http://lod.bco-dmo.org/id/dataset/2475
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2009-11-29
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Phytoplankton growth rates and microzooplankton grazing from R/V Alpha Helix HX242, HX244, HX247, HX271, HX275 in the Northeast Pacific from 2001-2003
2007-03-28
publication
2007-03-28
revision
National Oceanographic Data Center (NODC)
2013-11-06
publication
http://accession.nodc.noaa.gov/0114241
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-02-18
publication
https://doi.org/10.1575/1912/bco-dmo.2475.1
Suzanne Strom
Western Washington University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Strom, S. (2007) Phytoplankton growth rates and microzooplankton grazing from R/V Alpha Helix HX242, HX244, HX247, HX271, HX275 in the Northeast Pacific from 2001-2003. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2007-03-28 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.2475.1 [access date]
Phytoplankton growth rates and microzoo grazing Dataset Description: <p><strong>Phytoplankton Growth Rates in the coastal Gulf of Alaska</strong></p>
<p>Phytoplankton growth rates, the phytoplankton growth rate response to nutrient enrichment, and microzooplankton grazing rates on phytoplankton in three different size classes were measured during three cruises to the coastal Gulf of Alaska in 2001.</p> Methods and Sampling: <p>[excerpted from Strom, et al. (2006) Microzooplankton grazing in the coastal Gulf of Alaska: Variations in top-down control of phytoplankton. Limnol Oceanogr in press.]</p>
<p>Water drawn from multiple Niskin bottles closed at a single depth was pooled into two 25-liter polycarbonate carboys. Most often, water was collected from the depth corresponding to 50% of surface irradiance (50% Io, 3 to 10 m). Once during April, and once per station during July, water was collected from the depth of the subsurface chlorophyll maximum (SCM, 12 to 25 m). The contents of one carboy were gravity-filtered (0.2 um) to generate particle-free filtered seawater (FSW, the diluent for the dilution series). The contents of the other were gently pre-screened through 200 um Nitex mesh to exclude macrozooplankton (WSW, the whole seawater for the dilution series). Using gentle siphoning and mixing techniques, FSW and WSW were combined in known proportions in 2.35-liter polycarbonate bottles to generate a dilution series consisting of 9, 16, 24, 41, 61, and 100% WSW (each in duplicate). An additional pair of bottles diluted to 4% was added during the May and July cruises, as well as an additional pair of 100% WSW bottles to control for the effects of nutrient enrichment on phytoplankton growth rate. Clean techniques and inert materials (silicone, polycarbonate) were used throughout.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0101397 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0101397
Funding provided by National Oceanic and Atmospheric Administration (NOAA) Award Number: unknown NEP NOAA
completed
Suzanne Strom
Western Washington University
360-293-2188
Shannon Point Marine Center 1900 Shannon Point Rd
Anacortes
WA
98221
USA
suzanne.strom@wwu.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
cruiseid
expt
date_local
station_std
lat
lon
time_start_local
depth
chla_start_gt20um
chla_start_5_to_20um
chla_start_lt5um
chla_start_total
fed_phyto_growth_gt20um
fed_phyto_growth_5_to_20um
fed_phyto_growth_lt5um
fed_phyto_growth_total
fed_phyto_growth_std_err_gt20um
fed_phyto_growth_std_err_5_to_20um
fed_phyto_growth_std_err_lt5um
fed_phyto_growth_std_err_total
unfed_phyto_growth_gt20um
unfed_phyto_growth_5_to_20um
unfed_phyto_growth_lt5um
unfed_phyto_growth_total
unfed_phyto_growth_std_err_gt20um
unfed_phyto_growth_std_err_5_to_20um
unfed_phyto_growth_std_err_lt5um
unfed_phyto_growth_std_err_total
microzoo_graz_gt20um
microzoo_graz_5_to_20um
microzoo_graz_lt5um
microzoo_graz_total
microzoo_graz_std_err_gt20um
microzoo_graz_std_err_5_to_20um
microzoo_graz_std_err_lt5um
microzoo_graz_std_err_total
temp
sal
irradiance_mol_per_m2
Niskin Bottle
theme
None, User defined
cruise id
No BCO-DMO term
date_local
station_std
latitude
longitude
time_start_local
depth
water temperature at measurement depth
salinity
irradiance
featureType
BCO-DMO Standard Parameters
Niskin bottle
instrument
BCO-DMO Standard Instruments
HX242
HX244
HX247
HX271
HX275
service
Deployment Activity
Northeast Pacific
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
U.S. GLOBal ocean ECosystems dynamics
http://www.usglobec.org/
U.S. GLOBal ocean ECosystems dynamics
U.S. GLOBEC (GLOBal ocean ECosystems dynamics) is a research program organized by oceanographers and fisheries scientists to address the question of how global climate change may affect the abundance and production of animals in the sea.
