http://lod.bco-dmo.org/id/dataset/3093
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Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2010-08-19
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Time-series Niskin-bottle sample data from R/V Hermano Gines cruises in the Cariaco Basin from 1995 through 2017 (CARIACO Ocean Time-Series Program)
2019-06-06
publication
2019-06-06
revision
Marine Biological Laboratory/Woods Hole Oceanographic Institution Library (MBLWHOI DLA)
2019-06-07
publication
https://doi.org/10.1575/1912/bco-dmo.3093.1
Frank Muller-Karger
University of South Florida
principalInvestigator
Yrene Astor
Estacion de Investigaciones Marinas de Margarita
principalInvestigator
Dr Mary I. Scranton
Stony Brook University - MSRC
principalInvestigator
Gordon T. Taylor
Stony Brook University - MSRC
principalInvestigator
Dr Robert C. Thunell
University of South Carolina
principalInvestigator
Ramon Varela
Estacion de Investigaciones Marinas de Margarita
principalInvestigator
Claudia R. Benitez-Nelson
University of South Carolina
principalInvestigator
Kristen Nicolle Buck
University of South Florida
principalInvestigator
Kent Fanning
University of South Florida
principalInvestigator
Juan Capelo
Estacion de Investigaciones Marinas de Margarita
principalInvestigator
Javier Gutierrez
Estacion de Investigaciones Marinas de Margarita
principalInvestigator
Laurencia Guzman
Estacion de Investigaciones Marinas de Margarita
principalInvestigator
Laura Lorenzoni
University of South Florida
principalInvestigator
Enrique Montes
University of South Florida
principalInvestigator
Jaimie Rojas
Estacion de Investigaciones Marinas de Margarita
principalInvestigator
Anadiuska Rondon
Estacion de Investigaciones Marinas de Margarita
principalInvestigator
Digna Rueda-Roa
University of South Florida
principalInvestigator
Eric Tappa
University of South Carolina
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Muller-Karger, F., Astor, Y., Scranton, M., Taylor, G., Thunell, R., Varela, R., Benitez-Nelson, C., Buck, K., Fanning, K., Capelo, J., Gutierrez, J., Guzman, L., Lorenzoni, L., Montes, E., Rojas, J., Rondon, A., Rueda-Roa, D., Tappa, E. (2019) Time-series Niskin-bottle sample data from R/V Hermano Gines cruises in the Cariaco Basin from 1995 through 2017 (CARIACO Ocean Time-Series Program). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 1) Version Date 2019-06-06 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.3093.1 [access date]
CARIACO time series Niskin bottle sample data Dataset Description: <p>The CARIACO Ocean Time-Series Program (formerly known as <u>CA</u>rbon <u>R</u>etention <u>I</u>n <u>A</u> <u>C</u>olored <u>O</u>cean) started on November 1995 (CAR-001) and ended on January 2017 (CAR-232). Monthly cruises were conducted to the CARIACO station (10.50° N, 64.67° W) onboard the R/V <em>Hermano Ginés</em> of the Fundación La Salle de Ciencias Naturales de Venezuela. The following sections describe the methods used in collecting the core observations at the CARIACO station.<br />
<br />
Methodology published at CARIACO site (http://imars.usf.edu/publications/methods-cariaco)<br />
CARIACO Field Program general description (http://www.imars.usf.edu/cariaco)<br />
<br />
Additional funding support provided by:<br />
Fondo Nacional de Ciencia, Tecnología e Investigación, FONACIT (2000001702 and 2011000353), Venezuela.<br />
Ley Orgánica de Ciencia, Tecnología e Innovación, LOCTI (Estación de Investigaciones Marinas, 23914), Venezuela.<br />
Inter-American Institute for Global Change Research, IAI (IAI-CRN3094).</p> Methods and Sampling: <div>Hydrocasts: CTD and Rosette Sample</div>
<div>During each cruise, a minimum of four hydrocasts were performed to collect a suite of core monthly observations. Additional hydrocasts were performed for specific process studies. We conducted separate shallow and deep casts to obtain better vertical resolution for chemical observations, and for productivity and pigment observations. Water was collected with a SeaBird rosette equipped with 12 (8 liter) teflon-coated Niskin bottles (bottle springs were also teflon-coated) at 20 depths between the surface and 1310 m. The rosette housed the CTD, which collected continuous profiles of temperature and salinity. The CTD also had a SBE-43 oxygen probe, a Wetlabs ECO fluorometer outfitted for chlorophyll-a estimates, and a C-Star transmissometer (660 nm, Wetlabs). Beam attenuation measurements were added to the time series on its 11th cruise (November 1986) originally using a SeaTech transmissometer. The rosette was controlled with a SeaBird deck unit via conducting cable, but alternatively it had been actuated automatically based on pressure recordings via an Autofire Module (SBE AFM) when breaks in cable conductivity had occurred.</div>
<div><br />
Between November 1995 and September 1996, three separate SBE-19 CTDs were used in repeated casts until a reliable salinity profile was obtained below the oxycline. The SBE-19 model CTDs frequently failed to provide reliable conductivity values below the oxycline in the Cariaco Basin. Starting in September 1996, the SBE-19 CTDs were replaced by SBE-25 CTDs, which provided extremely accurate and reliable data in anoxic waters.<br />
&nbsp;</div>
<div>All CTDs were calibrated at the Sea-Bird factory once per year. The accuracy of the pressure sensor was 3.5 m and had a resolution of 0.7 m. The temperatures accuracy was 0.002°C with a resolution of 0.0003°C. The conductivity accuracy was 0.003 mmho/cm with a resolution of 0.0004 mmho/cm.<br />
&nbsp;</div>
<div>Salinity</div>
<div>Continuous salinity profiles were calculated from the CTD measurements. Discrete salinity samples were analyzed using a Guildline Portasal 8410 salinometer standardized with IAPSO Standard Seawater, with a precision of better than ± 0.003 and a resolution of 0.0003 mS/cm at 15° C and 35 psu, the accuracy was ±0.003 at the same set point temperature as standardization and within -2° and +4°C of ambient. These salinity values were used to check, and when necessary calibrate, the CTD salinity profiles.<br />
&nbsp;</div>
<div>Discrete Oxygen</div>
<div>Continuous dissolved oxygen (O2) profiles were obtained with a SBE-43 Dissolved Oxygen Sensor coupled to the SBE-25 CTD. Discrete oxygen samples were collected in duplicate using glass-stoppered bottles and analyzed by Winkler titration (Strickland and Parsons, 1972, as modified by Aminot, 1983). The analytical precision for discrete oxygen analysis was ±3 mM, based on analysis of duplicate samples, with a detection limit of 5 mM.<br />
&nbsp;</div>
<div>Nutrients</div>
<div>Since CAR-072 (November 2001) all samples had been filtered through a 0.8 µm Nucleopore filter within minutes of collection, as recommended by the JGOFS protocol, and frozen in plastic bottles until analysis at the University of South Florida (USF). Previous to November 2001, nutrients were filtered through a 0.7 µm GF/F filter before freezing. This data was still considered reliable, as tests using glass fiber filters show no significant contamination. The analyses follow the standard techniques described by Strickland and Parsons (1972). USF follows the recommendations of Gordon et al. (1993) for the WOCE WHP project for nutrient analysis.<br />
&nbsp;</div>
<div>Since CAR-069 (August 2001) all silica samples were kept unfrozen; they were refrigerated and kept in the dark. Prior to CAR-069, silicates were frozen and those exhibiting high concentration of silica (&gt; 40µM below 300m in CARIACO) were affected by polymerization. All deep samples that were frozen showed low values due to polymerization loss, except CAR-063 and CAR-068 which showed high values. CAR-069 was analyzed by Yrene Astor at EDIMAR from the separate unfrozen bottlesand at USF from other, frozen, bottles. Unfrozen CAR-069 resulted higher with deep values close to what was expected (e.g. ∼92µM at 1310m).<br />
&nbsp;</div>
<div>Detection limits for CARIACO nutrient analysis</div>
<div>The limits below were determined by calculating the concentrations in triplicate standards, averaging the results within each triplicate group, calculating the standard deviation for each group, averaging the standard deviations, and finally doubling the averages to get the detection limits. These samples were analyzed on an ALPKEM RFA II. Subsequent Cariaco analyses were performed on a Technicon Analyzer II</div>
<div>
<table border="0" cellpadding="0">
<tbody>
<tr>
<td rowspan="2" style="width:165px">
<p><strong>Nutrient Type</strong></p>
</td>
<td style="width:136px">
<p><strong>ALPKEM RFA II</strong></p>
</td>
<td colspan="2" style="width:303px">
<p><strong>Technicon Analyzer II</strong></p>
</td>
</tr>
<tr>
<td style="width:136px">
<p><strong>Detection limits</strong></p>
</td>
<td style="width:142px">
<p><strong>Errors of analysis</strong></p>
</td>
<td style="width:159px">
<p><strong>Detection limits</strong></p>
</td>
</tr>
<tr>
<td style="width:165px">
<p><strong>PO<sub>4 </sub>Phosphate</strong></p>
</td>
<td style="width:136px">
<p>0.03 µmol</p>
</td>
<td style="width:142px">
<p>&lt;0.01 µM</p>
</td>
<td style="width:159px">
<p>0.02 µM</p>
</td>
</tr>
<tr>
<td style="width:165px">
<p><strong>Si(OH)<sub>4</sub> Silica</strong></p>
</td>
<td style="width:136px">
<p>0.14 µmol</p>
</td>
<td style="width:142px">
<p>0.2 µM</p>
</td>
<td style="width:159px">
<p>0.4 µM</p>
</td>
</tr>
<tr>
<td style="width:165px">
<p><strong>NO<sub>3</sub> Nitrate</strong></p>
</td>
<td style="width:136px">
<p>0.06 µmol</p>
</td>
<td style="width:142px">
<p>0.02 µM</p>
</td>
<td style="width:159px">
<p>0.04 µM</p>
</td>
</tr>
<tr>
<td style="width:165px">
<p><strong>NO<sub>2</sub> Nitrite</strong></p>
</td>
<td style="width:136px">
<p>0.02 µmol</p>
</td>
<td style="width:142px">
<p>&lt;0.01 µM</p>
</td>
<td style="width:159px">
<p>0.01 µM</p>
</td>
</tr>
<tr>
<td style="width:165px">
<p><strong>NH<sub>4</sub> Ammonium</strong></p>
</td>
<td style="width:136px">
<p>0.07 µmol</p>
</td>
<td style="width:142px">
<p>0.05 µM</p>
</td>
<td style="width:159px">
<p>0.1 µM</p>
</td>
