http://lod.bco-dmo.org/id/dataset/2845
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2010-06-16
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Bacteria, Microzooplankton/Phytoplankton enumeration, production, grazing and growth from R/V Tangaroa cruise 61TG_3052 in the Southern Ocean in 1999 (SOIREE project)
2010-02-04
publication
2010-02-04
revision
BCO-DMO Linked Data URI
2010-02-04
creation
http://lod.bco-dmo.org/id/dataset/2845
Julie Hall
New Zealand National Institute of Water and Atmospheric Research
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Hall, J. (2010) Bacteria, Microzooplankton/Phytoplankton enumeration, production, grazing and growth from R/V Tangaroa cruise 61TG_3052 in the Southern Ocean in 1999 (SOIREE project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 04Feb2010) Version Date 2010-02-04 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/2845 [access date]
Bacteria Microzoop/Phytoplankton Enumeration, Production, Grazing and Growth Dataset Description: <p>SOIREE Bacterial Microzoop/Phytoplankton Enumeration, Production, Gazing and Growth<br />
&nbsp;</p> Methods and Sampling: <p>See <a href="http://data.bco-dmo.org/Fe_Synthesis/SOIREE/SOIREE_cruisereport.pdf">SOIREE Preliminary Voyage Report</a><br />
&nbsp;</p>
completed
Julie Hall
New Zealand National Institute of Water and Atmospheric Research
+64 4 386 0300
National Institute of Water & Atmospheric Research, Ltd. 301 Evans Bay Parade, Greta Point
Wellington
6021
New Zealand
j.hall@niwa.co.nz
pointOfContact
asNeeded
Dataset Version: 04Feb2010
Unknown
station
Day
Patch
depth
Bacteria
Biomass_Bacterial
Pico_Ekary
Biomass_Pico_Ekary
Bacterial_Prod
Biomass_Aut_Flag
Biomass_Het_Flag
MeanBiovolume_Aut_Flag
MeanBiovolume_Het_Flag
Aut_Flag
Het_Flag
Aut_to_Het
Non_Cil
Non_Lor_lt_20um
Biomass_Non_Lor_lt_20um
Non_Lor_gt_20um
Biomass_Non_Lor_gt_20um
Tintinnids
Biomass_Tintinnid
Biomass_Total_Ciliate
Total_Ciliates
Biomass_Total_Microzoop
date_UTC
date_local
lat
lon
timezone
time_UTC
time_local
theme
None, User defined
station
day of month
No BCO-DMO term
depth
date
date_local
latitude
longitude
time_zone
time of day
time_local
featureType
BCO-DMO Standard Parameters
61TG_3052
service
Deployment Activity
Southern Ocean
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Iron Synthesis
https://osprey.bco-dmo.org/program/2017
Iron Synthesis
The two main objectives of the Iron Synthesis program (SCOR Working Group proposal, 2005), are:
1. Data compilation: assembling a common open-access database of the in situ iron experiments, beginning with the first period (1993-2002; Ironex-1, Ironex-2, SOIREE, EisenEx, SEEDS-1; SOFeX, SERIES) where primary articles have already been published, to be followed by the 2004 experiments where primary articles are now in progress (EIFEX, SEEDS-2; SAGE, FeeP); similarly for the natural fertilizations S.O.JGOFS (1992), CROZEX (2004/2005) and KEOPS (2005).
2. Modeling and data synthesis of specific aspects of two or more such experiments for various topics such as physical mixing, phytoplankton productivity, overall ecosystem functioning, iron chemistry, CO2 budgeting, nutrient uptake ratios, DMS(P) processes, and combinations of these variables and processes.
SCOR Working Group proposal, 2005. "The Legacy of in situ Iron Enrichments: Data Compilation and Modeling".
http://www.scor-int.org/Working_Groups/wg131.htm
See also: SCOR Proceedings Vol. 42 Concepcion, Chile October 2006, pgs: 13-16 2.3.3 Working Group on The Legacy of in situ Iron Enrichments: Data Compilation and Modeling.
The first objective of the Iron Synthesis program involves a data recovery effort aimed at assembling a common, open-access database of data and metadata from a series of in-situ ocean iron fertilization experiments conducted between 1993 and 2005. Initially, funding for this effort is being provided by the Scientific Committee on Oceanic Research (SCOR) and the U.S. National Science Foundation (NSF).
