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                <gco:CharacterString>&amp;lt;p&amp;gt;See Platform deployments for cruise specific documentation&amp;lt;/p&amp;gt;

from Cruise: TT050  
 &lt;pre&gt;
   &lt;b&gt;PI:&lt;/b&gt;              David Garrison and Marcia Gowing
   &lt;b&gt;of:&lt;/b&gt;              University of California-Santa Cruz
   &lt;b&gt;dataset:&lt;/b&gt;         Microzooplankton abundance and carbon biomass
   &lt;b&gt;dates:&lt;/b&gt;           August 19, 1995 to September 12, 1995
   &lt;b&gt;location:&lt;/b&gt;        N: 22.4308  S: 9.9769  W: 57.3004  E: 68.7385
   &lt;b&gt;project/cruise:&lt;/b&gt;  Arabian Sea/TTN-050 - Process Cruise 5 (Late SW Monsoon)
   &lt;b&gt;ship:&lt;/b&gt;            Thomas Thompson
 

   Note:  Abundance of nanoflagellates represent the 2-20 micron size class.
   All other abundances represent the 20-200 micron range.
 


DMO note:
Marcia Gowing and David Garrison
Microzooplankton abundances &amp; biomass
Thomas Thompson cruise TTN-050, Process Cruise 5, Arabian Sea

The following samples were collected by pooling multiple bottles of the same cast (usually 60 liters).
In the main data set, only a single bottle number is listed.

event___  sta_std  sta  cast  bot_single  bot_multi
08290158  S15      13   7     24          19-24
08290158  S15      13   7     18          13-18
08290158  S15      13   7     12           7-12
08290158  S15      13   7      6           1-6
08290252  S15      13   8      6           1-6
09010054  S11      17   3     24          19-24
09010054  S11      17   3     18          13-18
09010054  S11      17   3     12           7-12
09010054  S11      17   3      6           1-6
09010157  S11      17   4     15          10-15
09050059  S7       21   7     24          19-24
09050059  S7       21   7     18          13-18
09050059  S7       21   7     12           7-12
09050059  S7       21   7      6           1-6
09050156  S7       21   8      6           1-6
09080057  S4       24   6     24          19-24
09080057  S4       24   6     18          13-18
09080057  S4       24   6     12           7-12
09080057  S4       24   6      6           1-6
09080148  S4       24   7      6           1-6
09110048  S2       26   7     24          19-24
09110048  S2       26   7     18          13-18
09110048  S2       26   7     12           7-12
09110048  S2       26   7      6           1-6
09110138  S2       26   8      6           1-6
09121126  S1       27   1     22          21-22
09121126  S1       27   1     18          17-18
09121126  S1       27   1     14          13-14
09121126  S1       27   1     10           9-10
09121126  S1       27   1      6           5-6
&lt;/pre&gt;


from Cruise: TT054  
 &lt;pre&gt;
   &lt;b&gt;PI:&lt;/b&gt;              David Garrison and Marcia Gowing
   &lt;b&gt;of:&lt;/b&gt;              University of California - Santa Cruz
   &lt;b&gt;dataset:&lt;/b&gt;         Microzooplankton abundance and carbon biomass
   &lt;b&gt;dates:&lt;/b&gt;           December 05, 1995 to December 24, 1995
   &lt;b&gt;location:&lt;/b&gt;        N: 19.2079  S: 9.9674  W: 58.1373  E: 67.1739
   &lt;b&gt;project/cruise:&lt;/b&gt;  Arabian Sea/TTN-054 - Process Cruise 7 (Early NE Monsoon)
   &lt;b&gt;ship:&lt;/b&gt;            Thomas Thompson
 
   
   Note:  Abundance of nanoflagellates represent the 2-20 micron size class.
   All other abundances represent the 20-200 micron range.
 
