http://lod.bco-dmo.org/id/dataset/2939
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2010-06-16
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Fluorometric chlorophyll from SCUFA underway sampling apparatus from R/V Melville cruise COOK19MV from the Southern Ocean, south of New Zealand in 2002 (SOFeX project)
2008-08-13
publication
2008-08-13
revision
BCO-DMO Linked Data URI
2008-08-13
creation
http://lod.bco-dmo.org/id/dataset/2939
Richard Barber
Duke University
principalInvestigator
Kenneth H. Coale
Moss Landing Marine Laboratories
principalInvestigator
Walker O. Smith
Virginia Institute of Marine Science
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Barber, R., Smith, W. O., Coale, K. H. (2008) Fluorometric chlorophyll from SCUFA underway sampling apparatus from R/V Melville cruise COOK19MV from the Southern Ocean, south of New Zealand in 2002 (SOFeX project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 13 August 2008) Version Date 2008-08-13 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/2939 [access date]
Fluorometric chlorophyll from SCUFA underway sampling apparatus Dataset Description: <p>Fluorometric chlorophyll from SCUFA underway sampling apparatus</p>
<p>When mapping, use Antarctic view (from 'Map Options' pulldown).</p> Methods and Sampling: <pre>
<strong>dates:</strong> 19 January 2002 to 20 February 2002 (20020119-20020220)
<strong>location:</strong> N: -46.6233 S: -66.3606 W: 179.7417 E: -178.8283
<strong>project/cruise:</strong> SOFeX/MV
</pre>
<p><strong>Contact:</strong> Anna Hilting (Duke University Marine Laboratory)</p>
<p><strong>R/V Melville Extracted Chlorophyll Methodology</strong><br />
<br />
Please direct questions to Sara Tanner (tanner@mlml.calstate.edu) or Jodi Brewster (jbrewster@mlml.calstate.edu)<br />
<br />
Water samples were collected from 12 depths on the CTD Rosette and 8 depths on the TM Rosette. The TM Rosette depths were chosen at the 100, 45, 30, 16, 10, 5, 1, and 0.1 percent light levels (so phytoplankton production can be related to phytoplankton biomass) (Evans et al 1987). The CTD also had 2 more depths scattered between .1 and 100 percent and one each at 200m and 300m. The water from the CTD and TM rosettes was collected using opaque brown bottles in 250, 500, 1000, and 2000 ml or white 100 ml bottles. The differing volumes depended upon the depth of the sample and whether the samples were taken within the patch or not. Sampling from the TM Rosette was done with gloves. Each bottle was rinsed three times with the sample water before filling to the neck of the bottle.<br />
<br />
A Whatman G/FF glass Fiber Filter, (~0.7um) Polycarbonate 5 um filter, or Polycarbonate 20 micron filter was placed in a 25 mm diameter Gelman filter holder. Water was pumped through the filter, being careful the vacuum pressure did not get above 6 psi to avoid cell lyse. After filtration, the vacuum was turned off and the filter was added with forceps to a tube filled with 8 ml of 90% acetone. The tube was labeled and stored in a freezer for a minimum of 24 hours.<br />
<br />
After the minimum 24 hours extraction time, the filter was removed from the tube and the tube was wiped down with Chem Wipes. The fluorescence of the chlorophyll extracts were read on a 10AU Turner Designs fluorometer. Two drops of 10 % HCl was added and the fluorescence was reread and recorded again. The "before" and "after" readings were plugged into equation chl-a = K * (Rb-Ra) * (vol ext/vol filtered)*dil to calculate chlorophyll a values.<br />
<br />
A standard made from Sigma Chl-a in 90% acetone was calibrated on a spectrophotometer and used to calibrate the fluorometer at the beginning, mid and end of the cruise. Due to the fact the fluorometer drifted both ± according to the solid standard, and a high correlation was found between the low solid standard and the calibration curve, Chlorophyll-a values were corrected using the ratio of the low solid standard.</p>
