<?xml version="1.0" encoding="utf-8" ?><gmi:MI_Metadata
  xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance"
  xmlns:gco="http://www.isotc211.org/2005/gco"
  xmlns:gfc="http://www.isotc211.org/2005/gfc"
  xmlns:gmd="http://www.isotc211.org/2005/gmd"
  xmlns:xlink="http://www.w3.org/1999/xlink"
  xmlns:srv="http://www.isotc211.org/2005/srv"
  xmlns:gml="http://www.opengis.net/gml/3.2"
  xmlns:gsr="http://www.isotc211.org/2005/gsr"
  xmlns:gss="http://www.isotc211.org/2005/gss"
  xmlns:gts="http://www.isotc211.org/2005/gts"
  xmlns:gmi="http://www.isotc211.org/2005/gmi"
  xmlns:gmx="http://www.isotc211.org/2005/gmx"
  xsi:schemaLocation="http://www.isotc211.org/2005/gmi https://data.noaa.gov/resources/iso19139/schema.xsd">
  <gmd:fileIdentifier>
    <gco:CharacterString>http://lod.bco-dmo.org/id/dataset/2964</gco:CharacterString>
  </gmd:fileIdentifier>
  <gmd:language>
    <gco:CharacterString>eng; USA</gco:CharacterString>
  </gmd:language>
  <gmd:characterSet>
    <gmd:MD_CharacterSetCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#MD_CharacterSetCode" codeListValue="utf8">utf8</gmd:MD_CharacterSetCode>
  </gmd:characterSet>
  <gmd:hierarchyLevel>
    <gmd:MD_ScopeCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#MD_ScopeCode" codeListValue="dataset" codeSpace="005">dataset</gmd:MD_ScopeCode>
  </gmd:hierarchyLevel>
  <gmd:hierarchyLevelName>
    <gco:CharacterString>Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project</gco:CharacterString>
  </gmd:hierarchyLevelName>
  <gmd:contact>
    <gmd:CI_ResponsibleParty>
  <gmd:organisationName>
    <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/affiliation/191.rdf" xlink:actuate="onRequest">Biological and Chemical Oceanography Data Management Office (BCO-DMO)</gmx:Anchor>
  </gmd:organisationName>
  <gmd:contactInfo>
    <gmd:CI_Contact>
		  <gmd:phone>
		    <gmd:CI_Telephone>
				  <gmd:voice>
				    <gco:CharacterString>Unavailable</gco:CharacterString>
				  </gmd:voice>
				  <gmd:facsimile>
				    <gco:CharacterString>508-289-2009</gco:CharacterString>
				  </gmd:facsimile>
				</gmd:CI_Telephone>
		  </gmd:phone>
		  <gmd:address>
		    <gmd:CI_Address>
				  <gmd:deliveryPoint>
				    <gco:CharacterString>WHOI MS#36</gco:CharacterString>
				  </gmd:deliveryPoint>
				  <gmd:city>
				    <gco:CharacterString>Woods Hole</gco:CharacterString>
				  </gmd:city>
				  <gmd:administrativeArea>
				    <gco:CharacterString>MA</gco:CharacterString>
				  </gmd:administrativeArea>
				  <gmd:postalCode>
				    <gco:CharacterString>02543</gco:CharacterString>
				  </gmd:postalCode>
				  <gmd:country>
				    <gco:CharacterString>USA</gco:CharacterString>
				  </gmd:country>
				  <gmd:electronicMailAddress>
				    <gco:CharacterString>info@bco-dmo.org</gco:CharacterString>
				  </gmd:electronicMailAddress>
		    </gmd:CI_Address>
		  </gmd:address>
      <gmd:onlineResource>
          <gmd:CI_OnlineResource>
            <gmd:linkage>
              <gmd:URL>http://www.bco-dmo.org</gmd:URL>
            </gmd:linkage>
          </gmd:CI_OnlineResource>
        </gmd:onlineResource>
		  <gmd:hoursOfService>
        <gco:CharacterString>Monday - Friday 8:00am - 5:00pm</gco:CharacterString>
      </gmd:hoursOfService>
		  <gmd:contactInstructions>
		    <gco:CharacterString>For questions regarding this resource, please contact BCO-DMO via the email address provided.</gco:CharacterString>
		  </gmd:contactInstructions>
		</gmd:CI_Contact>
  </gmd:contactInfo>
  <gmd:role>
    <gmd:CI_RoleCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#CI_RoleCode" codeListValue="pointOfContact"  codeSpace="007">pointOfContact</gmd:CI_RoleCode>
  </gmd:role>
</gmd:CI_ResponsibleParty>
  </gmd:contact>
  <gmd:dateStamp>
    <gco:Date>2010-06-16</gco:Date>
  </gmd:dateStamp>
  <gmd:metadataStandardName>
    <gco:CharacterString>ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data</gco:CharacterString>
  </gmd:metadataStandardName>
  <gmd:metadataStandardVersion>
    <gco:CharacterString>ISO 19115-2:2009(E)</gco:CharacterString>
  </gmd:metadataStandardVersion>
  <gmd:referenceSystemInfo xlink:href="https://data.noaa.gov/docucomp/895cc120-95ed-11e0-aa80-0800200c9a66" xlink:title="WGS 84 / World Mercator"/>
  <gmd:identificationInfo>
    <gmd:MD_DataIdentification>
      <gmd:citation>
        <gmd:CI_Citation>
          <gmd:title>
            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset/2964.rdf" xlink:actuate="onRequest">Bacterial abundance and production from bottle samples from R/V Melville cruise COOK19MV from the Southern Ocean, south of New Zealand in 2002 (SOFeX project)</gmx:Anchor>
          </gmd:title>
          <gmd:date>
            <gmd:CI_Date>
              <gmd:date>
                <gco:Date>2008-08-15</gco:Date>
              </gmd:date>
              <gmd:dateType>
                <gmd:CI_DateTypeCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#CI_DateTypeCode" codeListValue="publication" codeSpace="ISOTC211/19115">publication</gmd:CI_DateTypeCode>
              </gmd:dateType>
            </gmd:CI_Date>
          </gmd:date>
          <gmd:date>
            <gmd:CI_Date>
              <gmd:date>
                <gco:Date>2008-08-15</gco:Date>
              </gmd:date>
              <gmd:dateType>
                <gmd:CI_DateTypeCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#CI_DateTypeCode" codeListValue="revision" codeSpace="ISOTC211/19115">revision</gmd:CI_DateTypeCode>
              </gmd:dateType>
            </gmd:CI_Date>
          </gmd:date>
          <gmd:edition>
            <gco:CharacterString/>
          </gmd:edition>
          <gmd:identifier>
            <gmd:MD_Identifier>
              <gmd:authority>
                <gmd:CI_Citation>
                  <gmd:title>
                    <gco:CharacterString>BCO-DMO Linked Data URI</gco:CharacterString>
                  </gmd:title>
                  <gmd:date>
                    <gmd:CI_Date>
                      <gmd:date>
                        <gco:Date>2008-08-15</gco:Date>
                      </gmd:date>
                      <gmd:dateType>
                        <gmd:CI_DateTypeCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#CI_DateTypeCode" codeListValue="creation">creation</gmd:CI_DateTypeCode>
                      </gmd:dateType>
                    </gmd:CI_Date>
                  </gmd:date>
                </gmd:CI_Citation>
              </gmd:authority>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset/2964" xlink:actuate="onRequest">http://lod.bco-dmo.org/id/dataset/2964</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmd:identifier>
          <gmd:citedResponsibleParty>
           <gmd:CI_ResponsibleParty>
              <gmd:individualName>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/person/50978.rdf" xlink:actuate="onRequest">Jacques  L. Oliver</gmx:Anchor>
              </gmd:individualName>
              <gmd:organisationName>
                <gmx:Anchor xlink:href="https://ror.org/04eeqc889" xlink:title="ROR ID" xlink:actuate="onRequest">Virginia Institute of Marine Science</gmx:Anchor>
              </gmd:organisationName>
              <gmd:positionName>
               <gco:CharacterString></gco:CharacterString>
              </gmd:positionName>
              <gmd:role>
                <gmd:CI_RoleCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#CI_RoleCode" codeListValue="principalInvestigator" codeSpace="008">principalInvestigator</gmd:CI_RoleCode>
              </gmd:role>
            </gmd:CI_ResponsibleParty>
          </gmd:citedResponsibleParty>
          <gmd:citedResponsibleParty>
            <gmd:CI_ResponsibleParty>
  <gmd:organisationName>
    <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/affiliation/191.rdf" xlink:actuate="onRequest">Biological and Chemical Oceanography Data Management Office (BCO-DMO)</gmx:Anchor>
  </gmd:organisationName>
  <gmd:contactInfo>
    <gmd:CI_Contact>
		  <gmd:phone>
		    <gmd:CI_Telephone>
				  <gmd:voice>
				    <gco:CharacterString>Unavailable</gco:CharacterString>
				  </gmd:voice>
				  <gmd:facsimile>
				    <gco:CharacterString>508-289-2009</gco:CharacterString>
				  </gmd:facsimile>
				</gmd:CI_Telephone>
		  </gmd:phone>
		  <gmd:address>
		    <gmd:CI_Address>
				  <gmd:deliveryPoint>
				    <gco:CharacterString>WHOI MS#36</gco:CharacterString>
				  </gmd:deliveryPoint>
				  <gmd:city>
				    <gco:CharacterString>Woods Hole</gco:CharacterString>
				  </gmd:city>
				  <gmd:administrativeArea>
				    <gco:CharacterString>MA</gco:CharacterString>
				  </gmd:administrativeArea>
				  <gmd:postalCode>
				    <gco:CharacterString>02543</gco:CharacterString>
				  </gmd:postalCode>
				  <gmd:country>
				    <gco:CharacterString>USA</gco:CharacterString>
				  </gmd:country>
				  <gmd:electronicMailAddress>
				    <gco:CharacterString>info@bco-dmo.org</gco:CharacterString>
				  </gmd:electronicMailAddress>
		    </gmd:CI_Address>
		  </gmd:address>
      <gmd:onlineResource>
          <gmd:CI_OnlineResource>
            <gmd:linkage>
              <gmd:URL>http://www.bco-dmo.org</gmd:URL>
            </gmd:linkage>
          </gmd:CI_OnlineResource>
        </gmd:onlineResource>
		  <gmd:hoursOfService>
        <gco:CharacterString>Monday - Friday 8:00am - 5:00pm</gco:CharacterString>
      </gmd:hoursOfService>
		  <gmd:contactInstructions>
		    <gco:CharacterString>For questions regarding this resource, please contact BCO-DMO via the email address provided.</gco:CharacterString>
		  </gmd:contactInstructions>
		</gmd:CI_Contact>
  </gmd:contactInfo>
  <gmd:role>
    <gmd:CI_RoleCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#CI_RoleCode" codeListValue="publisher" >publisher</gmd:CI_RoleCode>
  </gmd:role>
</gmd:CI_ResponsibleParty>
          </gmd:citedResponsibleParty>
          <gmd:otherCitationDetails>
            <gco:CharacterString>Cite this dataset as: Oliver, J. L. (2008) Bacterial abundance and production from bottle samples from R/V Melville cruise COOK19MV from the Southern Ocean, south of New Zealand in 2002 (SOFeX project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 15 August 2008) Version Date 2008-08-15 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/2964 [access date]</gco:CharacterString>
          </gmd:otherCitationDetails>
          </gmd:CI_Citation>
      </gmd:citation>
      <gmd:abstract>
        <gco:CharacterString>Bacterial Abundance and Production from bottle samples Methods and Sampling: &amp;lt;pre&amp;gt;
 &amp;lt;b&amp;gt;dates:&amp;lt;/b&amp;gt;           24 January 2002 to 21 February 2002  (20020124-20020221) 
 &amp;lt;b&amp;gt;location:&amp;lt;/b&amp;gt;        N: -52.56100  S: -66.61150  W: -172.69267  E: -166.94733 
&amp;lt;/pre&amp;gt;
&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;
&amp;lt;p class=&amp;quot;text&amp;quot;&amp;gt;
15 August 2008: Prepared for OCB data system by Cyndy Chandler, OCB DMO (WHOI) from documentation contributed by Jacques Olivier, Helen Quinby, Robert Daniels, and Hugh Ducklow&amp;lt;br&amp;gt;
&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Original Excel file contributed: 8 November, 2002 &amp;lt;br&amp;gt;
and downloaded from MBARI by OCB DMO:&amp;lt;/strong&amp;gt; 
  &amp;lt;a href=&amp;quot;http://ocb.whoi.edu/SOFeX/PI-NOTES/data_orig/SOFeX_melville_Bacterial_Data.xls&amp;quot; title=&amp;quot;orig file&amp;quot;&amp;gt;copy of original Excel file&amp;lt;/a&amp;gt;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Contact:&amp;lt;/strong&amp;gt; Jacques Oliver  (Email: jloliver@vims.edu)&amp;lt;/p&amp;gt;    

