http://lod.bco-dmo.org/id/dataset/2620
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2010-06-16
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Amino acid flux data collected from the U.S. JGOFS Eqpac Moored Sediment Trap Array in the Equatorial Pacific in 1992 during the U.S. JGOFS Equatorial Pacific (EqPac) project
2002-01-02
publication
2002-01-02
revision
BCO-DMO Linked Data URI
2002-01-02
creation
http://lod.bco-dmo.org/id/dataset/2620
Cindy Lee
Stony Brook University
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Lee, C. (2002) Amino acid flux data collected from the U.S. JGOFS Eqpac Moored Sediment Trap Array in the Equatorial Pacific in 1992 during the U.S. JGOFS Equatorial Pacific (EqPac) project. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version January 2, 2002) Version Date 2002-01-02 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/2620 [access date]
Amino acid fluxes from moored sediment traps Dataset Description: <p>Amino acid fluxes from moored sediment traps</p> Methods and Sampling: <pre>
<b>PI:</b> Cindy Lee
<b>of:</b> State University New York, Stony Brook
<b>dataset:</b> Amino acid fluxes from moored sediment traps
<b>dates:</b> February 3, 1992 to December 13, 1992
<b>location:</b> N: 9 S: 0 W: -140 E: -140
<b>project/cruise:</b> EqPac/W9201B Sediment trap mooring deployment cruise
<b>ship:</b> R/V Wecoma
</pre>
<p>&nbsp;</p>
<h2>US JGOFS EqPac: Chloropigments and Amino Acid Concentrations in Sediment Cores and Traps</h2>
<pre><b> Cindy Lee EqPac (cruise 13 for collection of sediment cores) Chloropigments and Amino Acid Concentrations in Sediment Cores and Traps 2.2. Analytical methods </b>
For more detail on this data set, see: Lee, C; Wakeham,S.G.; Hedges, J.I., 2000.
Composition and flux of particulate amino acids and chloropigments in equatorial Pacific
seawater and sediments. Deep-Sea Research, I, 47 ( 8), 1535-1568.
Deep sediment traps were also deployed between January 1992, and January 1993, on
moorings (Honjo et al., 1995) at 9&deg;N, 5&deg;N and 0&deg;. Both IRS-valved traps and valveless
(NVC no-valve control) traps collected samples from about 1000 m below the sea
surface, while NVC traps collected samples from ~1000 m above the sea floor (mab).
Traps were poisoned with mercuric chloride, and retention of poison was verified by
salinity measurements. Particulate material collected was split and filtered as for free-
drifting traps
Sediment cores were collected during November-December, 1992, from seven stations
along the N-S transect using a multiple corer (Barnett et al., 1984). These stations were
at 9, 5, 2&deg;N, the equator, and 2, 5 and 12&deg;S. Cores were sectioned aboard ship; similar
sediment depth intervals from several cores taken simultaneously were composited and
homogenized.
Particulate amino acids were measured by fluorescence high performance liquid
chromatography (HPLC) after acid hydrolysis (Lee and Cronin, 1982; 1984). Thawed
filters and sediments were hydrolyzed under N2 at 110degC for 19 h with 6 N HCl to
release THAA, total hydrolyzable amino acids in peptide bonds (proteins and peptides) or
adsorbed onto particles. Hydrolyzates were dried in vacuo, taken up in water, and the
resulting free amino acids were analyzed by HPLC using a modification of the Mopper
and Lindroth (1982) o-phthaldialdehyde derivative technique. Only one sample was
usually available from each site and depth for hydrolysis because of the splitting scheme.
Duplicate analyses of the same hydrolyzate agreed within 10-15% except for lysine
(~40%).
We report here results only for chlorophyll-a and some of its immediate degradation
products. Chloropigments were extracted from thawed filters into 100% acetone and
analyzed by HPLC with fluorescence detection (Mantoura and Llewellyn, 1983; Bidigare
et al., 1985). Samples were covered with Al foil as much as possible during handling and
analysis to exclude light. Details of our analytical methods appear in Sun and Sun. Here
we report data on chlorophyll-a (Chl), pheophytin-a (Phytin), pheophorbide-a (Phide) and
pyropheophorbide-a (Pyrophide) fluxes and composition. Monovinyl and divinyl
chlorophylls (Bidigare and Ondrusek, 1997) were not separated. Only one sample was
usually available from each site and depth for extraction because of the splitting scheme.
