http://lod.bco-dmo.org/id/dataset/1010536
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2026-10-22
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Microsphere counts in Dendraster excentricus guts following feeding incubations with microspheres differing in size or functionalized surface
2026-10-22
publication
2026-10-22
revision
Woods Hole Oceanographic Institution (WHOI BCO-DMO)
2026-10-23
publication
https://doi.org/10.26008/1912/bco-dmo.1010536.1
Kelly Rakow Sutherland
University of Oregon
principalInvestigator
Anne W. Thompson
Portland State University
principalInvestigator
Terra C. Hiebert
University of Oregon
principalInvestigator
Darian Madere
Portland State University
principalInvestigator
Carey P. Sweeney
Portland State University
principalInvestigator
Elizabeth Wallace
University of Oregon
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Hiebert, T. C., Sweeney, C. P., Madere, D., Thompson, A. W., Sutherland, K. R., Wallace, E. (2026) Microsphere counts in Dendraster excentricus guts following feeding incubations with microspheres differing in size or functionalized surface. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-10-22 [if applicable, indicate subset used]. doi:10.26008/1912/bco-dmo.1010536.1 [access date]
Dendraster excentricus gut counts Dataset Description: Methods and Sampling: <p>Larval cultures were reared and experimental incubations carried out at the Oregon Institute of Marine Biology from March to April 2025. Experimental feeding incubations using artificial microspheres and natural prey were carried out on 5-day old <em>D. excentricus</em> plutei. Prior to incubations, larvae were removed from their culture container and picked into a small volume (~100 milliliters (mL)) of filtered (0.22 µm) seawater (FSW) using a pulled and flamed Pasteur pipette. After starving briefly (up to 1 hour), 10 larvae were picked into 10-mL glass incubation vials with experimental prey field and allowed to feed for up to 1 hour. We carried out several incubations to investigate selectivity based on prey size and surface. To investigate differential ingestion based on prey size, we used 0.5, 1.0, 3.0, and 10 micrometer (µm) polystyrene microspheres with carboxylate-modified surface and yellow-green (YG) fluorescence (see Table 1 under Supplemental Files). Following the results of prey size, we compared 1.0 µm particles with different surface properties, including polystyrene and silica as well as two different functionalized surfaces; those modified with carboxylate groups or amine groups (Table 1).</p>
<p>Each comparison described above included 3 to 4 replicates with 8 to 10 larvae in each. Incubation chambers were manually inverted to mix every 10 minutes. This concentration of approximately 1 larva per mL incubation is below the known clearance rate of <em>D. excentricus</em> larvae at this age (&lt;1 mL per hour for 10 larvae, Hart 1996). Following incubations, larvae were removed individually by pipetting from incubation chambers, suspended in 1 mL FSW, and similarly fixed in 0.125% glutaraldehyde. All samples were stored in the dark at 4 degrees Celsius (˚C) prior to analysis.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-2419056 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=2419056
completed
Kelly Rakow Sutherland
University of Oregon
541-913-6560
5289 University of Oregon
Eugene
OR
97403-5219
USA
ksuth@uoregon.edu
pointOfContact
Anne W. Thompson
Portland State University
503-725-8052
PO Box 751
Portland
OR
97201
USA
awt@pdx.edu
pointOfContact
Terra C. Hiebert
University of Oregon
5289 University of Oregon
Eugene
OR
97403-5219
USA
terrah@uoregon.edu
pointOfContact
Darian Madere
Portland State University
dmadere@pdx.edu
pointOfContact
Carey P. Sweeney
Portland State University
careypsweeney@gmail.com
pointOfContact
Elizabeth Wallace
University of Oregon
ewal@uoregon.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Incubation
Duration
C_0_5
C_1_0
C_3_0
C_10_0
A_1_0
P_1_0
S_1_0
Nikon D850 SLR
Zeiss LSM 880 laser scanning confocal fluorescence microscope
Nikon Eclipse Ni compound epifluorescent microscope with mounted Nikon D850 SLR
theme
None, User defined
experiment id
duration
number
featureType
BCO-DMO Standard Parameters
Camera
Confocal Laser Scanning Microscope
Fluorescence Microscope
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Are all cell surfaces the same? The effects of particle surface property on predator-prey interactions in the microbial loop
http://www.sutherlandlab.org
Collaborative Research: Are all cell surfaces the same? The effects of particle surface property on predator-prey interactions in the microbial loop
<p><em>NSF Award Abstract:</em><br />
