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        <gco:CharacterString>Zooplankton counts (density) Dataset Description: &amp;lt;h3&amp;gt;Zooplankton Densities/Abundance&amp;lt;/h3&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Principal Investigator:&amp;lt;/strong&amp;gt; Lewis S. Incze &amp;lt;a href=&amp;quot;mailto:lincze@usm.maine.edu&amp;quot;&amp;gt;(lincze@usm.maine.edu)&amp;lt;/a&amp;gt;&amp;lt;br /&amp;gt;
&amp;lt;strong&amp;gt;Research Technicians:&amp;lt;/strong&amp;gt; Ford Dye, Beth Novak, Nicholas Wolff&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Methods:&amp;lt;/strong&amp;gt;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Using a pumping system appended to the CTD unit, measured water volumes (typically 40 liters) were sampled at descrete depths and filtered through 40 micron mesh prior to sorting. A subsample of the filtered sample was used for the zooplankton counts and identification. The size of the subsample varied and depended on the amount of zooplankton present - subsamples were larger when there were fewer animals.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;From the subsamples, all zooplankton were counted and identifed. Counts were then converted into number per cubic meter as follows: Number counted / (Subsample size X Amount filtered).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;It is worth noting that the frequency of repeated densities is an artifact of the small subsample volumes screened in order to process a large number of samples. The small sample volumes did not give us the ability to resolve small differences in abundance at individual species and stages (e.g., Cal fin Nauplius I). At this level of sorting many counts were reported as zeros, ones, twos or threes. These counts are at the threshold for detection, and the results give the false impression of many identical densities (concentrations). This also shows up in gaps in the life histories as well. For example, a single depth/station might have NI and NIII and NIV stages, but not II or V. As a result, samples were often recombined to give the population characteristics of an integrated water column, or a group of samples. The approach worked well for our research objectives and we are well aware of its limitations. A single station and depth in our data can be deceiving and does not tell the whole story.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Note:&amp;lt;/strong&amp;gt; Sampling for cruise EL9904, events EL10999.24 and EL111299.6 are intentional replication sites. All sampling was made at same depth level. These stations were used to test the repeatability of methods.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Cruises&amp;lt;/strong&amp;gt;&amp;lt;/p&amp;gt;

&amp;lt;ul&amp;gt;
&amp;lt;li&amp;gt;SJ9508 (Seward Johnson Cruise 9508 to Southwest Georges Bank, 6 - 16 June 1995)&amp;lt;/li&amp;gt;
&amp;lt;li&amp;gt;OC303 (Oceanus Cruise 303 to Georges Bank, 6 - 23 May 1997)&amp;lt;/li&amp;gt;
&amp;lt;li&amp;gt;EL9904 (Edwin Link Cruise 9904 to Georges Bank, 14 - 28 April 1999)&amp;lt;/li&amp;gt;
&amp;lt;li&amp;gt;EL9905 (Edwin Link Cruise 9905 to Georges Bank, 10 - 29 May 1999)&amp;lt;/li&amp;gt;
&amp;lt;/ul&amp;gt;

&amp;lt;p&amp;gt;&amp;amp;nbsp;&amp;lt;/p&amp;gt;

&amp;lt;pre&amp;gt;
&amp;lt;strong&amp;gt; Any questions, contact:&amp;lt;/strong&amp;gt;

Lewis S. Incze
University of Southern Maine
P.O. Box 9300
96 Falmouth Street
Portland, ME 04104-9300

Voice:  207 228 8070
Fax:    207 228 8057
Email:  &amp;lt;a href=&amp;quot;mailto:lincze@usm.maine.edu&amp;quot;&amp;gt;(lincze@usm.maine.edu)&amp;lt;/a&amp;gt;

&amp;lt;em&amp;gt; updated:  23 November 2004; gfh&amp;lt;/em&amp;gt;
&amp;lt;/pre&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt;Using a pumping system appended to the CTD unit, measured water volumes (typically 40 liters) were sampled at descrete depths and filtered through 40 micron mesh prior to sorting. A subsample of the filtered sample was used for the zooplankton counts and identification. The size of the subsample varied and depended on the amount of zooplankton present - subsamples were larger when there were fewer animals.&amp;lt;/p&amp;gt;</gco:CharacterString>
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from Cruise: SJ9508 Using a pumping system appended to the CTD unit, measured water volumes (typically 40 liters) were sampled at descrete depths and filtered through 40 micron mesh prior to sorting. A subsample of the filtered sample was used for the zooplankton counts and identification. The size of the subsample varied and depended on the amount of zooplankton present - subsamples were larger when there were fewer animals.