<p><strong>PI Notes</strong><br />
from Richard Barber (rbarber@duke.edu) and Anna Hilting (ahilting@duke.edu)<br />
<br />
Chlorophyll a was determined by fluorometric methods. Fresh samples were extracted in 90% acetone at -20 degrees C for 24-30 h (Venrick and Hayward, 1984) and quantified using a Turner Designs fluorometer (Holm-Hansen et al., 1965; Lorenzen, 1966). Contact A. Hilting (Duke) for information.<br />
<br />
These data have been edited for quality control but will be processed further for size-fraction analysis and integration using the Morel Model. See Barber et al., 1997 and Hiscock et al., 2002. Incubated depth will be calculated using the Morel model and added later.</p>
<p><strong>SCUFA Underway Chlorophyll Survey Data</strong><br />
<br />
<strong>OCB DMO Note:</strong> Worked primarily with 'calc' worksheet from chlaSCUFA.xls. Combined elements from MelvilleChlorophyll.xls. Included data for cast_scufa U059 and 20 micron filter SCUFA samples in cast_scufa range S159-S179 from MelvilleChlorophyll.xls as well. A summary worksheet of corrected chlorophyll-a and SCUFA fluorescence data 'Chlorophyll' is found in chlaSCUFA.xls as well as a worksheet on blanks, 'Blanks'. Placement of cast_scufa U059 based on placement in cruise event log. U059 values for date, lon, lat, and notes added from cruise event log.</p>
<p><strong>SCUFA</strong> or Self-Contained Underwater Fluorescence Apparatus<br />
<a href="http://ocb.whoi.edu/SOFeX/PI-NOTES/scufa_brochure.pdf">SCUFA brochure</a> from Turner Designs</p>
<p><a href="http://ocb.whoi.edu/SOFeX/PI-NOTES/data_orig/chlaSCUFA.xls">Original Excel file</a><br />
of SCUFA chlorophyll calibration work for underway surface extracted chlorophyll</p>
completed
Richard Barber
Duke University
USA
rbarber@duke.edu
pointOfContact
Kenneth H. Coale
Moss Landing Marine Laboratories
USA
coale@mlml.calstate.edu
pointOfContact
Walker O. Smith
Virginia Institute of Marine Science
(804) 684-7709
P.O. Box 1346 Rt. 1208 Greate Rd.
Gloucester Point
VA
23062-1346
USA
wos@vims.edu
pointOfContact
asNeeded
Dataset Version: 13 August 2008
Unknown
cast_scufa
sample
date
time
time_obs
lon
lat
filt
filt_rig
person
fluor
chl_corr
phaeo
rb
ra
chl_to_ph
phaeo_calc
chl_calc
vol_filt
vol_extract
dil
door
blank_lo
blank_seq
filename
notes
Self-Contained Underwater Fluorescence Apparatus
theme
None, User defined
No BCO-DMO term
sample identification
date
time of day
longitude
latitude
investigator
fluorescence
total phaeopigment
volume of water filtered
featureType
BCO-DMO Standard Parameters
Self-Contained Underwater Fluorescence Apparatus
instrument
BCO-DMO Standard Instruments
COOK19MV
service
Deployment Activity
Southern Ocean
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Ocean Carbon and Biogeochemistry
http://us-ocb.org/
Ocean Carbon and Biogeochemistry
The Ocean Carbon and Biogeochemistry (OCB) program focuses on the ocean's role as a component of the global Earth system, bringing together research in geochemistry, ocean physics, and ecology that inform on and advance our understanding of ocean biogeochemistry. The overall program goals are to promote, plan, and coordinate collaborative, multidisciplinary research opportunities within the U.S. research community and with international partners. Important OCB-related activities currently include: the Ocean Carbon and Climate Change (OCCC) and the North American Carbon Program (NACP); U.S. contributions to IMBER, SOLAS, CARBOOCEAN; and numerous U.S. single-investigator and medium-size research projects funded by U.S. federal agencies including NASA, NOAA, and NSF.
The scientific mission of OCB is to study the evolving role of the ocean in the global carbon cycle, in the face of environmental variability and change through studies of marine biogeochemical cycles and associated ecosystems.