Marine microorganisms are among the most abundant life forms on the planet, playing a key role in ocean nutrient cycling. Though predation on these microorganisms is critical to nutrient cycling, little is known about their interactions with predators - specifically the direct interaction between microorganism cell surfaces and predator capture surfaces. This project examines how cell surfaces may influence the predation of marine microorganisms. Cell surface modification is a recognized strategy for predator avoidance among terrestrial microorganisms, but its application in the ocean is largely unexplored. By examining microbial prey with varying surface characteristics and predators with a range of feeding strategies, this research is providing foundational knowledge for future ocean food web models. This project engages public audiences through exhibits and workshops at museums (e.g., Oregon Museum of Science and Industry) and coastal aquariums with a focus on predator-prey interactions in the ocean from small microbial prey to larger predators. A large-scale art installment emphasizes these food web interactions. These 'Eco Murals' focus on ocean ecosystems and involve participation from community members, especially underrepresented minorities. This project is training the next generation of scientists by involving graduate and undergraduate students in research, professional development, and scientific communication. This research includes independent graduate student research as well as capstone projects in Bioinformatics and Genomics. Undergraduate students participate in this research following the previously successful NSF REU Exploration of Marine Biology on the Oregon Coast model. Finally, by leveraging initiatives aimed at promoting the persistence of historically underrepresented and underserved populations in STEM fields, this project recruits, supports, and retains female, first-generation, and underrepresented minority students.</p>
<p>The differential selection and rejection of microbial prey alters our understanding of carbon fate and nutrient cycling in the ocean. This project directly tests the effects of microbial surface properties on particle selection by globally abundant suspension feeders. Cell surface properties are known to be a fundamental aspect of predation avoidance in terrestrial microbes, but the role of microbial surface properties in avoiding or enhancing predation is a research frontier in ocean science. This knowledge gap limits understanding of microbial mortality, microbial loop function, and prediction of ecosystem response to future climate scenarios. This research links specific particle properties with ecologically-relevant trophic interactions through experiments with widespread suspension feeders representing major feeding strategies by copepod nauplii, pteropods, appendicularians, and echinoderm larvae. First, this project quantifies the surface properties of major marine microbial groups to inform feeding incubations with artificial prey. Second, artificial microspheres with varying surface properties are used in controlled laboratory feeding incubations to determine selectivity and third, to quantify particle fate from released fecal pellets and pseudofeces. Finally, the major marine microbial taxa in the guts of wild-caught suspension feeders are quantified using qPCR. This research forms an integrative approach, yet the results of each objective have scientific impact which can be applied to diverse fields beyond the ocean.</p>
<p>This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.</p>
Surf Props
largerWorkCitation
project
eng; USA
oceans
2025-03-01
2025-04-30
Oregon Institute of Marine Biology, Friday Harbor Labs (FHL), Michael Sars Centre in Bergen, Norway
0
BCO-DMO catalogue of parameters from Microsphere counts in Dendraster excentricus guts following feeding incubations with microspheres differing in size or functionalized surface
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/1010560.rdf
Name: Incubation
Units: unitless
Description: <p>Incubation name for all incubations was alphabetical. Name is unique to entire project</p>
http://lod.bco-dmo.org/id/dataset-parameter/1010561.rdf
Name: Duration
Units: minutes
Description: <p>Incubation duration</p>
http://lod.bco-dmo.org/id/dataset-parameter/1010562.rdf
Name: C_0_5
Units: count per gut
Description: <p>Number of 0.5 µm carboxylate-modified microspheres counted</p>
http://lod.bco-dmo.org/id/dataset-parameter/1010563.rdf
Name: C_1_0
Units: count per gut
Description: <p>Number of 1.0 µm carboxylate-modified microspheres counted</p>
http://lod.bco-dmo.org/id/dataset-parameter/1010564.rdf
Name: C_3_0
Units: count per gut
Description: <p>Number of 3.0 µm carboxylate- modified microspheres counted</p>
http://lod.bco-dmo.org/id/dataset-parameter/1010565.rdf
Name: C_10_0
Units: count per gut
Description: <p>Number of 10.0 µm carboxylate-modified microspheres counted</p>
http://lod.bco-dmo.org/id/dataset-parameter/1010566.rdf
Name: A_1_0
Units: count per gut
Description: <p>Number of 1.0 µm amine- modified microspheres counted</p>
http://lod.bco-dmo.org/id/dataset-parameter/1010567.rdf
Name: P_1_0
Units: count per gut
Description: <p>Number of 1.0 µm unmodified polystyrene microspheres counted</p>
http://lod.bco-dmo.org/id/dataset-parameter/1010568.rdf
Name: S_1_0
Units: count per gut
Description: <p>Number of 1.0 µm unmodified silica microspheres counted</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://doi.org/10.26008/1912/bco-dmo.1010536.1
download
onLine
dataset