from Cruise: OC303 Using a pumping system appended to the CTD unit, measured water volumes (typically 40 liters) were sampled at descrete depths and filtered through 40 micron mesh prior to sorting. A subsample of the filtered sample was used for the zooplankton counts and identification. The size of the subsample varied and depended on the amount of zooplankton present - subsamples were larger when there were fewer animals.

from Cruise: EL9904 Using a pumping system appended to the CTD unit, measured water volumes (typically 40 liters) were sampled at descrete depths and filtered through 40 micron mesh prior to sorting. A subsample of the filtered sample was used for the zooplankton counts and identification. The size of the subsample varied and depended on the amount of zooplankton present - subsamples were larger when there were fewer animals.

from Cruise: EL9905 Pump samples were taken by attaching one end of a 5 cm x 60 m reinforced hose to the CTD/rosette frame so that the hose opening was near (within ~0.25 m of) the CTD sensors. The CTD was lowered to depth (usually 50 m) and stopped at discrete sampling depths at 5 m intervals up to a depth of 5 m. Time was given for the system to clear at each new depth before sampling. A gas-powered diaphragm pump delivered water from sampling depths to the surface at a nominal rate of 0.3 m3/min. This water passed into a small, rapidly draining reservoir (0.13 m3) on deck to dampen the surge. This reservoir also was drained between sampling depths. A 1.9 cm ID hose carried water from the reservoir to individual samplers equipped with 40 um mesh nets. An electronic timer and flow meter installed in the 1.9 cm hose was started and stopped for each sample, providing very high accuracy measurements of the volumes filtered. The final sampling rate averaged 13 l/min, and most samples were filtered from 27-33 l of water. Samples were preserved in 3-5% buffered formalin.</gco:CharacterString>
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&amp;lt;p&amp;gt;It is worth noting that the frequency of repeated densities is an artifact of the small subsample volumes screened in order to process a large number of samples. The small sample volumes did not give us the ability to resolve small differences in abundance at individual species and stages (e.g., Cal fin Nauplius I). At this level of sorting many counts were reported as zeros, ones, twos or threes. These counts are at the threshold for detection, and the results give the false impression of many identical densities (concentrations). This also shows up in gaps in the life histories as well. For example, a single depth/station might have NI and NIII and NIV stages, but not II or V. As a result, samples were often recombined to give the population characteristics of an integrated water column, or a group of samples. The approach worked well for our research objectives and we are well aware of its limitations. A single station and depth in our data can be deceiving and does not tell the whole story.&amp;lt;/p&amp;gt;

from Cruise: SJ9508 From the subsamples, all zooplankton were counted and identifed. Counts were then converted into number per cubic meter as follows: Number counted / (Subsample size X Amount filtered).
&lt;p&gt;
It is worth noting that the frequency of repeated densities is an artifact of the small 
subsample volumes screened in order to process a large number of samples.  The small sample
volumes did not give us the ability to resolve small differences in abundance at individual
species and stages (e.g., Cal fin Nauplius I).  At this level of sorting many counts were 
reported as zeros, ones, twos or threes.  These counts are at the threshold for detection, 
and the results give the false impression of many identical densities (concentrations).
This also shows up in gaps in the life histories as well.  For example, a single depth/station
might have NI and NIII and NIV stages, but not II or V.  As a result, samples were often 
recombined to give the population characteristics of an integrated water column, or a group of
samples. The approach worked well for our research objectives and we are well aware of its 
limitations. A single station and depth in our data can be deceiving and does not tell the 
whole story.
&lt;/p&gt;