The U.S. GLOBEC Program currently had major research efforts underway in the Georges Bank / Northwest Atlantic Region, and the Northeast Pacific (with components in the California Current and in the Coastal Gulf of Alaska). U.S. GLOBEC was a major contributor to International GLOBEC efforts in the Southern Ocean and Western Antarctic Peninsula (WAP).
U.S. GLOBEC
largerWorkCitation
program
U.S. GLOBEC Northeast Pacific
http://nepglobec.bco-dmo.org
U.S. GLOBEC Northeast Pacific
<p><strong>Program in a Nutshell</strong></p>
<p><strong>Goal: </strong> To understand the effects of climate variability and climate change on the distribution, abundance and production of marine animals (including commercially important living marine resources) in the eastern North Pacific. To embody this understanding in diagnostic and prognostic ecosystem models, capable of capturing the ecosystem response to major climatic fluctuations.</p>
<p><strong>Approach: </strong>To study the effects of past and present climate variability on the population ecology and population dynamics of marine biota and living marine resources, and to use this information as a proxy for how the ecosystems of the eastern North Pacific may respond to future global climate change. The strong temporal variability in the physical and biological signals of the NEP will be used to examine the biophysical mechanisms through which zooplankton and salmon populations respond to physical forcing and biological interactions in the coastal regions of the two gyres. Annual and interannual variability will be studied directly through <strong>long-term observations</strong> and detailed <strong>process studies</strong>; variability at longer time scales will be examined through <strong>retrospective analysis</strong> of directly measured and proxy data. Coupled <strong>biophysical models</strong> of the ecosystems of these regions will be developed and tested using the process studies and data collected from the long-term observation programs, then further tested and improved by hindcasting selected retrospective data series.</p>
NEP
largerWorkCitation
project
eng; USA
biota
oceans
Northeast Pacific
-150.419
-146.607
58.097
60.535
2001-04-17
2003-08-09
Northeast Pacific Ocean, Gulf of Alaska
0
BCO-DMO catalogue of parameters from Phytoplankton growth rates and microzooplankton grazing from R/V Alpha Helix HX242, HX244, HX247, HX271, HX275 in the Northeast Pacific from 2001-2003
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/9018.rdf
Name: cruiseid
Units: text
Description: <p>Cruise ID.</p>
http://lod.bco-dmo.org/id/dataset-parameter/9019.rdf
Name: expt
Units: dimensionless
Description: <p>Experiment number.</p>
http://lod.bco-dmo.org/id/dataset-parameter/9020.rdf
Name: date_local
Units: dd-mon-yy
Description: <p>Day-month-year.</p>
http://lod.bco-dmo.org/id/dataset-parameter/9021.rdf
Name: station_std
Units: dimensionless
Description: <p>standard station number</p>
http://lod.bco-dmo.org/id/dataset-parameter/9022.rdf
Name: lat
Units: decimal degrees (North is positive)
Description: <p> Latitude </p>
http://lod.bco-dmo.org/id/dataset-parameter/9023.rdf
Name: lon
Units: decimal degrees (East is positive)
Description: <p> Longitude </p>
http://lod.bco-dmo.org/id/dataset-parameter/9024.rdf
Name: time_start_local
Units: 24-hour clock
Description: <p> experiment start time</p>
http://lod.bco-dmo.org/id/dataset-parameter/9025.rdf
Name: depth
Units: meters
Description: <p>water collection depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/9026.rdf
Name: chla_start_gt20um
Units: micrograms/liter
Description: <p>initial chlorophyll concentration <br />for dilutions experiments</p>
http://lod.bco-dmo.org/id/dataset-parameter/9027.rdf
Name: chla_start_5_to_20um
Units: micrograms/liter
Description: <p>initial chlorophyll concentration <br />for dilutions experiments</p>
http://lod.bco-dmo.org/id/dataset-parameter/9028.rdf
Name: chla_start_lt5um
Units: micrograms/liter
Description: <p>initial chlorophyll concentration <br />for dilutions experiments</p>
http://lod.bco-dmo.org/id/dataset-parameter/9029.rdf
Name: chla_start_total
Units: micrograms/liter
Description: <p>sum of three size fractions</p>
http://lod.bco-dmo.org/id/dataset-parameter/9030.rdf
Name: fed_phyto_growth_gt20um
Units: per day
