http://lod.bco-dmo.org/id/dataset/1010596
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2026-10-22
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Dissolved DOC and TDN data from water samples collected at various sites around Puerto Rico during a Sargassum inundation event from May to June 2018
2026-10-22
publication
2026-10-22
revision
BCO-DMO Linked Data URI
2026-10-22
creation
http://lod.bco-dmo.org/id/dataset/1010596
Michael Gonsior
Chesapeake Biological Laboratory
principalInvestigator
Leanne Powers
Chesapeake Biological Laboratory
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Gonsior, M., Powers, L. (2026) Dissolved DOC and TDN data from water samples collected at various sites around Puerto Rico during a Sargassum inundation event from May to June 2018. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2026-10-22 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/1010596 [access date]
Dissolved compound (DOC and TDN) data from water samples collected at various sites around Puerto Rico May-June 2018 during a Sargassum inundation event Dataset Description: Methods and Sampling: <p><strong>Sample collection</strong></p>
<p>Grab water samples (surface, ~0m) were collected dockside or by small boat in in 10-L low-density polyethylene cubitainers that had been pre-cleaned with 0.1 M NaOH for 24 h followed by several rinses of ultrapure Milli-Q water. Within 3 h of collection, samples for DOC and TDN analysis were 0.2-µm filtered through cellulose acetate filters into 40-mL combusted amber glass vials. Single samples for DOC/TDN were collected at each site, unless otherwise noted. The remaining samples were 0.7 µm-filtered through pre-combusted Whatman GF/F glass fiber filters into clean combusted 1-L bottles. SPE samples were collected in duplicate or triplicate. The 0.7 µm-filtered samples were then acidified to pH 2 using ultrapure concentrated HCl and underwent solid phase extraction (SPE). <em>Sargassum</em> exudates were generated by placing fragments of <em>Sargassum</em> in clear 500-mL Teflon bottles with seawater collected on the Atlantic shore with no visible <em>Sargassum</em>. Bottles were placed in a cooler with running water to maintain ambient temperature for 5 h. Seawater in bottles without <em>Sargassum</em> served as a control. After 5 h, exudates and the control were 0.7 µm-filtered as above and also underwent SPE.&nbsp;</p>
<p>&nbsp;</p>
<p><strong>Solid phase extraction (SPE)</strong>&nbsp;</p>
<p>We utilized 1 g Agilent Bond Elut PPL cartridges for SPE. Cartridges were activated with 10 mL ultrapure methanol and rinsed with 10 mL ultrapure 0.1% formic acid water prior to extraction. Samples were then loaded onto cartridges through clean Teflon tubing and extracted by gravity (final extraction volumes ranged from 0.4 to 1.2 L). After extraction, cartridges were rinsed with 30 mL 0.1% formic acid water and dried. Cartridges were then eluted in 10 mL ultrapure MeOH and these methanolic extracts were stored at -20 °C until ICP-MS and FT-ICR MS analysis.</p>
<p>&nbsp;</p>
<p><strong>ICP-MS</strong></p>
<p>Dissolved organic compound (DOS, DOP, DOBr, DOI) analysis followed the methods described in Gonsior et al. (2024) (doi:10.1039/D3VA00361B) for DOS and DOP and in Powers et al. (2024) (doi: 10.1016/j.scitotenv.2023.169292) and Powers et al. (2026) (doi: 10.1029/2025GL119062) for dissolved organic bromine (DOBr) and dissolved organic iodine (DOI). Briefly, methanolic extract aliquots were dried under a gentle stream of nitrogen gas and redissolved in ultrapure water. Samples for DOBr and DOI analysis were also passed through silver cartridges prior to analysis to remove residual halides. DOS and DOP were analyzed by ICP-MS/MS using oxygen as the reaction gas and DOBr and DOI were analyzed by ICP-MS/MS with no gas in the Octopole Reaction System. Final concentrations were corrected for both extraction and dilution volumes. Reported values are the average and standard deviation of three replicate injections per sample.&nbsp;</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1536888 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1536888
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-1536927 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=1536927
onGoing
Michael Gonsior
Chesapeake Biological Laboratory
410-326-7245
146 Williams Street
Solomons
MD
20688-3204
USA
gonsior.michael@gmail.com
pointOfContact
Leanne Powers
Chesapeake Biological Laboratory
315-470-6854
lcpowers@esf.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Sample_ID
Site
location
Date
n
lat_dec
long_dec
depth
DOC
sdev_DOC
TDN
sdev_TDN
Agilent 8900 ICP-QQQ triple-quadrupole inductively coupled plasma mass spectrometer
Bruker Solarix 12 Tesla FT-ICR mass spectrometer
Shimadzu Total Carbon Analyzer (TOC-Vcph)
theme
None, User defined
sample identification
site
site description
date
replicate
latitude
longitude
depth
dissolved organic Carbon
dissolved organic Carbon standard deviation
Total Dissolved Nitrogen
featureType
BCO-DMO Standard Parameters
Mass Spectrometer
Mass Spectrometer
Total Organic Carbon Analyzer
instrument
BCO-DMO Standard Instruments
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Collaborative Research: Phlorotannins - An Important Source of Marine Chromophoric Dissolved Organic Matter?
