http://lod.bco-dmo.org/id/dataset/2350
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2009-11-25
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Water column bacteria data from ARSV Laurence M. Gould cruises LMG0104 and LMG0106 in the Southern Ocean in 2001 (SOGLOBEC project; Sea Ice Microbes project)
2002-11-15
publication
2002-11-15
revision
National Oceanographic Data Center (NODC)
2013-08-29
publication
http://accession.nodc.noaa.gov/0112521
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2020-01-29
publication
https://doi.org/10.1575/1912/bco-dmo.2350.1
Chris H. Fritsen
Desert Research Institute
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Fritsen, C. (2002) Water column bacteria data from ARSV Laurence M. Gould cruises LMG0104 and LMG0106 in the Southern Ocean in 2001 (SOGLOBEC project; Sea Ice Microbes project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2002-11-15 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.2350.1 [access date]
Water column bacteria data Dataset Description: <h2>Bacteria Abundance, Biomass and Chlorophyll: Water Column Samples<em>a</em></h2>
<p><strong>BG 235 - Methods used for chlorophyll <em>a</em> (chla) analysis and bacteria biomass determination</strong></p>
<p><strong>Core Sampling techniques:</strong></p>
<p>Sampling methods for recovery of chlorophyll <em>a</em> and bacteria from sea ice cores follows those described in&nbsp;<br />
Garrison and Buck (1986)</p>
<p>Recommendations for reporting were used as outlined by:&nbsp;Horner, R. et al.,(1992)</p>
<p><strong>Analytic Techniques:</strong></p>
<p>Chla (mg m<sup>-3</sup>):</p>
<ul>
<li>determined fluorometrically (Turner Designs 10AU Fluorometer) following extraction in 90% acetone (Parsons <em>et al</em>., 1984)</li>
<li>ice core chla corrected to account for chla in filtered sea water (FSW) added to core sections during melting</li>
</ul>
<p>Bacteria cell abundance (cells m<sup>-3</sup>) and biomass (mg C m<sup>-3</sup>):</p>
<p><strong>LMG 0106</strong></p>
<ul>
<li>preserved (0.5% glutaraldehyde) samples stained with 4',6-diamidino-2-phenylindole (DAPI; 0.1 to 0.3% final concentration), filtered through 0.2 mm black, polycarbonate membrane filters, and mounted onto glass microscope slides on the ship (within 24 hours following collection)</li>
<li>bacteria enumerated using epifluorescence microscopy and sized using digital images taken with Image Pro Plus</li>
<li>bacteria biomass determined using cell abundance, cell biovolume (BV; mm<sup>3</sup>; as determined from mean length and width measurements), and an allometric conversion factor for bacterial carbon per volume specific for DAPI-stained bacteria (cellular carbon = 218 X BV<sup>0.86</sup>; Loferer-Krossbacher <em>et al.</em>, 1998).</li>
<li>ice core samples corrected for FSW dilution</li>
</ul>
<p><strong>NBP 0104</strong></p>
<ul>
<li>preserved (0.5% glutaraldehyde) samples stained with SYBR Gold (0.01% final concentration), filtered through 0.2 mm Anodisc filters (Whatman), and mounted onto glass microscope slides at home institution (~1-2 months following collection)</li>
<li>bacteria enumerated using epifluorescence microscopy and sized using digital images taken with Image Pro Plus</li>
<li>bacteria biomass determined using cell abundance, cell biovolume (BV; mm<sup>3</sup>), and an allometric conversion factor for bacterial carbon per volume specific for Acridine Orange-stained bacteria (cellular carbon = 89.9 X BV<sup>0.59</sup>; Simon and Azam, 1989). Note: an AO-specific carbon per volume conversion factor was used in calculating biomass in SYBR Gold-stained samples because both AO and SYBR Gold stain bacteria cells similarly relative to DAPI (unpublished data).</li>
<li>ice core samples corrected for FSW dilution</li>
</ul>
<p>Data from <strong>LMG0106</strong> (July-August, 2001) added in June 2002.</p>
<p><em>Updated: April 21, 2006</em></p> Methods and Sampling: <pre>