The overarching OCB science themes include improved understanding and prediction of: 1) oceanic uptake and release of atmospheric CO2 and other greenhouse gases and 2) environmental sensitivities of biogeochemical cycles, marine ecosystems, and interactions between the two.
The OCB Research Priorities (updated January 2012) include: ocean acidification; terrestrial/coastal carbon fluxes and exchanges; climate sensitivities of and change in ecosystem structure and associated impacts on biogeochemical cycles; mesopelagic ecological and biogeochemical interactions; benthic-pelagic feedbacks on biogeochemical cycles; ocean carbon uptake and storage; and expanding low-oxygen conditions in the coastal and open oceans.
OCB
largerWorkCitation
program
Iron Synthesis
https://osprey.bco-dmo.org/program/2017
Iron Synthesis
The two main objectives of the Iron Synthesis program (SCOR Working Group proposal, 2005), are:
1. Data compilation: assembling a common open-access database of the in situ iron experiments, beginning with the first period (1993-2002; Ironex-1, Ironex-2, SOIREE, EisenEx, SEEDS-1; SOFeX, SERIES) where primary articles have already been published, to be followed by the 2004 experiments where primary articles are now in progress (EIFEX, SEEDS-2; SAGE, FeeP); similarly for the natural fertilizations S.O.JGOFS (1992), CROZEX (2004/2005) and KEOPS (2005).
2. Modeling and data synthesis of specific aspects of two or more such experiments for various topics such as physical mixing, phytoplankton productivity, overall ecosystem functioning, iron chemistry, CO2 budgeting, nutrient uptake ratios, DMS(P) processes, and combinations of these variables and processes.
SCOR Working Group proposal, 2005. "The Legacy of in situ Iron Enrichments: Data Compilation and Modeling".
http://www.scor-int.org/Working_Groups/wg131.htm
See also: SCOR Proceedings Vol. 42 Concepcion, Chile October 2006, pgs: 13-16 2.3.3 Working Group on The Legacy of in situ Iron Enrichments: Data Compilation and Modeling.
The first objective of the Iron Synthesis program involves a data recovery effort aimed at assembling a common, open-access database of data and metadata from a series of in-situ ocean iron fertilization experiments conducted between 1993 and 2005. Initially, funding for this effort is being provided by the Scientific Committee on Oceanic Research (SCOR) and the U.S. National Science Foundation (NSF).
Through the combined efforts of the principal investigators of the individual projects and the staff of Biological and Chemical Oceanography Data Management Office (BCO-DMO), data currently available primarily through individuals, disparate reports and data agencies, and in multiple formats, are being collected and prepared for addition to the BCO-DMO database from which they will be freely available to the community.
As data are contributed to the BCO-DMO office, they are organized into four overlapping categories:
1. Level 1, basic metadata
(e.g., description of project/study, general location, PI(s), participants);
2. Level 2, detailed metadata and basic shipboard data and routine ship's operations
(e.g., CTDs, underway measurements, sampling event logs);
3. Level 3, detailed metadata and data from specialized observations
(e.g., discrete observations, experimental results, rate measurements) and
4. Level 4, remaining datasets
(e.g., highest level of detailed data available from each study).
Collaboration with BCO-DMO staff began in March of 2008 and initial efforts have been directed toward basic project descriptions, levels 1 and 2 metadata and basic data, with detailed and more detailed data files being incorporated as they become available and are processed.
Related file
Program Documentation
The Iron Synthesis Program is funded jointly by the Scientific Committee on Oceanic Research (SCOR) and the U.S. National Science Foundation (NSF).