https://osprey.bco-dmo.org/project/814753
Collaborative Research: Phlorotannins - An Important Source of Marine Chromophoric Dissolved Organic Matter?
<p>NSF Award Abstract:</p>
<p>Chromophoric dissolved organic matter (CDOM), the sunlight absorbing components in filtered water, is important in the study of marine and freshwater ecosystems as it can be used to trace the mixing of surface waters, as a proxy for carbon cycles, and other biogeochemical processes. Although its importance in ocean studies has been firmly established over the last several decades, sources and structural composition of CDOM within the oceans remains unclear and continues to be a subject of debate. Sargassum, a brown alga, is widely distributed in temperate and subtropical marine waters and may be important source of CDOM to the Sargasso Sea and Gulf of Mexico where Sargassum is abundant. This project will investigate the contribution of macro brown algae-derived compounds to the marine CDOM pool. Results from this study will have implications for the marine carbon cycle and satellite remote sensing of ocean color to assess mixing of surface water masses and biogeochemical processes. The project will provide educational opportunities for a postdoctoral scholar, summertime undergraduate internships (through a local NSF-sponsored Research Experiences for Undergraduates (REU) program), and workshop and research opportunities for local high schools students.</p>
<p>Sources of marine CDOM remain debatable and a comprehensive understanding of its origins, distribution and fate have been difficult. Marine CDOM, and in particular the "humic-like" component, have been suggested to originate from terrestrial sources, primarily lignins. However, recent evidence indicates that the exudation of phlorotannins produced by macro brown algae may contribute significantly to the marine CDOM pool. Phlorotannins, a class of polyphenols that are only found in, and continuously exuded by macro brown algae such as Sargassum, strongly absorb ultraviolet light and may have been underestimated in their contribution to the marine CDOM pool within certain geographic locales. Upon partial oxidation, light absorption by these specific compounds extends into longer wavelengths in the visible creating an absorption spectrum similar to that of lignin. These phlorotannins and their transformation products absorb light that might explain in part the "humic-like" signatures observed in open ocean environments. This study aims to characterize the optical properties and molecular composition of Sargassum-derived CDOM including its aerobic oxidation and photochemical behavior, as well as quantify Sargassum-derived CDOM to better estimate its possible contribution to the CDOM pool in the Sargasso Sea and Gulf of Mexico.</p>
Sargassum DOM
largerWorkCitation
project
eng; USA
oceans
-67.3709
-65.5708
17.8476
18.5868
2018-05-30
2018-06-06
Mid-Atlantic Bight (July 2016), Sargasso Sea (July and September 2016), Coastal Bermuda (September/October 2016) and Coastal Puerto Rico (Laguna Grande, Fajardo; Las Croabas, Fajardo; Salinas; May/June 2018)
0
BCO-DMO catalogue of parameters from Dissolved DOC and TDN data from water samples collected at various sites around Puerto Rico during a Sargassum inundation event from May to June 2018
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/1010746.rdf
Name: Sample_ID
Units: unitless
Description: <p>Unique identifier for the collected water or Sargassum exudate sample.</p>
http://lod.bco-dmo.org/id/dataset-parameter/1010747.rdf
Name: Site
Units: unitless
Description: <p>Name of the sampling site where the water sample or exudate was collected.</p>
http://lod.bco-dmo.org/id/dataset-parameter/1010748.rdf
Name: location
Units: unitless
Description: <p>Sampling position relative to the Sargassum mat: at or beneath the mat or mat edge, or approximately 15 m away from the mat.</p>
http://lod.bco-dmo.org/id/dataset-parameter/1010749.rdf
Name: Date
Units: yyyy-mm-dd
Description: <p>Date on which the sample was collected.</p>
http://lod.bco-dmo.org/id/dataset-parameter/1010750.rdf
Name: n
Units: count
Description: <p>Number of replicate measurements associated with the reported concentration and standard deviation.</p>
http://lod.bco-dmo.org/id/dataset-parameter/1010751.rdf
Name: lat_dec
Units: decimal degrees
Description: <p>Latitude of the sampling location in decimal degrees. Positive values indicate north latitude.</p>
http://lod.bco-dmo.org/id/dataset-parameter/1010753.rdf
Name: long_dec