Duplicate analyses of the same extract agreed within 10%.
<hr />
Barnett, R.P.O., Watson, J., Connelly, D. 1984. A multiple corer for taking virtually
undisturbed samples form shelf, bathyal and abyssal sediments. Oceanologica Acta 7,
399-408.
Bidigare, R.R., Kennicutt, M.C., Brookes, J.M. 1985. Rapid determination of
chlorophylls and their degradation products by high performance chromatography.
Limnology and Oceanography 30, 432-435.
Bidigare, R.R., Ondrusek, M.E. 1996. Spatial and temporal variability of phytoplankton
pigment distributions in the central equatorial Pacific Ocean. Deep-Sea Research II 43,
809-833.
Honjo S., Dymond, J., Collier, R. and Manganini, S.J., 1995. Export production of
particles to the interior of the equatorial Pacific Ocean during the 1992 EqPac
experiment. Deep-Sea Research II 42, pp. 831&iuml;&iquest;&frac12;870.
Lee, C., Cronin, C. 1982. The vertical flux of particulate organic nitrogen in the sea:
decomposition of amino acids in the Peru upwelling area and the equatorial Atlantic,
Journal of Marine Research 40, 227-251.
Lee, C., Cronin, C. 1984. Particulate amino acids in the sea: Effects of primary
productivity and biological decomposition. Journal of Marine Research 42, 1075-1097.
Mantoura,R.F.C., Llewellyn, C.A. 1983. The rapid determination of algal chlorophyll and
carotenoid pigments and their breakdown products in natural waters by reverse-phase
high-performance liquid chromatography. Analytica Chimica Acta 151, 297-314.
Mopper, K., Lindroth, P. 1982. Diel and depth variations in dissolved free amino acids
and ammonium in the Baltic Sea determined by shipboard HPLC analyses. Limnology
and Oceanography 27, 336-347
</pre>
completed
Cindy Lee
Stony Brook University
631-632-8741
109 Challenger Hall
Stony Brook
NY
11794-5000
USA
Cindy.Lee@stonybrook.edu
pointOfContact
asNeeded
Dataset Version: January 2, 2002
Unknown
mooring
lat_n
lon_n
depth_trap
trap_type
Asp_f
Glu_f
Ser_f
His_f
Gly_f
Thr_f
Arg_f
Ala_f
Tyr_f
Met_f
Val_f
p_Ala_f
Ile_f
Leu_f
Lys_f
amino_flux
High Performance Liquid Chromatography
IRS Sediment Trap
theme
None, User defined
mooring identification
No BCO-DMO term
featureType
BCO-DMO Standard Parameters
High-Performance Liquid Chromatograph
Sediment Trap - IRS
instrument
BCO-DMO Standard Instruments
EqPac-Array
service
Deployment Activity
U.S. JGOFS Equatorial Pacific
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
U.S. Joint Global Ocean Flux Study
http://usjgofs.whoi.edu/
U.S. Joint Global Ocean Flux Study
The United States Joint Global Ocean Flux Study was a national component of international JGOFS and an integral part of global climate change research.
The U.S. launched the Joint Global Ocean Flux Study (JGOFS) in the late 1980s to study the ocean carbon cycle. An ambitious goal was set to understand the controls on the concentrations and fluxes of carbon and associated nutrients in the ocean. A new field of ocean biogeochemistry emerged with an emphasis on quality measurements of carbon system parameters and interdisciplinary field studies of the biological, chemical and physical process which control the ocean carbon cycle. As we studied ocean biogeochemistry, we learned that our simple views of carbon uptake and transport were severely limited, and a new "wave" of ocean science was born. U.S. JGOFS has been supported primarily by the U.S. National Science Foundation in collaboration with the National Oceanic and Atmospheric Administration, the National Aeronautics and Space Administration, the Department of Energy and the Office of Naval Research. U.S. JGOFS, ended in 2005 with the conclusion of the Synthesis and Modeling Project (SMP).