</tr>
</tbody>
</table>
</div>
<div>&nbsp;</div>
<div>Primary Production</div>
<div>Primary productivity measurements were made using a modified Steeman Nielsen (1952) NaH14CO3 uptake assay. The productivity measurements consisted of in situ incubations of water collected at 8 depths and inoculated with 14C-labeled bicarbonate. One hour before sunrise, a shallow cast was performed to obtain water from 1, 7, 15, 25, 35, 55, 75, and 100 meters. As the productivity cast was taken, a Licor Photosynthetically-Active Radiation (PAR) integrator, placed high above the ship's bridge, was activated. Water was poured directly from the Niskin bottle under low light conditions into 250 ml clear polycarbonate bottles. These bottles had been previously acid-washed, rinsed, and soaked in de-ionized water for over 48 hours. Bottles were rinsed three times before filling, a near total fill (the volume within the bottles was actually 290 ml of sea water). Four clear polycarbonate bottles were filled from each depth. We wrap one inoculated bottle from each depth in aluminum foil to obtain the dark 14-C uptake rates. An extra bottle for 1, 15, 35, and 75 m was filled, but not inoculated, to provide time-zero (t0) filter and seawater blanks. The t0 samples were kept in the dark in the laboratory and were filtered after deploying the floating incubation buoy.<br />
&nbsp;</div>
<div>We inoculated each sample under low light conditions with 1,000 ml (4 mCi) of the 14C sodium bicarbonate working solution. A 200 ml aliquot for counting total added 14C activity was removed from one of the 3 bottles from each depth and placed in a 20 ml glass scintillation vial containing 250 ml ethanolamine. The mixture was held at 5°C until subsequent liquid scintillation analysis on shore. We also placed 50 ml of the 14C working solution in a vial with ethanolamine (250 ml) for reference counting.<br />
&nbsp;</div>
<div>The dark bottle and 3 light bottles were hooked together with a combination of plastic tie wraps and nylon cord, and kept in the dark while preparations were made for deployment of the productivity incubation float. At approximately 07:00 hours, the productivity array was deployed. The entire productivity ensemble was attached to a buoy equipped with a flag and radar reflector.<br />
&nbsp;</div>
<div>Productivity observations were initiated on December 1995. Between December 1995 and November 1996, we incubated samples from 06:00 to 10:00 hours. Starting December 1996, we changed our protocol to incubate between 07:00 and 11:00 hours. This more accurately represents 1/3 of the daily photoperiod and 1/3 of the total energy received in one day on a year-round basis at 10°30'N, as verified with the PAR light sensor.<br />
&nbsp;</div>
<div>Approximately 4 hours after deployment, the productivity array was recovered. We decided to use 4-hour incubation periods due to the potentially high productivity (&gt;1,000 mg/(m²d)) of this continental margin. Sample bottles were detached from the line and placed in labeled, dark plastic bags until filtration. Time and position of recovery were recorded. Maintaining low light conditions, a 50 ml aliquot was withdrawn from each productivity bottle using a 50 ml plastic syringe. This aliquot was filtered onto a 25 mm Whatman GF/F glass fiber filter, maintaining vacuum levels of ∼1/3 atm. The filter was rinsed with 0.25 ml 0.5 N HCl, and placed in a 20 ml glass scintillation vial, covered, and held at 5°C until subsequent processing on shore. At the shore laboratory, immediately upon return and within 15 hours of sample collection, 10 ml of liquid scintillation cocktail were added to the vials with the filters. These vials were refrigerated until they were ready for analysis on a BetaScout (PerkinElmer)scintillation counter.<br />
&nbsp;</div>
<div>Carbon uptake calculations followed the standard formulation outlined in the JGOFS manual (UNESCO, 1994), taking into consideration a (very low) quenching curve. Specifically, we subtracted the blank from all bottles, and then subtracted the dark bottle uptake from the average uptake in the light bottles to correct for non-photoautotrophic carbon fixation or absorption. Dark uptake values had always been very low. A scaling factor (∼3) was applied to convert the hourly production value to a "daily mean hourly average". This factor varies slightly, as it was based on the fraction of the energy received during the incubation period relative to the total energy received in a day. Daily rates were derived by multiplying the hourly rate by 12. Gieskes and Van Bennekom (1973), Peterson (1980), and Carpenter and Lively (1980) review the historical background, problems, and assumptions involved in the application of the radiocarbon technique to aquatic productivity. Muller-Karger (1984) also summarizes the technique and corrections involved.<br />
&nbsp;</div>
<div>pH and Alkalinity</div>
<div>pH samples were collected directly in 10-cm cells and analyzed on board. We measured pH and total Alkalinity estimates using the precise spectrophotometric dye methods developed by Robert-Baldo et al. (1985), Byrne and Breland (1989), and which we modified from Clayton and Byrne (1993) and Breland and Byrne (1993). These methods circumvent the problem that arises when potentiometric electrodes were transferred from dilute buffers to sea water samples due to the sample's high ionic strength. All the pH values were reported in the Master file for CARIACO data at 25 ℃ to avoid the effects of temperature on the solution chemistry. Measurement analytical precision for pHT at 25°C (total_hydrogen_ion_scale) = ±0.003, and for Total_alkalinity (mmol/kg), the precision is = 5 mmol/kg.&nbsp;<br />
&nbsp;</div>
<div>Corrections of pH for dye indicator impurities: The pH method uses the dye meta-cresol purple (mCP) as the pH indicator. The mCP dye used in CARIACO was in its unpurified form. Impurities in the indicator dye may cause uncertainty in measured pH values (Yao et al., 2007). Unpurified forms of the dye absorb significantly at the wavelength of maximum absorption for the acid species, HI- (434 nm) (Liu et al., 2011). The ratio of indicator absorbance at wavelengths 578 (base specie, I2-) and 434 (R = A578/A434) is used to calculate pH. Therefore, the effect of the impurities translates into apparent lower pH calculated values, especially at surface waters where pH &gt; 8.0 (Yao et al., 2007). The effect of the impurities varies from one indicator manufacturer to another, and from different batches of the same manufacturer (Yao et al., 2007). Fortunately, the indicator used for the whole dataset in CARIACO came from the same batch. Hence, a correction for mCP impurities was applied following the method developed by Douglas and Byrne (2017) to each set of data for each cruise. This correction translated to ~ -0.01 units at pH ~ 8.1, decreasing to ~ - 0.008 units at pH ~ 7.6. The corrections were applied to the whole dataset, and values for DIC and fCO2 were recalculated in the Master file. All the pH values were reported in the Master file for CARIACO data at 25 ℃ to avoid the effects of temperature on the solution chemistry.&nbsp;<br />
&nbsp;</div>
<div>Chlorophyll</div>
<div>Chlorophyll sample collection and storage: water samples were collected from Niskin bottles into 1 L dark polyethylene bottles. These samples were immediately filtered through 25 mm Whatman GF/F filters using a vacuum of less than 100 mm Hg. During the upwelling season (approx. January-May) we filtered 250 ml seawater, and during the rest of the year we filtered 500 ml. Three replicates were taken per depth during the upwelling season, but only two were collected when biomass was obviously at its minimum, during the non-upwelling season. Filters were folded in half twice and placed in glass centrifuge tubes, labeled and frozen. Storage time was kept as short as possible (less than a week) before measurement.<br />
&nbsp;</div>
<div>Chlorophyll procedure: after removal from the freezer, the filters were extracted in 10 ml of methanol. The samples were allowed to extract for 24 hours in the refrigerator. Following extraction, samples were centrifuged for 20 minutes to remove debris. The fluorometer (Turner fluorometer model 10-AU-005) was allowed to warm up and stabilize for 30 minutes prior to use. Pure methanol was measured to confirm the zero position. Samples were transferred to 1-cm cells and they were measured directly into the fluorometer (Fo). 100 µl of 0.48N HCl was added to each cell. A second reading was taken from the fluorometer for each cell (Fa). Standardization. The fluorometer was calibrated every year with a commercially available chlorophyll a standard (Σ). The concentration of chlorophyll-an and phaeopigments in the sample were calculated using Yentsh and Menzel (1963) equation, with a specific absorption coefficient of 74.5 (chlorophyll in methanol).<br />
&nbsp;</div>
<div>HPLC</div>
<div>HPLC analysis was restarted in July 2006 (CAR-123). Samples were filtered 47 mm Whatman GF/F filters at 8 depths (1, 7, 15, 25, 35, 55, 75 and 100m). The volume filtered depends on the amount of particles in the water. Replicates were taken at the 1m depth. Filters were stored in aluminum envelopes and stored in the fridge until reaching shore. Once on shore, samples were stored at -40°C until transportation to the US. Horn Point Laboratory (http://www.hpl.umces.edu/) performs the analyses through a collaborative agreement with NASA. The method used was Van Heukelem and Thomas (2001).&nbsp;<br />
&nbsp;</div>
<div>POC and PON</div>
<div>POC and PON sample collection and storage: water samples were collected from Niskin bottles into 2 L dark polyethylene bottles. These samples were immediately filtered through 25 mm Whatman GF/F filters (precombusted for 5 hours at 450°C) using a vacuum of less than 100 mm Hg. Since July 2007 (CAR-135) filters were acidified (10% HCl) after combustion and prior to sample collection. A portion of these filters was used for POP analysis (see below). Filters were placed on expendable tin disks and then into aluminum foil envelops (also precombusted for 5 hours at 450°C) labeled and frozen. In the laboratory, filters were dried at 65°C for 12-15 hours then stored with silica gel.</div>