   &lt;a href=&quot;http://usjgofs.whoi.edu/jg/serv/jgofs/arabian/ttn-054/microzoo_note.html0{dir=usjgofs.whoi.edu/jg/dir/jgofs/arabian/ttn-054/}&quot;&gt;DMO Note on multiple-bottle events&lt;/a&gt; 
&lt;/pre&gt;</gco:CharacterString>
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                <gco:CharacterString>&amp;lt;pre&amp;gt;
David Garrison and Marcia Gowing

Microzooplankton Methods

Samples for microzooplankton biomass were obtained by taking ~ 2-liter
aliquots from standard hydrographic casts. Heterotrophic flagellates
and some nano-planktonic non-loricate ciliates were examined by
epifluorescence microscopy on samples preserved with approximately 0.5%
glutaraldehyde, concentrated on 0.8 micron, black Nuclepore filters,
and stained with the fluorochromes DAPI (Coleman 1980) and proflavin
(Haas 1982) following the protocol outlined by Verity and Sieracki
(1993).  Whole water samples were preserved with buffered
paraformaldehyde.  Larger heterotrophic dinoflagellates, and most of
the ciliates were enumerated from 50 or 100 ml of these preserved
samples that were settled and counted with an inverted microscope.
Biomass was estimated by converting cell volumes (calculated from
measurements of cell dimensions) using the relationship ((Log10 C =
0.94 (log10 V)-0.60); with C representing carbon as picograms per cell
and V representing total cell volume in cubic microns) for flagellates
(Eppley et al. 1970), and the relationship carbon per cell = 0.16
pgC/cubic micron (Stoecker et al. 1994).

Coleman, A.W.  1980.  Enhanced staining of bacteria in natural
environments by fluorochrome staining of DNA.  Limnol. Oceanogr.
25:948-951.

Eppley, R.W., F.M.H. Reid, J.D.H. Strickland.  1970.  The ecology of
the plankton off La Jolla, California, in the period April through
September, 1967.  (ed. J.D.H. Strickland), pt. III, Estimates of
phytoplankton crop size, growth rate and primary production.  Bull.
Scripps Inst. Oceanogr.  17:33-42.

Haas, L.W.  1982.  Improved epifluorescence microscopy for observing
planktonic microorganisms.  Ann. Inst. Oceanogr. Paris.  58S:261-266.

Stoecker, D.K., D.J. Gifford, and M. Putt.  1994.  Preservation of
marine planktonic ciliates: losses and cell shrinkage during fixation.
Mar. Ecol. Prog. Ser.  110:293-299.

Verity, P.G. and M.E.Sieracki.  1993.  Use of color image analysis and
epifluorescence microscopy to measure plankton biomass. In: (Kemp,
P.F., B.F. Sherr,E.B. Sherr, and J.C. Cole, eds.)  Aquatic Microbial
Ecology.  pp. 327-338.  Lewis Publishers, Boca Raton.
&amp;lt;/pre&amp;gt;

from Cruise: TT050 &lt;pre&gt;
David Garrison and Marcia Gowing

Microzooplankton Methods

Samples for microzooplankton biomass were obtained by taking ~ 2-liter
aliquots from standard hydrographic casts. Heterotrophic flagellates
and some nano-planktonic non-loricate ciliates were examined by
epifluorescence microscopy on samples preserved with approximately 0.5%
glutaraldehyde, concentrated on 0.8 micron, black Nuclepore filters,
and stained with the fluorochromes DAPI (Coleman 1980) and proflavin
(Haas 1982) following the protocol outlined by Verity and Sieracki
(1993).  Whole water samples were preserved with buffered
paraformaldehyde.  Larger heterotrophic dinoflagellates, and most of
the ciliates were enumerated from 50 or 100 ml of these preserved
samples that were settled and counted with an inverted microscope.
Biomass was estimated by converting cell volumes (calculated from
measurements of cell dimensions) using the relationship ((Log10 C =
0.94 (log10 V)-0.60); with C representing carbon as picograms per cell
and V representing total cell volume in cubic microns) for flagellates
(Eppley et al. 1970), and the relationship carbon per cell = 0.16
pgC/cubic micron (Stoecker et al. 1994).