Description: <p>enriched (addition of nitrate and phosphate) <br />phytoplankton growth rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/9031.rdf
Name: fed_phyto_growth_5_to_20um
Units: per day
Description: <p>enriched (addition of nitrate and phosphate) <br />phytoplankton growth rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/9032.rdf
Name: fed_phyto_growth_lt5um
Units: per day
Description: <p>enriched (addition of nitrate and phosphate) <br />phytoplankton growth rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/9033.rdf
Name: fed_phyto_growth_total
Units: per day
Description: <p>sum of three size fractions</p>
http://lod.bco-dmo.org/id/dataset-parameter/9034.rdf
Name: fed_phyto_growth_std_err_gt20um
Units: per day
Description: <p>enriched phytoplankton growth rate standard error</p>
http://lod.bco-dmo.org/id/dataset-parameter/9035.rdf
Name: fed_phyto_growth_std_err_5_to_20um
Units: per day
Description: <p>enriched phytoplankton growth rate standard error</p>
http://lod.bco-dmo.org/id/dataset-parameter/9036.rdf
Name: fed_phyto_growth_std_err_lt5um
Units: per day
Description: <p>enriched phytoplankton growth rate standard error</p>
http://lod.bco-dmo.org/id/dataset-parameter/9037.rdf
Name: fed_phyto_growth_std_err_total
Units: per day
Description: <p>total standard error</p>
http://lod.bco-dmo.org/id/dataset-parameter/9038.rdf
Name: unfed_phyto_growth_gt20um
Units: per day
Description: <p>unenriched phytoplankton growth rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/9039.rdf
Name: unfed_phyto_growth_5_to_20um
Units: per day
Description: <p>unenriched phytoplankton growth rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/9040.rdf
Name: unfed_phyto_growth_lt5um
Units: per day
Description: <p>unenriched phytoplankton growth rate</p>
http://lod.bco-dmo.org/id/dataset-parameter/9041.rdf
Name: unfed_phyto_growth_total
Units: per day
Description: <p>sum of three size fractions</p>
http://lod.bco-dmo.org/id/dataset-parameter/9042.rdf
Name: unfed_phyto_growth_std_err_gt20um
Units: per day
Description: <p>unenriched phytoplankton growth rate standard error</p>
http://lod.bco-dmo.org/id/dataset-parameter/9043.rdf
Name: unfed_phyto_growth_std_err_5_to_20um
Units: per day
Description: <p>unenriched phytoplankton growth rate standard error</p>
http://lod.bco-dmo.org/id/dataset-parameter/9044.rdf
Name: unfed_phyto_growth_std_err_lt5um
Units: per day
Description: <p>unenriched phytoplankton growth rate standard error</p>
http://lod.bco-dmo.org/id/dataset-parameter/9045.rdf
Name: unfed_phyto_growth_std_err_total
Units: per day
Description: <p>total standard error</p>
http://lod.bco-dmo.org/id/dataset-parameter/9046.rdf
Name: microzoo_graz_gt20um
Units: per day
Description: <p>microzooplankton grazing rate<br /> for >20µ size fraction</p>
http://lod.bco-dmo.org/id/dataset-parameter/9047.rdf
Name: microzoo_graz_5_to_20um
Units: per day
Description: <p>microzooplankton grazing rate<br /> for >5 and <20µ size fraction</p>
http://lod.bco-dmo.org/id/dataset-parameter/9048.rdf
Name: microzoo_graz_lt5um
Units: per day
Description: <p>microzooplankton grazing rate<br /> for <5µ size fraction</p>
http://lod.bco-dmo.org/id/dataset-parameter/9049.rdf
Name: microzoo_graz_total
Units: per day
Description: <p>total microzooplankton grazing rate for all size fractions</p>
http://lod.bco-dmo.org/id/dataset-parameter/9050.rdf
Name: microzoo_graz_std_err_gt20um
Units: per day
Description: <p>standard error</p>
http://lod.bco-dmo.org/id/dataset-parameter/9051.rdf
Name: microzoo_graz_std_err_5_to_20um
Units: per day
Description: <p>standard error</p>
http://lod.bco-dmo.org/id/dataset-parameter/9052.rdf
Name: microzoo_graz_std_err_lt5um
Units: per day
Description: <p>standard error</p>
http://lod.bco-dmo.org/id/dataset-parameter/9053.rdf
Name: microzoo_graz_std_err_total
Units: per day
Description: <p>total standard error</p>
http://lod.bco-dmo.org/id/dataset-parameter/755939.rdf
Name: temp
Units: degrees Celsius
Description: <p>temperature at sample depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/755940.rdf
Name: sal
Units: Practical Salinity Units (PSU)
Description: <p>dalinity at sample depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/755941.rdf
Name: irradiance_mol_per_m2
Units: mol photons/meter^2(per second?)