FeSynth
largerWorkCitation
program
Southern Ocean Iron Experiment
http://www.mbari.org/expeditions/SOFeX2002/
Southern Ocean Iron Experiment
<p>Before he passed away in 1993, John Martin suggested that an increase in the flow of iron-rich dust to the ocean causes phytoplankton (single celled algae) to grow. The increased photosynthesis removes carbon dioxide from surface waters as the algae create biomass. This carbon dioxide is replaced by carbon dioxide gas that flows into the sea from the atmosphere. Reduced carbon dioxide in the atmosphere cools the planet (CO2 is a greenhouse gas that warms the earth). The results of this work, funded by the National Science Foundation, the Department of Energy, and the US Coast Guard, will be a much better understanding of how biological processes may regulate climate. (see Related Info: Fe cycle)</p>
<p>A direct test of the 'Martin Hypothesis' that trace concentrations of Fe are responsible for phytoplankton's ability to grow by direct experimental addition of Fe to the surface waters. Consequently the distribution of bioavailable Fe in the surface waters determines large geographical areas primary production and the following flux of fixed organic matter to the deep sea. The aim of the SOFeX project is to investigate the effects of iron fertilization on the productivity of the Southern Ocean. The results of this work will contribute significantly to our understanding of important biogeochemical processes which bear directly on the global carbon cycle, atmospheric carbon dioxide concentration, and climate control.</p>
<p>The SOFeX-N and SOFeX-S designations are sometimes used to distinguish between two iron enriched patches - one in low silicate waters north of the polar front (SOFEX-N), and the other in high silicate waters south of the polar front (SOFEX-S). All three ships, Melville (MV), Revelle (RR) and Polar Star (PS), worked in SOFEX-S, but only the Revelle and Melville worked in the SOFeX N patch and shuttled between the two patches.</p>
SOFeX
largerWorkCitation
project
eng; USA
oceans
Southern Ocean
2008-08-13
Southern Ocean, south of New Zealand
0
BCO-DMO catalogue of parameters from Fluorometric chlorophyll from SCUFA underway sampling apparatus from R/V Melville cruise COOK19MV from the Southern Ocean, south of New Zealand in 2002 (SOFeX project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/15136.rdf
Name: cast_scufa
Units: alphanumeric
Description: <p>SCUFA cast number</p>
http://lod.bco-dmo.org/id/dataset-parameter/15137.rdf
Name: sample
Units: dimensionless
Description: <p>sample number, coincides with other samples taken during evening transects</p>
http://lod.bco-dmo.org/id/dataset-parameter/15138.rdf
Name: date
Units: yyyymmdd
Description: <p>sample date from SCUFA file (GMT)</p>
http://lod.bco-dmo.org/id/dataset-parameter/15139.rdf
Name: time
Units: hhmmss
Description: <p>sample time from SCUFA file (GMT)</p>
http://lod.bco-dmo.org/id/dataset-parameter/15140.rdf
Name: time_obs
Units: hhmm
Description: <p>time from observer taking Chlorophyll sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/15141.rdf
Name: lon
Units: decimal degrees
Description: <p>longitude; negative denotes West</p>
http://lod.bco-dmo.org/id/dataset-parameter/15142.rdf
Name: lat
Units: decimal degrees
Description: <p>latitude; negative denotes South</p>
http://lod.bco-dmo.org/id/dataset-parameter/15143.rdf
Name: filt
Units: microns
Description: <p>filter size</p>
http://lod.bco-dmo.org/id/dataset-parameter/15144.rdf
Name: filt_rig
Units: dimensionless
Description: <p>filter rig</p>
http://lod.bco-dmo.org/id/dataset-parameter/15145.rdf
Name: person
Units: dimensionless
Description: <p>scientist responsible for data from an event</p>
http://lod.bco-dmo.org/id/dataset-parameter/15146.rdf