Through the combined efforts of the principal investigators of the individual projects and the staff of Biological and Chemical Oceanography Data Management Office (BCO-DMO), data currently available primarily through individuals, disparate reports and data agencies, and in multiple formats, are being collected and prepared for addition to the BCO-DMO database from which they will be freely available to the community.
As data are contributed to the BCO-DMO office, they are organized into four overlapping categories:
1. Level 1, basic metadata
(e.g., description of project/study, general location, PI(s), participants);
2. Level 2, detailed metadata and basic shipboard data and routine ship's operations
(e.g., CTDs, underway measurements, sampling event logs);
3. Level 3, detailed metadata and data from specialized observations
(e.g., discrete observations, experimental results, rate measurements) and
4. Level 4, remaining datasets
(e.g., highest level of detailed data available from each study).
Collaboration with BCO-DMO staff began in March of 2008 and initial efforts have been directed toward basic project descriptions, levels 1 and 2 metadata and basic data, with detailed and more detailed data files being incorporated as they become available and are processed.
Related file
Program Documentation
The Iron Synthesis Program is funded jointly by the Scientific Committee on Oceanic Research (SCOR) and the U.S. National Science Foundation (NSF).
FeSynth
largerWorkCitation
program
Southern Ocean Iron Release Experiment
https://osprey.bco-dmo.org/project/2051
Southern Ocean Iron Release Experiment
<p>Project in the Southern Ocean aimed at maintaining a coherent patch of iron-enriched seawater for the duration of project and to interpret any iron-mediated effects on the patch by conducting measurements and performing experiments during this period of the project.</p>
<p>The Southern Ocean Iron RElease Experiment (SOIREE), was the first in situ iron fertilization experiment performed in the polar waters of the Southern Ocean. SOIREE was an interdisciplinary study involving participants from six countries, and took place in February 1999 south of the Polar Front in the Australasian-Pacific sector of the Southern Ocean.</p>
<p>Approximately 3800 kg of acidified FeSO4.7H2O and 165 g of the tracer sulphur hexafluoride (SF6) were added to a 65-m deep surface mixed layer over an area of ~50 km2. Initially, mean dissolved iron concentrations were ~2.7 nM, but decreased to ambient levels within days, requiring subsequent additions of 1550-1750 kg of acidified FeSO4.7H2O on days 3, 5 and 7 of the experiment.</p>
<p>During the 13-day site occupation, there were iron-mediated increases in phytoplankton growth rates, with marked increases in chlorophyll a (up to 2 µgl-1) and production rates (up to 1.3 gCm-2d-1). These resulted in subsequent changes in the pelagic ecosystem structure, and in the cycling of carbon, silica and sulphur, such as a 10% drawdown of surface CO2.</p>
<p>The SOIREE bloom persisted for >40 days following our departure from the site, as observed via <a href="http://data.bco-dmo.org/jg/serv/BCO/Fe_Synthesis/SOIREE/61TG_3052/SEAWIFS.html0" target="_blank"">SeaWiFS remotely sensed observations of Ocean Colour.</a></p>
<p><strong>BCO-DMO Note:</strong><br />
All original data and metadata provided on a CD-Rom accompanying the Deep-Sea Research II 48 (2001) volume. The CD-Rom contains the main SOIREE datasets and ancillary information including the pre-experiment 'desktop' database study for site-selection, and satellite images of the SOIREE bloom.<br />
© 2001 Elsevier Science Ltd. All rights reserved.</p>
<h3>Related files</h3>
<p><a href="http://data.bco-dmo.org/Fe_Synthesis/SOIREE/SOIREE_cruisereport.pdf">SOIREE Preliminary Voyage Report</a><br />