Description: <p>irradiance at sample depth</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
24687
https://darchive.mblwhoilibrary.org/bitstream/1912/23652/1/dataset-2475_phytogrowth__v1.tsv
download
https://doi.org/10.1575/1912/bco-dmo.2475.1
download
onLine
dataset
<p>[excerpted from Strom, et al. (2006) Microzooplankton grazing in the coastal Gulf of Alaska: Variations in top-down control of phytoplankton. Limnol Oceanogr in press.]</p>
<p>Water drawn from multiple Niskin bottles closed at a single depth was pooled into two 25-liter polycarbonate carboys. Most often, water was collected from the depth corresponding to 50% of surface irradiance (50% Io, 3 to 10 m). Once during April, and once per station during July, water was collected from the depth of the subsurface chlorophyll maximum (SCM, 12 to 25 m). The contents of one carboy were gravity-filtered (0.2 um) to generate particle-free filtered seawater (FSW, the diluent for the dilution series). The contents of the other were gently pre-screened through 200 um Nitex mesh to exclude macrozooplankton (WSW, the whole seawater for the dilution series). Using gentle siphoning and mixing techniques, FSW and WSW were combined in known proportions in 2.35-liter polycarbonate bottles to generate a dilution series consisting of 9, 16, 24, 41, 61, and 100% WSW (each in duplicate). An additional pair of bottles diluted to 4% was added during the May and July cruises, as well as an additional pair of 100% WSW bottles to control for the effects of nutrient enrichment on phytoplankton growth rate. Clean techniques and inert materials (silicone, polycarbonate) were used throughout.</p>
from Cruise: HX242 [excerpted from Strom, et al. (2006) Microzooplankton grazing in the coastal Gulf of Alaska: Variations in top-down control of phytoplankton. Limnol Oceanogr in press.]
<p>
Water drawn from multiple Niskin bottles closed at a single depth was pooled into two 25-liter polycarbonate carboys. Most often, water was collected from the depth corresponding to 50% of surface irradiance (50% Io, 3 to 10 m). Once during April, and once per station during July, water was collected from the depth of the subsurface chlorophyll maximum (SCM, 12 to 25 m). The contents of one carboy were gravity-filtered (0.2 µm) to generate particle-free filtered seawater (FSW, the diluent for the dilution series). The contents of the other were gently pre-screened through 200 µm Nitex mesh to exclude macrozooplankton (WSW, the whole seawater for the dilution series). Using gentle siphoning and mixing techniques, FSW and WSW were combined in known proportions in 2.35-liter polycarbonate bottles to generate a dilution series consisting of 9, 16, 24, 41, 61, and 100% WSW (each in duplicate). An additional pair of bottles diluted to 4% was added during the May and July cruises, as well as an additional pair of 100% WSW bottles to control for the effects of nutrient enrichment on phytoplankton growth rate. Clean techniques and inert materials (silicone, polycarbonate) were used throughout.
from Cruise: HX244 [excerpted from Strom, et al. (2006) Microzooplankton grazing in the coastal Gulf of Alaska: Variations in top-down control of phytoplankton. Limnol Oceanogr in press.]