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;R/V Melville Bacterial Measurements Methodology&amp;lt;/strong&amp;gt;&amp;lt;br&amp;gt;
&amp;lt;br&amp;gt;

&amp;lt;strong&amp;gt;Bacterial Abundance   Flow cytometry (FCM) &amp;lt;/strong&amp;gt;&amp;lt;/p&amp;gt; 
&amp;lt;p&amp;gt; 1. 9.5 ml sample from CTD or TM rosette, fixed to final concentration of 1% formaldehyde &amp;lt;br&amp;gt; 
  2. Sample frozen at -80 C  &amp;lt;br&amp;gt; 
  3. On land, triplicate samples were analyzed using a Beckman-Coulter Epics Altra equipped with an Enterprise II laser at 488 nm using 190mV (intra-sample variation). &amp;lt;br&amp;gt; 
  4. About 100 ml of sample were measured following Troussellier et al. (1999) with addition of 2.5 mM SYTO 13 and a 10 minute incubation. &amp;lt;br&amp;gt; 
  5. This analysis was done on triplicate 1.0 ml samples to which 1.0 mm beads (Molecular Probes, Fluo Spheres, F-8888) were also added at a 10E6/ml concentration. &amp;lt;br&amp;gt; &amp;lt;br&amp;gt; 
  The beads determined flow rate and uniformity in fluorescence signal. Discrimination was done on a positive green fluorescence and the plot was green fluorescence (devoid of any red fluorescence) against side scatter. Analytical variation was 3%. 
&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Reference:&amp;lt;/strong&amp;gt;  Troussellier, M., C. Courties, P. Lebaron, and P.Servais. 1999. FEMS Microbiology Ecology 29: 319-330.&amp;lt;/p&amp;gt;      

&amp;lt;br&amp;gt;
&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Bacterial Abundance   Acridine orange direct counts (AODC)&amp;lt;/strong&amp;gt; 
&amp;lt;pre&amp;gt;  1. 10-15 ml sample fixed to final concentration of 1% formaldehyde               
  2. Sample filtered onto 0.2 micron blackened polycarbonate filter               
  3. Filter stained with 45% (w/v) acridine orange                
  4. Filter mounted onto glass slide with immersion oil and coverslip               
  5. Bacteria enumerated via epifluorescence microscopy               
  6. Intra-sample variation reported               
&amp;lt;/pre&amp;gt;
&amp;lt;br&amp;gt;
&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Reference:&amp;lt;/strong&amp;gt;  Kirchman, D., Sigda, J., Kapuscinski, R., and Mitchell, R. 1982. Appl. Envrion. Microbiol. 44:376-382 &amp;lt;/p&amp;gt;      

&amp;lt;br&amp;gt;
&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Bacterial Production   Thymidine (TdR) and Leucine (Leu) Incorporation&amp;lt;/strong&amp;gt; 

&amp;lt;pre&amp;gt;   1. 1.6 ml sample spiked with tritiated thymidine or tritiated leucine               
   2. Samples run in triplicate with one negative control containing 6% trichloroacetic acid (TCA)               
   3. Samples incubated at in situ temperatures in the dark for 8-20 hours (depending on water temp)               
   4. Incubation stopped by adding TCA (6% final conc.) to each sample               
   5. Nucleic acid extracted with TCA and ethanol               
   6. 1.6 ml Ultima Gold scintillation cocktail added               
   7. 3 minute counts on each sample on scintillation counter &amp;lt;/pre&amp;gt;
&amp;lt;br&amp;gt;
&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Reference:&amp;lt;/strong&amp;gt;  Smith, D.C. and Azam, F. 1992. Mar. Microb. Food Webs. 6:107-114.&amp;lt;/p&amp;gt;
              