<p>Larval cultures were reared and experimental incubations carried out at the Oregon Institute of Marine Biology from March to April 2025. Experimental feeding incubations using artificial microspheres and natural prey were carried out on 5-day old <em>D. excentricus</em> plutei. Prior to incubations, larvae were removed from their culture container and picked into a small volume (~100 milliliters (mL)) of filtered (0.22 µm) seawater (FSW) using a pulled and flamed Pasteur pipette. After starving briefly (up to 1 hour), 10 larvae were picked into 10-mL glass incubation vials with experimental prey field and allowed to feed for up to 1 hour. We carried out several incubations to investigate selectivity based on prey size and surface. To investigate differential ingestion based on prey size, we used 0.5, 1.0, 3.0, and 10 micrometer (µm) polystyrene microspheres with carboxylate-modified surface and yellow-green (YG) fluorescence (see Table 1 under Supplemental Files). Following the results of prey size, we compared 1.0 µm particles with different surface properties, including polystyrene and silica as well as two different functionalized surfaces; those modified with carboxylate groups or amine groups (Table 1).</p>
<p>Each comparison described above included 3 to 4 replicates with 8 to 10 larvae in each. Incubation chambers were manually inverted to mix every 10 minutes. This concentration of approximately 1 larva per mL incubation is below the known clearance rate of <em>D. excentricus</em> larvae at this age (&lt;1 mL per hour for 10 larvae, Hart 1996). Following incubations, larvae were removed individually by pipetting from incubation chambers, suspended in 1 mL FSW, and similarly fixed in 0.125% glutaraldehyde. All samples were stored in the dark at 4 degrees Celsius (˚C) prior to analysis.</p>
Specified by the Principal Investigator(s)
<p>Following our feeding incubations, we directly counted microspheres in the guts of each plutei using a Zeiss LSM 880 laser scanning confocal fluorescence microscope or Nikon Eclipse Ni compound epifluorescent microscope with mounted Nikon D850 SLR camera using GFP, CY3, and DAPI filter cubes. Larvae were triple-rinsed in artificial seawater (ASW) prior to counting to wash away any microspheres sticking to larval epidermis from incubation media. Individuals were mounted between a microscope slide and coverslip using small clay feet, which were compressed gently to make counting individual microspheres easier.</p>
Specified by the Principal Investigator(s)
This section documents curation actions performed prior to publication review with the submitter, and additional information relevant to understanding and reusing this dataset. It distinguishes changes made to the submitted (meta)data from unresolved issues and/or enhancements that improve future reuse and interoperability.
CURATION ACTIONS PERFORMED ON DATA
- Imported original file "Dendraster_Gut_Counts_BCO DMO.xlsx" (sheet 1) into the BCO-DMO data processing system.
- Treated "NA" as a missing data value (NOTE: missing data are empty/blank in the final CSV file).
- Renamed columns to comply with BCO-DMO naming conventions.
- Saved the final file as "1010536_v1_gut_counts.csv".
CURATION ACTIONS PERFORMED ON METADATA
- BCO-DMO's standard metadata entry and text formatting steps were performed. See: https://www.bco-dmo.org/how-to/standard-curation-edits
- Saved Table 1 (originally provided as an embedded table in the Methods & Sampling section) as a PDF and attached it as a Supplemental File.
ISSUES POTENTIALLY IMPACTING REUSE
- N/A
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Nikon D850 SLR
Nikon D850 SLR
PI Supplied Instrument Name: Nikon D850 SLR Instrument Name: Camera Instrument Short Name:camera Instrument Description: All types of photographic equipment including stills, video, film and digital systems. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/311/
Zeiss LSM 880 laser scanning confocal fluorescence microscope
Zeiss LSM 880 laser scanning confocal fluorescence microscope
PI Supplied Instrument Name: Zeiss LSM 880 laser scanning confocal fluorescence microscope Instrument Name: Confocal Laser Scanning Microscope Instrument Short Name: Instrument Description: A laser scanning confocal microscope is a type of confocal microscope that obtains high-resolution optical images with depth selectivity, in which a laser beam passes through a light source aperture and then is focused by an objective lens into a small (ideally diffraction-limited) focal volume within or on the surface of a specimen.
The confocal microscope uses fluorescence optics. 'Confocal' means that the image is obtained from the focal plane only, any noise resulting from sample thickness being removed optically. 'Laser scanning' means the images are acquired point by point under localized laser excitation rather than full sample illumination, as in conventional widefield microscopy.
Nikon Eclipse Ni compound epifluorescent microscope with mounted Nikon D850 SLR
Nikon Eclipse Ni compound epifluorescent microscope with mounted Nikon D850 SLR
PI Supplied Instrument Name: Nikon Eclipse Ni compound epifluorescent microscope with mounted Nikon D850 SLR Instrument Name: Fluorescence Microscope Instrument Short Name: Instrument Description: Instruments that generate enlarged images of samples using the phenomena of fluorescence and phosphorescence instead of, or in addition to, reflection and absorption of visible light. Includes conventional and inverted instruments. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB06/