or in situ. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/113/