Units: decimal degrees
Description: <p>Longitude of the sampling location in decimal degrees. Negative values indicate west longitude.</p>
http://lod.bco-dmo.org/id/dataset-parameter/1010755.rdf
Name: depth
Units: meters (m)
Description: <p>Depth at which the water sample was collected, in meters. Samples were collected from the surface at approximately 0 m depth.</p>
http://lod.bco-dmo.org/id/dataset-parameter/1010757.rdf
Name: DOC
Units: mg C/L
Description: <p>Dissolved organic carbon concentration measured in the filtered water sample.</p>
http://lod.bco-dmo.org/id/dataset-parameter/1010758.rdf
Name: sdev_DOC
Units: mg C/L
Description: <p>Standard deviation of the replicate DOC measurements.</p>
http://lod.bco-dmo.org/id/dataset-parameter/1010759.rdf
Name: TDN
Units: mg N/L
Description: <p>Total dissolved nitrogen (TDN) concentration measured in the filtered water sample.</p>
http://lod.bco-dmo.org/id/dataset-parameter/1010760.rdf
Name: sdev_TDN
Units: mg N/L
Description: <p>Standard deviation of the replicate TDN measurements.</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
https://osprey.bco-dmo.org/dataset/1010596/data/download
download
onLine
dataset
<p><strong>Sample collection</strong></p>
<p>Grab water samples (surface, ~0m) were collected dockside or by small boat in in 10-L low-density polyethylene cubitainers that had been pre-cleaned with 0.1 M NaOH for 24 h followed by several rinses of ultrapure Milli-Q water. Within 3 h of collection, samples for DOC and TDN analysis were 0.2-µm filtered through cellulose acetate filters into 40-mL combusted amber glass vials. Single samples for DOC/TDN were collected at each site, unless otherwise noted. The remaining samples were 0.7 µm-filtered through pre-combusted Whatman GF/F glass fiber filters into clean combusted 1-L bottles. SPE samples were collected in duplicate or triplicate. The 0.7 µm-filtered samples were then acidified to pH 2 using ultrapure concentrated HCl and underwent solid phase extraction (SPE). <em>Sargassum</em> exudates were generated by placing fragments of <em>Sargassum</em> in clear 500-mL Teflon bottles with seawater collected on the Atlantic shore with no visible <em>Sargassum</em>. Bottles were placed in a cooler with running water to maintain ambient temperature for 5 h. Seawater in bottles without <em>Sargassum</em> served as a control. After 5 h, exudates and the control were 0.7 µm-filtered as above and also underwent SPE.&nbsp;</p>
<p>&nbsp;</p>
<p><strong>Solid phase extraction (SPE)</strong>&nbsp;</p>
<p>We utilized 1 g Agilent Bond Elut PPL cartridges for SPE. Cartridges were activated with 10 mL ultrapure methanol and rinsed with 10 mL ultrapure 0.1% formic acid water prior to extraction. Samples were then loaded onto cartridges through clean Teflon tubing and extracted by gravity (final extraction volumes ranged from 0.4 to 1.2 L). After extraction, cartridges were rinsed with 30 mL 0.1% formic acid water and dried. Cartridges were then eluted in 10 mL ultrapure MeOH and these methanolic extracts were stored at -20 °C until ICP-MS and FT-ICR MS analysis.</p>
<p>&nbsp;</p>
<p><strong>ICP-MS</strong></p>
<p>Dissolved organic compound (DOS, DOP, DOBr, DOI) analysis followed the methods described in Gonsior et al. (2024) (doi:10.1039/D3VA00361B) for DOS and DOP and in Powers et al. (2024) (doi: 10.1016/j.scitotenv.2023.169292) and Powers et al. (2026) (doi: 10.1029/2025GL119062) for dissolved organic bromine (DOBr) and dissolved organic iodine (DOI). Briefly, methanolic extract aliquots were dried under a gentle stream of nitrogen gas and redissolved in ultrapure water. Samples for DOBr and DOI analysis were also passed through silver cartridges prior to analysis to remove residual halides. DOS and DOP were analyzed by ICP-MS/MS using oxygen as the reaction gas and DOBr and DOI were analyzed by ICP-MS/MS with no gas in the Octopole Reaction System. Final concentrations were corrected for both extraction and dilution volumes. Reported values are the average and standard deviation of three replicate injections per sample.&nbsp;</p>
Specified by the Principal Investigator(s)
<p>Data processing details have not been detailed by the dataset author, please refer to the methods desription.</p>
Specified by the Principal Investigator(s)
Curation Actions Performed on Data File
- Loaded PuertoRico_Sargassum_DOC_TDN.xlsx (XLSX, sheet 1, header row 1, formatting preserved) as table 1010596_v1_sargassum_inundation_event_doc_and_tdn_data; empty strings and “NA” were treated as missing values.