<p>
Water drawn from multiple Niskin bottles closed at a single depth was pooled into two 25-liter polycarbonate carboys. Most often, water was collected from the depth corresponding to 50% of surface irradiance (50% Io, 3 to 10 m). Once during April, and once per station during July, water was collected from the depth of the subsurface chlorophyll maximum (SCM, 12 to 25 m). The contents of one carboy were gravity-filtered (0.2 µm) to generate particle-free filtered seawater (FSW, the diluent for the dilution series). The contents of the other were gently pre-screened through 200 µm Nitex mesh to exclude macrozooplankton (WSW, the whole seawater for the dilution series). Using gentle siphoning and mixing techniques, FSW and WSW were combined in known proportions in 2.35-liter polycarbonate bottles to generate a dilution series consisting of 9, 16, 24, 41, 61, and 100% WSW (each in duplicate). An additional pair of bottles diluted to 4% was added during the May and July cruises, as well as an additional pair of 100% WSW bottles to control for the effects of nutrient enrichment on phytoplankton growth rate. Clean techniques and inert materials (silicone, polycarbonate) were used throughout.
from Cruise: HX247 [excerpted from Strom, et al. (2006) Microzooplankton grazing in the coastal Gulf of Alaska: Variations in top-down control of phytoplankton. Limnol Oceanogr in press.]
<p>
Water drawn from multiple Niskin bottles closed at a single depth was pooled into two 25-liter polycarbonate carboys. Most often, water was collected from the depth corresponding to 50% of surface irradiance (50% Io, 3 to 10 m). Once during April, and once per station during July, water was collected from the depth of the subsurface chlorophyll maximum (SCM, 12 to 25 m). The contents of one carboy were gravity-filtered (0.2 µm) to generate particle-free filtered seawater (FSW, the diluent for the dilution series). The contents of the other were gently pre-screened through 200 µm Nitex mesh to exclude macrozooplankton (WSW, the whole seawater for the dilution series). Using gentle siphoning and mixing techniques, FSW and WSW were combined in known proportions in 2.35-liter polycarbonate bottles to generate a dilution series consisting of 9, 16, 24, 41, 61, and 100% WSW (each in duplicate). An additional pair of bottles diluted to 4% was added during the May and July cruises, as well as an additional pair of 100% WSW bottles to control for the effects of nutrient enrichment on phytoplankton growth rate. Clean techniques and inert materials (silicone, polycarbonate) were used throughout.
Specified by the Principal Investigator(s)
<p>Initial samples for size-fractionated chlorophyll (&lt;5, 5 to 20, and &gt;20 um, in quadruplicate), nutrients (nitrate, nitrite, silicic acid, phosphate), and microzooplankton abundance and composition (in duplicate, see below) were taken from the WSW carboy at intervals during experiment set-up. Initial chlorophyll levels in diluted bottles were calculated from these measured WSW values and known dilution factors. Coefficients of variation for quadruplicate initial chlorophyll samples averaged 7.9%, 13.5%, and 8.9% for the &lt;5, 5 to 20, and &gt;20 um size fractions, respectively. During May (all but outer shelf experiments) and July cruises, all diluted bottles and two 100% WSW bottles were enriched with nitrate (4.7 umol L-1 as NaNO3) and phosphate (0.27 umol L-1 as Na2HPO4). The other two 100% WSW bottles were left unenriched. Bottles were screened to collection-depth light levels with neutral density screening and incubated on deck in seawater-cooled incubators for 24 hr. All bottles were then sampled in duplicate for size-fractionated chlorophyll (filtration volumes ranged from 0.15 to 1.08 liter depending on WSW chlorophyll and dilution levels); 100% WSW bottles were additionally sampled for microzooplankton abundance and composition.</p>