from Cruise: OC303 From the subsamples, all zooplankton were counted and identifed. Counts were then converted into number per cubic meter as follows: Number counted / (Subsample size X Amount filtered).
&lt;p&gt;
It is worth noting that the frequency of repeated densities is an artifact of the small 
subsample volumes screened in order to process a large number of samples.  The small sample
volumes did not give us the ability to resolve small differences in abundance at individual
species and stages (e.g., Cal fin Nauplius I).  At this level of sorting many counts were 
reported as zeros, ones, twos or threes.  These counts are at the threshold for detection, 
and the results give the false impression of many identical densities (concentrations).
This also shows up in gaps in the life histories as well.  For example, a single depth/station
might have NI and NIII and NIV stages, but not II or V.  As a result, samples were often 
recombined to give the population characteristics of an integrated water column, or a group of
samples. The approach worked well for our research objectives and we are well aware of its 
limitations. A single station and depth in our data can be deceiving and does not tell the 
whole story.
&lt;/p&gt;

from Cruise: EL9904 From the subsamples, all zooplankton were counted and identifed. Counts were then converted into number per cubic meter as follows: Number counted / (Subsample size X Amount filtered).
&lt;p&gt;
It is worth noting that the frequency of repeated densities is an artifact of the small 
subsample volumes screened in order to process a large number of samples.  The small sample
volumes did not give us the ability to resolve small differences in abundance at individual
species and stages (e.g., Cal fin Nauplius I).  At this level of sorting many counts were 
reported as zeros, ones, twos or threes.  These counts are at the threshold for detection, 
and the results give the false impression of many identical densities (concentrations).
This also shows up in gaps in the life histories as well.  For example, a single depth/station
might have NI and NIII and NIV stages, but not II or V.  As a result, samples were often 
recombined to give the population characteristics of an integrated water column, or a group of
samples. The approach worked well for our research objectives and we are well aware of its 
limitations. A single station and depth in our data can be deceiving and does not tell the 
whole story.
&lt;/p&gt;