&amp;lt;/pre&amp;gt;
&amp;lt;/p&amp;gt;</gco:CharacterString>
      </gmd:abstract>
      <gmd:status>
        <gmd:MD_ProgressCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#MD_ProgressCode" codeListValue="completed">completed</gmd:MD_ProgressCode>
      </gmd:status>
      <gmd:pointOfContact>
        <gmd:CI_ResponsibleParty>
          <gmd:individualName>
            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/person/50978.rdf" xlink:actuate="onRequest">Jacques  L. Oliver</gmx:Anchor>
          </gmd:individualName>
          <gmd:organisationName>
            <gmx:Anchor xlink:href="https://ror.org/04eeqc889" xlink:title="ROR ID" xlink:actuate="onRequest">Virginia Institute of Marine Science</gmx:Anchor>
          </gmd:organisationName>
          <gmd:contactInfo>
            <gmd:CI_Contact>
            <gmd:address>
                <gmd:CI_Address>
                                  <gmd:administrativeArea>
                    <gco:CharacterString>VA</gco:CharacterString>
                  </gmd:administrativeArea>                  <gmd:electronicMailAddress>
                    <gco:CharacterString>jloliver@vims.edu</gco:CharacterString>
                  </gmd:electronicMailAddress>
                </gmd:CI_Address>
              </gmd:address>
            </gmd:CI_Contact>
          </gmd:contactInfo>
          <gmd:role>
            <gmd:CI_RoleCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#CI_RoleCode" codeListValue="pointOfContact" codeSpace="007">pointOfContact</gmd:CI_RoleCode>
          </gmd:role>
        </gmd:CI_ResponsibleParty>
      </gmd:pointOfContact>
            <gmd:resourceMaintenance>
        <gmd:MD_MaintenanceInformation>
          <gmd:maintenanceAndUpdateFrequency>
            <gmd:MD_MaintenanceFrequencyCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#MD_MaintenanceFrequencyCode" codeListValue="asNeeded" codeSpace="009">asNeeded</gmd:MD_MaintenanceFrequencyCode>
          </gmd:maintenanceAndUpdateFrequency>
          <gmd:maintenanceNote>
            <gco:CharacterString>Dataset Version: 15 August 2008</gco:CharacterString>
          </gmd:maintenanceNote>
          </gmd:MD_MaintenanceInformation>
      </gmd:resourceMaintenance>
      <gmd:resourceFormat>
        <gmd:MD_Format>
          <gmd:name>
             <gco:CharacterString>Unknown</gco:CharacterString>
          </gmd:name>
          <gmd:version gco:nilReason="unknown"/>
          </gmd:MD_Format>
      </gmd:resourceFormat>
            <gmd:descriptiveKeywords>
        <gmd:MD_Keywords>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset-parameter/14965.rdf" xlink:title="http://lod.bco-dmo.org/id/parameter/829.rdf" xlink:actuate="onRequest">event</gmx:Anchor>
        </gmd:keyword>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset-parameter/14966.rdf" xlink:title="http://lod.bco-dmo.org/id/parameter/1073.rdf" xlink:actuate="onRequest">date_L</gmx:Anchor>
        </gmd:keyword>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset-parameter/14967.rdf" xlink:title="http://lod.bco-dmo.org/id/parameter/1073.rdf" xlink:actuate="onRequest">day_S</gmx:Anchor>
        </gmd:keyword>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset-parameter/14968.rdf" xlink:title="http://lod.bco-dmo.org/id/parameter/731.rdf" xlink:actuate="onRequest">lon</gmx:Anchor>
        </gmd:keyword>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset-parameter/14969.rdf" xlink:title="http://lod.bco-dmo.org/id/parameter/730.rdf" xlink:actuate="onRequest">lat</gmx:Anchor>
        </gmd:keyword>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset-parameter/14970.rdf" xlink:title="http://lod.bco-dmo.org/id/parameter/1073.rdf" xlink:actuate="onRequest">patch_loc</gmx:Anchor>
        </gmd:keyword>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset-parameter/14971.rdf" xlink:title="http://lod.bco-dmo.org/id/parameter/987.rdf" xlink:actuate="onRequest">station</gmx:Anchor>
        </gmd:keyword>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset-parameter/14972.rdf" xlink:title="http://lod.bco-dmo.org/id/parameter/1073.rdf" xlink:actuate="onRequest">ev_type</gmx:Anchor>
        </gmd:keyword>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset-parameter/14973.rdf" xlink:title="http://lod.bco-dmo.org/id/parameter/1107.rdf" xlink:actuate="onRequest">bot</gmx:Anchor>
        </gmd:keyword>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset-parameter/14974.rdf" xlink:title="http://lod.bco-dmo.org/id/parameter/808.rdf" xlink:actuate="onRequest">depth</gmx:Anchor>
        </gmd:keyword>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset-parameter/14975.rdf" xlink:title="http://lod.bco-dmo.org/id/parameter/1073.rdf" xlink:actuate="onRequest">light_lev</gmx:Anchor>
        </gmd:keyword>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset-parameter/14976.rdf" xlink:title="http://lod.bco-dmo.org/id/parameter/1073.rdf" xlink:actuate="onRequest">bact_cyt</gmx:Anchor>
        </gmd:keyword>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset-parameter/14977.rdf" xlink:title="http://lod.bco-dmo.org/id/parameter/1073.rdf" xlink:actuate="onRequest">bact_aodc</gmx:Anchor>
        </gmd:keyword>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset-parameter/14978.rdf" xlink:title="http://lod.bco-dmo.org/id/parameter/1605.rdf" xlink:actuate="onRequest">thy_incorp</gmx:Anchor>
        </gmd:keyword>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset-parameter/14979.rdf" xlink:title="http://lod.bco-dmo.org/id/parameter/1607.rdf" xlink:actuate="onRequest">thy_sd</gmx:Anchor>
        </gmd:keyword>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset-parameter/14980.rdf" xlink:title="http://lod.bco-dmo.org/id/parameter/1603.rdf" xlink:actuate="onRequest">leuc_incorp</gmx:Anchor>
        </gmd:keyword>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset-parameter/14981.rdf" xlink:title="http://lod.bco-dmo.org/id/parameter/1604.rdf" xlink:actuate="onRequest">leuc_sd</gmx:Anchor>
        </gmd:keyword>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset-instrument/4700.rdf" xlink:title="http://lod.bco-dmo.org/id/instrument/413.rdf" xlink:actuate="onRequest">Niskin Bottle</gmx:Anchor>
        </gmd:keyword>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset-instrument/4701.rdf" xlink:title="http://lod.bco-dmo.org/id/instrument/493.rdf" xlink:actuate="onRequest">Trace Metal Bottle</gmx:Anchor>
        </gmd:keyword>
        <gmd:type>
            <gmd:MD_KeywordTypeCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#MD_KeywordTypeCode" codeListValue="theme" codeSpace="005">theme</gmd:MD_KeywordTypeCode>
          </gmd:type>
          <gmd:thesaurusName>
            <gmd:CI_Citation>
              <gmd:title>
                <gco:CharacterString>None, User defined</gco:CharacterString>
              </gmd:title>
              <gmd:date gco:nilReason="unknown"/>
            </gmd:CI_Citation>
          </gmd:thesaurusName>
        </gmd:MD_Keywords>
      </gmd:descriptiveKeywords>
            <gmd:descriptiveKeywords>
        <gmd:MD_Keywords>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/parameter/829.rdf" xlink:title="Parameter" xlink:actuate="onRequest">event</gmx:Anchor>
        </gmd:keyword>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/parameter/1073.rdf" xlink:title="Parameter" xlink:actuate="onRequest">No BCO-DMO term</gmx:Anchor>
        </gmd:keyword>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/parameter/731.rdf" xlink:title="Parameter" xlink:actuate="onRequest">longitude</gmx:Anchor>
        </gmd:keyword>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/parameter/730.rdf" xlink:title="Parameter" xlink:actuate="onRequest">latitude</gmx:Anchor>
        </gmd:keyword>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/parameter/987.rdf" xlink:title="Parameter" xlink:actuate="onRequest">station</gmx:Anchor>
        </gmd:keyword>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/parameter/1107.rdf" xlink:title="Parameter" xlink:actuate="onRequest">bottle number</gmx:Anchor>
        </gmd:keyword>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/parameter/808.rdf" xlink:title="Parameter" xlink:actuate="onRequest">depth</gmx:Anchor>
        </gmd:keyword>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/parameter/1605.rdf" xlink:title="Parameter" xlink:actuate="onRequest">thymidine incorporation rate</gmx:Anchor>
        </gmd:keyword>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/parameter/1607.rdf" xlink:title="Parameter" xlink:actuate="onRequest">standard deviation of thymidine incorporation rate</gmx:Anchor>
        </gmd:keyword>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/parameter/1603.rdf" xlink:title="Parameter" xlink:actuate="onRequest">leucine incorporation rate</gmx:Anchor>
        </gmd:keyword>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/parameter/1604.rdf" xlink:title="Parameter" xlink:actuate="onRequest">standard deviation of leucine incorporation rate</gmx:Anchor>
        </gmd:keyword>
        <gmd:type>
            <gmd:MD_KeywordTypeCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#MD_KeywordTypeCode" codeListValue="featureType">featureType</gmd:MD_KeywordTypeCode>
          </gmd:type>
          <gmd:thesaurusName>
            <gmd:CI_Citation>
              <gmd:title>
                <gco:CharacterString>BCO-DMO Standard Parameters</gco:CharacterString>
              </gmd:title>
              <gmd:date gco:nilReason="unknown"/>
            </gmd:CI_Citation>
          </gmd:thesaurusName>
        </gmd:MD_Keywords>
      </gmd:descriptiveKeywords>
            <gmd:descriptiveKeywords>
        <gmd:MD_Keywords>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/413.rdf" xlink:title="Instrument" xlink:actuate="onRequest">Niskin bottle</gmx:Anchor>
        </gmd:keyword>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/493.rdf" xlink:title="Instrument" xlink:actuate="onRequest">Trace Metal Bottle</gmx:Anchor>
        </gmd:keyword>
        <gmd:type>
            <gmd:MD_KeywordTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MD_KeywordTypeCode" codeListValue="instrument">instrument</gmd:MD_KeywordTypeCode>
          </gmd:type>
          <gmd:thesaurusName>
            <gmd:CI_Citation>
              <gmd:title>
                <gco:CharacterString>BCO-DMO Standard Instruments</gco:CharacterString>
              </gmd:title>
              <gmd:date gco:nilReason="unknown"/>
            </gmd:CI_Citation>
          </gmd:thesaurusName>
        </gmd:MD_Keywords>
      </gmd:descriptiveKeywords>
            <gmd:descriptiveKeywords>
        <gmd:MD_Keywords>
                <gmd:keyword>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/deployment/57826.rdf" xlink:title="Cruise" xlink:actuate="onRequest">COOK19MV</gmx:Anchor>
        </gmd:keyword>
        <gmd:type>
            <gmd:MD_KeywordTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MD_KeywordTypeCode" codeListValue="service">service</gmd:MD_KeywordTypeCode>
          </gmd:type>
          <gmd:thesaurusName>
            <gmd:CI_Citation>
              <gmd:title>
                <gco:CharacterString>Deployment Activity</gco:CharacterString>
              </gmd:title>
              <gmd:date gco:nilReason="unknown"/>
            </gmd:CI_Citation>
          </gmd:thesaurusName>
        </gmd:MD_Keywords>
      </gmd:descriptiveKeywords>
            <gmd:descriptiveKeywords>
        <gmd:MD_Keywords>
        <gmd:keyword>
            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/deployment/57826.rdf" xlink:title="Deployment Location" xlink:actuate="onRequest">Southern Ocean</gmx:Anchor>
          </gmd:keyword>
          <gmd:type>
            <gmd:MD_KeywordTypeCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#MD_KeywordTypeCode" codeListValue="place" codeSpace="002">place</gmd:MD_KeywordTypeCode>
          </gmd:type>
          <gmd:thesaurusName>
            <gmd:CI_Citation>
              <gmd:title>
                <gco:CharacterString>Locations</gco:CharacterString>
              </gmd:title>
              <gmd:date gco:nilReason="unknown"/>
            </gmd:CI_Citation>
          </gmd:thesaurusName>
        </gmd:MD_Keywords>
      </gmd:descriptiveKeywords>
      <gmd:resourceConstraints>
        <gmd:MD_LegalConstraints>
          <gmd:accessConstraints>
            <gmd:MD_RestrictionCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#MD_RestrictionCode" codeListValue="otherRestrictions" codeSpace="008">otherRestrictions</gmd:MD_RestrictionCode>
          </gmd:accessConstraints>
          <gmd:useConstraints>
            <gmd:MD_RestrictionCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#MD_RestrictionCode" codeListValue="otherRestrictions" codeSpace="008">otherRestrictions</gmd:MD_RestrictionCode>
          </gmd:useConstraints>
          <gmd:otherConstraints>
            <gco:CharacterString>Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.</gco:CharacterString>
          </gmd:otherConstraints>
        </gmd:MD_LegalConstraints>
              </gmd:resourceConstraints>
      <gmd:aggregationInfo>
          <gmd:MD_AggregateInformation>
            <gmd:aggregateDataSetName>
              <gmd:CI_Citation>
                <gmd:title>
                  <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/program/2015.rdf" xlink:title="Program" xlink:actuate="onRequest">Ocean Carbon and Biogeochemistry</gmx:Anchor>
                </gmd:title>
                <gmd:date gco:nilReason="inapplicable"/>
                <gmd:citedResponsibleParty>
                  <gmd:CI_ResponsibleParty>
                    <gmd:contactInfo>
                      <gmd:CI_Contact>
                        <gmd:onlineResource>
                          <gmd:CI_OnlineResource>
                          <gmd:linkage>
                            <gmd:URL>http://us-ocb.org/</gmd:URL>
                          </gmd:linkage>
                          <gmd:name>
                            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/program/2015.rdf" xlink:title="Program Name" xlink:actuate="onRequest">Ocean Carbon and Biogeochemistry</gmx:Anchor>
                          </gmd:name>
                          <gmd:description>
                            <gco:CharacterString>The Ocean Carbon and Biogeochemistry (OCB) program focuses on the ocean's role as a component of the global Earth system, bringing together research in geochemistry, ocean physics, and ecology that inform on and advance our understanding of ocean biogeochemistry. The overall program goals are to promote, plan, and coordinate collaborative, multidisciplinary research opportunities within the U.S. research community and with international partners. Important OCB-related activities currently include: the Ocean Carbon and Climate Change (OCCC) and the North American Carbon Program (NACP); U.S. contributions to IMBER, SOLAS, CARBOOCEAN; and numerous U.S. single-investigator and medium-size research projects funded by U.S. federal agencies including NASA, NOAA, and NSF.
The scientific mission of OCB is to study the evolving role of the ocean in the global carbon cycle, in the face of environmental variability and change through studies of marine biogeochemical cycles and associated ecosystems.
The overarching OCB science themes include improved understanding and prediction of: 1) oceanic uptake and release of atmospheric CO2 and other greenhouse gases and 2) environmental sensitivities of biogeochemical cycles, marine ecosystems, and interactions between the two.
The OCB Research Priorities (updated January 2012) include: ocean acidification; terrestrial/coastal carbon fluxes and exchanges; climate sensitivities of and change in ecosystem structure and associated impacts on biogeochemical cycles; mesopelagic ecological and biogeochemical interactions; benthic-pelagic feedbacks on biogeochemical cycles; ocean carbon uptake and storage; and expanding low-oxygen conditions in the coastal and open oceans.</gco:CharacterString>
                          </gmd:description>
                          <gmd:function>
                            <gmd:CI_OnLineFunctionCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#CI_OnLineFunctionCode" codeListValue="information"/>
                          </gmd:function>
                      </gmd:CI_OnlineResource>
                    </gmd:onlineResource>
                  </gmd:CI_Contact>
                </gmd:contactInfo>
                <gmd:role/>
              </gmd:CI_ResponsibleParty>
            </gmd:citedResponsibleParty>
          </gmd:CI_Citation>
        </gmd:aggregateDataSetName>
          <gmd:aggregateDataSetIdentifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/program/2015.rdf" xlink:title="Program Acronym" xlink:actuate="onRequest">OCB</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmd:aggregateDataSetIdentifier>
          <gmd:associationType>
            <gmd:DS_AssociationTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#DS_AssociationTypeCode" codeListValue="largerWorkCitation">largerWorkCitation</gmd:DS_AssociationTypeCode>
          </gmd:associationType>
          <gmd:initiativeType>
            <gmd:DS_InitiativeTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#DS_InitiativeTypeCode" codeListValue="program">program</gmd:DS_InitiativeTypeCode>
          </gmd:initiativeType>
        </gmd:MD_AggregateInformation>
      </gmd:aggregationInfo><gmd:aggregationInfo>
          <gmd:MD_AggregateInformation>
            <gmd:aggregateDataSetName>
              <gmd:CI_Citation>
                <gmd:title>
                  <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/program/2017.rdf" xlink:title="Program" xlink:actuate="onRequest">Iron Synthesis</gmx:Anchor>
                </gmd:title>
                <gmd:date gco:nilReason="inapplicable"/>
                <gmd:citedResponsibleParty>
                  <gmd:CI_ResponsibleParty>
                    <gmd:contactInfo>
                      <gmd:CI_Contact>
                        <gmd:onlineResource>
                          <gmd:CI_OnlineResource>
                          <gmd:linkage>
                            <gmd:URL>https://osprey.bco-dmo.org/program/2017</gmd:URL>
                          </gmd:linkage>
                          <gmd:name>
                            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/program/2017.rdf" xlink:title="Program Name" xlink:actuate="onRequest">Iron Synthesis</gmx:Anchor>
                          </gmd:name>
                          <gmd:description>
                            <gco:CharacterString>The two main objectives of the Iron Synthesis program (SCOR Working Group proposal, 2005), are:
1. Data compilation: assembling a common open-access database of the in situ iron experiments, beginning with the first period (1993-2002; Ironex-1, Ironex-2, SOIREE, EisenEx, SEEDS-1; SOFeX, SERIES) where primary articles have already been published, to be followed by the 2004 experiments where primary articles are now in progress (EIFEX, SEEDS-2; SAGE, FeeP); similarly for the natural fertilizations S.O.JGOFS (1992), CROZEX (2004/2005) and KEOPS (2005).
2. Modeling and data synthesis of specific aspects of two or more such experiments for various topics such as physical mixing, phytoplankton productivity, overall ecosystem functioning, iron chemistry, CO2 budgeting, nutrient uptake ratios, DMS(P) processes, and combinations of these variables and processes.
SCOR Working Group proposal, 2005. &quot;The Legacy of in situ Iron Enrichments: Data Compilation and Modeling&quot;.
http://www.scor-int.org/Working_Groups/wg131.htm
See also: SCOR Proceedings Vol. 42 Concepcion, Chile October 2006, pgs: 13-16 2.3.3 Working Group on The Legacy of in situ Iron Enrichments: Data Compilation and Modeling.
The first objective of the Iron Synthesis program involves a data recovery effort aimed at assembling a common, open-access database of data and metadata from a series of in-situ ocean iron fertilization experiments conducted between 1993 and 2005. Initially, funding for this effort is being provided by the Scientific Committee on Oceanic Research (SCOR) and the U.S. National Science Foundation (NSF).
Through the combined efforts of the principal investigators of the individual projects and the staff of Biological and Chemical Oceanography Data Management Office (BCO-DMO), data currently available primarily through individuals, disparate reports and data agencies, and in multiple formats, are being collected and prepared for addition to the BCO-DMO database from which they will be freely available to the community.
As data are contributed to the BCO-DMO office, they are organized into four overlapping categories:
1. Level 1, basic metadata
(e.g., description of project/study, general location, PI(s), participants);