<p>Net growth rates (k, d-1) for total chlorophyll and individual chlorophyll size fractions were calculated as (1/t)(ln[Pt/Po]), where Pt = final chlorophyll concentration, Po = initial chlorophyll concentration, and t = incubation time in d. Intrinsic growth rates (µ,d-1) of phytoplankton were estimated from the y-intercept of net growth rates regressed upon fraction WSW. For experiments exhibiting saturated grazing (i.e. a leveling of net growth rate across the least-dilute bottles) (Gallegos 1989), instrinsic growth rate estimates were based on regression of net growth rates in only the most dilute bottles (generally those with &lt;40% WSW). Microzooplankton grazing rates (g, d-1) were estimated from the slope of the regression for experiments with linear relationships between net growth and fraction WSW, and as g = µ<sub>n</sub> - k<sub>n</sub> (where k<sub>n</sub> = net growth rate of phytoplankton in enriched, 100% WSW bottles) for experiments with saturated grazing. In experiments with nutrient enrichment, unenriched phytoplankton growth rates (µ<sub>o</sub>) were calculated as µ<sub>o</sub> = k<sub>o</sub> + g, where k<sub>o</sub> = net growth rate of phytoplankton in unenriched, 100% WSW bottles. Estimates of µ<sub>o</sub> were used to compare microzooplankton grazing to phytoplankton growth in situ (g : µ<sub>o</sub>). These ratios represent the fraction of primary production consumed each day by microzooplankton grazing. Ratios were arctan transformed for estimation of means and standard deviations.</p>
<p>More detailed methods reference in&nbsp;Strom, <em>et al.</em> (2006).</p>
from Cruise: HX242 <p style="text-indent: 0.25in;"> Initial samples for size-fractionated chlorophyll (<5, 5 to 20, and >20 µm, in
quadruplicate), nutrients (nitrate, nitrite, silicic acid, phosphate), and microzooplankton
abundance and composition (in duplicate, see below) were taken from the WSW carboy
at intervals during experiment set-up. Initial chlorophyll levels in diluted bottles were
calculated from these measured WSW values and known dilution factors. Coefficients of
variation for quadruplicate initial chlorophyll samples averaged 7.9%, 13.5%, and 8.9%
for the <5, 5 to 20, and >20 µm size fractions, respectively. During May (all but outer
shelf experiments) and July cruises, all diluted bottles and two 100% WSW bottles were
enriched with nitrate (4.7 µmol L-1 as NaNO3) and phosphate (0.27 µmol L-1 as
Na2HPO4). The other two 100% WSW bottles were left unenriched. Bottles were
screened to collection-depth light levels with neutral density screening and incubated on
deck in seawater-cooled incubators for 24 hr. All bottles were then sampled in duplicate
for size-fractionated chlorophyll (filtration volumes ranged from 0.15 to 1.08 liter
depending on WSW chlorophyll and dilution levels); 100% WSW bottles were
additionally sampled for microzooplankton abundance and composition. </p>
<p style="text-indent: 0.25in;"> Net growth rates (k, d-1) for total chlorophyll and individual chlorophyll size
fractions were calculated as (1/t)(ln[Pt/Po]), where Pt = final chlorophyll concentration,
Po = initial chlorophyll concentration, and t = incubation time in d. Intrinsic growth rates
(µ,d-1) of phytoplankton were estimated from the y-intercept of net growth rates
regressed upon fraction WSW. For experiments exhibiting saturated grazing (i.e. a
leveling of net growth rate across the least-dilute bottles) (Gallegos 1989), instrinsic
growth rate estimates were based on regression of net growth rates in only the most dilute
bottles (generally those with ?40% WSW). Microzooplankton grazing rates
(g, d-1) were
estimated from the slope of the regression for experiments with linear
relationships
between net growth and fraction WSW, and as g = µ<sub>n</sub> - k<sub>n</sub>
(where k<sub>n</sub> = net growth rate of
phytoplankton in enriched, 100% WSW bottles) for experiments with saturated
grazing.
In experiments with nutrient enrichment, unenriched phytoplankton growth rates
(µ<sub>o</sub>)
were calculated as µ<sub>o</sub> = k<sub>o</sub> + g, where k<sub>o</sub> = net
growth rate of phytoplankton in
unenriched, 100% WSW bottles. Estimates of µ<sub>o</sub> were used to compare
microzooplankton grazing to phytoplankton growth in situ (g : µ<sub>o</sub>).