<a href="http://data.bco-dmo.org/Fe_Synthesis/SOIREE/Boyd_Law_2001_Intro.pdf">SOIREE Introduction and Summary, Deep-Sea Research II 48 (2001) 2425-2438</a><br />
<a href="http://data.bco-dmo.org/Fe_Synthesis/SOIREE/FeSyn_SOIREE_CruiseTrack_GoogleEarth.png" target="_blank"">SOIREE Cruise Track</a></p>
SOIREE
largerWorkCitation
project
eng; USA
biota
oceans
Southern Ocean
2010-02-04
Southern Ocean
0
BCO-DMO catalogue of parameters from Bacteria, Microzooplankton/Phytoplankton enumeration, production, grazing and growth from R/V Tangaroa cruise 61TG_3052 in the Southern Ocean in 1999 (SOIREE project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/19319.rdf
Name: station
Units: meters
Description: <p>Cruise Station Id</p>
http://lod.bco-dmo.org/id/dataset-parameter/19320.rdf
Name: Day
Units: integer
Description: <p>Experiment Day</p>
http://lod.bco-dmo.org/id/dataset-parameter/19321.rdf
Name: Patch
Units: text
Description: <p>Patch Location (In/Out)</p>
http://lod.bco-dmo.org/id/dataset-parameter/19322.rdf
Name: depth
Units: meters
Description: <p>Sample depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/19323.rdf
Name: Bacteria
Units: Cells ml-1
Description: <p>Bacteria</p>
http://lod.bco-dmo.org/id/dataset-parameter/19324.rdf
Name: Biomass_Bacterial
Units: ugC/ L
Description: <p>Biomass Bacterial</p>
http://lod.bco-dmo.org/id/dataset-parameter/19325.rdf
Name: Pico_Ekary
Units: Cells ml-1
Description: <p>Pico Ekary</p>
http://lod.bco-dmo.org/id/dataset-parameter/19326.rdf
Name: Biomass_Pico_Ekary
Units: ugC/ L
Description: <p>Biomass Pico Ekary</p>
http://lod.bco-dmo.org/id/dataset-parameter/19327.rdf
Name: Bacterial_Prod
Units: ug C l-1 h-1
Description: <p>Bacterial Prod</p>
http://lod.bco-dmo.org/id/dataset-parameter/19328.rdf
Name: Biomass_Aut_Flag
Units: ugC/ L
Description: <p>Biomass Aut Flag</p>
http://lod.bco-dmo.org/id/dataset-parameter/19329.rdf
Name: Biomass_Het_Flag
Units: ugC/ L
Description: <p>Biomass Het Flag</p>
http://lod.bco-dmo.org/id/dataset-parameter/19330.rdf
Name: MeanBiovolume_Aut_Flag
Units: um3
Description: <p>MeanBiovolume Aut Flag</p>
http://lod.bco-dmo.org/id/dataset-parameter/19331.rdf
Name: MeanBiovolume_Het_Flag
Units: um3
Description: <p>MeanBiovolume Het Flag</p>
http://lod.bco-dmo.org/id/dataset-parameter/19332.rdf
Name: Aut_Flag
Units: Cells ml-1
Description: <p>Aut Flag</p>
http://lod.bco-dmo.org/id/dataset-parameter/19333.rdf
Name: Het_Flag
Units: Cells ml-1
Description: <p>Het Flag</p>
http://lod.bco-dmo.org/id/dataset-parameter/19334.rdf
Name: Aut_to_Het
Units: dimensionless
Description: <p>Ratio of Aut to Het</p>
http://lod.bco-dmo.org/id/dataset-parameter/19335.rdf
Name: Non_Cil
Units: No's / L
Description: <p>Non Cil</p>
http://lod.bco-dmo.org/id/dataset-parameter/19336.rdf
Name: Non_Lor_lt_20um
Units: No's / L
Description: <p>Non Lor < 20um</p>
http://lod.bco-dmo.org/id/dataset-parameter/19337.rdf
Name: Biomass_Non_Lor_lt_20um
Units: ugC/ L
Description: <p>Biomass Non Lor < 20um</p>
http://lod.bco-dmo.org/id/dataset-parameter/19338.rdf
Name: Non_Lor_gt_20um
Units: No's / L
Description: <p>Non Lor > 20um</p>
http://lod.bco-dmo.org/id/dataset-parameter/19339.rdf
Name: Biomass_Non_Lor_gt_20um
Units: ugC/ L
Description: <p>Biomass Non Lor > 20um</p>
http://lod.bco-dmo.org/id/dataset-parameter/19340.rdf
Name: Tintinnids
Units: No's / L
Description: <p>Tintinnids</p>
http://lod.bco-dmo.org/id/dataset-parameter/19341.rdf
Name: Biomass_Tintinnid
Units: ugC/ L
Description: <p>Biomass Tintinnid</p>
http://lod.bco-dmo.org/id/dataset-parameter/19342.rdf
Name: Biomass_Total_Ciliate
Units: ugC/ L
Description: <p>Biomass Total Ciliate</p>
http://lod.bco-dmo.org/id/dataset-parameter/19343.rdf
Name: Total_Ciliates
Units: No's / L
Description: <p>Total Ciliates</p>
http://lod.bco-dmo.org/id/dataset-parameter/19344.rdf
Name: Biomass_Total_Microzoop
Units: ug/ L
Description: <p>Biomass Total Microzoop</p>