2. Level 2, detailed metadata and basic shipboard data and routine ship's operations
(e.g., CTDs, underway measurements, sampling event logs);
3. Level 3, detailed metadata and data from specialized observations
(e.g., discrete observations, experimental results, rate measurements) and
4. Level 4, remaining datasets
(e.g., highest level of detailed data available from each study).
Collaboration with BCO-DMO staff began in March of 2008 and initial efforts have been directed toward basic project descriptions, levels 1 and 2 metadata and basic data, with detailed and more detailed data files being incorporated as they become available and are processed.
Related file
Program Documentation
The Iron Synthesis Program is funded jointly by the Scientific Committee on Oceanic Research (SCOR) and the U.S. National Science Foundation (NSF).</gco:CharacterString>
                          </gmd:description>
                          <gmd:function>
                            <gmd:CI_OnLineFunctionCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#CI_OnLineFunctionCode" codeListValue="information"/>
                          </gmd:function>
                      </gmd:CI_OnlineResource>
                    </gmd:onlineResource>
                  </gmd:CI_Contact>
                </gmd:contactInfo>
                <gmd:role/>
              </gmd:CI_ResponsibleParty>
            </gmd:citedResponsibleParty>
          </gmd:CI_Citation>
        </gmd:aggregateDataSetName>
          <gmd:aggregateDataSetIdentifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/program/2017.rdf" xlink:title="Program Acronym" xlink:actuate="onRequest">FeSynth</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmd:aggregateDataSetIdentifier>
          <gmd:associationType>
            <gmd:DS_AssociationTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#DS_AssociationTypeCode" codeListValue="largerWorkCitation">largerWorkCitation</gmd:DS_AssociationTypeCode>
          </gmd:associationType>
          <gmd:initiativeType>
            <gmd:DS_InitiativeTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#DS_InitiativeTypeCode" codeListValue="program">program</gmd:DS_InitiativeTypeCode>
          </gmd:initiativeType>
        </gmd:MD_AggregateInformation>
      </gmd:aggregationInfo><gmd:aggregationInfo>
        <gmd:MD_AggregateInformation>
          <gmd:aggregateDataSetName>
              <gmd:CI_Citation>
                <gmd:title>
                  <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/project/2046.rdf" xlink:title="Project" xlink:actuate="onRequest">Southern Ocean Iron Experiment</gmx:Anchor>
                </gmd:title>
                <gmd:date gco:nilReason="inapplicable"/>
                <gmd:citedResponsibleParty>
                  <gmd:CI_ResponsibleParty>
                    <gmd:contactInfo>
                      <gmd:CI_Contact>
                        <gmd:onlineResource>
                          <gmd:CI_OnlineResource>
                          <gmd:linkage>
                            <gmd:URL>http://www.mbari.org/expeditions/SOFeX2002/</gmd:URL>
                          </gmd:linkage>
                          <gmd:name>
                            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/project/2046.rdf" xlink:title="Project Name" xlink:actuate="onRequest">Southern Ocean Iron Experiment</gmx:Anchor>
                          </gmd:name>
                          <gmd:description>
                            <gco:CharacterString>&lt;p&gt;Before he passed away in 1993, John Martin suggested that an increase in the flow of iron-rich dust to the ocean causes phytoplankton (single celled algae) to grow. The increased photosynthesis removes carbon dioxide from surface waters as the algae create biomass. This carbon dioxide is replaced by carbon dioxide gas that flows into the sea from the atmosphere. Reduced carbon dioxide in the atmosphere cools the planet (CO2 is a greenhouse gas that warms the earth). The results of this work, funded by the National Science Foundation, the Department of Energy, and the US Coast Guard, will be a much better understanding of how biological processes may regulate climate. (see Related Info: Fe cycle)&lt;/p&gt;
&lt;p&gt;A direct test of the 'Martin Hypothesis' that trace concentrations of Fe are responsible for phytoplankton's ability to grow by direct experimental addition of Fe to the surface waters. Consequently the distribution of bioavailable Fe in the surface waters determines large geographical areas primary production and the following flux of fixed organic matter to the deep sea. The aim of the SOFeX project is to investigate the effects of iron fertilization on the productivity of the Southern Ocean. The results of this work will contribute significantly to our understanding of important biogeochemical processes which bear directly on the global carbon cycle, atmospheric carbon dioxide concentration, and climate control.&lt;/p&gt;
&lt;p&gt;The SOFeX-N and SOFeX-S designations are sometimes used to distinguish between two iron enriched patches - one in low silicate waters north of the polar front (SOFEX-N), and the other in high silicate waters south of the polar front (SOFEX-S). All three ships, Melville (MV), Revelle (RR) and Polar Star (PS), worked in SOFEX-S, but only the Revelle and Melville worked in the SOFeX N patch and shuttled between the two patches.&lt;/p&gt;</gco:CharacterString>
                          </gmd:description>
                          <gmd:function>
                            <gmd:CI_OnLineFunctionCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#CI_OnLineFunctionCode" codeListValue="information"/>
                          </gmd:function>
                      </gmd:CI_OnlineResource>
                    </gmd:onlineResource>
                  </gmd:CI_Contact>
                </gmd:contactInfo>
                <gmd:role/>
              </gmd:CI_ResponsibleParty>
            </gmd:citedResponsibleParty>
          </gmd:CI_Citation>
        </gmd:aggregateDataSetName>
          <gmd:aggregateDataSetIdentifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/project/2046.rdf" xlink:title="Project Acronym" xlink:actuate="onRequest">SOFeX</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmd:aggregateDataSetIdentifier>
          <gmd:associationType>
            <gmd:DS_AssociationTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#DS_AssociationTypeCode" codeListValue="largerWorkCitation">largerWorkCitation</gmd:DS_AssociationTypeCode>
          </gmd:associationType>
          <gmd:initiativeType>
            <gmd:DS_InitiativeTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#DS_InitiativeTypeCode" codeListValue="project">project</gmd:DS_InitiativeTypeCode>
          </gmd:initiativeType>
        </gmd:MD_AggregateInformation>
      </gmd:aggregationInfo>
      <gmd:language>
        <gco:CharacterString>eng; USA</gco:CharacterString>
      </gmd:language>
      <gmd:topicCategory>
        <gmd:MD_TopicCategoryCode>oceans</gmd:MD_TopicCategoryCode>
      </gmd:topicCategory>
       <gmd:extent>
        <gmd:EX_Extent id="boundingExtent"><gmd:description>
            <gco:CharacterString>Southern Ocean</gco:CharacterString>
          </gmd:description><gmd:temporalElement>
            <gmd:EX_TemporalExtent>
              <gmd:extent>
                <gml:TimeInstant frame="#ISO-8601" gml:id="ID">
                  <gml:timePosition>2008-08-15</gml:timePosition>
                </gml:TimeInstant>
              </gmd:extent>
            </gmd:EX_TemporalExtent>
          </gmd:temporalElement>
          </gmd:EX_Extent>
      </gmd:extent>
      <gmd:extent>
          <gmd:EX_Extent>
            <gmd:geographicElement>
              <gmd:EX_GeographicDescription>
                <gmd:geographicIdentifier>
                  <gmd:MD_Identifier>
                    <gmd:code>
                      <gco:CharacterString>Southern Ocean, south of New Zealand</gco:CharacterString>
                    </gmd:code>
                  </gmd:MD_Identifier>
                </gmd:geographicIdentifier>
              </gmd:EX_GeographicDescription>
            </gmd:geographicElement>
          </gmd:EX_Extent>
        </gmd:extent></gmd:MD_DataIdentification>
  </gmd:identificationInfo>
  <gmd:contentInfo>
    <gmd:MD_FeatureCatalogueDescription>
      <gmd:includedWithDataset>
        <gco:Boolean>0</gco:Boolean>
      </gmd:includedWithDataset>
      <gmd:featureCatalogueCitation>
        <gmd:CI_Citation>
          <gmd:title>
            <gco:CharacterString>BCO-DMO catalogue of parameters from Bacterial abundance and production from bottle samples from R/V Melville cruise COOK19MV from the Southern Ocean, south of New Zealand in 2002 (SOFeX project)</gco:CharacterString>
          </gmd:title>
          <gmd:date gco:nilReason="unknown"/>
          <gmd:citedResponsibleParty>
            <gmd:CI_ResponsibleParty>
  <gmd:organisationName>
    <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/affiliation/191.rdf" xlink:actuate="onRequest">Biological and Chemical Oceanography Data Management Office (BCO-DMO)</gmx:Anchor>
  </gmd:organisationName>
  <gmd:contactInfo>
    <gmd:CI_Contact>
		  <gmd:phone>
		    <gmd:CI_Telephone>
				  <gmd:voice>
				    <gco:CharacterString>Unavailable</gco:CharacterString>
				  </gmd:voice>
				  <gmd:facsimile>
				    <gco:CharacterString>508-289-2009</gco:CharacterString>
				  </gmd:facsimile>
				</gmd:CI_Telephone>
		  </gmd:phone>
		  <gmd:address>
		    <gmd:CI_Address>
				  <gmd:deliveryPoint>
				    <gco:CharacterString>WHOI MS#36</gco:CharacterString>
				  </gmd:deliveryPoint>
				  <gmd:city>
				    <gco:CharacterString>Woods Hole</gco:CharacterString>
				  </gmd:city>
				  <gmd:administrativeArea>
				    <gco:CharacterString>MA</gco:CharacterString>
				  </gmd:administrativeArea>
				  <gmd:postalCode>
				    <gco:CharacterString>02543</gco:CharacterString>
				  </gmd:postalCode>
				  <gmd:country>
				    <gco:CharacterString>USA</gco:CharacterString>
				  </gmd:country>
				  <gmd:electronicMailAddress>
				    <gco:CharacterString>info@bco-dmo.org</gco:CharacterString>
				  </gmd:electronicMailAddress>
		    </gmd:CI_Address>
		  </gmd:address>
      <gmd:onlineResource>
          <gmd:CI_OnlineResource>
            <gmd:linkage>
              <gmd:URL>http://www.bco-dmo.org</gmd:URL>
            </gmd:linkage>
          </gmd:CI_OnlineResource>
        </gmd:onlineResource>
		  <gmd:hoursOfService>
        <gco:CharacterString>Monday - Friday 8:00am - 5:00pm</gco:CharacterString>
      </gmd:hoursOfService>
		  <gmd:contactInstructions>
		    <gco:CharacterString>For questions regarding this resource, please contact BCO-DMO via the email address provided.</gco:CharacterString>
		  </gmd:contactInstructions>
		</gmd:CI_Contact>
  </gmd:contactInfo>
  <gmd:role>
    <gmd:CI_RoleCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#CI_RoleCode" codeListValue="pointOfContact"  codeSpace="007">pointOfContact</gmd:CI_RoleCode>
  </gmd:role>
</gmd:CI_ResponsibleParty>
          </gmd:citedResponsibleParty>
          <gmd:otherCitationDetails>
            <gco:CharacterString>
            http://lod.bco-dmo.org/id/dataset-parameter/14965.rdf
	Name: event
	Units: doYhhmm
	Description: &lt;p&gt;unique sampling event number from event log (day of year and time (UTC))&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/14966.rdf
	Name: date_L
	Units: dimensionless
	Description: &lt;p&gt;NZ local day&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/14967.rdf
	Name: day_S
	Units: integer days
	Description: &lt;p&gt;South patch local date days since start of first fertilization, day zero=24-Jan ??&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/14968.rdf
	Name: lon
	Units: decimal degrees
	Description: &lt;p&gt;longitude; negative denotes West&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/14969.rdf
	Name: lat
	Units: decimal degrees
	Description: &lt;p&gt;latitude; negative denotes South&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/14970.rdf
	Name: patch_loc
	Units: dimensionless
	Description: &lt;p&gt;sampling location relative to patch see explanation in cruise event log), T = transect&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/14971.rdf
	Name: station
	Units: dimensionless
	Description: &lt;p&gt;station location number&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/14972.rdf
	Name: ev_type
	Units: dimensionless
	Description: &lt;p&gt;event type descriptor string&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/14973.rdf
	Name: bot
	Units: dimensionless
	Description: &lt;p&gt;Niskin bottle number&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/14974.rdf
	Name: depth
	Units: meters
	Description: &lt;p&gt;depth, calculted from pressure??&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/14975.rdf
	Name: light_lev
	Units: percent
	Description: &lt;p&gt;light level&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/14976.rdf
	Name: bact_cyt
	Units: cells/liter
	Description: &lt;p&gt;bacterial abundance, Flow Cytometry (FCM)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/14977.rdf
	Name: bact_aodc
	Units: cells/liter
	Description: &lt;p&gt;bacterial abundance, Acridine Orange Direct Count (AODC)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/14978.rdf
	Name: thy_incorp
	Units: picomoles/liter/hour
	Description: &lt;p&gt;Thymidine (TdR) incorporation rate&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/14979.rdf
	Name: thy_sd
	Units: picomoles/liter/hour
	Description: &lt;p&gt;standard deviation of thymidine incorporation rate&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/14980.rdf
	Name: leuc_incorp
	Units: picomoles/liter/hour
	Description: &lt;p&gt;Leucine incorporation rate&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/14981.rdf
	Name: leuc_sd
	Units: picomoles/liter/hour
	Description: &lt;p&gt;standard deviation of leucine incorporation rate&lt;/p&gt; 
</gco:CharacterString>
          </gmd:otherCitationDetails>
        </gmd:CI_Citation>
      </gmd:featureCatalogueCitation>
      <gmd:featureCatalogueCitation>
        <gmd:CI_Citation>
          <gmd:title>
            <gco:CharacterString>GB/NERC/BODC &gt; British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom</gco:CharacterString>
          </gmd:title>
          <gmd:date gco:nilReason="unknown"/>
          <gmd:citedResponsibleParty xlink:href="https://data.noaa.gov/docucomp/3D8FE2C9DA324D40939C8D007587E011" xlink:title="GB/NERC/BODC &gt; British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom"/>
          <gmd:otherCitationDetails>
            <gco:CharacterString>
            </gco:CharacterString>
          </gmd:otherCitationDetails>
        </gmd:CI_Citation>
      </gmd:featureCatalogueCitation>
    </gmd:MD_FeatureCatalogueDescription>
  </gmd:contentInfo>
  <gmd:distributionInfo>
    <gmd:MD_Distribution>
      <gmd:distributor>
        <gmd:MD_Distributor>
          <gmd:distributorContact>
            <gmd:CI_ResponsibleParty>
  <gmd:organisationName>
    <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/affiliation/191.rdf" xlink:actuate="onRequest">Biological and Chemical Oceanography Data Management Office (BCO-DMO)</gmx:Anchor>
  </gmd:organisationName>
  <gmd:contactInfo>
    <gmd:CI_Contact>
		  <gmd:phone>
		    <gmd:CI_Telephone>
				  <gmd:voice>
				    <gco:CharacterString>Unavailable</gco:CharacterString>
				  </gmd:voice>
				  <gmd:facsimile>
				    <gco:CharacterString>508-289-2009</gco:CharacterString>
				  </gmd:facsimile>
				</gmd:CI_Telephone>
		  </gmd:phone>
		  <gmd:address>
		    <gmd:CI_Address>
				  <gmd:deliveryPoint>
				    <gco:CharacterString>WHOI MS#36</gco:CharacterString>
				  </gmd:deliveryPoint>
				  <gmd:city>
				    <gco:CharacterString>Woods Hole</gco:CharacterString>
				  </gmd:city>
				  <gmd:administrativeArea>
				    <gco:CharacterString>MA</gco:CharacterString>
				  </gmd:administrativeArea>
				  <gmd:postalCode>
				    <gco:CharacterString>02543</gco:CharacterString>
				  </gmd:postalCode>
				  <gmd:country>
				    <gco:CharacterString>USA</gco:CharacterString>
				  </gmd:country>
				  <gmd:electronicMailAddress>
				    <gco:CharacterString>info@bco-dmo.org</gco:CharacterString>
				  </gmd:electronicMailAddress>
		    </gmd:CI_Address>
		  </gmd:address>
      <gmd:onlineResource>
          <gmd:CI_OnlineResource>
            <gmd:linkage>
              <gmd:URL>http://www.bco-dmo.org</gmd:URL>
            </gmd:linkage>
          </gmd:CI_OnlineResource>
        </gmd:onlineResource>
		  <gmd:hoursOfService>
        <gco:CharacterString>Monday - Friday 8:00am - 5:00pm</gco:CharacterString>
      </gmd:hoursOfService>
		  <gmd:contactInstructions>
		    <gco:CharacterString>For questions regarding this resource, please contact BCO-DMO via the email address provided.</gco:CharacterString>
		  </gmd:contactInstructions>
		</gmd:CI_Contact>
  </gmd:contactInfo>
  <gmd:role>
    <gmd:CI_RoleCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#CI_RoleCode" codeListValue="pointOfContact"  codeSpace="007">pointOfContact</gmd:CI_RoleCode>
  </gmd:role>
</gmd:CI_ResponsibleParty>
          </gmd:distributorContact>
        </gmd:MD_Distributor>
      </gmd:distributor>
      <gmd:transferOptions>
          <gmd:MD_DigitalTransferOptions>
          <gmd:transferSize>
              <gco:Real>15154</gco:Real>
            </gmd:transferSize><gmd:onLine>
            <gmd:CI_OnlineResource>
              <gmd:linkage><gmd:URL>https://datadocs.bco-dmo.org/file/B11vMZjsJ1NvA/bacteria.csv</gmd:URL></gmd:linkage>              <gmd:name>
                <gco:CharacterString>bacteria.csv</gco:CharacterString>
              </gmd:name><gmd:description>
                  <gco:CharacterString>Primary data file for dataset ID 2964</gco:CharacterString>
                </gmd:description><gmd:function>
                <gmd:CI_OnLineFunctionCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#CI_OnLineFunctionCode" codeListValue="download">download</gmd:CI_OnLineFunctionCode>
              </gmd:function>
            </gmd:CI_OnlineResource>
          </gmd:onLine>
        </gmd:MD_DigitalTransferOptions>
      </gmd:transferOptions>
              <gmd:transferOptions>
        <gmd:MD_DigitalTransferOptions>
        <gmd:onLine>
            <gmd:CI_OnlineResource>
              <gmd:linkage>
                <gmd:URL>https://osprey.bco-dmo.org/dataset/2964/data/download</gmd:URL>
              </gmd:linkage>
              <gmd:function>
                <gmd:CI_OnLineFunctionCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#CI_OnLineFunctionCode" codeListValue="download">download</gmd:CI_OnLineFunctionCode>
              </gmd:function>
            </gmd:CI_OnlineResource>
          </gmd:onLine>
        <gmd:offLine>
            <gmd:MD_Medium>
              <gmd:name>
                 <gmd:MD_MediumNameCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MD_MediumNameCode" codeListValue="onLine">onLine</gmd:MD_MediumNameCode>
              </gmd:name>
            </gmd:MD_Medium>
          </gmd:offLine>
        </gmd:MD_DigitalTransferOptions>
      </gmd:transferOptions>
    </gmd:MD_Distribution>
  </gmd:distributionInfo>
  <gmd:dataQualityInfo>
    <gmd:DQ_DataQuality>
      <gmd:scope>
        <gmd:DQ_Scope>
          <gmd:level>
            <gmd:MD_ScopeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MD_ScopeCode" codeListValue="dataset">dataset</gmd:MD_ScopeCode>
          </gmd:level>
        </gmd:DQ_Scope>
      </gmd:scope>
            <gmd:lineage>
        <gmd:LI_Lineage>
          <gmd:processStep xlink:title="Methods and Sampling">
            <gmd:LI_ProcessStep>
              <gmd:description>
                <gco:CharacterString>&amp;lt;pre&amp;gt;
 &amp;lt;b&amp;gt;dates:&amp;lt;/b&amp;gt;           24 January 2002 to 21 February 2002  (20020124-20020221) 
 &amp;lt;b&amp;gt;location:&amp;lt;/b&amp;gt;        N: -52.56100  S: -66.61150  W: -172.69267  E: -166.94733 
&amp;lt;/pre&amp;gt;
&amp;lt;br&amp;gt;&amp;lt;br&amp;gt;
&amp;lt;p class=&amp;quot;text&amp;quot;&amp;gt;
15 August 2008: Prepared for OCB data system by Cyndy Chandler, OCB DMO (WHOI) from documentation contributed by Jacques Olivier, Helen Quinby, Robert Daniels, and Hugh Ducklow&amp;lt;br&amp;gt;
&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Original Excel file contributed: 8 November, 2002 &amp;lt;br&amp;gt;
and downloaded from MBARI by OCB DMO:&amp;lt;/strong&amp;gt; 
  &amp;lt;a href=&amp;quot;http://ocb.whoi.edu/SOFeX/PI-NOTES/data_orig/SOFeX_melville_Bacterial_Data.xls&amp;quot; title=&amp;quot;orig file&amp;quot;&amp;gt;copy of original Excel file&amp;lt;/a&amp;gt;&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Contact:&amp;lt;/strong&amp;gt; Jacques Oliver  (Email: jloliver@vims.edu)&amp;lt;/p&amp;gt;    