Name: fluor
Units: dimensionless
Description: <p>fluorescence</p>
http://lod.bco-dmo.org/id/dataset-parameter/15147.rdf
Name: chl_corr
Units: mg/m^3
Description: <p>corrected chlorophyll-a, K*(rb-ra)*(vol_extract/vol_filt)*dil*(7.87/blank_lo), K=1.75</p>
http://lod.bco-dmo.org/id/dataset-parameter/15148.rdf
Name: phaeo
Units: mg/m^3
Description: <p>phaeophytin ??, K*phaeo_calc*(8.0/vol_filt)*dil, K=1.75</p>
http://lod.bco-dmo.org/id/dataset-parameter/15149.rdf
Name: rb
Units: dimensionless
Description: <p>fluorescence, reading before</p>
http://lod.bco-dmo.org/id/dataset-parameter/15150.rdf
Name: ra
Units: dimensionless
Description: <p>fluorescence, reading after</p>
http://lod.bco-dmo.org/id/dataset-parameter/15151.rdf
Name: chl_to_ph
Units: dimensionless
Description: <p>chl/ph, rb/ra</p>
http://lod.bco-dmo.org/id/dataset-parameter/15152.rdf
Name: phaeo_calc
Units: dimensionless
Description: <p>calculated phaeo, (ra*tao)-rb, tao=2.069</p>
http://lod.bco-dmo.org/id/dataset-parameter/15153.rdf
Name: chl_calc
Units: dimensionless
Description: <p>calculated chlorophyll-a, K*(rb-ra)*(vol_extract/vol_filt)*dil, K=1.75</p>
http://lod.bco-dmo.org/id/dataset-parameter/15154.rdf
Name: vol_filt
Units: mls
Description: <p>filtered volume</p>
http://lod.bco-dmo.org/id/dataset-parameter/15155.rdf
Name: vol_extract
Units: mls
Description: <p>extracted volume</p>
http://lod.bco-dmo.org/id/dataset-parameter/15156.rdf
Name: dil
Units: dimensionless
Description: <p>dilution factor??</p>
http://lod.bco-dmo.org/id/dataset-parameter/15157.rdf
Name: door
Units: unknown ??
Description: <p>unknown ??</p>
http://lod.bco-dmo.org/id/dataset-parameter/15158.rdf
Name: blank_lo
Units: unknown ??
Description: <p>low blank</p>
http://lod.bco-dmo.org/id/dataset-parameter/15159.rdf
Name: blank_seq
Units: dimensionless
Description: <p>blank sequence</p>
http://lod.bco-dmo.org/id/dataset-parameter/15160.rdf
Name: filename
Units: dimensionless
Description: <p>SCUFA file for continual fluorescence sample log</p>
http://lod.bco-dmo.org/id/dataset-parameter/15161.rdf
Name: notes
Units: dimensionless
Description: <p>comments</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
44670
https://datadocs.bco-dmo.org/file/WWW1EJKsxmGlBQ/chlorophyll_uway.csv
chlorophyll_uway.csv
Primary data file for dataset ID 2939
download
https://osprey.bco-dmo.org/dataset/2939/data/download
download
onLine
dataset
<pre>
<strong>dates:</strong> 19 January 2002 to 20 February 2002 (20020119-20020220)
<strong>location:</strong> N: -46.6233 S: -66.3606 W: 179.7417 E: -178.8283
<strong>project/cruise:</strong> SOFeX/MV
</pre>
<p><strong>Contact:</strong> Anna Hilting (Duke University Marine Laboratory)</p>
<p><strong>R/V Melville Extracted Chlorophyll Methodology</strong><br />
<br />
Please direct questions to Sara Tanner (tanner@mlml.calstate.edu) or Jodi Brewster (jbrewster@mlml.calstate.edu)<br />
<br />
Water samples were collected from 12 depths on the CTD Rosette and 8 depths on the TM Rosette. The TM Rosette depths were chosen at the 100, 45, 30, 16, 10, 5, 1, and 0.1 percent light levels (so phytoplankton production can be related to phytoplankton biomass) (Evans et al 1987). The CTD also had 2 more depths scattered between .1 and 100 percent and one each at 200m and 300m. The water from the CTD and TM rosettes was collected using opaque brown bottles in 250, 500, 1000, and 2000 ml or white 100 ml bottles. The differing volumes depended upon the depth of the sample and whether the samples were taken within the patch or not. Sampling from the TM Rosette was done with gloves. Each bottle was rinsed three times with the sample water before filling to the neck of the bottle.<br />
<br />