Coleman, A.W.  1980.  Enhanced staining of bacteria in natural
environments by fluorochrome staining of DNA.  Limnol. Oceanogr.
25:948-951.

Eppley, R.W., F.M.H. Reid, J.D.H. Strickland.  1970.  The ecology of
the plankton off La Jolla, California, in the period April through
September, 1967.  (ed. J.D.H. Strickland), pt. III, Estimates of
phytoplankton crop size, growth rate and primary production.  Bull.
Scripps Inst. Oceanogr.  17:33-42.

Haas, L.W.  1982.  Improved epifluorescence microscopy for observing
planktonic microorganisms.  Ann. Inst. Oceanogr. Paris.  58S:261-266.

Stoecker, D.K., D.J. Gifford, and M. Putt.  1994.  Preservation of
marine planktonic ciliates: losses and cell shrinkage during fixation.
Mar. Ecol. Prog. Ser.  110:293-299.

Verity, P.G. and M.E.Sieracki.  1993.  Use of color image analysis and
epifluorescence microscopy to measure plankton biomass. In: (Kemp,
P.F., B.F. Sherr,E.B. Sherr, and J.C. Cole, eds.)  Aquatic Microbial
Ecology.  pp. 327-338.  Lewis Publishers, Boca Raton.
&lt;/pre&gt;





from Cruise: TT054 &lt;pre&gt;
David Garrison and Marcia Gowing

Microzooplankton Methods

Samples for microzooplankton biomass were obtained by taking ~ 2-liter
aliquots from standard hydrographic casts. Heterotrophic flagellates
and some nano-planktonic non-loricate ciliates were examined by
epifluorescence microscopy on samples preserved with approximately 0.5%
glutaraldehyde, concentrated on 0.8 micron, black Nuclepore filters,
and stained with the fluorochromes DAPI (Coleman 1980) and proflavin
(Haas 1982) following the protocol outlined by Verity and Sieracki
(1993).  Whole water samples were preserved with buffered
paraformaldehyde.  Larger heterotrophic dinoflagellates, and most of
the ciliates were enumerated from 50 or 100 ml of these preserved
samples that were settled and counted with an inverted microscope.
Biomass was estimated by converting cell volumes (calculated from
measurements of cell dimensions) using the relationship ((Log10 C =
0.94 (log10 V)-0.60); with C representing carbon as picograms per cell
and V representing total cell volume in cubic microns) for flagellates
(Eppley et al. 1970), and the relationship carbon per cell = 0.16
pgC/cubic micron (Stoecker et al. 1994).

Coleman, A.W.  1980.  Enhanced staining of bacteria in natural
environments by fluorochrome staining of DNA.  Limnol. Oceanogr.
25:948-951.

Eppley, R.W., F.M.H. Reid, J.D.H. Strickland.  1970.  The ecology of
the plankton off La Jolla, California, in the period April through
September, 1967.  (ed. J.D.H. Strickland), pt. III, Estimates of
phytoplankton crop size, growth rate and primary production.  Bull.
Scripps Inst. Oceanogr.  17:33-42.

Haas, L.W.  1982.  Improved epifluorescence microscopy for observing
planktonic microorganisms.  Ann. Inst. Oceanogr. Paris.  58S:261-266.

Stoecker, D.K., D.J. Gifford, and M. Putt.  1994.  Preservation of
marine planktonic ciliates: losses and cell shrinkage during fixation.
Mar. Ecol. Prog. Ser.  110:293-299.

Verity, P.G. and M.E.Sieracki.  1993.  Use of color image analysis and
epifluorescence microscopy to measure plankton biomass. In: (Kemp,
P.F., B.F. Sherr,E.B. Sherr, and J.C. Cole, eds.)  Aquatic Microbial
Ecology.  pp. 327-338.  Lewis Publishers, Boca Raton.
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