http://lod.bco-dmo.org/id/dataset-parameter/20867.rdf
Name: date_UTC
Units: YYYYMMDD
Description: <p>UTC Date</p>
http://lod.bco-dmo.org/id/dataset-parameter/20868.rdf
Name: date_local
Units: YYYYMMDD
Description: <p>local date</p>
http://lod.bco-dmo.org/id/dataset-parameter/20869.rdf
Name: lat
Units: decimal degrees
Description: <p>latitude, negative denotes South</p>
http://lod.bco-dmo.org/id/dataset-parameter/20870.rdf
Name: lon
Units: decimal degrees
Description: <p>longitude, negative denotes West</p>
http://lod.bco-dmo.org/id/dataset-parameter/20871.rdf
Name: timezone
Units: integer
Description: <p>local time zone, +/- from GMT</p>
http://lod.bco-dmo.org/id/dataset-parameter/20872.rdf
Name: time_UTC
Units: HHMM
Description: <p>UTC time</p>
http://lod.bco-dmo.org/id/dataset-parameter/20873.rdf
Name: time_local
Units: HHMM
Description: <p>local time</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
14846
https://datadocs.bco-dmo.org/file/m77kz4rFjAM8PG/microbiology.csv
microbiology.csv
Primary data file for dataset ID 2845
download
https://osprey.bco-dmo.org/dataset/2845/data/download
download
onLine
dataset
<p>See <a href="http://data.bco-dmo.org/Fe_Synthesis/SOIREE/SOIREE_cruisereport.pdf">SOIREE Preliminary Voyage Report</a><br />
&nbsp;</p>
Specified by the Principal Investigator(s)
<p>See <a href="http://data.bco-dmo.org/Fe_Synthesis/SOIREE/SOIREE_cruisereport.pdf">SOIREE Preliminary Voyage Report</a><br />
<br />
<strong>Bacteria enumeration</strong><br />
Bacterial abundance was determined by Flow Cytometry using a FACScan (15mW air-cooled<br />
argon-ion laser fixed at 488 nm) instrument (Becton Dickinson, Mountain View, Calif.)<br />
with CellQuest Vers. 3.1f software. The sheath fluid was 0.1 µm filtered sea water and<br />
the analysed volume was calculated using TrucountTM (Becton Dickinson, Mountain View, Calif.)<br />
beads as a tracer. The samples were frozen in liquid nitrogen and stained using SYBRII stain<br />
(Molecular Probes Inc.) at a concentration of 10-4 of stock solution and then incubated in<br />
the dark for 10-15 mins before being analysed following the methods described in Lebaron et. al., (1998).<br />
Just prior to analysis, 100ml of TrucountTM beads were added to each sample as a tracer.<br />
Samples were run on the low flow setting (~12 µl/min) with a minimum of 10,000 counts per sample.<br />
The bacterial population was identified using a FL1 (green light, 530/30 BP filter, log scale)<br />
vs FSC (Cell size, linear scale) scatter plot. FL1 was set to a voltage of 520 with an amp gain of 1.0.<br />
FSC was set to the E02 voltage setting with an amp gain of 7.37. Cell carbon for bacteria was estimated<br />
to be 12.4 fg C per cell as reported by Fukuda et .al., 1998). Flow cytometric counts of both bacteria<br />
were verified by direct counts using epifluorescent microscopy following Hobbie et. al., (1977).<br />
The variations between cytometric counts and microscopy counts were within the range reported by both<br />
Lebaron et. al., (1998) and Del Giorgio et. al., (1996).<br />
<br />
<strong>Eukaryotic picophytoplankton enumeration</strong><br />
Eukaryotic picophytoplankton abundance was determined by Flow Cytometry using a FACScan<br />
(15mW air-cooled argon-ion laser fixed at 488 nm) instrument (Becton Dickinson, Mountain View, Calif.)<br />
with CellQuest Vers. 3.1f software. The sheath fluid was 0.1 µm filtered sea water and the<br />
analysed volume was calculated using TrucountTM (Becton Dickinson, Mountain View, Calif.)<br />
beads as a tracer. The samples were frozen in liquid nitrogen (Lebaron et. al., 1998) and<br />
were thawed immediately before counting and 50ml of TrucountTM beads were added as a tracer.<br />
Samples were run on the FACScan at Hi flow setting (~60 µl/min) with a minimum of 1500 counts<br />
