<div><p>Sampling methods for recovery of chlorophyll <em>a</em> and bacteria from sea ice cores follows those described in:<br />
Garrison, D.L. and K.R. Buck(1986), Organism losses during ice melting: a serious bias in sea ice community studies. <em>Polar Biol.</em>, <strong>6</strong>:237-239.</p>
<p>Recommendations for reporting were used as outlined by:<br />
Horner, R. <em>et al.</em>,(1992), Ecology of Sea Ice Biota. I: Habitat, Terminology and Methodology. <em> Polar Biol.</em> <strong>12</strong>:417-427</p>
<pre>
# Ice core bacteria data from Southern Ocean GLOBEC
# C. Fritsen and F. Stewart
# * BactAbun = bacterial abundance = cells m-3
# * BactBio = bacterial biomass = mg C m-3 = ug C l-1
# * chla = mg chla m-3 = ug chla l-1
# * sect_top_depth = depth(m) at top of core section
</pre></div>
Ice core bacteria data
<div><h2>Bacteria Abundance, Biomass and Chlorophyll <em>a</em> in Ice Cores</h2>
<p><strong>NOTES:</strong><br />
NBP0104: Cores labelled with "DNA" were collected for DNA analysis.</p>
<pre>
<strong>Contributor:</strong>
Dr. Christian Fritsen
University and Community College System of Nevada
Desert Research Institute
Div. of Earth and Ecosystem Sciences
2215 Raggio Parkway
Reno, NV 89512
Office: 775/673-7487
</pre><h3>BG 235 - Methods used for chlorophyll <em>a</em> (chla) analysis and bacteria biomass determination</h3>
<p><strong>Core Sampling techniques:</strong></p>
<p>Sampling methods for recovery of chlorophyll <em>a</em> and bacteria from sea ice cores follows those described in:<br />
Garrison, D.L. and K.R. Buck(1986), Organism losses during ice melting: a serious bias in sea ice community studies. <em>Polar Biol.</em>, <strong>6</strong>:237-239.</p>
<p>Recommendations for reporting were used as outlined by:<br />
Horner, R. <em>et al.</em>,(1992), Ecology of Sea Ice Biota. I: Habitat, Terminology and Methodology. <em> Polar Biol.</em> <strong>12</strong>:417-427</p>
<p><strong>Analytic Techniques:</strong></p>
<p>Chla (mg m<sup>-3</sup>):</p>
<ul><li>determined fluorometrically (Turner Designs 10AU Fluorometer) following extraction in 90% acetone (Parsons <em>et al</em>., 1984)</li>
<li>ice core chla corrected to account for chla in filtered sea water (FSW) added to core sections during melting</li>
</ul><p>Bacteria cell abundance (cells m<sup>-3</sup>) and biomass (mg C m<sup>-3</sup>):</p>
<p>LMG 0106</p>
<ul><li>preserved (0.5% glutaraldehyde) samples stained with 4',6-diamidino-2-phenylindole (DAPI; 0.1 to 0.3% final concentration), filtered through 0.2 mm black, polycarbonate membrane filters, and mounted onto glass microscope slides on the ship (within 24 hours following collection)</li>
<li>bacteria enumerated using epifluorescence microscopy and sized using digital images taken with Image Pro Plus</li>
<li>bacteria biomass determined using cell abundance, cell biovolume (BV; mm<sup>3</sup>; as determined from mean length and width measurements), and an allometric conversion factor for bacterial carbon per volume specific for DAPI-stained bacteria (cellular carbon = 218 X BV<sup>0.86</sup>; Loferer-Kribacher <em>et al.</em>, 1998).</li>
<li>ice core samples corrected for FSW dilution</li>
</ul><p>NBP 0104</p>
<ul><li>preserved (0.5% glutaraldehyde) samples stained with Sybri Gold (0.01% final concentration), filtered through 0.2 mm Anodisc filters (Whatman), and mounted onto glass microscope slides at home institution (~1-2 months following collection)</li>
<li>bacteria enumerated using epifluorescence microscopy and sized using digital images taken with Image Pro Plus</li>
<li>bacteria biomass determined using cell abundance, cell biovolume (BV; mm<sup>3</sup>), and an allometric conversion factor for bacterial carbon per volume specific for Acridine Orange-stained bacteria (cellular carbon = 89.9 X BV<sup>0.59</sup>; Simon and Azam, 1989). Note: an AO-specific carbon per volume conversion factor was used in calculating biomass in Sybri Gold-stained samples because both AO and Sybri Gold stain bacteria cells similarly relative to DAPI (unpublished data).</li>
<li>ice core samples corrected for FSW dilution</li>
</ul><p>Loferer-Kribacher, M., Klima, J., and R. Psenner. 1998. Determination of bacterial cell dry mass by transmission electron microscopy and densitometric image analysis. Applied and Environmental Microbiology. 64:688-694.</p>