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;R/V Melville Bacterial Measurements Methodology&amp;lt;/strong&amp;gt;&amp;lt;br&amp;gt;
&amp;lt;br&amp;gt;

&amp;lt;strong&amp;gt;Bacterial Abundance   Flow cytometry (FCM) &amp;lt;/strong&amp;gt;&amp;lt;/p&amp;gt; 
&amp;lt;p&amp;gt; 1. 9.5 ml sample from CTD or TM rosette, fixed to final concentration of 1% formaldehyde &amp;lt;br&amp;gt; 
  2. Sample frozen at -80 C  &amp;lt;br&amp;gt; 
  3. On land, triplicate samples were analyzed using a Beckman-Coulter Epics Altra equipped with an Enterprise II laser at 488 nm using 190mV (intra-sample variation). &amp;lt;br&amp;gt; 
  4. About 100 ml of sample were measured following Troussellier et al. (1999) with addition of 2.5 mM SYTO 13 and a 10 minute incubation. &amp;lt;br&amp;gt; 
  5. This analysis was done on triplicate 1.0 ml samples to which 1.0 mm beads (Molecular Probes, Fluo Spheres, F-8888) were also added at a 10E6/ml concentration. &amp;lt;br&amp;gt; &amp;lt;br&amp;gt; 
  The beads determined flow rate and uniformity in fluorescence signal. Discrimination was done on a positive green fluorescence and the plot was green fluorescence (devoid of any red fluorescence) against side scatter. Analytical variation was 3%. 
&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Reference:&amp;lt;/strong&amp;gt;  Troussellier, M., C. Courties, P. Lebaron, and P.Servais. 1999. FEMS Microbiology Ecology 29: 319-330.&amp;lt;/p&amp;gt;      