A Whatman G/FF glass Fiber Filter, (~0.7um) Polycarbonate 5 um filter, or Polycarbonate 20 micron filter was placed in a 25 mm diameter Gelman filter holder. Water was pumped through the filter, being careful the vacuum pressure did not get above 6 psi to avoid cell lyse. After filtration, the vacuum was turned off and the filter was added with forceps to a tube filled with 8 ml of 90% acetone. The tube was labeled and stored in a freezer for a minimum of 24 hours.<br />
<br />
After the minimum 24 hours extraction time, the filter was removed from the tube and the tube was wiped down with Chem Wipes. The fluorescence of the chlorophyll extracts were read on a 10AU Turner Designs fluorometer. Two drops of 10 % HCl was added and the fluorescence was reread and recorded again. The "before" and "after" readings were plugged into equation chl-a = K * (Rb-Ra) * (vol ext/vol filtered)*dil to calculate chlorophyll a values.<br />
<br />
A standard made from Sigma Chl-a in 90% acetone was calibrated on a spectrophotometer and used to calibrate the fluorometer at the beginning, mid and end of the cruise. Due to the fact the fluorometer drifted both ± according to the solid standard, and a high correlation was found between the low solid standard and the calibration curve, Chlorophyll-a values were corrected using the ratio of the low solid standard.</p>
<p><strong>PI Notes</strong><br />
from Richard Barber (rbarber@duke.edu) and Anna Hilting (ahilting@duke.edu)<br />
<br />
Chlorophyll a was determined by fluorometric methods. Fresh samples were extracted in 90% acetone at -20 degrees C for 24-30 h (Venrick and Hayward, 1984) and quantified using a Turner Designs fluorometer (Holm-Hansen et al., 1965; Lorenzen, 1966). Contact A. Hilting (Duke) for information.<br />
<br />
These data have been edited for quality control but will be processed further for size-fraction analysis and integration using the Morel Model. See Barber et al., 1997 and Hiscock et al., 2002. Incubated depth will be calculated using the Morel model and added later.</p>
<p><strong>SCUFA Underway Chlorophyll Survey Data</strong><br />
<br />
<strong>OCB DMO Note:</strong> Worked primarily with 'calc' worksheet from chlaSCUFA.xls. Combined elements from MelvilleChlorophyll.xls. Included data for cast_scufa U059 and 20 micron filter SCUFA samples in cast_scufa range S159-S179 from MelvilleChlorophyll.xls as well. A summary worksheet of corrected chlorophyll-a and SCUFA fluorescence data 'Chlorophyll' is found in chlaSCUFA.xls as well as a worksheet on blanks, 'Blanks'. Placement of cast_scufa U059 based on placement in cruise event log. U059 values for date, lon, lat, and notes added from cruise event log.</p>
<p><strong>SCUFA</strong> or Self-Contained Underwater Fluorescence Apparatus<br />
<a href="http://ocb.whoi.edu/SOFeX/PI-NOTES/scufa_brochure.pdf">SCUFA brochure</a> from Turner Designs</p>
<p><a href="http://ocb.whoi.edu/SOFeX/PI-NOTES/data_orig/chlaSCUFA.xls">Original Excel file</a><br />
of SCUFA chlorophyll calibration work for underway surface extracted chlorophyll</p>
from Cruise: COOK19MV <pre>
<b>dates:</b> 19 January 2002 to 20 February 2002 (20020119-20020220)
<b>location:</b> N: -46.6233 S: -66.3606 W: 179.7417 E: -178.8283
<b>project/cruise:</b> SOFeX/MV
</pre>
<p><strong>Contact:</strong> Anna Hilting (Duke University Marine Laboratory)</p>
<p><strong>R/V Melville Extracted Chlorophyll Methodology</strong><br>
<br>
Please direct questions to Sara Tanner (tanner@mlml.calstate.edu) or Jodi Brewster (jbrewster@mlml.calstate.edu)<br>
<br>
Water samples were collected from 12 depths on the CTD Rosette and 8
depths on the TM Rosette. The TM Rosette depths were chosen at the 100,
45, 30, 16, 10, 5, 1, and 0.1 percent light levels (so phytoplankton
production can be related to phytoplankton biomass) (Evans et al 1987).