These ratios
represent the fraction of primary production consumed each day by
microzooplankton
grazing. Ratios were arctan transformed for estimation of means and standard
deviations. </p>
<p>More detailed methods reference:<br>
Strom, <i>et al.</i> (2006) Cross-shelf gradients in phytoplankton community structure, nutrient
utilization, and growth rate in the coastal Gulf of Alaska. <i>Marine Ecology
Progress Series</i> (in press)</p>
from Cruise: HX244 <p style="text-indent: 0.25in;"> Initial samples for size-fractionated chlorophyll (<5, 5 to 20, and >20 µm, in
quadruplicate), nutrients (nitrate, nitrite, silicic acid, phosphate), and microzooplankton
abundance and composition (in duplicate, see below) were taken from the WSW carboy
at intervals during experiment set-up. Initial chlorophyll levels in diluted bottles were
calculated from these measured WSW values and known dilution factors. Coefficients of
variation for quadruplicate initial chlorophyll samples averaged 7.9%, 13.5%, and 8.9%
for the <5, 5 to 20, and >20 µm size fractions, respectively. During May (all but outer
shelf experiments) and July cruises, all diluted bottles and two 100% WSW bottles were
enriched with nitrate (4.7 µmol L-1 as NaNO3) and phosphate (0.27 µmol L-1 as
Na2HPO4). The other two 100% WSW bottles were left unenriched. Bottles were
screened to collection-depth light levels with neutral density screening and incubated on
deck in seawater-cooled incubators for 24 hr. All bottles were then sampled in duplicate
for size-fractionated chlorophyll (filtration volumes ranged from 0.15 to 1.08 liter
depending on WSW chlorophyll and dilution levels); 100% WSW bottles were
additionally sampled for microzooplankton abundance and composition. </p>
<p style="text-indent: 0.25in;"> Net growth rates (k, d-1) for total chlorophyll and individual chlorophyll size
fractions were calculated as (1/t)(ln[Pt/Po]), where Pt = final chlorophyll concentration,
Po = initial chlorophyll concentration, and t = incubation time in d. Intrinsic growth rates
(µ,d-1) of phytoplankton were estimated from the y-intercept of net growth rates
regressed upon fraction WSW. For experiments exhibiting saturated grazing (i.e. a
leveling of net growth rate across the least-dilute bottles) (Gallegos 1989), instrinsic
growth rate estimates were based on regression of net growth rates in only the most dilute
bottles (generally those with ?40% WSW). Microzooplankton grazing rates
(g, d-1) were
estimated from the slope of the regression for experiments with linear
relationships
between net growth and fraction WSW, and as g = µ<sub>n</sub> - k<sub>n</sub>
(where k<sub>n</sub> = net growth rate of
phytoplankton in enriched, 100% WSW bottles) for experiments with saturated
grazing.
In experiments with nutrient enrichment, unenriched phytoplankton growth rates
(µ<sub>o</sub>)
were calculated as µ<sub>o</sub> = k<sub>o</sub> + g, where k<sub>o</sub> = net
growth rate of phytoplankton in
unenriched, 100% WSW bottles. Estimates of µ<sub>o</sub> were used to compare
microzooplankton grazing to phytoplankton growth in situ (g : µ<sub>o</sub>).
These ratios
represent the fraction of primary production consumed each day by
microzooplankton
grazing. Ratios were arctan transformed for estimation of means and standard
deviations. </p>
<p>More detailed methods reference:<br>
Strom, <i>et al.</i> (2006) Cross-shelf gradients in phytoplankton community structure, nutrient
utilization, and growth rate in the coastal Gulf of Alaska. <i>Marine Ecology
Progress Series</i> (in press)</p>
from Cruise: HX247 <p style="text-indent: 0.25in;"> Initial samples for size-fractionated chlorophyll (<5, 5 to 20, and >20 µm, in
quadruplicate), nutrients (nitrate, nitrite, silicic acid, phosphate), and microzooplankton
abundance and composition (in duplicate, see below) were taken from the WSW carboy
at intervals during experiment set-up. Initial chlorophyll levels in diluted bottles were
calculated from these measured WSW values and known dilution factors. Coefficients of
variation for quadruplicate initial chlorophyll samples averaged 7.9%, 13.5%, and 8.9%
for the <5, 5 to 20, and >20 µm size fractions, respectively. During May (all but outer
shelf experiments) and July cruises, all diluted bottles and two 100% WSW bottles were