&amp;lt;br&amp;gt;
&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Bacterial Abundance   Acridine orange direct counts (AODC)&amp;lt;/strong&amp;gt; 
&amp;lt;pre&amp;gt;  1. 10-15 ml sample fixed to final concentration of 1% formaldehyde               
  2. Sample filtered onto 0.2 micron blackened polycarbonate filter               
  3. Filter stained with 45% (w/v) acridine orange                
  4. Filter mounted onto glass slide with immersion oil and coverslip               
  5. Bacteria enumerated via epifluorescence microscopy               
  6. Intra-sample variation reported               
&amp;lt;/pre&amp;gt;
&amp;lt;br&amp;gt;
&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Reference:&amp;lt;/strong&amp;gt;  Kirchman, D., Sigda, J., Kapuscinski, R., and Mitchell, R. 1982. Appl. Envrion. Microbiol. 44:376-382 &amp;lt;/p&amp;gt;      

&amp;lt;br&amp;gt;
&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Bacterial Production   Thymidine (TdR) and Leucine (Leu) Incorporation&amp;lt;/strong&amp;gt; 

&amp;lt;pre&amp;gt;   1. 1.6 ml sample spiked with tritiated thymidine or tritiated leucine               
   2. Samples run in triplicate with one negative control containing 6% trichloroacetic acid (TCA)               
   3. Samples incubated at in situ temperatures in the dark for 8-20 hours (depending on water temp)               
   4. Incubation stopped by adding TCA (6% final conc.) to each sample               
   5. Nucleic acid extracted with TCA and ethanol               
   6. 1.6 ml Ultima Gold scintillation cocktail added               
   7. 3 minute counts on each sample on scintillation counter &amp;lt;/pre&amp;gt;
&amp;lt;br&amp;gt;
&amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;Reference:&amp;lt;/strong&amp;gt;  Smith, D.C. and Azam, F. 1992. Mar. Microb. Food Webs. 6:107-114.&amp;lt;/p&amp;gt;
              