<div>Measurement: The filters were folded inside a tin disk and analyzed on a Perkin Elmer 2400 Elemental Analyzer. The samples were combusted at 1200-1300°C and then passed through a reduction tube to removes the oxygen added to raise the combustion temperature. Filers were not acid fumed prior to analysis. The C and N were then separated in a chromatographic column and were measured on a Thermal Conductivity Detector. Carbon and nitrogen standards, and blank filters were used to calibrate the data. The accuracy of the instrument was &lt;0.3% and the precision of the instrument was &lt;0.2%. These were published values and we find that we were always within these limits (usually ±0.15% for carbon and ±0.1% for nitrogen). We ran cystine as our standard (29.99% Carbon, 11.66% Nitrogen). The analytical range of the instrument is: Carbon= .001 to 3.6 mg and Nitrogen= 0.001 to 6.0 mg.<br />
&nbsp;</div>
<div>POP</div>
<div>POP was analyzed from the same POC/PON filters. The method used for the SRP analysis was based on Koroleff (1983).<br />
&nbsp;</div>
<div>Dissolved organic Carbon, Nitrogen and Phosphorous (DOC, DON and DOP)</div>
<div>Measurements of DOC were taken since the beginning of the project in November 1995 but suspended in February 2001 (CAR-062) due to irregularity of results. DOC was reinitiated in March 2005 (CAR-110) using a new protocol. DOC samples were collected monthly and analyzed at the Organic Biogeochemistry Lab in the Rosenstiel School of Marine &amp; Atmospheric Science at the University of Miami. Samples were gravity-filtered directly from the Niskin bottles through 45 mm GF/F precombusted filters using acid cleaned polycarbonate in-line filter holder. Immediately after filtration the polyethylene bottles were frozen at -20°C until analysis.<br />
&nbsp;</div>
<div>DON and DOP measurements were added to the regular CARIACO cruises in July 2004 (CAR-101). Samples were filtered through GF/F filters using a specially built vacuum filter rack. The DON method was based on Solorzano and Sharp (1980). This procedure produces a filtered seawater sample for analysis of total dissolved fixed nitrogen (=nitrate + nitrite + ammonium + DON). DON concentration was obtained by difference from nitrate, nitrite, and ammonium measured in the standard nutrient protocol. DOP was analyzed in the same persulfate-oxidized filtrate solution as DON. That solution yields total inorganic phosphate concentration, which was composed of the inorganic phosphate concentration originally in the seawater, plus an additional phosphate concentration due to the conversion of DOP to phosphate. DOP concentration was then obtained by difference from the inorganic phosphate in the unoxidized sample measured through the standard nutrient protocol.</div>
<div>&nbsp;</div>
<div>Optical measurements</div>
<div>In-water measurements: a PRR-600 (Biospherical) was used to retrieve downwelling irradiance and upwelling radiance. From these, PAR, Kd and reflectance can be calculated. Beam attenuation coefficient (Cp) was measured using a C-Star transmissometer (see section Hydrocasts: CTD and Rosette Sample), which provides measurements at 660 nm throughout the entire water column.<br />
&nbsp;</div>
<div>CDOM samples were measured at four depths (1m, 15m, 25m, 50m) by filtration through a 0.2 µm pore size filter and immediately frozen at -20°C. Before being analyzed, they were thawed and re-filtered to eliminate any salt crystals that may had formed. CDOM was measured between 200 and 800 nm, with a 0.3 nm interval, using a dual fiber optic spectrometer (Ocean Optics) equipped with 10-cm quartz cuvettes and distilled water as a blank.<br />
&nbsp;</div>
<div>Above water measurements: a PR-650 (Photoresearch) measures sky radiance (Ls), water leaving radiance (Lw) and total irradiance (Es) at an angle of 30°. From these measurements, remote sensing reflectance (Rrs = Lw/Es) can be calculated and used in satellite sensor (such as MODIS and SeaWiFS) calibration.<br />
&nbsp;</div>
<div>Methods compiled by John Akl, July 2002. Revised November 2005 by Laura Lorenzoni. Revised April 2019 by Digna Rueda-Roa<br />
&nbsp;</div>
<div>The CARIACO Ocean Time-Series Program (November 1995 – January 2017)</div>
<p>For a detailed log for each cruise, please refer to the supplemental document Cruise Data Acquisition Report (https://datadocs.bco-dmo.org/docs/302/CARIACO/data_docs/3092/1/Cruise_data_aquisition_report.xlsx)</p>
Funding provided by Fondo Nacional de Ciencia, Tecnología e Innovación of Venezuela (FONACIT) Award Number: unknown CARIACO FONACIT
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-9401537 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=9401537
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-9729697 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=9729697
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0326268 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0326268
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-9216626 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=9216626
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-9711318 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=9711318
Funding provided by National Aeronautics & Space Administration (NASA) Award Number: NAS5-97128
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-9415790 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=9415790
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-9729284 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=9729284
Funding provided by National Aeronautics & Space Administration (NASA) Award Number: NAG5-6448
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0963028 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0963028
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0752139 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0752139
Funding provided by Fondo Nacional de Ciencia, Tecnología e Innovación of Venezuela (FONACIT) Award Number: 96280221
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: unknown CARIACO NSF OCE
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0326313 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0326313
Funding provided by National Aeronautics & Space Administration (NASA) Award Number: NNX14AP62A Award URL: https://federalreporter.nih.gov/Projects/Details/?projectId=674021
completed
Frank Muller-Karger
University of South Florida
(727) 553-3335
College of Marine Science 140 7th Avenue South
St Petersburg
FL
33701
USA
carib@marine.usf.edu
pointOfContact
Yrene Astor
Estacion de Investigaciones Marinas de Margarita
Venezuela
yastor@edimar.org
pointOfContact
Dr Mary I. Scranton
Stony Brook University - MSRC
631-632-8735
Stony Brook University
Stony Brook
NY
11794-5000
USA
mscranton@notes.cc.sunysb.edu
pointOfContact
Gordon T. Taylor
Stony Brook University - MSRC
631-632-8688
Stony Brook University School of Marine and Atmospheric Sciences
Stony Brook
NY
11794-5000
USA
gordon.taylor@stonybrook.edu
pointOfContact
Dr Robert C. Thunell
University of South Carolina
803-777-7593
Department of Geological Sciences, University of South Carolina 700 Sumter Street
Columbia
SC
29208
USA
thunell@geol.sc.edu
pointOfContact
Ramon Varela
Estacion de Investigaciones Marinas de Margarita
Venezuela
rvarela@edimar.org
pointOfContact
Claudia R. Benitez-Nelson
University of South Carolina
803-777-0525
School of the Earth, Ocean and Environment 701 Sumter Street
Columbia
SC
29208
USA
benitezn@mailbox.sc.edu
pointOfContact
Kristen Nicolle Buck
University of South Florida
541-737-7401
2651 SW Orchard Avenue CEOAS
Corvallis
OR
97331
USA
kristen.buck@oregonstate.edu
pointOfContact
Kent Fanning
University of South Florida
727 553 1594
140 Seventh Avenue, South
St. Petersburg
FL
33701
USA
kaf@seas.marine.usf.edu
pointOfContact
Juan Capelo
Estacion de Investigaciones Marinas de Margarita
pointOfContact
Javier Gutierrez
Estacion de Investigaciones Marinas de Margarita
Venezuela
pointOfContact
Laurencia Guzman
Estacion de Investigaciones Marinas de Margarita
pointOfContact
Laura Lorenzoni
University of South Florida
727-553-3987
University of South Florida 140th 7th Av. South
St. Petersburg
FL
33701
USA
laural@marine.usf.edu
pointOfContact
Enrique Montes
University of South Florida
pointOfContact
Jaimie Rojas
Estacion de Investigaciones Marinas de Margarita
pointOfContact
Anadiuska Rondon
Estacion de Investigaciones Marinas de Margarita
pointOfContact
Digna Rueda-Roa
University of South Florida
University of South Florida College of Marine Science
St. Petersburg
FL
pointOfContact
Eric Tappa
University of South Carolina
tappa@geol.sc.edu
pointOfContact
asNeeded
Dataset Version: 1
Unknown
Cruise_number
Cruise_ID_1
Cruise_ID_2
Leg
Day
Month
Year
Hydro_cast_no
ISO_DateTime_start_hc_local
ISO_DateTime_end_hc_local
Depth_target
Depth_real
O2_ml_L
q_O2_ml_L
O2_umol_kg
q_O2_umol_kg
NO3_UDO
q_NO3_UDO
PO4_UDO
q_PO4_UDO
SiO4_UDO
q_SiO4_UDO
NH4_USF
q_NH4_USF
NO2_USF
q_NO2_USF
NO3_NO2_USF
q_NO3_NO3_USF
PO4_USF
q_PO4_USF
SiO4_USF
q_SiO4_USF
pH
q_pH
Alkalinity_mol_kg
q_Alkalinity_mol_kg
Alkalinity_umol_kg
q_Alkalinity_umol_kg
TCO2
q_TCO2
fCO2
q_fCO2
pH_corrected
q_pH_corrected
TCO2_corrected
q_TCO2_corrected
fCO2_corrected
q_fCO2_corrected
Salinity_bottles
q_Salinity_bottles
Salinity_CTD
q_Salinity_CTD
Temperature
q_Temperature
Sigma_t
q_Sigma_t
TPP
q_TPP
PIP
q_PIP
POC_ug_kg
q_POC_ug_kg
PON_ug_kg
q_PON_ug_kg
POC_ug_L
q_POC_ug_L
PN_ug_L
q_PN_ug_L
C_N_particulate
q_C_N_particulate
DON
q_DON
DOP
q_DOP
DOC
q_DOC
TOC
q_TOC
PrimaryProductivity
q_PrimaryProductivity
Chlorophyll
q_Chlorophyll
Phaeopigments
q_Phaeopigments
Total_Prokaryotes
Bact_Biomass_mgC_m3
Bact_Biomass_uMC
Bio_cast_no
ISO_DateTime_start_bc_local
ISO_DateTime_end_bc_local
ISO_DateTime_start_hc_UTC
ISO_DateTime_end_hc_UTC
ISO_DateTime_start_bc_UTC
ISO_DateTime_end_bc_UTC
Latitude
Longitude
ALPKEM RFA II
SBE-19
Perkin Elmer 2400 Elemental Analyzer.