# Bacteria data from Southern Ocean GLOBEC
# C. Fritsen and F. Stewart
# * BactAbun = bacterial abundance = cells m-3
# * BactBio = bacterial biomass = mg C m-3 = ug C l-1
# * chla = mg chla m-3 = ug chla l-1
</pre>
Funding provided by NSF Antarctic Sciences (NSF ANT) Award Number: ANT-9910098 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=9910098
completed
Chris H. Fritsen
Desert Research Institute
775/673-7487
Desert Research Inst. Div. of Earth and Ecosystem Sciences, 2215 Raggio Parkway
Reno
NV
89512
USA
Chris.Fritsen@dri.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
cruiseid
year
station
event
month_gmt
day_gmt
lat
lon
depth
bact_abun
bact_biomass_C
chl_a_ugm
Niskin Bottle
theme
None, User defined
cruise id
year
station
event
month_gmt
day_gmt
latitude
longitude
depth
bacterial abundance
bacterial carbon biomass
chl_a_ugm
featureType
BCO-DMO Standard Parameters
Niskin bottle
instrument
BCO-DMO Standard Instruments
LMG0104
LMG0106
service
Deployment Activity
Southern Ocean
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
U.S. GLOBal ocean ECosystems dynamics
http://www.usglobec.org/
U.S. GLOBal ocean ECosystems dynamics
U.S. GLOBEC (GLOBal ocean ECosystems dynamics) is a research program organized by oceanographers and fisheries scientists to address the question of how global climate change may affect the abundance and production of animals in the sea.
The U.S. GLOBEC Program currently had major research efforts underway in the Georges Bank / Northwest Atlantic Region, and the Northeast Pacific (with components in the California Current and in the Coastal Gulf of Alaska). U.S. GLOBEC was a major contributor to International GLOBEC efforts in the Southern Ocean and Western Antarctic Peninsula (WAP).
U.S. GLOBEC
largerWorkCitation
program
U.S. GLOBal ocean ECosystems dynamics
http://www.usglobec.org/
U.S. GLOBal ocean ECosystems dynamics
U.S. GLOBEC (GLOBal ocean ECosystems dynamics) is a research program organized by oceanographers and fisheries scientists to address the question of how global climate change may affect the abundance and production of animals in the sea.
The U.S. GLOBEC Program currently had major research efforts underway in the Georges Bank / Northwest Atlantic Region, and the Northeast Pacific (with components in the California Current and in the Coastal Gulf of Alaska). U.S. GLOBEC was a major contributor to International GLOBEC efforts in the Southern Ocean and Western Antarctic Peninsula (WAP).
U.S. GLOBEC
largerWorkCitation
program
U.S. GLOBEC Southern Ocean
http://www.ccpo.odu.edu/Research/globec_menu.html
U.S. GLOBEC Southern Ocean
<p>The fundamental objectives of United States Global Ocean Ecosystems Dynamics (U.S. GLOBEC) Program are dependent upon the cooperation of scientists from several disciplines. Physicists, biologists, and chemists must make use of data collected during U.S. GLOBEC field programs to further our understanding of the interplay of physics, biology, and chemistry. Our objectives require quantitative analysis of interdisciplinary data sets and, therefore, data must be exchanged between researchers. To extract the full scientific value, data must be made available to the scientific community on a timely basis.</p>
SOGLOBEC
largerWorkCitation
project
GLOBEC: Sea Ice Microbial Communities
https://osprey.bco-dmo.org/project/616163
GLOBEC: Sea Ice Microbial Communities