&amp;lt;/pre&amp;gt;
&amp;lt;/p&amp;gt;

from Cruise: COOK19MV &lt;pre&gt;
 &lt;b&gt;dates:&lt;/b&gt;           24 January 2002 to 21 February 2002  (20020124-20020221) 
 &lt;b&gt;location:&lt;/b&gt;        N: -52.56100  S: -66.61150  W: -172.69267  E: -166.94733 
&lt;/pre&gt;
&lt;br&gt;&lt;br&gt;
&lt;p class=&quot;text&quot;&gt;
15 August 2008: Prepared for OCB data system by Cyndy Chandler, OCB DMO (WHOI) from documentation contributed by Jacques Olivier, Helen Quinby, Robert Daniels, and Hugh Ducklow&lt;br&gt;
&lt;/p&gt;

&lt;p&gt;&lt;strong&gt;Original Excel file contributed: 8 November, 2002 &lt;br&gt;
and downloaded from MBARI by OCB DMO:&lt;/strong&gt; 
  &lt;a href=&quot;http://ocb.whoi.edu/SOFeX/PI-NOTES/data_orig/SOFeX_melville_Bacterial_Data.xls&quot; title=&quot;orig file&quot;&gt;copy of original Excel file&lt;/a&gt;&lt;/p&gt;

&lt;p&gt;&lt;strong&gt;Contact:&lt;/strong&gt; Jacques Oliver  (Email: jloliver@vims.edu)&lt;/p&gt;    

&lt;p&gt;&lt;strong&gt;R/V Melville Bacterial Measurements Methodology&lt;/strong&gt;&lt;br&gt;
&lt;br&gt;

&lt;strong&gt;Bacterial Abundance   Flow cytometry (FCM) &lt;/strong&gt;&lt;/p&gt; 
&lt;p&gt; 1. 9.5 ml sample from CTD or TM rosette, fixed to final concentration of 1% formaldehyde &lt;br&gt; 
  2. Sample frozen at -80 C  &lt;br&gt; 
  3. On land, triplicate samples were analyzed using a Beckman-Coulter Epics Altra equipped with an Enterprise II laser at 488 nm using 190mV (intra-sample variation). &lt;br&gt; 
  4. About 100 ml of sample were measured following Troussellier et al. (1999) with addition of 2.5 mM SYTO 13 and a 10 minute incubation. &lt;br&gt; 
  5. This analysis was done on triplicate 1.0 ml samples to which 1.0 mm beads (Molecular Probes, Fluo Spheres, F-8888) were also added at a 10E6/ml concentration. &lt;br&gt; &lt;br&gt; 
  The beads determined flow rate and uniformity in fluorescence signal. Discrimination was done on a positive green fluorescence and the plot was green fluorescence (devoid of any red fluorescence) against side scatter. Analytical variation was 3%. 
&lt;/p&gt;

&lt;p&gt;&lt;strong&gt;Reference:&lt;/strong&gt;  Troussellier, M., C. Courties, P. Lebaron, and P.Servais. 1999. FEMS Microbiology Ecology 29: 319-330.&lt;/p&gt;      

&lt;br&gt;
&lt;p&gt;&lt;strong&gt;Bacterial Abundance   Acridine orange direct counts (AODC)&lt;/strong&gt; 
&lt;pre&gt;  1. 10-15 ml sample fixed to final concentration of 1% formaldehyde               
  2. Sample filtered onto 0.2 micron blackened polycarbonate filter               
  3. Filter stained with 45% (w/v) acridine orange                
  4. Filter mounted onto glass slide with immersion oil and coverslip               
  5. Bacteria enumerated via epifluorescence microscopy               
  6. Intra-sample variation reported               
&lt;/pre&gt;
&lt;br&gt;
&lt;p&gt;&lt;strong&gt;Reference:&lt;/strong&gt;  Kirchman, D., Sigda, J., Kapuscinski, R., and Mitchell, R. 1982. Appl. Envrion. Microbiol. 44:376-382 &lt;/p&gt;      

&lt;br&gt;
&lt;p&gt;&lt;strong&gt;Bacterial Production   Thymidine (TdR) and Leucine (Leu) Incorporation&lt;/strong&gt; 

&lt;pre&gt;   1. 1.6 ml sample spiked with tritiated thymidine or tritiated leucine               
   2. Samples run in triplicate with one negative control containing 6% trichloroacetic acid (TCA)               
   3. Samples incubated at in situ temperatures in the dark for 8-20 hours (depending on water temp)               
   4. Incubation stopped by adding TCA (6% final conc.) to each sample               
   5. Nucleic acid extracted with TCA and ethanol               
   6. 1.6 ml Ultima Gold scintillation cocktail added               
   7. 3 minute counts on each sample on scintillation counter &lt;/pre&gt;
&lt;br&gt;
&lt;p&gt;&lt;strong&gt;Reference:&lt;/strong&gt;  Smith, D.C. and Azam, F. 1992. Mar. Microb. Food Webs. 6:107-114.&lt;/p&gt;
              
&lt;/pre&gt;
&lt;/p&gt;</gco:CharacterString>
              </gmd:description>
              <gmd:source>
                <gmd:LI_Source>
                  <gmd:sourceCitation>
                    <gmd:CI_Citation>
                      <gmd:title>
                        <gco:CharacterString>Specified by the Principal Investigator(s)</gco:CharacterString>
                      </gmd:title>
                      <gmd:date gco:nilReason="unknown"/>
                    </gmd:CI_Citation>
                  </gmd:sourceCitation>
                </gmd:LI_Source>
              </gmd:source>
            </gmd:LI_ProcessStep>
          </gmd:processStep>
          <gmd:processStep xlink:title="Data Processing Description">
            <gmd:LI_ProcessStep>
              <gmd:description>
                <gco:CharacterString>&amp;lt;pre&amp;gt;
 Change history:
    070509: downloaded original data (SOFeX_melville_Bacterial_Data.xls) from SOFeX project data web site.
            data prepared database by Dave DuBois (OCB DMO, WHOI) 
    080815: added to OCB database by Cyndy Chandler, OCB DMO, (cchandler@whoi.edu)
  
 OCB DMO Note: Four worksheets were contained in the original file: Methods, North Patch, 
    South Patch, and Transects. The Methods worksheet was extracted to file 
    Methods_Melville_Bact_Abund_Prod.xls. The other three worksheets were combined into one 
    and were sorted by date. The Latitude value for Transect 3, Station 42 was changed from 
    6 07.06 S to 66 07.06 S before converting all Lat and Lon values to decimal degrees.
    Two bottle cast entries were included for USCG Polar Star on 16-Feb in the original file 
    and are retained here with data collected during the Melville cruise.

 PI note: Date = Local Day (Kiwi). Event # = Julian Day, UTC. 
    North Patch Summary: 2 IN, 2 OUT. 
    South Patch Summary: 7 IN, 4 OUT (R/V Melville); 1 IN, 1 OUT (USCG Polar Star). 
    Transect Summary: 3 transects.

&amp;lt;/pre&amp;gt;

from Cruise: COOK19MV &lt;pre&gt;
 Change history:
    070509: downloaded original data (SOFeX_melville_Bacterial_Data.xls) from SOFeX project data web site.
            data prepared database by Dave DuBois (OCB DMO, WHOI) 
    080815: added to OCB database by Cyndy Chandler, OCB DMO, (cchandler@whoi.edu)
  
 OCB DMO Note: Four worksheets were contained in the original file: Methods, North Patch, 
    South Patch, and Transects. The Methods worksheet was extracted to file 
    Methods_Melville_Bact_Abund_Prod.xls. The other three worksheets were combined into one 
    and were sorted by date. The Latitude value for Transect 3, Station 42 was changed from 
    6 07.06 S to 66 07.06 S before converting all Lat and Lon values to decimal degrees.
    Two bottle cast entries were included for USCG Polar Star on 16-Feb in the original file 
    and are retained here with data collected during the Melville cruise.

 PI note: Date = Local Day (Kiwi). Event # = Julian Day, UTC. 
    North Patch Summary: 2 IN, 2 OUT. 
    South Patch Summary: 7 IN, 4 OUT (R/V Melville); 1 IN, 1 OUT (USCG Polar Star). 
    Transect Summary: 3 transects.