HPLC
Licor Photosynthetically-Active Radiation (PAR) integrator
BetaScout (PerkinElmer) scintillation counter
Niskin Bottle
SeaTech transmissometer
SBE-25
SBE-43 oxygen probe
Technicon Analyzer II
Turner fluorometer model 10-AU-005
C-Star transmissometer
Wetlabs ECO fluorometer
theme
None, User defined
No BCO-DMO term
leg of cruise
day of month
month of year
year
cast
ISO_DateTime_Local
depth nominal
depth
dissolved Oxygen
quality flag
dissolved Oxygen
Nitrate
reactive phosphorus (PO4)
Silicate, Si(OH)4, silicic acid
Ammonium
Nitrite
nitrate plus nitrite
pH seawater scale
total alkalinity (TA)
total Carbon Dioxide in seawater
fugacity of CO2
salinity from water bottle samples
salinity from CTD
water temperature at measurement depth
sigma-t
particulate organic Carbon (POC)
particulate organic nitrogen
Carbon to Nitrogen ratio (C:N)
Dissolved Organic Nitrogen
Dissolved Organic Phosphorus
dissolved organic Carbon
total organic Carbon
chlorophyll a
total phaeopigment
ISO_DateTime_UTC
latitude
longitude
featureType
BCO-DMO Standard Parameters
Alpkem RFA300
CTD Sea-Bird SEACAT 19
Elemental Analyzer
High-Performance Liquid Chromatograph
LI-COR Biospherical PAR Sensor
Liquid Scintillation Counter
Niskin bottle
Sea Tech Transmissometer
Sea-Bird SBE 25 Sealogger CTD
Sea-Bird SBE 43 Dissolved Oxygen Sensor
Technicon AutoAnalyzer II
Turner Designs Fluorometer 10-AU
WET Labs {Sea-Bird WETLabs} C-Star transmissometer
WETLabs ECO-FLNTU
instrument
BCO-DMO Standard Instruments
HG93_CARIACO
service
Deployment Activity
CARIACO basin
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Ocean Carbon and Biogeochemistry
http://us-ocb.org/
Ocean Carbon and Biogeochemistry
The Ocean Carbon and Biogeochemistry (OCB) program focuses on the ocean's role as a component of the global Earth system, bringing together research in geochemistry, ocean physics, and ecology that inform on and advance our understanding of ocean biogeochemistry. The overall program goals are to promote, plan, and coordinate collaborative, multidisciplinary research opportunities within the U.S. research community and with international partners. Important OCB-related activities currently include: the Ocean Carbon and Climate Change (OCCC) and the North American Carbon Program (NACP); U.S. contributions to IMBER, SOLAS, CARBOOCEAN; and numerous U.S. single-investigator and medium-size research projects funded by U.S. federal agencies including NASA, NOAA, and NSF.
The scientific mission of OCB is to study the evolving role of the ocean in the global carbon cycle, in the face of environmental variability and change through studies of marine biogeochemical cycles and associated ecosystems.
The overarching OCB science themes include improved understanding and prediction of: 1) oceanic uptake and release of atmospheric CO2 and other greenhouse gases and 2) environmental sensitivities of biogeochemical cycles, marine ecosystems, and interactions between the two.
The OCB Research Priorities (updated January 2012) include: ocean acidification; terrestrial/coastal carbon fluxes and exchanges; climate sensitivities of and change in ecosystem structure and associated impacts on biogeochemical cycles; mesopelagic ecological and biogeochemical interactions; benthic-pelagic feedbacks on biogeochemical cycles; ocean carbon uptake and storage; and expanding low-oxygen conditions in the coastal and open oceans.
OCB
largerWorkCitation
program
U.S. Joint Global Ocean Flux Study
http://usjgofs.whoi.edu/
U.S. Joint Global Ocean Flux Study
The United States Joint Global Ocean Flux Study was a national component of international JGOFS and an integral part of global climate change research.
The U.S. launched the Joint Global Ocean Flux Study (JGOFS) in the late 1980s to study the ocean carbon cycle. An ambitious goal was set to understand the controls on the concentrations and fluxes of carbon and associated nutrients in the ocean. A new field of ocean biogeochemistry emerged with an emphasis on quality measurements of carbon system parameters and interdisciplinary field studies of the biological, chemical and physical process which control the ocean carbon cycle. As we studied ocean biogeochemistry, we learned that our simple views of carbon uptake and transport were severely limited, and a new "wave" of ocean science was born. U.S. JGOFS has been supported primarily by the U.S. National Science Foundation in collaboration with the National Oceanic and Atmospheric Administration, the National Aeronautics and Space Administration, the Department of Energy and the Office of Naval Research. U.S. JGOFS, ended in 2005 with the conclusion of the Synthesis and Modeling Project (SMP).
U.S. JGOFS
largerWorkCitation
program
Ocean Time-series Sites
https://osprey.bco-dmo.org/program/562656
Ocean Time-series Sites
Program description text taken from Chapter 1: Introduction from the Global Intercomparability in a Changing Ocean: An International Time-Series Methods Workshop report published following the workshop held November 28-30, 2012 at the Bermuda Institute of Ocean Sciences. The full report is available from the workshop Web site hosted by US OCB: http://www.whoi.edu/website/TS-workshop/home
Decades of research have demonstrated that the ocean varies across a range of time scales, with anthropogenic forcing contributing an added layer of complexity. In a growing effort to distinguish between natural and human-induced earth system variability, sustained ocean time-series measurements have taken on a renewed importance. Shipboard biogeochemical time-series represent one of the most valuable tools scientists have to characterize and quantify ocean carbon fluxes and biogeochemical processes and their links to changing climate (Karl, 2010; Chavez et al., 2011; Church et al., 2013). They provide the oceanographic community with the long, temporally resolved datasets needed to characterize ocean climate, biogeochemistry, and ecosystem change.
The temporal scale of shifts in marine ecosystem variations in response to climate change are on the order of several decades. The long-term, consistent and comprehensive monitoring programs conducted by time-series sites are essential to understand large-scale atmosphere-ocean interactions that occur on interannual to decadal time scales. Ocean time-series represent one of the most valuable tools scientists have to characterize and quantify ocean carbon fluxes and biogeochemical processes and their links to changing climate.
Launched in the late 1980s, the US JGOFS (Joint Global Ocean Flux Study; http://usjgofs.whoi.edu) research program initiated two time-series measurement programs at Hawaii and Bermuda (HOT and BATS, respectively) to measure key oceanographic measurements in oligotrophic waters. Begun in 1995 as part of the US JGOFS Synthesis and Modeling Project, the CARIACO Ocean Time-Series (formerly known as the CArbon Retention In A Colored Ocean) Program has studied the relationship between surface primary production, physical forcing variables like the wind, and the settling flux of particulate carbon in the Cariaco Basin.
The objective of these time-series effort is to provide well-sampled seasonal resolution of biogeochemical variability at a limited number of ocean observatories, provide support and background measurements for process-oriented research, as well as test and validate observations for biogeochemical models. Since their creation, the BATS, CARIACO and HOT time-series site data have been available for use by a large community of researchers.
Data from those three US funded, ship-based, time-series sites can be accessed at each site directly or by selecting the site name from the Projects section below.
Ocean Time-series
largerWorkCitation
program
CARIACO Ocean Time-Series Program
http://www.imars.usf.edu/CAR/index.html
CARIACO Ocean Time-Series Program
<p>Since 1995, the CARIACO Ocean Time-Series (formerly known as the CArbon Retention In A Colored Ocean) Program has studied the relationship between surface primary production, physical forcing variables like the wind, and the settling flux of particulate carbon in the Cariaco Basin. This depression, located on the continental shelf of Venezuela (Map), shows marked seasonal and interannual variation in hydrographic properties and primary production (carbon fixation rates by photosynthesis of planktonic algae).</p>
<p>This peculiar basin is anoxic below ~250 m, due its restricted circulation and high primary production <a href="http://www.imars.usf.edu/CAR/ar.html#Muller-Karger-2001">(Muller-Karger et al., 2001)</a>. CARIACO observations show annual primary production rates exceed 500 gC/m<sup>2</sup>y, of which over 15-20% can be accounted for by events lasting one month or less. Such events are observed in other locations where time series observations are collected, and suggest that prior estimates of regional production based on limited sampling may have been underestimated. The annual primary production rates in the Cariaco Basin are comparable to rates estimated using time series observations for Monterey Bay (460 gC/m<sup>2</sup>y; <a href="http://www.imars.usf.edu/CAR/ar.html#Chavez">Chavez, 1996</a>), and higher than previous rates estimated for Georges Bank, the New York Shelf, and the Oregon Shelf (380, 300, and 190 gC/m<sup>2</sup>y, respectively; <a href="http://www.imars.usf.edu/CAR/ar.html#Walsh-1988">Walsh, 1988</a>).</p>