per sample following methods based on Corzo et. al. (1999) and Li (1989). Eukaryotic picophytoplankton<br />
numbers were then determined under FL1 vs FL2 (orange/red light, 585/42 BP filter). FL1 was<br />
set to a voltage of 520 with an amp gain of 1.0. FL2 was set to a voltage of 420 with an amp<br />
gain of 1.0. To remove background electronic noise FL2 was also set at a threshold of 52.<br />
Cell carbon for &lt;2 µm eukaryotic picophytoplankton was determined by assessing average spherical<br />
diameter by direct microscope observation using epifluorescence. This was then converted following<br />
Booth (1988) conversion for small phytoplankton (&lt;4 µm) using 220 fg C µm3 to yield a factor<br />
of 920 fg C per eukaryotic picophytoplankton. Flow cytometric counts of eukaryotic picophytoplankton<br />
were verified by direct counts using epifluorescent microscopy following Hall (1991). The variations<br />
between cytometric counts and microscopy counts were within the range reported by both Lebaron<br />
et. al., (1998) and Del Giorgio et. al., (1996).<br />
<br />
<strong>Bacterial Production</strong><br />
Heterotrophic bacterial productivity was measured using (methyl-3H) thymidine as detailed in<br />
Smith &amp; Hall (1997). Incubations were conducted at in situ surface temperatures. The extraction<br />
procedure followed Wicks &amp; Robarts (1987) modified TCA precipitate method, which involved rinsing<br />
the TCA precipitate with phenol-chloroform followed by ethanol. Tritium incorporation was determined<br />
with a liquid scintillation counter (LKB, 1217 Rackbeta) using OptiPhase HiSafe 3 (Wallac) as the<br />
scintillation fluor. Counts were corrected for quench by external standards. In order to estimate<br />
bacterial production we converted mol thymidine to g C using Fuhrman &amp; Azam's (1982) conversion<br />
factor of 2.4 x 1018 cells per mol thymidine incorporated, and Lee &amp; Fuhrman's (1987) estimate of<br />
20 x 10-15 g C cell -1.<br />
<br />
<strong>Nanoflagellate enumeration</strong><br />
Samples collected for nanoflagellate enumeration were size fractionated through a 20 µm nylon mesh.<br />
The filtrate was then fixed 1:1 with ice cold glutaraldehyde (2% final concentration) for 1 hour<br />
(Sanders et. al., 1989). Fixed samples were filtered onto prestained 0.8 µm black Nuclepore filters,<br />
stained for five minutes with 2 ml primulin, rinsed with 2 ml Tris HCL, mounted on slides and stored<br />
rozen (Bloem et. al., 1986). Nanoflagellates were counted under UV excitation using a Leica compound<br />
microscope (BP 450-490 nm excitation, LP 520 barrier filter, FT 510 dichromatic beam splitter).<br />
Nanophytoflagellates were differentiated by chlorophyll a fluorescing red under blue light excitation<br />
(BP 450-490 excitation, LP 515 barrier filter, RPK 510 dichromatic beam splitter). Forty randomly<br />
selected fields were counted per filter. Nanoflagellate biovolumes were calculated using dimensions<br />
and approximated geometric shape (Chang 1988). Biovolumes were calculated from measurements on a<br />
minimum of 200 cells, collected at 20 m for each sampling. Cell carbon for phyto and heterotrophic<br />
nanoflagellate biomass was estimated to be 0.24 pg C per µm-3 as reported by Verity et. al., (1992)<br />
for nanophytoflagellates.<br />
<br />
<strong>Ciliate enumeration</strong><br />
Samples for enumeration of ciliates were preserved in 1% Lugol's iodine. Samples were left to settle<br />
for 48 h and the supernatant removed. The remaining sample was transferred to a 25 ml Utermohl chamber.<br />
The microzooplankton were identified to genus where possible and enumerated using a Wild inverted<br />
microscope (James &amp; Hall, 1995) but with no differentiation of plastidic ciliates. Ciliate biomass<br />
was estimated from dimensions of 10 to 20 randomly chosen individuals of each taxon. The volumes<br />