<p>The U.S. Global Ocean Ecosystems Dynamics (U.S. GLOBEC) program has the goal of understanding and ultimately predicting how populations of marine animal species respond to natural and anthropogenic changes in climate. Research in the Southern Ocean (SO) indicates strong coupling between climatic processes and ecosystem dynamics via the annual formation and destruction of sea ice. The Southern Ocean GLOBEC Program (SO GLOBEC) will investigate the dynamic relationship between physical processes and ecosystem responses through identification of critical parameters that affect the distribution, abundance and population dynamics of target species. The overall goals of the SO GLOBEC program are to elucidate shelf circulation processes and their effect on sea ice formation and krill distribution, and to examine the factors which govern krill survivorship and availability to higher trophic levels, including penguins, seals and whales. The focus of the U.S. contribution to the international SO GLOBEC program will be on winter processes. This component will focus on the distribution and activities of sea ice microbial communities. This will be accomplished using an integrated combination of sampling (vertical profiles, horizontal surveys, and under-ice surveys) and observational protocols. Experiments will be designed to estimate microbial activity within the sea ice and at the ice-seawater interface. The research will be coordinated with components studying the water column productivity and the sea ice habitat. The result of the integrated SO GLOBEC program will be to improve the predictability of living marine resources, especially with respect to local and global climatic shifts.</p>
Sea Ice Microbes
largerWorkCitation
project
eng; USA
biota
oceans
Southern Ocean
-72.427
-67.049
-69.888
-65.965
2001-04-29
2001-08-20
Southern Ocean
0
BCO-DMO catalogue of parameters from Water column bacteria data from ARSV Laurence M. Gould cruises LMG0104 and LMG0106 in the Southern Ocean in 2001 (SOGLOBEC project; Sea Ice Microbes project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/7488.rdf
Name: cruiseid
Units: unknown
Description: <p>cruise identification</p>
http://lod.bco-dmo.org/id/dataset-parameter/7489.rdf
Name: year
Units: unknown
Description: <p>year</p>
http://lod.bco-dmo.org/id/dataset-parameter/7490.rdf
Name: station
Units: dimensionless
Description: <p>station identification</p>
http://lod.bco-dmo.org/id/dataset-parameter/7491.rdf
Name: event
Units: dimensionless
Description: <p>event number from event log</p>
http://lod.bco-dmo.org/id/dataset-parameter/7492.rdf
Name: month_gmt
Units: GMT
Description: <p>month of year</p>
http://lod.bco-dmo.org/id/dataset-parameter/7493.rdf
Name: day_gmt
Units: GMT
Description: <p>day of month</p>
http://lod.bco-dmo.org/id/dataset-parameter/7494.rdf
Name: lat
Units: decimal degrees
Description: <p>latitude, negative = South</p>
http://lod.bco-dmo.org/id/dataset-parameter/7495.rdf
Name: lon
Units: decimal degrees
Description: <p>longitude, negative = West</p>
http://lod.bco-dmo.org/id/dataset-parameter/7496.rdf
Name: depth
Units: meters
Description: <p>depth of sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/7497.rdf
Name: bact_abun
Units: cells/meter<sup>3</sup>
Description: <p>bacteria abundance</p>
http://lod.bco-dmo.org/id/dataset-parameter/7498.rdf
Name: bact_biomass_C
Units: milligrams C/meter<sup>3</sup>
Description: <p>bacteria carbon biomass</p>
http://lod.bco-dmo.org/id/dataset-parameter/7499.rdf
Name: chl_a_ugm
Units: micrograms/liter
Description: <p>chlorophyll <i>a</i> concentration</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
17749
https://darchive.mblwhoilibrary.org/bitstream/1912/25284/1/dataset-2350_bacteriawater__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.2350.1
download
onLine
dataset
<pre>
# Bacteria data from Southern Ocean GLOBEC
# C. Fritsen and F. Stewart
# * BactAbun = bacterial abundance = cells m-3
# * BactBio = bacterial biomass = mg C m-3 = ug C l-1
# * chla = mg chla m-3 = ug chla l-1
</pre>
from Cruise: LMG0104 <pre>
# Bacteria data from Southern Ocean GLOBEC
# C. Fritsen and F. Stewart
# * BactAbun = bacterial abundance = cells m-3
# * BactBio = bacterial biomass = mg C m-3 = ug C l-1
# * chla = mg chla m-3 = ug chla l-1
</pre>
from Cruise: LMG0106 <pre>