<p>The Cariaco Basin has long been the center of attention of scientists trying to explain paleoclimate. Due to its high rates of sedimentation (30 to >100 cm/ky; <a href="http://www.imars.usf.edu/CAR/ar.html#Peterson-2000">Peterson et al., 2000</a>) and excellent preservation, the varved sediments of the Cariaco Basin offer the opportunity to study high resolution paleoclimate and better understand the role of the tropics in global climate change ( <a href="http://www.imars.usf.edu/CAR/ar.html#Black">Black et al., 1999;</a> <a href="http://www.imars.usf.edu/CAR/ar.html#Peterson-2000">Peterson et al., 2000;</a> <a href="http://www.imars.usf.edu/CAR/ar.html#Haug-2001">Haug et al., 2001;</a> <a href="http://www.imars.usf.edu/CAR/ar.html#Black-2004">Black et al., 2004;</a> <a href="http://www.imars.usf.edu/CAR/ar.html#Hughen-2004">Hughen et al., 2004</a> ).</p>
<p>Now, the CARIACO program provides a link between the sediment record and processes near the surface of the ocean. Sediment traps maintained by the CARIACO program show that over 5% of autochtonous material reaches 275 m depth, and that nearly 2% reaches 1,400 m. The significance of this flux is that it represents a sink for carbon and that it helps explain the record of ancient climate stored at the bottom of the Cariaco Basin.</p>
<p>Acknowledgements: This work was supported by the National Science Foundation (NSF), the National Aeronautics and Space Administration (NASA), and Venezuela's Fondo Nacional de Ciencia, Tecnología e Innovación (FONACIT). For more information please see this <a href="http://www.imars.usf.edu/CAR/ar.html#Acknowledgments">Acknowledgements</a> link.</p>
CARIACO
largerWorkCitation
project
eng; USA
oceans
CARIACO basin
-64.735
-64.367
10.492
10.683
1995-11-08
2017-01-12
CARIACO basin
0
BCO-DMO catalogue of parameters from Time-series Niskin-bottle sample data from R/V Hermano Gines cruises in the Cariaco Basin from 1995 through 2017 (CARIACO Ocean Time-Series Program)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/769803.rdf
Name: Cruise_number
Units: integer (nnn)
Description: <p>number of cruise</p>
http://lod.bco-dmo.org/id/dataset-parameter/769804.rdf
Name: Cruise_ID_1
Units: alphanumeric
Description: <p>cruise ID for OCB</p>
http://lod.bco-dmo.org/id/dataset-parameter/769805.rdf
Name: Cruise_ID_2
Units: alphanumeric
Description: <p>cruise ID for the CARIACO project</p>
http://lod.bco-dmo.org/id/dataset-parameter/769806.rdf
Name: Leg
Units: integer (n)
Description: <p>number of cruise in the same month</p>
http://lod.bco-dmo.org/id/dataset-parameter/769807.rdf
Name: Day
Units: unitless
Description: <p>day of sampling in dd format</p>
http://lod.bco-dmo.org/id/dataset-parameter/769808.rdf
Name: Month
Units: unitless
Description: <p>month of sampling in mm format</p>
http://lod.bco-dmo.org/id/dataset-parameter/769809.rdf
Name: Year
Units: unitless
Description: <p>year of sampling in yyyy format</p>
http://lod.bco-dmo.org/id/dataset-parameter/769810.rdf
Name: Hydro_cast_no
Units: integer (n)
Description: <p>hydrographic CTD cast number</p>
http://lod.bco-dmo.org/id/dataset-parameter/769811.rdf
Name: ISO_DateTime_start_hc_local
Units: unitless
Description: <p>start date and time of hydrocast in Venezuelan Standard Time (VET) in ISO 8601 format</p>
http://lod.bco-dmo.org/id/dataset-parameter/769812.rdf
Name: ISO_DateTime_end_hc_local
Units: unitless
Description: <p>end date and time of hydrocast in Venezuelan Standard Time (VET) in ISO 8601 format</p>
http://lod.bco-dmo.org/id/dataset-parameter/769813.rdf
Name: Depth_target
Units: meters (m)
Description: <p>depth target (nominal)</p>
http://lod.bco-dmo.org/id/dataset-parameter/769814.rdf
Name: Depth_real
Units: meters (m)
Description: <p>depth of sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/769815.rdf
Name: O2_ml_L
Units: milliliters/liter (ml/L)
Description: <p>dissolved oxygen (average in ml/L)</p>
http://lod.bco-dmo.org/id/dataset-parameter/769816.rdf
Name: q_O2_ml_L
Units: dimensionless
Description: <p>quality flag for O2_(ml/L)</p>
http://lod.bco-dmo.org/id/dataset-parameter/769817.rdf
Name: O2_umol_kg
Units: micromoles/kilogram (μmol/kg)
Description: <p>dissolved oxygen (average in μmol/kg)</p>
http://lod.bco-dmo.org/id/dataset-parameter/769818.rdf
Name: q_O2_umol_kg
Units: dimensionless
Description: <p>quality flag for O2_(μmol/kg)</p>
http://lod.bco-dmo.org/id/dataset-parameter/769819.rdf
Name: NO3_UDO
Units: micromolar (μM)
Description: <p>nitrate UDO (average)</p>
http://lod.bco-dmo.org/id/dataset-parameter/769820.rdf
Name: q_NO3_UDO
Units: dimensionless
Description: <p>quality flag for nitrate UDO</p>
http://lod.bco-dmo.org/id/dataset-parameter/769821.rdf
Name: PO4_UDO
Units: micromolar (μM)
Description: <p>phosphate UDO (average)</p>
http://lod.bco-dmo.org/id/dataset-parameter/769822.rdf
Name: q_PO4_UDO
Units: dimensionless
Description: <p>quality flag for phosphate UDO</p>
http://lod.bco-dmo.org/id/dataset-parameter/769823.rdf
Name: SiO4_UDO
Units: micromolar (μM)
Description: <p>silica UDO</p>
http://lod.bco-dmo.org/id/dataset-parameter/769824.rdf
Name: q_SiO4_UDO
Units: dimensionless
Description: <p>quality flag for silica UDO</p>
http://lod.bco-dmo.org/id/dataset-parameter/769825.rdf
Name: NH4_USF
Units: micromolar (μM)
Description: <p>ammonia USF</p>
http://lod.bco-dmo.org/id/dataset-parameter/769826.rdf
Name: q_NH4_USF
Units: dimensionless
Description: <p>quality flag for ammonia USF</p>
http://lod.bco-dmo.org/id/dataset-parameter/769827.rdf
Name: NO2_USF
Units: micromolar (μM)
Description: <p>nitrite USF</p>
http://lod.bco-dmo.org/id/dataset-parameter/769828.rdf
Name: q_NO2_USF
Units: dimensionless
Description: <p>quality flag for nitrite USF</p>
http://lod.bco-dmo.org/id/dataset-parameter/769829.rdf
Name: NO3_NO2_USF
Units: micromolar (μM)
Description: <p>nitrate plus nitrite USF</p>
http://lod.bco-dmo.org/id/dataset-parameter/769830.rdf
Name: q_NO3_NO3_USF
Units: dimensionless
Description: <p>quality flag for nitrate plus nitrite USF</p>
http://lod.bco-dmo.org/id/dataset-parameter/769831.rdf
Name: PO4_USF
Units: micromolar (μM)
Description: <p>phosphate USF</p>
http://lod.bco-dmo.org/id/dataset-parameter/769832.rdf
Name: q_PO4_USF
Units: dimensionless
Description: <p>quality flag for phosphate USF</p>
http://lod.bco-dmo.org/id/dataset-parameter/769833.rdf
Name: SiO4_USF
Units: micromolar (μM)
Description: <p>silica USF</p>
http://lod.bco-dmo.org/id/dataset-parameter/769834.rdf
Name: q_SiO4_USF
Units: dimensionless
Description: <p>quality flag for silica USF</p>
http://lod.bco-dmo.org/id/dataset-parameter/769835.rdf
Name: pH
Units: ion scale (pH)
Description: <p>pH average total hydrogen (at 25 deg. C) no corrected for dye impurities</p>
http://lod.bco-dmo.org/id/dataset-parameter/769836.rdf
Name: q_pH
Units: dimensionless
Description: <p>quality flag for pH</p>
http://lod.bco-dmo.org/id/dataset-parameter/769837.rdf
Name: Alkalinity_mol_kg
Units: moles/kilogram (mol/kg)
Description: <p>total alkalinity</p>
http://lod.bco-dmo.org/id/dataset-parameter/769838.rdf
Name: q_Alkalinity_mol_kg
Units: dimensionless
Description: <p>quality flag for total alkalinity (mol/kg)</p>
http://lod.bco-dmo.org/id/dataset-parameter/769839.rdf
Name: Alkalinity_umol_kg
Units: micromoles/kilogram (μmol/kg)
Description: <p>total alkalinity</p>
http://lod.bco-dmo.org/id/dataset-parameter/769840.rdf
Name: q_Alkalinity_umol_kg
Units: dimensionless
Description: <p>quality flag for total alkalinity (μmol/kg)</p>
http://lod.bco-dmo.org/id/dataset-parameter/769841.rdf
Name: TCO2
Units: micromoles/kilogram (μmol/kg)
Description: <p>total carbon dioxide in seawater (no corrected)</p>
http://lod.bco-dmo.org/id/dataset-parameter/769842.rdf
Name: q_TCO2
Units: dimensionless
Description: <p>quality flag for total carbon dioxide in seawater (no corrected)</p>
http://lod.bco-dmo.org/id/dataset-parameter/769843.rdf
Name: fCO2
Units: microatmospheres (μatm)
Description: <p>fugacity of CO2 in sea water (no corrected)</p>
http://lod.bco-dmo.org/id/dataset-parameter/769844.rdf
Name: q_fCO2
Units: dimensionless
Description: <p>quality flag for fugacity of CO2 in sea water (no corrected)</p>
http://lod.bco-dmo.org/id/dataset-parameter/769845.rdf
Name: pH_corrected
Units: ion scale (pH)
Description: <p>pH average total hydrogen (at 25 deg. C) corrected for dye impurities</p>
http://lod.bco-dmo.org/id/dataset-parameter/769846.rdf
Name: q_pH_corrected
Units: dimensionless
Description: <p>quality flag for pH_corrected</p>
http://lod.bco-dmo.org/id/dataset-parameter/769847.rdf
Name: TCO2_corrected
Units: micromoles/kilogram (μmol/kg)
Description: <p>total carbon dioxide in seawater (recalculated with corrected pH)</p>
http://lod.bco-dmo.org/id/dataset-parameter/769848.rdf
Name: q_TCO2_corrected
Units: dimensionless
Description: <p>quality flag for total carbon dioxide in seawate recalculated</p>
http://lod.bco-dmo.org/id/dataset-parameter/769849.rdf
Name: fCO2_corrected
Units: microatmospheres (μatm)
Description: <p>fugacity of total carbon dioxide in seawate recalculated</p>
http://lod.bco-dmo.org/id/dataset-parameter/769850.rdf
Name: q_fCO2_corrected
Units: dimensionless
Description: <p>quality flag for ffugacity of total carbon dioxide recalculated</p>
http://lod.bco-dmo.org/id/dataset-parameter/769851.rdf
Name: Salinity_bottles
Units: PSU (PSU)
Description: <p>salinity from salinometer</p>
http://lod.bco-dmo.org/id/dataset-parameter/769852.rdf
Name: q_Salinity_bottles
Units: dimensionless
Description: <p>quality flag for salinity from salinometer</p>
http://lod.bco-dmo.org/id/dataset-parameter/769853.rdf
Name: Salinity_CTD
Units: PSU (PSU)
Description: <p>salinity from CTD</p>
http://lod.bco-dmo.org/id/dataset-parameter/769854.rdf
Name: q_Salinity_CTD
Units: dimensionless
Description: <p>quality flag for salinity from CTD</p>
http://lod.bco-dmo.org/id/dataset-parameter/769855.rdf
Name: Temperature
Units: degrees Celsius (degC (°C))
Description: <p>temperature from CTD ITS-90 scale</p>