U.S. JGOFS
largerWorkCitation
program
U.S. JGOFS Equatorial Pacific
http://usjgofs.whoi.edu/research/eqpac.html
U.S. JGOFS Equatorial Pacific
<p>The U.S. EqPac process study consisted of repeat meridional sections (12°N -12°S) across the equator in the central and eastern equatorial Pacific from 95°W to 170°W during 1992. The major scientific program was focused at 140° W consisting of two meridional surveys, two equatorial surveys, and a benthic survey aboard the R/V Thomas Thompson. Long-term deployments of current meter and sediment trap arrays augmented the survey cruises. NOAA conducted boreal spring and fall sections east and west of 140°W from the R/V Baldridge and R/V Discoverer. Meteorological and sea surface observations were obtained from NOAA's in place TOGA-TAO buoy network.</p>
<p>The scientific objectives of this study were to determine the fluxes of carbon and related elements, and the processes controlling these fluxes between the Equatorial Pacific euphotic zone and the atmosphere and deep ocean. A broad overview of the program at the 140°W site is given by Murray et al. (Oceanography, 5: 134-142, 1992). A full description of the Equatorial Pacific Process Study, including the international context and the scientific results, appears in a series of Deep-Sea Research Part II special volumes:</p>
<p>Topical Studies in Oceanography, A U.S. JGOFS Process Study in the Equatorial Pacific (1995), Deep-Sea Research Part II, Volume 42, No. 2/3.</p>
<p>Topical Studies in Oceanography, A U.S. JGOFS Process Study in the Equatorial Pacific. Part 2 (1996), Deep-Sea Research Part II, Volume 43, No. 4/6.</p>
<p>Topical Studies in Oceanography, A U.S. JGOFS Process Study in the Equatorial Pacific (1997), Deep-Sea Research Part II, Volume 44, No. 9/10.</p>
<p>Topical Studies in Oceanography, The Equatorial Pacific JGOFS Synthesis (2002), Deep-Sea Research Part II, Volume 49, Nos. 13/14.</p>
EqPac
largerWorkCitation
project
eng; USA
oceans
U.S. JGOFS Equatorial Pacific
2002-01-02
Equatorial Pacific
0
BCO-DMO catalogue of parameters from Amino acid flux data collected from the U.S. JGOFS Eqpac Moored Sediment Trap Array in the Equatorial Pacific in 1992 during the U.S. JGOFS Equatorial Pacific (EqPac) project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/11085.rdf
Name: mooring
Units: dimensionless
Description: <p>mooring identification</p>
http://lod.bco-dmo.org/id/dataset-parameter/11086.rdf
Name: lat_n
Units: degrees
Description: <p>nominal latitude, minus = south</p>
http://lod.bco-dmo.org/id/dataset-parameter/11087.rdf
Name: lon_n
Units: degrees
Description: <p>nominal longitude, minus = west</p>
http://lod.bco-dmo.org/id/dataset-parameter/11088.rdf
Name: depth_trap
Units: meters
Description: <p>depth of trap</p>
http://lod.bco-dmo.org/id/dataset-parameter/11089.rdf
Name: trap_type
Units: unknown
Description: <p>type of trap where:<br />
IRS = Indented Rotary Sphere NVC = Non-Valve Control</p>
http://lod.bco-dmo.org/id/dataset-parameter/11090.rdf
Name: Asp_f
Units: milligrams/m2/day
Description: <p>aspartic acid flux</p>
http://lod.bco-dmo.org/id/dataset-parameter/11091.rdf
Name: Glu_f
Units: milligrams/m2/day
Description: <p>glutamic acid flux</p>
http://lod.bco-dmo.org/id/dataset-parameter/11092.rdf
Name: Ser_f
Units: milligrams/m2/day
Description: <p>serine flux</p>
http://lod.bco-dmo.org/id/dataset-parameter/11093.rdf
Name: His_f
Units: milligrams/m2/day
Description: <p>histidine flux</p>
http://lod.bco-dmo.org/id/dataset-parameter/11094.rdf
Name: Gly_f
Units: milligrams/m2/day
Description: <p>glycine flux</p>
http://lod.bco-dmo.org/id/dataset-parameter/11095.rdf
Name: Thr_f
Units: milligrams/m2/day
Description: <p>threonine flux</p>
http://lod.bco-dmo.org/id/dataset-parameter/11096.rdf
Name: Arg_f
Units: milligrams/m2/day
Description: <p>arginine flux</p>
http://lod.bco-dmo.org/id/dataset-parameter/11097.rdf