enriched with nitrate (4.7 µmol L-1 as NaNO3) and phosphate (0.27 µmol L-1 as
Na2HPO4). The other two 100% WSW bottles were left unenriched. Bottles were
screened to collection-depth light levels with neutral density screening and incubated on
deck in seawater-cooled incubators for 24 hr. All bottles were then sampled in duplicate
for size-fractionated chlorophyll (filtration volumes ranged from 0.15 to 1.08 liter
depending on WSW chlorophyll and dilution levels); 100% WSW bottles were
additionally sampled for microzooplankton abundance and composition. </p>
<p style="text-indent: 0.25in;"> Net growth rates (k, d-1) for total chlorophyll and individual chlorophyll size
fractions were calculated as (1/t)(ln[Pt/Po]), where Pt = final chlorophyll concentration,
Po = initial chlorophyll concentration, and t = incubation time in d. Intrinsic growth rates
(µ,d-1) of phytoplankton were estimated from the y-intercept of net growth rates
regressed upon fraction WSW. For experiments exhibiting saturated grazing (i.e. a
leveling of net growth rate across the least-dilute bottles) (Gallegos 1989), instrinsic
growth rate estimates were based on regression of net growth rates in only the most dilute
bottles (generally those with ?40% WSW). Microzooplankton grazing rates
(g, d-1) were
estimated from the slope of the regression for experiments with linear
relationships
between net growth and fraction WSW, and as g = µ<sub>n</sub> - k<sub>n</sub>
(where k<sub>n</sub> = net growth rate of
phytoplankton in enriched, 100% WSW bottles) for experiments with saturated
grazing.
In experiments with nutrient enrichment, unenriched phytoplankton growth rates
(µ<sub>o</sub>)
were calculated as µ<sub>o</sub> = k<sub>o</sub> + g, where k<sub>o</sub> = net
growth rate of phytoplankton in
unenriched, 100% WSW bottles. Estimates of µ<sub>o</sub> were used to compare
microzooplankton grazing to phytoplankton growth in situ (g : µ<sub>o</sub>).
These ratios
represent the fraction of primary production consumed each day by
microzooplankton
grazing. Ratios were arctan transformed for estimation of means and standard
deviations. </p>
<p>More detailed methods reference:<br>
Strom, <i>et al.</i> (2006) Cross-shelf gradients in phytoplankton community structure, nutrient
utilization, and growth rate in the coastal Gulf of Alaska. <i>Marine Ecology
Progress Series</i> (in press)</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Niskin Bottle
Niskin Bottle
PI Supplied Instrument Name: Niskin Bottle PI Supplied Instrument Description:Niskin bottle cast, use Bottle_Niskin Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Cruise: HX242
HX242
R/V Alpha Helix
Community Standard Description
International Council for the Exploration of the Sea
R/V Alpha Helix
vessel
HX242
Suzanne Strom
Western Washington University
http://globec.whoi.edu/nep/reports/cgoa_cruises/hx242cr.pdf
Report describing HX242
Cruise: HX244
HX244
R/V Alpha Helix
Community Standard Description
International Council for the Exploration of the Sea
R/V Alpha Helix
vessel
HX244
Suzanne Strom
Western Washington University
http://globec.whoi.edu/nep/reports/cgoa_cruises/hx244cr.pdf
Report describing HX244
Cruise: HX247
HX247
R/V Alpha Helix
Community Standard Description
International Council for the Exploration of the Sea
R/V Alpha Helix
vessel
HX247
Dr Jeffrey Napp
National Oceanic and Atmospheric Administration - Alaska Fisheries Science Center
http://globec.whoi.edu/nep/reports/cgoa_cruises/hx247cr.pdf
Report describing HX247
Cruise: HX271
HX271
R/V Alpha Helix
Community Standard Description
International Council for the Exploration of the Sea
R/V Alpha Helix
vessel
HX271
Suzanne Strom
Western Washington University
http://globec.whoi.edu/nep/reports/cgoa_cruises/hx271cr.pdf
Report describing HX271
Cruise: HX275
HX275
R/V Alpha Helix
Community Standard Description
International Council for the Exploration of the Sea
R/V Alpha Helix
vessel
HX275
Dr Jeffrey Napp
National Oceanic and Atmospheric Administration - Alaska Fisheries Science Center
http://globec.whoi.edu/nep/reports/cgoa_cruises/hx275cr.pdf
Report describing HX275
R/V Alpha Helix
Community Standard Description
International Council for the Exploration of the Sea
R/V Alpha Helix
vessel