http://lod.bco-dmo.org/id/dataset-parameter/769856.rdf
Name: q_Temperature
Units: dimensionless
Description: <p>quality flag for temperature from CTD</p>
http://lod.bco-dmo.org/id/dataset-parameter/769857.rdf
Name: Sigma_t
Units: kilograms/meter^3 (Kg/m^3)
Description: <p>density</p>
http://lod.bco-dmo.org/id/dataset-parameter/769858.rdf
Name: q_Sigma_t
Units: dimensionless
Description: <p>quality flag for density</p>
http://lod.bco-dmo.org/id/dataset-parameter/769859.rdf
Name: TPP
Units: nanomolar (nM)
Description: <p>total particulate phosphorus</p>
http://lod.bco-dmo.org/id/dataset-parameter/769860.rdf
Name: q_TPP
Units: dimensionless
Description: <p>quality flag for total particulate phosphorus</p>
http://lod.bco-dmo.org/id/dataset-parameter/769861.rdf
Name: PIP
Units: nanomolar (nM)
Description: <p>particulate inorganic phosphorus</p>
http://lod.bco-dmo.org/id/dataset-parameter/769862.rdf
Name: q_PIP
Units: dimensionless
Description: <p>quality flag for particulate inorganic phosphorus</p>
http://lod.bco-dmo.org/id/dataset-parameter/769863.rdf
Name: POC_ug_kg
Units: micrograms/liter (μg/kg)
Description: <p>particulate organic carbon</p>
http://lod.bco-dmo.org/id/dataset-parameter/769864.rdf
Name: q_POC_ug_kg
Units: dimensionless
Description: <p>quality flag for particulate organic carbon (μg/kg)</p>
http://lod.bco-dmo.org/id/dataset-parameter/769865.rdf
Name: PON_ug_kg
Units: micrograms/liter (μg/kg)
Description: <p>particulate organic nitrogen</p>
http://lod.bco-dmo.org/id/dataset-parameter/769866.rdf
Name: q_PON_ug_kg
Units: dimensionless
Description: <p>quality flag for particulate organic nitrogen (μg/kg)</p>
http://lod.bco-dmo.org/id/dataset-parameter/769867.rdf
Name: POC_ug_L
Units: micrograms/liter (μg/L)
Description: <p>particulate organic carbon</p>
http://lod.bco-dmo.org/id/dataset-parameter/769868.rdf
Name: q_POC_ug_L
Units: dimensionless
Description: <p>quality flag for particulate organic carbon (μg/L)</p>
http://lod.bco-dmo.org/id/dataset-parameter/769869.rdf
Name: PN_ug_L
Units: micrograms/liter (μg/L)
Description: <p>particulate organic nitrogen</p>
http://lod.bco-dmo.org/id/dataset-parameter/769870.rdf
Name: q_PN_ug_L
Units: dimensionless
Description: <p>quality flag for particulate organic nitrogen (μg/L)</p>
http://lod.bco-dmo.org/id/dataset-parameter/769871.rdf
Name: C_N_particulate
Units: mole/mole (mol/mol)
Description: <p>carbon to nitrogen ratio of particulate C to N</p>
http://lod.bco-dmo.org/id/dataset-parameter/769872.rdf
Name: q_C_N_particulate
Units: dimensionless
Description: <p>quality flag for C to N particulate</p>
http://lod.bco-dmo.org/id/dataset-parameter/769873.rdf
Name: DON
Units: micromolar (μM)
Description: <p>dissolved organic nitrogen USF</p>
http://lod.bco-dmo.org/id/dataset-parameter/769874.rdf
Name: q_DON
Units: dimensionless
Description: <p>quality flag for dissolved organic nitrogen USF</p>
http://lod.bco-dmo.org/id/dataset-parameter/769875.rdf
Name: DOP
Units: micromolar (μM)
Description: <p>dissolved organic phosporus USF</p>
http://lod.bco-dmo.org/id/dataset-parameter/769876.rdf
Name: q_DOP
Units: dimensionless
Description: <p>quality flag for dissolved organic phosporus USF</p>
http://lod.bco-dmo.org/id/dataset-parameter/769877.rdf
Name: DOC
Units: micromolar (μM)
Description: <p>dissolved organic carbon</p>
http://lod.bco-dmo.org/id/dataset-parameter/769878.rdf
Name: q_DOC
Units: dimensionless
Description: <p>quality flag for dissolved organic carbon</p>
http://lod.bco-dmo.org/id/dataset-parameter/769879.rdf
Name: TOC
Units: micromolar (μM)
Description: <p>total organic carbon</p>
http://lod.bco-dmo.org/id/dataset-parameter/769880.rdf
Name: q_TOC
Units: dimensionless
Description: <p>quality flag for total organic carbon</p>
http://lod.bco-dmo.org/id/dataset-parameter/769881.rdf
Name: PrimaryProductivity
Units: milligrams Carbon/meter^3/hour (mgC/m^3/hr)
Description: <p>primary production</p>
http://lod.bco-dmo.org/id/dataset-parameter/769882.rdf
Name: q_PrimaryProductivity
Units: dimensionless
Description: <p>quality flag for primary production</p>
http://lod.bco-dmo.org/id/dataset-parameter/769883.rdf
Name: Chlorophyll
Units: milligrams/meter^3 (mg/m^3)
Description: <p>chlorophyll a</p>
http://lod.bco-dmo.org/id/dataset-parameter/769884.rdf
Name: q_Chlorophyll
Units: dimensionless
Description: <p>quality flag for chlorophyll a</p>
http://lod.bco-dmo.org/id/dataset-parameter/769885.rdf
Name: Phaeopigments
Units: milligrams/meter^3 (mg/m^3)
Description: <p>phaeopigment</p>
http://lod.bco-dmo.org/id/dataset-parameter/769886.rdf
Name: q_Phaeopigments
Units: dimensionless
Description: <p>quality flag for phaeopigment</p>
http://lod.bco-dmo.org/id/dataset-parameter/769887.rdf
Name: Total_Prokaryotes
Units: (cells/L )10^8 ((cells_x_10^8/L))
Description: <p>number of prokaryotes cells</p>
http://lod.bco-dmo.org/id/dataset-parameter/769888.rdf
Name: Bact_Biomass_mgC_m3
Units: milligrams Carbon/meter^3 (mgC/m^3)
Description: <p>bacterial biomass (mgC/m^3)</p>
http://lod.bco-dmo.org/id/dataset-parameter/769889.rdf
Name: Bact_Biomass_uMC
Units: microMolar Carbon (μMC)
Description: <p>bacterial biomass (μMC)</p>
http://lod.bco-dmo.org/id/dataset-parameter/769890.rdf
Name: Bio_cast_no
Units: integer (n)
Description: <p>biological CTD cast number (primary production chlorophyll and phaeopigments)</p>
http://lod.bco-dmo.org/id/dataset-parameter/769891.rdf
Name: ISO_DateTime_start_bc_local
Units: unitless
Description: <p>start date and time of biocast in Venezuelan Standard Time (VET) in ISO 8601 format</p>
http://lod.bco-dmo.org/id/dataset-parameter/769892.rdf
Name: ISO_DateTime_end_bc_local
Units: unitless
Description: <p>end date and time of biocast in Venezuelan Standard Time (VET) in ISO 8601 format</p>
http://lod.bco-dmo.org/id/dataset-parameter/769893.rdf
Name: ISO_DateTime_start_hc_UTC
Units: yyyy-MM-dd'T'HH:mm:ss
Description: <p>start date and time of hydrocast in UTC in ISO 8601 format</p>
http://lod.bco-dmo.org/id/dataset-parameter/769894.rdf
Name: ISO_DateTime_end_hc_UTC
Units: unitless
Description: <p>end date and time of hydrocast in UTC in ISO 8601 format</p>
http://lod.bco-dmo.org/id/dataset-parameter/769895.rdf
Name: ISO_DateTime_start_bc_UTC
Units: unitless
Description: <p>start time of biocast in UTC in ISO 8601 format</p>
http://lod.bco-dmo.org/id/dataset-parameter/769896.rdf
Name: ISO_DateTime_end_bc_UTC
Units: unitless
Description: <p>end time of biocast in UTC in ISO 8601 format</p>
http://lod.bco-dmo.org/id/dataset-parameter/769897.rdf
Name: Latitude
Units: decimal degrees
Description: <p>Latitude of observations with positive values indicating North</p>
http://lod.bco-dmo.org/id/dataset-parameter/769898.rdf
Name: Longitude
Units: decimal dgrees
Description: <p>Longitude of observations with negative values indicating West</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
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https://darchive.mblwhoilibrary.org/bitstream/1912/24228/1/dataset-3093_niskin-bottle-samples__v1.tsv
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https://doi.org/10.1575/1912/bco-dmo.3093.1
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dataset
<div>Hydrocasts: CTD and Rosette Sample</div>
<div>During each cruise, a minimum of four hydrocasts were performed to collect a suite of core monthly observations. Additional hydrocasts were performed for specific process studies. We conducted separate shallow and deep casts to obtain better vertical resolution for chemical observations, and for productivity and pigment observations. Water was collected with a SeaBird rosette equipped with 12 (8 liter) teflon-coated Niskin bottles (bottle springs were also teflon-coated) at 20 depths between the surface and 1310 m. The rosette housed the CTD, which collected continuous profiles of temperature and salinity. The CTD also had a SBE-43 oxygen probe, a Wetlabs ECO fluorometer outfitted for chlorophyll-a estimates, and a C-Star transmissometer (660 nm, Wetlabs). Beam attenuation measurements were added to the time series on its 11th cruise (November 1986) originally using a SeaTech transmissometer. The rosette was controlled with a SeaBird deck unit via conducting cable, but alternatively it had been actuated automatically based on pressure recordings via an Autofire Module (SBE AFM) when breaks in cable conductivity had occurred.</div>
<div><br />
Between November 1995 and September 1996, three separate SBE-19 CTDs were used in repeated casts until a reliable salinity profile was obtained below the oxycline. The SBE-19 model CTDs frequently failed to provide reliable conductivity values below the oxycline in the Cariaco Basin. Starting in September 1996, the SBE-19 CTDs were replaced by SBE-25 CTDs, which provided extremely accurate and reliable data in anoxic waters.<br />
&nbsp;</div>
<div>All CTDs were calibrated at the Sea-Bird factory once per year. The accuracy of the pressure sensor was 3.5 m and had a resolution of 0.7 m. The temperatures accuracy was 0.002°C with a resolution of 0.0003°C. The conductivity accuracy was 0.003 mmho/cm with a resolution of 0.0004 mmho/cm.<br />
&nbsp;</div>
<div>Salinity</div>
<div>Continuous salinity profiles were calculated from the CTD measurements. Discrete salinity samples were analyzed using a Guildline Portasal 8410 salinometer standardized with IAPSO Standard Seawater, with a precision of better than ± 0.003 and a resolution of 0.0003 mS/cm at 15° C and 35 psu, the accuracy was ±0.003 at the same set point temperature as standardization and within -2° and +4°C of ambient. These salinity values were used to check, and when necessary calibrate, the CTD salinity profiles.<br />
&nbsp;</div>