from Cruise: EL9905 From the subsamples, all zooplankton were counted and identifed. Counts were then converted into number per cubic meter as follows: Number counted / (Subsample size X Amount filtered).
&lt;p&gt;
It is worth noting that the frequency of repeated densities is an artifact of the small 
subsample volumes screened in order to process a large number of samples.  The small sample
volumes did not give us the ability to resolve small differences in abundance at individual
species and stages (e.g., Cal fin Nauplius I).  At this level of sorting many counts were 
reported as zeros, ones, twos or threes.  These counts are at the threshold for detection, 
and the results give the false impression of many identical densities (concentrations).
This also shows up in gaps in the life histories as well.  For example, a single depth/station
might have NI and NIII and NIV stages, but not II or V.  As a result, samples were often 
recombined to give the population characteristics of an integrated water column, or a group of
samples. The approach worked well for our research objectives and we are well aware of its 
limitations. A single station and depth in our data can be deceiving and does not tell the 
whole story.
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            </gmi:type>
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            <gmi:platform>
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         <gmx:Anchor xlink:href="http://vocab.nerc.ac.uk/collection/C17/current/33SW" xlink:actuate="onRequest">Community Standard Description</gmx:Anchor>
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    </gmi:citation>
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          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54000.rdf"
           xlink:title="33SW" xlink:actuate="onRequest">R/V Seward Johnson</gmx:Anchor>
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                          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/person/50433.rdf" xlink:actuate="onRequest">Dr Neil Oakey</gmx:Anchor>
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            </gmi:MI_Operation>
      </gmi:operation><gmi:operation>
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            <gmi:description>
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              </gmd:MD_Identifier>
            </gmi:identifier>
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            </gmi:type>
            <gmi:parentOperation gco:nilReason="inapplicable"/>
            <gmi:platform>
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       <gmd:title>
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       <gmd:date gco:nilReason="unknown"/>
     </gmd:CI_Citation>
    </gmi:citation>
    <gmi:citation>
     <gmd:CI_Citation>
       <gmd:title>
         <gmx:Anchor xlink:href="http://vocab.nerc.ac.uk/collection/C17/current/32OC" xlink:actuate="onRequest">Community Standard Description</gmx:Anchor>
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       <gmd:date gco:nilReason="unknown"/>
     </gmd:CI_Citation>
    </gmi:citation>
    <gmi:identifier>
      <gmd:MD_Identifier>
        <gmd:authority>
          <gmd:CI_Citation>
            <gmd:title>
              <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/authority/1.rdf" xlink:actuate="onRequest">International Council for the Exploration of the Sea</gmx:Anchor>
            </gmd:title>
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          </gmd:CI_Citation>
        </gmd:authority>
        <gmd:code>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/53997.rdf"
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        </gmd:code>
      </gmd:MD_Identifier>
    </gmi:identifier>
    <gmi:description>
      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/53997.rdf" xlink:title="R/V Oceanus" xlink:actuate="onRequest">vessel</gmx:Anchor>
    </gmi:description>
    <gmi:instrument gco:nilReason="unknown"/>
  </gmi:MI_Platform>
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            <gmi:plan>
              <gmi:MI_Plan>
                <gmi:status>
                  <gmd:MD_ProgressCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#MD_ProgressCode" codeListValue="completed"/>
                </gmi:status>
                <gmi:citation>
                  <gmd:CI_Citation>
                    <gmd:title>
                      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/deployment/57449.rdf" xlink:title="Cruise" xlink:actuate="onRequest">OC303</gmx:Anchor>
                    </gmd:title>
                    <gmd:date gco:nilReason="unknown"/>
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                      <gmd:CI_ResponsibleParty>
                      <gmd:individualName>
                          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/person/50424.rdf" xlink:actuate="onRequest">Dr Greg Lough</gmx:Anchor>
                        </gmd:individualName>
                        <gmd:organisationName>
                          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/affiliation/206.rdf" xlink:title="Affiliation" xlink:actuate="onRequest">Northeast Fisheries Science Center - Woods Hole</gmx:Anchor>
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                                <gmd:URL>http://globec.whoi.edu/globec-dir/reports/oc303/oc303.html</gmd:URL>
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                </gmi:citation>
              </gmi:MI_Plan>
            </gmi:plan>
            </gmi:MI_Operation>
      </gmi:operation><gmi:operation>
          <gmi:MI_Operation>
            <gmi:description>
              <gco:CharacterString>Cruise: EL9904</gco:CharacterString>
            </gmi:description>
            <gmi:identifier>
              <gmd:MD_Identifier>
                <gmd:code>
                  <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/deployment/57394.rdf" xlink:title="Cruise" xlink:actuate="onRequest">EL9904</gmx:Anchor>
                </gmd:code>
              </gmd:MD_Identifier>
            </gmi:identifier>
            <gmi:status>
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            </gmi:status>
            <gmi:type>
              <gmi:MI_OperationTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MI_OperationTypeCode" codeListValue="real"/>
            </gmi:type>
            <gmi:parentOperation gco:nilReason="inapplicable"/>
            <gmi:platform>
  <gmi:MI_Platform>
    <gmi:citation>
     <gmd:CI_Citation>
       <gmd:title>
         <gmx:Anchor xlink:href="http://vocab.nerc.ac.uk/collection/C17/current/33EL" xlink:actuate="onRequest">Community Standard Description</gmx:Anchor>
       </gmd:title>
       <gmd:date gco:nilReason="unknown"/>
     </gmd:CI_Citation>
    </gmi:citation>
    <gmi:identifier>
      <gmd:MD_Identifier>
        <gmd:authority>
          <gmd:CI_Citation>
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            </gmd:title>
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          </gmd:CI_Citation>
        </gmd:authority>
        <gmd:code>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/53991.rdf"
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    </gmi:identifier>
    <gmi:description>
      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/53991.rdf" xlink:title="R/V Edwin Link" xlink:actuate="onRequest">vessel</gmx:Anchor>
    </gmi:description>
    <gmi:instrument gco:nilReason="unknown"/>
  </gmi:MI_Platform>
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                      <gmd:individualName>
                          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/person/50413.rdf" xlink:actuate="onRequest">Dr Lewis Incze</gmx:Anchor>
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                          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/affiliation/94.rdf" xlink:title="Affiliation" xlink:actuate="onRequest">Bigelow Laboratory for Ocean Sciences</gmx:Anchor>
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      </gmi:operation><gmi:operation>
          <gmi:MI_Operation>
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              <gmd:MD_Identifier>
                <gmd:code>
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          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/53991.rdf"
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                          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/person/50424.rdf" xlink:actuate="onRequest">Dr Greg Lough</gmx:Anchor>
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          <gmi:platform>
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         <gmx:Anchor xlink:href="https://ceoas.oregonstate.edu/oceanus/" xlink:actuate="onRequest">R/V Oceanus</gmx:Anchor>
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    <gmi:citation>
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