were estimated from approximate geometric shapes and were converted to carbon biomass using a factor<br />
of 0.19 pg C µm -3 (Putt &amp; Stoecker 1989). The use of Lugol's iodine for preservation may have resulted<br />
in an underestimation of biomass due to cell shrinkage.<br />
<br />
<strong>Microzooplankton grazing rates and phytoplankton growth rates</strong><br />
Microzooplankton grazing rates and phytoplankton growth rates were determined using methods based on<br />
the dilution technique of Landry &amp; Hassett (1982), modified following the experimental protocols<br />
described in Gallegos et. al., (1996). Water for dilution was gravity filtered at 0.2 µm through a<br />
pre-rinsed Gelman SuporCapTM 100. Filtration of the dilution water required about 1 h. The total<br />
standing time between water collection and the start of the experiments was a maximum 2 h. In a<br />
set of 12 acid washed, 2.4-litre polycarbonate bottles, &lt;200 µm screened water was diluted with<br />
0.2 µm filtered water to concentrations of ~10%, 40%, 70% and 100% (i.e. undiluted). The screening<br />
of the water at 200 µm removed up to 40% of the phytoplankton particularly towards the end of the<br />
experiment when chains of large diatoms had formed. All bottles were then placed in an on deck<br />
incubator, with continuous sea water supply and covered with shade cloth that transmitted ~40% of<br />
the incident light, simulating ambient conditions. Incubations for all experiments were conducted<br />
in triplicate bottles for 24 h.<br />
<br />
The dilution factor for each bottle was calculated by taking sub-samples for &lt;200 µm chlorophyll a at<br />
T0 and measuring the actual percentage of &lt;200 µm chlorophyll a in each treatment. Size fractionated<br />
chlorophyll a and picophytoplankton sub-samples were also taken from experiments at T24 from all dilutions,<br />
but only from 100% undiluted water at T0. To determine growth and grazing rates on phytoplankton, size<br />
fractionated chlorophyll a and picophytoplankton at T0 were then estimated for each dilution using the<br />
calculated dilution. In addition, to determine growth rate of grazers, we measured heterotrophic<br />
nanoflagellate and ciliate in only 100% undiluted water at T0 and T24.<br />
<br />
Chlorophyll a was measured by filtering 500 mls of sample through a Whatman GF/F filter. Size<br />
fractioned chlorophyll a samples were collected using prefiltration at 20 and 2 µm. The filters<br />
were stored frozen until analysed spectrofluorometrically on a Perkin-Elmer LS 50B (Strickland &amp; Parsons 1972).<br />
<br />
<strong>BCO-DMO Processing Notes</strong><br />
Generated from original file Microbiology.xls provided on the<br />
Deep-Sea Research II 48 (2001) accompanying CD-Rom<br />
<br />
<strong>BCO-DMO Edits</strong><br />
- parameter names modified to conform to BCO-DMO convention<br />
- date reformatted to YYYYMMDD<br />
- Station Number changed to station<br />
- added 'T' to CTD Station number for compatibility with events in other spreadsheets<br />
- blank rows in original sheet removed<br />
- 'nd' added to blank cells or cells with '-' in original file<br />
- added Patch Location column and removed individual rows with 'Outside' and 'Inside'<br />
- date_UTC, time_UTC, date_local, time_local, timezone, lon and lat added from CTD sampling spreadsheet<br />
&nbsp;</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Cruise: 61TG_3052
61TG_3052
Community Standard Description
International Council for the Exploration of the Sea
R/V Tangaroa
vessel
61TG_3052
Philip W. Boyd
University of Otago
http://bcodata.whoi.edu/Fe_Synthesis/SOIREE/SOIREE_cruisereport.pdf
Report describing 61TG_3052
Community Standard Description
International Council for the Exploration of the Sea
R/V Tangaroa
vessel