<div>Discrete Oxygen</div>
<div>Continuous dissolved oxygen (O2) profiles were obtained with a SBE-43 Dissolved Oxygen Sensor coupled to the SBE-25 CTD. Discrete oxygen samples were collected in duplicate using glass-stoppered bottles and analyzed by Winkler titration (Strickland and Parsons, 1972, as modified by Aminot, 1983). The analytical precision for discrete oxygen analysis was ±3 mM, based on analysis of duplicate samples, with a detection limit of 5 mM.<br />
&nbsp;</div>
<div>Nutrients</div>
<div>Since CAR-072 (November 2001) all samples had been filtered through a 0.8 µm Nucleopore filter within minutes of collection, as recommended by the JGOFS protocol, and frozen in plastic bottles until analysis at the University of South Florida (USF). Previous to November 2001, nutrients were filtered through a 0.7 µm GF/F filter before freezing. This data was still considered reliable, as tests using glass fiber filters show no significant contamination. The analyses follow the standard techniques described by Strickland and Parsons (1972). USF follows the recommendations of Gordon et al. (1993) for the WOCE WHP project for nutrient analysis.<br />
&nbsp;</div>
<div>Since CAR-069 (August 2001) all silica samples were kept unfrozen; they were refrigerated and kept in the dark. Prior to CAR-069, silicates were frozen and those exhibiting high concentration of silica (&gt; 40µM below 300m in CARIACO) were affected by polymerization. All deep samples that were frozen showed low values due to polymerization loss, except CAR-063 and CAR-068 which showed high values. CAR-069 was analyzed by Yrene Astor at EDIMAR from the separate unfrozen bottlesand at USF from other, frozen, bottles. Unfrozen CAR-069 resulted higher with deep values close to what was expected (e.g. ∼92µM at 1310m).<br />
&nbsp;</div>
<div>Detection limits for CARIACO nutrient analysis</div>
<div>The limits below were determined by calculating the concentrations in triplicate standards, averaging the results within each triplicate group, calculating the standard deviation for each group, averaging the standard deviations, and finally doubling the averages to get the detection limits. These samples were analyzed on an ALPKEM RFA II. Subsequent Cariaco analyses were performed on a Technicon Analyzer II</div>
<div>
<table border="0" cellpadding="0">
<tbody>
<tr>
<td rowspan="2" style="width:165px">
<p><strong>Nutrient Type</strong></p>
</td>
<td style="width:136px">
<p><strong>ALPKEM RFA II</strong></p>
</td>
<td colspan="2" style="width:303px">
<p><strong>Technicon Analyzer II</strong></p>
</td>
</tr>
<tr>
<td style="width:136px">
<p><strong>Detection limits</strong></p>
</td>
<td style="width:142px">
<p><strong>Errors of analysis</strong></p>
</td>
<td style="width:159px">
<p><strong>Detection limits</strong></p>
</td>
</tr>
<tr>
<td style="width:165px">
<p><strong>PO<sub>4 </sub>Phosphate</strong></p>
</td>
<td style="width:136px">
<p>0.03 µmol</p>
</td>
<td style="width:142px">
<p>&lt;0.01 µM</p>
</td>
<td style="width:159px">
<p>0.02 µM</p>
</td>
</tr>
<tr>
<td style="width:165px">
<p><strong>Si(OH)<sub>4</sub> Silica</strong></p>
</td>
<td style="width:136px">
<p>0.14 µmol</p>
</td>
<td style="width:142px">
<p>0.2 µM</p>
</td>
<td style="width:159px">
<p>0.4 µM</p>
</td>
</tr>
<tr>
<td style="width:165px">
<p><strong>NO<sub>3</sub> Nitrate</strong></p>
</td>
<td style="width:136px">
<p>0.06 µmol</p>
</td>
<td style="width:142px">
<p>0.02 µM</p>
</td>
<td style="width:159px">
<p>0.04 µM</p>
</td>
</tr>
<tr>
<td style="width:165px">
<p><strong>NO<sub>2</sub> Nitrite</strong></p>
</td>
<td style="width:136px">
<p>0.02 µmol</p>
</td>
<td style="width:142px">
<p>&lt;0.01 µM</p>
</td>
<td style="width:159px">
<p>0.01 µM</p>
</td>
</tr>
<tr>
<td style="width:165px">
<p><strong>NH<sub>4</sub> Ammonium</strong></p>
</td>
<td style="width:136px">
<p>0.07 µmol</p>
</td>
<td style="width:142px">
<p>0.05 µM</p>
</td>
<td style="width:159px">
<p>0.1 µM</p>
</td>
</tr>
</tbody>
</table>
</div>
<div>&nbsp;</div>
<div>Primary Production</div>
<div>Primary productivity measurements were made using a modified Steeman Nielsen (1952) NaH14CO3 uptake assay. The productivity measurements consisted of in situ incubations of water collected at 8 depths and inoculated with 14C-labeled bicarbonate. One hour before sunrise, a shallow cast was performed to obtain water from 1, 7, 15, 25, 35, 55, 75, and 100 meters. As the productivity cast was taken, a Licor Photosynthetically-Active Radiation (PAR) integrator, placed high above the ship's bridge, was activated. Water was poured directly from the Niskin bottle under low light conditions into 250 ml clear polycarbonate bottles. These bottles had been previously acid-washed, rinsed, and soaked in de-ionized water for over 48 hours. Bottles were rinsed three times before filling, a near total fill (the volume within the bottles was actually 290 ml of sea water). Four clear polycarbonate bottles were filled from each depth. We wrap one inoculated bottle from each depth in aluminum foil to obtain the dark 14-C uptake rates. An extra bottle for 1, 15, 35, and 75 m was filled, but not inoculated, to provide time-zero (t0) filter and seawater blanks. The t0 samples were kept in the dark in the laboratory and were filtered after deploying the floating incubation buoy.<br />
&nbsp;</div>
<div>We inoculated each sample under low light conditions with 1,000 ml (4 mCi) of the 14C sodium bicarbonate working solution. A 200 ml aliquot for counting total added 14C activity was removed from one of the 3 bottles from each depth and placed in a 20 ml glass scintillation vial containing 250 ml ethanolamine. The mixture was held at 5°C until subsequent liquid scintillation analysis on shore. We also placed 50 ml of the 14C working solution in a vial with ethanolamine (250 ml) for reference counting.<br />
&nbsp;</div>
<div>The dark bottle and 3 light bottles were hooked together with a combination of plastic tie wraps and nylon cord, and kept in the dark while preparations were made for deployment of the productivity incubation float. At approximately 07:00 hours, the productivity array was deployed. The entire productivity ensemble was attached to a buoy equipped with a flag and radar reflector.<br />
&nbsp;</div>
<div>Productivity observations were initiated on December 1995. Between December 1995 and November 1996, we incubated samples from 06:00 to 10:00 hours. Starting December 1996, we changed our protocol to incubate between 07:00 and 11:00 hours. This more accurately represents 1/3 of the daily photoperiod and 1/3 of the total energy received in one day on a year-round basis at 10°30'N, as verified with the PAR light sensor.<br />
&nbsp;</div>
<div>Approximately 4 hours after deployment, the productivity array was recovered. We decided to use 4-hour incubation periods due to the potentially high productivity (&gt;1,000 mg/(m²d)) of this continental margin. Sample bottles were detached from the line and placed in labeled, dark plastic bags until filtration. Time and position of recovery were recorded. Maintaining low light conditions, a 50 ml aliquot was withdrawn from each productivity bottle using a 50 ml plastic syringe. This aliquot was filtered onto a 25 mm Whatman GF/F glass fiber filter, maintaining vacuum levels of ∼1/3 atm. The filter was rinsed with 0.25 ml 0.5 N HCl, and placed in a 20 ml glass scintillation vial, covered, and held at 5°C until subsequent processing on shore. At the shore laboratory, immediately upon return and within 15 hours of sample collection, 10 ml of liquid scintillation cocktail were added to the vials with the filters. These vials were refrigerated until they were ready for analysis on a BetaScout (PerkinElmer)scintillation counter.<br />
&nbsp;</div>
<div>Carbon uptake calculations followed the standard formulation outlined in the JGOFS manual (UNESCO, 1994), taking into consideration a (very low) quenching curve. Specifically, we subtracted the blank from all bottles, and then subtracted the dark bottle uptake from the average uptake in the light bottles to correct for non-photoautotrophic carbon fixation or absorption. Dark uptake values had always been very low. A scaling factor (∼3) was applied to convert the hourly production value to a "daily mean hourly average". This factor varies slightly, as it was based on the fraction of the energy received during the incubation period relative to the total energy received in a day. Daily rates were derived by multiplying the hourly rate by 12. Gieskes and Van Bennekom (1973), Peterson (1980), and Carpenter and Lively (1980) review the historical background, problems, and assumptions involved in the application of the radiocarbon technique to aquatic productivity. Muller-Karger (1984) also summarizes the technique and corrections involved.<br />
&nbsp;</div>
<div>pH and Alkalinity</div>
<div>pH samples were collected directly in 10-cm cells and analyzed on board. We measured pH and total Alkalinity estimates using the precise spectrophotometric dye methods developed by Robert-Baldo et al. (1985), Byrne and Breland (1989), and which we modified from Clayton and Byrne (1993) and Breland and Byrne (1993). These methods circumvent the problem that arises when potentiometric electrodes were transferred from dilute buffers to sea water samples due to the sample's high ionic strength. All the pH values were reported in the Master file for CARIACO data at 25 ℃ to avoid the effects of temperature on the solution chemistry. Measurement analytical precision for pHT at 25°C (total_hydrogen_ion_scale) = ±0.003, and for Total_alkalinity (mmol/kg), the precision is = 5 mmol/kg.&nbsp;<br />