Name: Ala_f
Units: milligrams/m2/day
Description: <p>alanine flux</p>
http://lod.bco-dmo.org/id/dataset-parameter/11098.rdf
Name: Tyr_f
Units: milligrams/m2/day
Description: <p>tyrosine flux</p>
http://lod.bco-dmo.org/id/dataset-parameter/11099.rdf
Name: Met_f
Units: milligrams/m2/day
Description: <p>methionine flux</p>
http://lod.bco-dmo.org/id/dataset-parameter/11100.rdf
Name: Val_f
Units: milligrams/m2/day
Description: <p>valine flux</p>
http://lod.bco-dmo.org/id/dataset-parameter/11101.rdf
Name: p_Ala_f
Units: milligrams/m2/day
Description: <p>phenylalanine flux</p>
http://lod.bco-dmo.org/id/dataset-parameter/11102.rdf
Name: Ile_f
Units: milligrams/m2/day
Description: <p>isoleucine flux</p>
http://lod.bco-dmo.org/id/dataset-parameter/11103.rdf
Name: Leu_f
Units: milligrams/m2/day
Description: <p>leucine flux</p>
http://lod.bco-dmo.org/id/dataset-parameter/11104.rdf
Name: Lys_f
Units: milligrams/m2/day
Description: <p>lysine flux</p>
http://lod.bco-dmo.org/id/dataset-parameter/11105.rdf
Name: amino_flux
Units: milligrams/m2/day
Description: <p>sum, amino acid fluxes</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
1143
https://datadocs.bco-dmo.org/file/JEE1nwPfqmjEyg/amino_acids.csv
amino_acids.csv
Primary data file for dataset ID 2620
download
https://osprey.bco-dmo.org/dataset/2620/data/download
download
onLine
dataset
<pre>
<b>PI:</b> Cindy Lee
<b>of:</b> State University New York, Stony Brook
<b>dataset:</b> Amino acid fluxes from moored sediment traps
<b>dates:</b> February 3, 1992 to December 13, 1992
<b>location:</b> N: 9 S: 0 W: -140 E: -140
<b>project/cruise:</b> EqPac/W9201B Sediment trap mooring deployment cruise
<b>ship:</b> R/V Wecoma
</pre>
<p>&nbsp;</p>
<h2>US JGOFS EqPac: Chloropigments and Amino Acid Concentrations in Sediment Cores and Traps</h2>
<pre><b> Cindy Lee EqPac (cruise 13 for collection of sediment cores) Chloropigments and Amino Acid Concentrations in Sediment Cores and Traps 2.2. Analytical methods </b>
For more detail on this data set, see: Lee, C; Wakeham,S.G.; Hedges, J.I., 2000.
Composition and flux of particulate amino acids and chloropigments in equatorial Pacific
seawater and sediments. Deep-Sea Research, I, 47 ( 8), 1535-1568.
Deep sediment traps were also deployed between January 1992, and January 1993, on
moorings (Honjo et al., 1995) at 9&deg;N, 5&deg;N and 0&deg;. Both IRS-valved traps and valveless
(NVC no-valve control) traps collected samples from about 1000 m below the sea
surface, while NVC traps collected samples from ~1000 m above the sea floor (mab).
Traps were poisoned with mercuric chloride, and retention of poison was verified by
salinity measurements. Particulate material collected was split and filtered as for free-
drifting traps
Sediment cores were collected during November-December, 1992, from seven stations
along the N-S transect using a multiple corer (Barnett et al., 1984). These stations were
at 9, 5, 2&deg;N, the equator, and 2, 5 and 12&deg;S. Cores were sectioned aboard ship; similar
sediment depth intervals from several cores taken simultaneously were composited and
homogenized.
Particulate amino acids were measured by fluorescence high performance liquid
chromatography (HPLC) after acid hydrolysis (Lee and Cronin, 1982; 1984). Thawed
filters and sediments were hydrolyzed under N2 at 110degC for 19 h with 6 N HCl to
release THAA, total hydrolyzable amino acids in peptide bonds (proteins and peptides) or
adsorbed onto particles. Hydrolyzates were dried in vacuo, taken up in water, and the
resulting free amino acids were analyzed by HPLC using a modification of the Mopper
and Lindroth (1982) o-phthaldialdehyde derivative technique. Only one sample was
usually available from each site and depth for hydrolysis because of the splitting scheme.
Duplicate analyses of the same hydrolyzate agreed within 10-15% except for lysine
(~40%).