# Bacteria data from Southern Ocean GLOBEC
# C. Fritsen and F. Stewart
# * BactAbun = bacterial abundance = cells m-3
# * BactBio = bacterial biomass = mg C m-3 = ug C l-1
# * chla = mg chla m-3 = ug chla l-1
</pre>
Specified by the Principal Investigator(s)
<p>Chla (mg m<sup>-3</sup>):</p>
<ul>
<li>determined fluorometrically (Turner Designs 10AU Fluorometer) following extraction in 90% acetone (Parsons <em>et al</em>., 1984)</li>
<li>ice core chla corrected to account for chla in filtered sea water (FSW) added to core sections during melting</li>
</ul>
<p>Bacteria cell abundance (cells m<sup>-3</sup>) and biomass (mg C m<sup>-3</sup>):</p>
<p><strong>LMG 0106</strong></p>
<ul>
<li>preserved (0.5% glutaraldehyde) samples stained with 4',6-diamidino-2-phenylindole (DAPI; 0.1 to 0.3% final concentration), filtered through 0.2 mm black, polycarbonate membrane filters, and mounted onto glass microscope slides on the ship (within 24 hours following collection)</li>
<li>bacteria enumerated using epifluorescence microscopy and sized using digital images taken with Image Pro Plus</li>
<li>bacteria biomass determined using cell abundance, cell biovolume (BV; mm<sup>3</sup>; as determined from mean length and width measurements), and an allometric conversion factor for bacterial carbon per volume specific for DAPI-stained bacteria (cellular carbon = 218 X BV<sup>0.86</sup>; Loferer-Krossbacher et al., 1998).</li>
<li>ice core samples corrected for FSW dilution</li>
</ul>
from Cruise: LMG0104
<b>Chla (mg m-3):</b>
<ul>
<li> determined fluorometrically (Turner Designs 10AU Fluorometer) following extraction in 90% acetone (Parsons et al., 1984)
<li> ice core chla corrected to account for chla in filtered sea water (FSW) added to core sections during melting
</ul>
from Cruise: LMG0106 Chla (mg m<sup>-3</sup>):
</p><ul>
<li>determined fluorometrically (Turner Designs 10AU
Fluorometer) following extraction in 90% acetone (Parsons <i>et al</i>., 1984)</li>
<li>ice core chla
corrected to account for chla in filtered sea water (FSW) added to core
sections during melting</li>
</ul>
<p>Bacteria cell abundance (cells m<sup>-3</sup>) and
biomass (mg C m<sup>-3</sup>):
</p><p>LMG 0106</p>
<ul>
<li>preserved (0.5%
glutaraldehyde) samples stained with 4',6-diamidino-2-phenylindole (DAPI; 0.1
to 0.3% final concentration), filtered through 0.2 mm black, polycarbonate membrane filters, and
mounted onto glass microscope slides on the ship (within 24 hours following
collection)</li>
<li>bacteria enumerated
using epifluorescence microscopy and sized using digital images taken with
Image Pro Plus</li>
<li>bacteria biomass determined
using cell abundance, cell biovolume (BV; mm<sup>3</sup>;
as determined from mean length and width measurements), and an allometric
conversion factor for bacterial carbon per volume specific for DAPI-stained
bacteria
(cellular carbon = 218 X BV<sup>0.86</sup>; Loferer-Krößbacher <i>et al.</i>, 1998).</li>
<li>ice core samples
corrected for FSW dilution</li>
</ul>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Niskin Bottle
Niskin Bottle
PI Supplied Instrument Name: Niskin Bottle PI Supplied Instrument Description:Niskin bottle cast, use Bottle_Niskin Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Cruise: LMG0104
LMG0104
ARSV Laurence M. Gould
Community Standard Description
International Council for the Exploration of the Sea
ARSV Laurence M. Gould
vessel
LMG0104
Dr Joseph J. Torres
University of South Florida
http://www.ccpo.odu.edu/Research/globec/cruises/gould0103_0104.doc
Report describing LMG0104
Cruise: LMG0106
LMG0106
ARSV Laurence M. Gould
Community Standard Description
International Council for the Exploration of the Sea
ARSV Laurence M. Gould
vessel
LMG0106
Daniel P. Costa
University of California-San Diego
http://www.ccpo.odu.edu/Research/globec/cruises01/lmg0106_menu.html
Report describing LMG0106
ARSV Laurence M. Gould
Community Standard Description
International Council for the Exploration of the Sea
ARSV Laurence M. Gould
vessel