The CTD also had 2 more depths scattered between .1 and 100 percent and
one each at 200m and 300m. The water from the CTD and TM rosettes was
collected using opaque brown bottles in 250, 500, 1000, and 2000 ml or
white 100 ml bottles. The differing volumes depended upon the depth of
the sample and whether the samples were taken within the patch or not.
Sampling from the TM Rosette was done with gloves. Each bottle was
rinsed three times with the sample water before filling to the neck of
the bottle.<br>
<br>
A Whatman G/FF glass Fiber Filter, (~0.7um) Polycarbonate 5 um filter, or
Polycarbonate 20 micron filter was placed in a 25 mm diameter Gelman filter
holder. Water was pumped through the filter, being careful the vacuum
pressure did not get above 6 psi to avoid cell lyse. After filtration, the
vacuum was turned off and the filter was added with forceps to a tube
filled with 8 ml of 90% acetone. The tube was labeled and stored in a
freezer for a minimum of 24 hours. <br>
<br>
After the minimum 24 hours extraction time, the filter was removed from
the tube and the tube was wiped down with Chem Wipes. The fluorescence of
the chlorophyll extracts were read on a 10AU Turner Designs fluorometer.
Two drops of 10 % HCl was added and the fluorescence was reread and
recorded again. The "before" and "after" readings were plugged into
equation chl-a = K * (Rb-Ra) * (vol ext/vol filtered)*dil to calculate
chlorophyll a values. <br>
<br>
A standard made from Sigma Chl-a in 90% acetone was calibrated on a
spectrophotometer and used to calibrate the fluorometer at the beginning,
mid and end of the cruise. Due to the fact the fluorometer drifted both ±
according to the solid standard, and a high correlation was found between
the low solid standard and the calibration curve, Chlorophyll-a values were
corrected using the ratio of the low solid standard.
</p>
<p><strong>PI Notes</strong> <br>
from Richard Barber (rbarber@duke.edu) and Anna Hilting (ahilting@duke.edu)
<br><br>
Chlorophyll a was determined by fluorometric methods.
Fresh samples were extracted in 90% acetone at -20 degrees C for 24-30 h (Venrick and
Hayward, 1984) and quantified using a Turner Designs fluorometer (Holm-Hansen et al.,
1965; Lorenzen, 1966).
Contact A. Hilting (Duke) for information.
<br><br>
These data have been edited for quality control but will be processed further for
size-fraction analysis and integration using the Morel Model. See Barber et al., 1997 and
Hiscock et al., 2002. Incubated depth will be calculated using the Morel model and added later.
</p>
<p><strong>SCUFA Underway Chlorophyll Survey Data</strong><br>
<br>
<strong>OCB DMO Note:</strong> Worked primarily with 'calc' worksheet from chlaSCUFA.xls. Combined elements
from MelvilleChlorophyll.xls. Included data for cast_scufa U059 and 20 micron filter SCUFA samples in
cast_scufa range S159-S179 from MelvilleChlorophyll.xls as well. A summary worksheet of
corrected chlorophyll-a and SCUFA fluorescence data 'Chlorophyll' is found in chlaSCUFA.xls as well
as a worksheet on blanks, 'Blanks'. Placement of cast_scufa U059 based on placement in cruise event log.
U059 values for date, lon, lat, and notes added from cruise event log.