We report here results only for chlorophyll-a and some of its immediate degradation
products. Chloropigments were extracted from thawed filters into 100% acetone and
analyzed by HPLC with fluorescence detection (Mantoura and Llewellyn, 1983; Bidigare
et al., 1985). Samples were covered with Al foil as much as possible during handling and
analysis to exclude light. Details of our analytical methods appear in Sun and Sun. Here
we report data on chlorophyll-a (Chl), pheophytin-a (Phytin), pheophorbide-a (Phide) and
pyropheophorbide-a (Pyrophide) fluxes and composition. Monovinyl and divinyl
chlorophylls (Bidigare and Ondrusek, 1997) were not separated. Only one sample was
usually available from each site and depth for extraction because of the splitting scheme.
Duplicate analyses of the same extract agreed within 10%.
<hr />
Barnett, R.P.O., Watson, J., Connelly, D. 1984. A multiple corer for taking virtually
undisturbed samples form shelf, bathyal and abyssal sediments. Oceanologica Acta 7,
399-408.
Bidigare, R.R., Kennicutt, M.C., Brookes, J.M. 1985. Rapid determination of
chlorophylls and their degradation products by high performance chromatography.
Limnology and Oceanography 30, 432-435.
Bidigare, R.R., Ondrusek, M.E. 1996. Spatial and temporal variability of phytoplankton
pigment distributions in the central equatorial Pacific Ocean. Deep-Sea Research II 43,
809-833.
Honjo S., Dymond, J., Collier, R. and Manganini, S.J., 1995. Export production of
particles to the interior of the equatorial Pacific Ocean during the 1992 EqPac
experiment. Deep-Sea Research II 42, pp. 831&iuml;&iquest;&frac12;870.
Lee, C., Cronin, C. 1982. The vertical flux of particulate organic nitrogen in the sea:
decomposition of amino acids in the Peru upwelling area and the equatorial Atlantic,
Journal of Marine Research 40, 227-251.
Lee, C., Cronin, C. 1984. Particulate amino acids in the sea: Effects of primary
productivity and biological decomposition. Journal of Marine Research 42, 1075-1097.
Mantoura,R.F.C., Llewellyn, C.A. 1983. The rapid determination of algal chlorophyll and
carotenoid pigments and their breakdown products in natural waters by reverse-phase
high-performance liquid chromatography. Analytica Chimica Acta 151, 297-314.
Mopper, K., Lindroth, P. 1982. Diel and depth variations in dissolved free amino acids
and ammonium in the Baltic Sea determined by shipboard HPLC analyses. Limnology
and Oceanography 27, 336-347
</pre>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
High Performance Liquid Chromatography
High Performance Liquid Chromatography
PI Supplied Instrument Name: High Performance Liquid Chromatography PI Supplied Instrument Description:Fluorescence high performance liquid chromatography (HPLC) was used to measure particulate amino acids Instrument Name: High-Performance Liquid Chromatograph Instrument Short Name:HPLC Instrument Description: A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB11/
IRS Sediment Trap
IRS Sediment Trap
PI Supplied Instrument Name: IRS Sediment Trap Instrument Name: Sediment Trap - IRS Instrument Short Name:Sed Trap - IRS Instrument Description: Sediment traps are specially designed containers deployed in the water column for periods of time to collect particles from the water column falling toward the sea floor. In general a sediment trap has a jar at the bottom to collect the sample and a broad funnel-shaped opening at the top with baffles to keep out very large objects and help prevent the funnel from clogging. The Indented Rotating Sphere (IRS) Sediment Trap is described in Peterson et al. (Field evaluation of a valved sediment trap. 1993. Limnology and Oceanography, 38, pp. 1741-1761 and Novel techniques for collection of sinking particles in the ocean and determining their settling rates. 2005. Limnology and Oceanography Methods 3, pp. 520-532). The IRS trap consists of four cylindrical modules; a particle interceptor, an IRS valve; a skewed funnel, and an eleven sample carousel (designated IRSC trap). The key to the trap design is the patented IRS valve located between the particle interceptor and particle accumulator portions of the trap. The valve and carousel are regulated by a TattleTale IVA (manufactured by Onset Computer Corp.) microprocessor and custom software. The IRS sediment trap was specifically designed to exclude zooplankton (Trull et al. 2008. Deep-Sea Research II v.55 pp. 1684-1695). Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/33/
Deployment: EqPac-Array
EqPac-Array
JGOFS Sediment Trap
mooring
EqPac-Array
Susumu Honjo
Woods Hole Oceanographic Institution
JGOFS Sediment Trap
mooring