- Set column types: DOC, TDN, sdev_DOC, sdev_TDN, lat_dec, and long_dec as numbers; depth and n as integers; Sample_ID, Site, and location as strings; and Date as a date formatted %Y-%m-%d.
- Updated column metadata, including BCO-DMO descriptions, standard names, and supplied units, for DOC, TDN, sdev_DOC, sdev_TDN, Date, Sample_ID, Site, location, n, depth, lat_dec, and long_dec. Depth, lat_dec, and long_dec were flagged as primary parameters.
- Saved the final table as 1010596_v1_sargassum_inundation_event_doc_and_tdn_data.csv.
Specified by BCO-DMO Data Managers
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Agilent 8900 ICP-QQQ triple-quadrupole inductively coupled plasma mass spectrometer
Agilent 8900 ICP-QQQ triple-quadrupole inductively coupled plasma mass spectrometer
PI Supplied Instrument Name: Agilent 8900 ICP-QQQ triple-quadrupole inductively coupled plasma mass spectrometer PI Supplied Instrument Description:We utilized 1 g Agilent Bond Elut PPL cartridges for SPE. Cartridges were activated with 10 mL ultrapure methanol and rinsed with 10 mL ultrapure 0.1% formic acid water prior to extraction. Samples were then loaded onto cartridges through clean Teflon tubing and extracted by gravity (final extraction volumes ranged from 0.4 to 1.2 L). Instrument Name: Mass Spectrometer Instrument Short Name:Mass Spec Instrument Description: General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Bruker Solarix 12 Tesla FT-ICR mass spectrometer
Bruker Solarix 12 Tesla FT-ICR mass spectrometer
PI Supplied Instrument Name: Bruker Solarix 12 Tesla FT-ICR mass spectrometer PI Supplied Instrument Description:Mass spectrometric analyses were undertaken at the Helmholtz Zentrum, Munich, Germany using a Bruker Solarix 12 Tesla Fourier transform ion cyclotron resonance mass spectrometer (FT-ICR MS) using negative electrospray ionization at -3600 V. The flow rate was 120 µL/h, 500 spectra were averaged. Pre-calibration was performed with arginine clusters and spectra were post-calibrated in the Bruker Data Analysis software using known DOM m/z ions across the m/z range of 200-700, with signal-to-noise set to 10. Instrument Name: Mass Spectrometer Instrument Short Name:Mass Spec Instrument Description: General term for instruments used to measure the mass-to-charge ratio of ions; generally used to find the composition of a sample by generating a mass spectrum representing the masses of sample components. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB16/
Shimadzu Total Carbon Analyzer (TOC-Vcph)
Shimadzu Total Carbon Analyzer (TOC-Vcph)
PI Supplied Instrument Name: Shimadzu Total Carbon Analyzer (TOC-Vcph) PI Supplied Instrument Description:DOC and TDN concentrations were measured using a Shimadzu TOC-V. Instrument Name: Total Organic Carbon Analyzer Instrument Short Name:TOC analyzer Instrument Description: A unit that accurately determines the carbon concentrations of organic compounds typically by detecting and measuring its combustion product (CO2). See description document at: http://bcodata.whoi.edu/LaurentianGreatLakes_Chemistry/bs116.pdf Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB04/