</p>
<p><strong>SCUFA</strong> or Self-Contained Underwater Fluorescence Apparatus<br>
<a href="http://ocb.whoi.edu/SOFeX/PI-NOTES/scufa_brochure.pdf">SCUFA brochure</a> from Turner Designs</p>
<p><a href="http://ocb.whoi.edu/SOFeX/PI-NOTES/data_orig/chlaSCUFA.xls">Original Excel file</a><br>
of SCUFA chlorophyll calibration work for underway surface extracted chlorophyll</p>
Specified by the Principal Investigator(s)
<pre>
Change history:
070423: downloaded original data (chlaSCUFA.xls) and original zipped data (raw_SCUFA.zip)
from SOFeX project data website and extracted data
080813: added to OCB database by Cyndy Chandler, OCB DMO, (cchandler@whoi.edu)
</pre>
<p>13 August 2008: Prepared for OCB data system by Dave DuBois (WHOI) Cyndy Chandler, OCB DMO (WHOI) from documentation contributed by originating PI, data analysts and technicians.</p>
<p><strong>Original Excel file downloaded from MBARI:</strong> <a href="http://ocb.whoi.edu/SOFeX/PI-NOTES/data_orig/chlaSCUFA.xls" title="original file">copy of original Excel file</a></p>
<p><strong>Embedded comments: </strong><br />
from original Excel data file: 'Cast' U059 and column 'Chl (mg Chl m-3)'<br />
embedded comment "ahilting: error =.08*dilution=.16 ug"</p>
<p><strong>PI notes regarding Chlorophyll samples from SCUFA flow-thru: </strong><br />
N is sample number, coincides with other samples taken during evening transects. Date and Time GMT from SCUFA file. Obs. Time from Observer taking Chlorophyll sample. The Filename denotes the SCUFA file that continual fluorescence was logged for cholorophyll sample. If you have any questions, please contact Jodi Brewster (jbrewster@mlml.calstate.edu) or Sara Tanner (tanner@mlml.calstate.edu).<br />
<br />
For underway Survey data, please note that the Year Day and patch day values do not agree with Hiscock's event log, nor do most of the 'Landry' Cast Types, so integration of these data with other types from this cruise will be difficult without further work.</p>
from Cruise: COOK19MV <pre>
Change history:
070423: downloaded original data (chlaSCUFA.xls) and original zipped data (raw_SCUFA.zip)
from SOFeX project data website and extracted data
080813: added to OCB database by Cyndy Chandler, OCB DMO, (cchandler@whoi.edu)
</pre>
<p class="text">
13 August 2008: Prepared for OCB data system by Dave DuBois (WHOI) Cyndy Chandler, OCB DMO (WHOI) from documentation contributed by originating PI, data analysts and technicians.<br>
</p>
<p><strong>Original Excel file downloaded from MBARI:</strong>
<a href="http://ocb.whoi.edu/SOFeX/PI-NOTES/data_orig/chlaSCUFA.xls" title="original file">copy of original Excel file</a></p>
<p><strong>Embedded comments: </strong>
<br>
from original Excel data file: 'Cast' U059 and column 'Chl (mg Chl m-3)' <br>
embedded comment "ahilting: error =.08*dilution=.16 ug"
</p>
<p><strong>PI notes regarding Chlorophyll samples from SCUFA flow-thru: </strong>
<br>
N is sample number, coincides with other samples taken during evening transects.
Date and Time GMT from SCUFA file. Obs. Time from Observer taking Chlorophyll sample.
The Filename denotes the SCUFA file that continual fluorescence was logged for cholorophyll sample.
If you have any questions, please contact Jodi Brewster (jbrewster@mlml.calstate.edu) or Sara Tanner (tanner@mlml.calstate.edu).
<br><br>
For underway Survey data,
please note that the Year Day and patch day values do not agree with Hiscock's event log, nor do most of
the 'Landry' Cast Types, so integration of these data with other types from this cruise will be difficult without further work.</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Self-Contained Underwater Fluorescence Apparatus
Self-Contained Underwater Fluorescence Apparatus
PI Supplied Instrument Name: Self-Contained Underwater Fluorescence Apparatus Instrument Name: Self-Contained Underwater Fluorescence Apparatus Instrument Short Name:SCUFA Instrument Description: The Self-Contained Underwater Fluorescence Apparatus (SCUFA)
is a type of Submersible Fluorometer available from Turner Designs that can be deployed in either moored or profiling mode (SCUFA brochure). Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0189/
Cruise: COOK19MV
COOK19MV
R/V Melville
Community Standard Description
International Council for the Exploration of the Sea
R/V Melville
vessel
COOK19MV
Kenneth H. Coale
Moss Landing Marine Laboratories
http://ocb.whoi.edu/SOFeX/CRUISES/proj_description.pdf
Report describing COOK19MV
R/V Melville
Community Standard Description
International Council for the Exploration of the Sea
R/V Melville
vessel