&lt;/pre&gt;</gco:CharacterString>
              </gmd:description>
              <gmd:source>
                <gmd:LI_Source>
                  <gmd:sourceCitation>
                    <gmd:CI_Citation>
                      <gmd:title>
                        <gco:CharacterString>Specified by the Principal Investigator(s)</gco:CharacterString>
                      </gmd:title>
                      <gmd:date gco:nilReason="unknown"/>
                    </gmd:CI_Citation>
                  </gmd:sourceCitation>
                </gmd:LI_Source>
              </gmd:source>
            </gmd:LI_ProcessStep>
          </gmd:processStep>
        </gmd:LI_Lineage>
      </gmd:lineage>
   </gmd:DQ_DataQuality>
  </gmd:dataQualityInfo>
  <gmd:metadataMaintenance>
    <gmd:MD_MaintenanceInformation>
      <gmd:maintenanceAndUpdateFrequency>
        <gmd:MD_MaintenanceFrequencyCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#MD_MaintenanceFrequencyCode" codeListValue="asNeeded" codeSpace="009">asNeeded</gmd:MD_MaintenanceFrequencyCode>
      </gmd:maintenanceAndUpdateFrequency>
      <gmd:maintenanceNote>
        <gco:CharacterString>7.x-1.1</gco:CharacterString>
      </gmd:maintenanceNote>
      <gmd:contact>
        <gmd:CI_ResponsibleParty>
  <gmd:organisationName>
    <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/affiliation/191.rdf" xlink:actuate="onRequest">Biological and Chemical Oceanography Data Management Office (BCO-DMO)</gmx:Anchor>
  </gmd:organisationName>
  <gmd:contactInfo>
    <gmd:CI_Contact>
		  <gmd:phone>
		    <gmd:CI_Telephone>
				  <gmd:voice>
				    <gco:CharacterString>Unavailable</gco:CharacterString>
				  </gmd:voice>
				  <gmd:facsimile>
				    <gco:CharacterString>508-289-2009</gco:CharacterString>
				  </gmd:facsimile>
				</gmd:CI_Telephone>
		  </gmd:phone>
		  <gmd:address>
		    <gmd:CI_Address>
				  <gmd:deliveryPoint>
				    <gco:CharacterString>WHOI MS#36</gco:CharacterString>
				  </gmd:deliveryPoint>
				  <gmd:city>
				    <gco:CharacterString>Woods Hole</gco:CharacterString>
				  </gmd:city>
				  <gmd:administrativeArea>
				    <gco:CharacterString>MA</gco:CharacterString>
				  </gmd:administrativeArea>
				  <gmd:postalCode>
				    <gco:CharacterString>02543</gco:CharacterString>
				  </gmd:postalCode>
				  <gmd:country>
				    <gco:CharacterString>USA</gco:CharacterString>
				  </gmd:country>
				  <gmd:electronicMailAddress>
				    <gco:CharacterString>info@bco-dmo.org</gco:CharacterString>
				  </gmd:electronicMailAddress>
		    </gmd:CI_Address>
		  </gmd:address>
      <gmd:onlineResource>
          <gmd:CI_OnlineResource>
            <gmd:linkage>
              <gmd:URL>http://www.bco-dmo.org</gmd:URL>
            </gmd:linkage>
          </gmd:CI_OnlineResource>
        </gmd:onlineResource>
		  <gmd:hoursOfService>
        <gco:CharacterString>Monday - Friday 8:00am - 5:00pm</gco:CharacterString>
      </gmd:hoursOfService>
		  <gmd:contactInstructions>
		    <gco:CharacterString>For questions regarding this resource, please contact BCO-DMO via the email address provided.</gco:CharacterString>
		  </gmd:contactInstructions>
		</gmd:CI_Contact>
  </gmd:contactInfo>
  <gmd:role>
    <gmd:CI_RoleCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#CI_RoleCode" codeListValue="pointOfContact"  codeSpace="007">pointOfContact</gmd:CI_RoleCode>
  </gmd:role>
</gmd:CI_ResponsibleParty>
      </gmd:contact>
    </gmd:MD_MaintenanceInformation>
  </gmd:metadataMaintenance>
  <gmi:acquisitionInformation>
    <gmi:MI_AcquisitionInformation>
    <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/413.rdf" xlink:title="Niskin bottle" xlink:actuate="onRequest">Niskin Bottle</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>Niskin Bottle</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Niskin Bottle Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle   Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:instrument>
        <gmi:MI_Instrument>
          <gmi:identifier>
            <gmd:MD_Identifier>
              <gmd:code>
                <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/instrument/493.rdf" xlink:title="Trace Metal Bottle" xlink:actuate="onRequest">Trace Metal Bottle</gmx:Anchor>
              </gmd:code>
            </gmd:MD_Identifier>
          </gmi:identifier>
          <gmi:type>
            <gco:CharacterString>Trace Metal Bottle</gco:CharacterString>
          </gmi:type>
          <gmi:description>
            <gco:CharacterString>PI Supplied Instrument Name: Trace Metal Bottle Instrument Name: Trace Metal Bottle Instrument Short Name:TM Bottle   Instrument Description: Trace metal (TM) clean rosette bottle used for collecting trace metal clean seawater samples. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/30/</gco:CharacterString>
          </gmi:description>
        </gmi:MI_Instrument>
      </gmi:instrument>
      <gmi:operation>
          <gmi:MI_Operation>
            <gmi:description>
              <gco:CharacterString>Cruise: COOK19MV</gco:CharacterString>
            </gmi:description>
            <gmi:identifier>
              <gmd:MD_Identifier>
                <gmd:code>
                  <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/deployment/57826.rdf" xlink:title="Cruise" xlink:actuate="onRequest">COOK19MV</gmx:Anchor>
                </gmd:code>
              </gmd:MD_Identifier>
            </gmi:identifier>
            <gmi:status>
              <gmd:MD_ProgressCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MD_ProgressCode" codeListValue="completed"/>
            </gmi:status>
            <gmi:type>
              <gmi:MI_OperationTypeCode codeList="https://data.noaa.gov/resources/iso19139/schema/resources/Codelist/gmxCodelists.xml#MI_OperationTypeCode" codeListValue="real"/>
            </gmi:type>
            <gmi:parentOperation gco:nilReason="inapplicable"/>
            <gmi:platform>
  <gmi:MI_Platform>
    <gmi:citation>
     <gmd:CI_Citation>
       <gmd:title>
         <gmx:Anchor xlink:href="http://shipsked.ucsd.edu/Ships/Melville/" xlink:actuate="onRequest">R/V Melville</gmx:Anchor>
       </gmd:title>
       <gmd:date gco:nilReason="unknown"/>
     </gmd:CI_Citation>
    </gmi:citation>
    <gmi:citation>
     <gmd:CI_Citation>
       <gmd:title>
         <gmx:Anchor xlink:href="http://vocab.nerc.ac.uk/collection/C17/current/318M" xlink:actuate="onRequest">Community Standard Description</gmx:Anchor>
       </gmd:title>
       <gmd:date gco:nilReason="unknown"/>
     </gmd:CI_Citation>
    </gmi:citation>
    <gmi:identifier>
      <gmd:MD_Identifier>
        <gmd:authority>
          <gmd:CI_Citation>
            <gmd:title>
              <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/authority/1.rdf" xlink:actuate="onRequest">International Council for the Exploration of the Sea</gmx:Anchor>
            </gmd:title>
            <gmd:date gco:nilReason="unknown"/>
          </gmd:CI_Citation>
        </gmd:authority>
        <gmd:code>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54057.rdf"
           xlink:title="318M" xlink:actuate="onRequest">R/V Melville</gmx:Anchor>
        </gmd:code>
      </gmd:MD_Identifier>
    </gmi:identifier>
    <gmi:description>
      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54057.rdf" xlink:title="R/V Melville" xlink:actuate="onRequest">vessel</gmx:Anchor>
    </gmi:description>
    <gmi:instrument gco:nilReason="unknown"/>
  </gmi:MI_Platform>
</gmi:platform>
            <gmi:plan>
              <gmi:MI_Plan>
                <gmi:status>
                  <gmd:MD_ProgressCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#MD_ProgressCode" codeListValue="completed"/>
                </gmi:status>
                <gmi:citation>
                  <gmd:CI_Citation>
                    <gmd:title>
                      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/deployment/57826.rdf" xlink:title="Cruise" xlink:actuate="onRequest">COOK19MV</gmx:Anchor>
                    </gmd:title>
                    <gmd:date gco:nilReason="unknown"/>
                    <gmd:citedResponsibleParty>
                      <gmd:CI_ResponsibleParty>
                      <gmd:individualName>
                          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/person/50576.rdf" xlink:actuate="onRequest">Kenneth H. Coale</gmx:Anchor>
                        </gmd:individualName>
                        <gmd:organisationName>
                          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/affiliation/79.rdf" xlink:title="Affiliation" xlink:actuate="onRequest">Moss Landing Marine Laboratories</gmx:Anchor>
                        </gmd:organisationName>
                        <gmd:contactInfo>
                        <gmd:CI_Contact>
                          <gmd:onlineResource>
                            <gmd:CI_OnlineResource>
                              <gmd:linkage>
                                <gmd:URL>http://ocb.whoi.edu/SOFeX/CRUISES/proj_description.pdf</gmd:URL>
                              </gmd:linkage>
                              <gmd:description>
                                <gco:CharacterString>Report describing COOK19MV</gco:CharacterString>
                              </gmd:description>
                            </gmd:CI_OnlineResource>
                          </gmd:onlineResource>
                        </gmd:CI_Contact>
                      </gmd:contactInfo>
                      <gmd:role>
                        <gmd:CI_RoleCode codeList="http://www.isotc211.org/2005/resources/Codelist/gmxCodelists.xml#CI_RoleCode" codeListValue="principalInvestigator"/>
                      </gmd:role>
                      </gmd:CI_ResponsibleParty>
                    </gmd:citedResponsibleParty>
                   </gmd:CI_Citation>
                </gmi:citation>
              </gmi:MI_Plan>
            </gmi:plan>
            </gmi:MI_Operation>
      </gmi:operation><gmi:platform>
  <gmi:MI_Platform>
    <gmi:citation>
     <gmd:CI_Citation>
       <gmd:title>
         <gmx:Anchor xlink:href="http://shipsked.ucsd.edu/Ships/Melville/" xlink:actuate="onRequest">R/V Melville</gmx:Anchor>
       </gmd:title>
       <gmd:date gco:nilReason="unknown"/>
     </gmd:CI_Citation>
    </gmi:citation>
    <gmi:citation>
     <gmd:CI_Citation>
       <gmd:title>
         <gmx:Anchor xlink:href="http://vocab.nerc.ac.uk/collection/C17/current/318M" xlink:actuate="onRequest">Community Standard Description</gmx:Anchor>
       </gmd:title>
       <gmd:date gco:nilReason="unknown"/>
     </gmd:CI_Citation>
    </gmi:citation>
    <gmi:identifier>
      <gmd:MD_Identifier>
        <gmd:authority>
          <gmd:CI_Citation>
            <gmd:title>
              <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/authority/1.rdf" xlink:actuate="onRequest">International Council for the Exploration of the Sea</gmx:Anchor>
            </gmd:title>
            <gmd:date gco:nilReason="unknown"/>
          </gmd:CI_Citation>
        </gmd:authority>
        <gmd:code>
          <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54057.rdf"
           xlink:title="318M" xlink:actuate="onRequest">R/V Melville</gmx:Anchor>
        </gmd:code>
      </gmd:MD_Identifier>
    </gmi:identifier>
    <gmi:description>
      <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/platform/54057.rdf" xlink:title="R/V Melville" xlink:actuate="onRequest">vessel</gmx:Anchor>
    </gmi:description>
    <gmi:instrument gco:nilReason="unknown"/>
  </gmi:MI_Platform>
</gmi:platform>
          </gmi:MI_AcquisitionInformation>
  </gmi:acquisitionInformation>
</gmi:MI_Metadata>