<p>Parsons,T.R., Maita, Y., and C.M. Lalli. 1984. A manual of chemical and biological methods for seawater analysis. Pergamon Press. Elmsford, New York.</p>
<p>Simon, M., and F. Azam. 1989. Protein content and protein synthesis rates of planktonic marine bacteria. Marine Ecology Progress Series. 51, 201-213.</p>
<p><em>updated: April 20, 2006</em></p></div>
bacteria_ice
<div><p>Sampling methods for recovery of chlorophyll <em>a</em> and bacteria from sea ice cores follows those described in:<br />
Garrison, D.L. and K.R. Buck(1986), Organism losses during ice melting: a serious bias in sea ice community studies. <em>Polar Biol.</em>, <strong>6</strong>:237-239.</p>
<p>Recommendations for reporting were used as outlined by:<br />
Horner, R. <em>et al.</em>,(1992), Ecology of Sea Ice Biota. I: Habitat, Terminology and Methodology. <em> Polar Biol.</em> <strong>12</strong>:417-427</p>
<p>LMG 0106</p>
<ul><li>preserved (0.5% glutaraldehyde) samples stained with 4',6-diamidino-2-phenylindole (DAPI; 0.1 to 0.3% final concentration), filtered through 0.2 mm black, polycarbonate membrane filters, and mounted onto glass microscope slides on the ship (within 24 hours following collection)</li>
<li>bacteria enumerated using epifluorescence microscopy and sized using digital images taken with Image Pro Plus</li>
<li>bacteria biomass determined using cell abundance, cell biovolume (BV; mm<sup>3</sup>; as determined from mean length and width measurements), and an allometric conversion factor for bacterial carbon per volume specific for DAPI-stained bacteria (cellular carbon = 218 X BV<sup>0.86</sup>; Loferer-Kribacher <em>et al.</em>, 1998).</li>
<li>ice core samples corrected for FSW dilution</li>
</ul><pre>
# LMG0106: "a" "b" "c" labels appended to core numbers denote replicate cores.
# "(1)" "(2)" "(3)" labels appended to core numbers denote different
</pre></div>
2348
bacteria_ice
2009-11-25T11:49:37-05:00
2009-11-25T11:49:37-05:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:2348
Ice core bacteria data from RVIB Nathaniel B. Palmerand ARSV Laurence M. Gould cruises NBP0104, LMG0106, NBP0204,and LMG0205 in the Southern Ocean from 2001-2002 (SOGLOBEC project; Sea Ice Microbes project)
Ice core bacteria data from RVIB Nathaniel B. Palmerand ARSV Laurence M. Gould cruises NBP0104, LMG0106, NBP0204,and LMG0205 in the Southern Ocean from 2001-2002 (SOGLOBEC project; Sea Ice Microbes project)
false
Fritsen, C. (2003) Ice core bacteria data from RVIB Nathaniel B. Palmerand ARSV Laurence M. Gould cruises NBP0104, LMG0106, NBP0204,and LMG0205 in the Southern Ocean from 2001-2002 (SOGLOBEC project; Sea Ice Microbes project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2003-12-03 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.2348.1 [access date]
true
1
10.1575/1912/bco-dmo.2348.1
false
2003-12-03
Datapackage.json
Frictionless Data Package
https://www.bco-dmo.org/dataset/2348/datapackage.json
application/vnd.datapackage+json
PDF
https://www.bco-dmo.org/dataset/2348/Dataset_description.pdf
application/pdf
ISO 19115-2 (NOAA Profile)
https://www.bco-dmo.org/dataset/2348/iso
application/xml
http://www.isotc211.org/2005/gmd-noaa
Dublin Core
https://www.bco-dmo.org/dataset/2348/dublin-core
application/xml
http://purl.org/dc/elements/1.1/
2348
http://lod.bco-dmo.org/id/dataset/2348
2001-07-28 - 2002-09-07
2001-07-28
2001-07-28
---28
208
--07
2001
2002-09-07
2002-09-07
---07
249
--09
2002
OSPREY
http://www.opengis.net/def/crs/OGC/1.3/CRS84
<http://www.opengis.net/def/crs/OGC/1.3/CRS84> POLYGON((-76.781 -69.25, -65.61 -69.25, -65.61 -69.25, -65.61 -65.62, -76.781 -65.62, -76.781 -69.25))
-76.781
-69.25
-65.61
-65.62
-67.435000000000
-71.195500000000
VIEW TABLE
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_2348.htmlTable
text/html
SUBSET DATA
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_2348.html
text/html
CSV
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_2348.csv
text/csv
TSV
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_2348.tsv
text/tsv
GeoJSON
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_2348.geoJson
application/geo+json
MATLAB
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_2348.mat
text/x-matlab
netCDF
https://erddap.bco-dmo.org/erddap/tabledap/bcodmo_dataset_2348.nc
application/x-netcdf