&nbsp;</div>
<div>Corrections of pH for dye indicator impurities: The pH method uses the dye meta-cresol purple (mCP) as the pH indicator. The mCP dye used in CARIACO was in its unpurified form. Impurities in the indicator dye may cause uncertainty in measured pH values (Yao et al., 2007). Unpurified forms of the dye absorb significantly at the wavelength of maximum absorption for the acid species, HI- (434 nm) (Liu et al., 2011). The ratio of indicator absorbance at wavelengths 578 (base specie, I2-) and 434 (R = A578/A434) is used to calculate pH. Therefore, the effect of the impurities translates into apparent lower pH calculated values, especially at surface waters where pH &gt; 8.0 (Yao et al., 2007). The effect of the impurities varies from one indicator manufacturer to another, and from different batches of the same manufacturer (Yao et al., 2007). Fortunately, the indicator used for the whole dataset in CARIACO came from the same batch. Hence, a correction for mCP impurities was applied following the method developed by Douglas and Byrne (2017) to each set of data for each cruise. This correction translated to ~ -0.01 units at pH ~ 8.1, decreasing to ~ - 0.008 units at pH ~ 7.6. The corrections were applied to the whole dataset, and values for DIC and fCO2 were recalculated in the Master file. All the pH values were reported in the Master file for CARIACO data at 25 ℃ to avoid the effects of temperature on the solution chemistry.&nbsp;<br />
&nbsp;</div>
<div>Chlorophyll</div>
<div>Chlorophyll sample collection and storage: water samples were collected from Niskin bottles into 1 L dark polyethylene bottles. These samples were immediately filtered through 25 mm Whatman GF/F filters using a vacuum of less than 100 mm Hg. During the upwelling season (approx. January-May) we filtered 250 ml seawater, and during the rest of the year we filtered 500 ml. Three replicates were taken per depth during the upwelling season, but only two were collected when biomass was obviously at its minimum, during the non-upwelling season. Filters were folded in half twice and placed in glass centrifuge tubes, labeled and frozen. Storage time was kept as short as possible (less than a week) before measurement.<br />
&nbsp;</div>
<div>Chlorophyll procedure: after removal from the freezer, the filters were extracted in 10 ml of methanol. The samples were allowed to extract for 24 hours in the refrigerator. Following extraction, samples were centrifuged for 20 minutes to remove debris. The fluorometer (Turner fluorometer model 10-AU-005) was allowed to warm up and stabilize for 30 minutes prior to use. Pure methanol was measured to confirm the zero position. Samples were transferred to 1-cm cells and they were measured directly into the fluorometer (Fo). 100 µl of 0.48N HCl was added to each cell. A second reading was taken from the fluorometer for each cell (Fa). Standardization. The fluorometer was calibrated every year with a commercially available chlorophyll a standard (Σ). The concentration of chlorophyll-an and phaeopigments in the sample were calculated using Yentsh and Menzel (1963) equation, with a specific absorption coefficient of 74.5 (chlorophyll in methanol).<br />
&nbsp;</div>
<div>HPLC</div>
<div>HPLC analysis was restarted in July 2006 (CAR-123). Samples were filtered 47 mm Whatman GF/F filters at 8 depths (1, 7, 15, 25, 35, 55, 75 and 100m). The volume filtered depends on the amount of particles in the water. Replicates were taken at the 1m depth. Filters were stored in aluminum envelopes and stored in the fridge until reaching shore. Once on shore, samples were stored at -40°C until transportation to the US. Horn Point Laboratory (http://www.hpl.umces.edu/) performs the analyses through a collaborative agreement with NASA. The method used was Van Heukelem and Thomas (2001).&nbsp;<br />
&nbsp;</div>
<div>POC and PON</div>
<div>POC and PON sample collection and storage: water samples were collected from Niskin bottles into 2 L dark polyethylene bottles. These samples were immediately filtered through 25 mm Whatman GF/F filters (precombusted for 5 hours at 450°C) using a vacuum of less than 100 mm Hg. Since July 2007 (CAR-135) filters were acidified (10% HCl) after combustion and prior to sample collection. A portion of these filters was used for POP analysis (see below). Filters were placed on expendable tin disks and then into aluminum foil envelops (also precombusted for 5 hours at 450°C) labeled and frozen. In the laboratory, filters were dried at 65°C for 12-15 hours then stored with silica gel.</div>
<div>Measurement: The filters were folded inside a tin disk and analyzed on a Perkin Elmer 2400 Elemental Analyzer. The samples were combusted at 1200-1300°C and then passed through a reduction tube to removes the oxygen added to raise the combustion temperature. Filers were not acid fumed prior to analysis. The C and N were then separated in a chromatographic column and were measured on a Thermal Conductivity Detector. Carbon and nitrogen standards, and blank filters were used to calibrate the data. The accuracy of the instrument was &lt;0.3% and the precision of the instrument was &lt;0.2%. These were published values and we find that we were always within these limits (usually ±0.15% for carbon and ±0.1% for nitrogen). We ran cystine as our standard (29.99% Carbon, 11.66% Nitrogen). The analytical range of the instrument is: Carbon= .001 to 3.6 mg and Nitrogen= 0.001 to 6.0 mg.<br />
&nbsp;</div>
<div>POP</div>
<div>POP was analyzed from the same POC/PON filters. The method used for the SRP analysis was based on Koroleff (1983).<br />
&nbsp;</div>
<div>Dissolved organic Carbon, Nitrogen and Phosphorous (DOC, DON and DOP)</div>
<div>Measurements of DOC were taken since the beginning of the project in November 1995 but suspended in February 2001 (CAR-062) due to irregularity of results. DOC was reinitiated in March 2005 (CAR-110) using a new protocol. DOC samples were collected monthly and analyzed at the Organic Biogeochemistry Lab in the Rosenstiel School of Marine &amp; Atmospheric Science at the University of Miami. Samples were gravity-filtered directly from the Niskin bottles through 45 mm GF/F precombusted filters using acid cleaned polycarbonate in-line filter holder. Immediately after filtration the polyethylene bottles were frozen at -20°C until analysis.<br />
&nbsp;</div>
<div>DON and DOP measurements were added to the regular CARIACO cruises in July 2004 (CAR-101). Samples were filtered through GF/F filters using a specially built vacuum filter rack. The DON method was based on Solorzano and Sharp (1980). This procedure produces a filtered seawater sample for analysis of total dissolved fixed nitrogen (=nitrate + nitrite + ammonium + DON). DON concentration was obtained by difference from nitrate, nitrite, and ammonium measured in the standard nutrient protocol. DOP was analyzed in the same persulfate-oxidized filtrate solution as DON. That solution yields total inorganic phosphate concentration, which was composed of the inorganic phosphate concentration originally in the seawater, plus an additional phosphate concentration due to the conversion of DOP to phosphate. DOP concentration was then obtained by difference from the inorganic phosphate in the unoxidized sample measured through the standard nutrient protocol.</div>
<div>&nbsp;</div>
<div>Optical measurements</div>
<div>In-water measurements: a PRR-600 (Biospherical) was used to retrieve downwelling irradiance and upwelling radiance. From these, PAR, Kd and reflectance can be calculated. Beam attenuation coefficient (Cp) was measured using a C-Star transmissometer (see section Hydrocasts: CTD and Rosette Sample), which provides measurements at 660 nm throughout the entire water column.<br />
&nbsp;</div>
<div>CDOM samples were measured at four depths (1m, 15m, 25m, 50m) by filtration through a 0.2 µm pore size filter and immediately frozen at -20°C. Before being analyzed, they were thawed and re-filtered to eliminate any salt crystals that may had formed. CDOM was measured between 200 and 800 nm, with a 0.3 nm interval, using a dual fiber optic spectrometer (Ocean Optics) equipped with 10-cm quartz cuvettes and distilled water as a blank.<br />
&nbsp;</div>
<div>Above water measurements: a PR-650 (Photoresearch) measures sky radiance (Ls), water leaving radiance (Lw) and total irradiance (Es) at an angle of 30°. From these measurements, remote sensing reflectance (Rrs = Lw/Es) can be calculated and used in satellite sensor (such as MODIS and SeaWiFS) calibration.<br />
&nbsp;</div>
<div>Methods compiled by John Akl, July 2002. Revised November 2005 by Laura Lorenzoni. Revised April 2019 by Digna Rueda-Roa<br />
&nbsp;</div>
<div>The CARIACO Ocean Time-Series Program (November 1995 – January 2017)</div>
<p>For a detailed log for each cruise, please refer to the supplemental document Cruise Data Acquisition Report (https://datadocs.bco-dmo.org/docs/302/CARIACO/data_docs/3092/1/Cruise_data_aquisition_report.xlsx)</p>
Specified by the Principal Investigator(s)
<p>BCO-DMO Processing Notes:<br />
- added conventional header with dataset name, PI name, version date<br />
- modified parameter names to conform with BCO-DMO naming conventions<br />
- added latitude and longitude information from additional LatLon.csv file.<br />
- reformatted the date from yyyymmdd to ISO convention yyyy-mm-dd</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
ALPKEM RFA II
ALPKEM RFA II
PI Supplied Instrument Name: ALPKEM RFA II PI Supplied Instrument Description:These samples were analyzed on an ALPKEM RFA II. Instrument Name: Alpkem RFA300 Instrument Short Name:Alpkem RFA300 Instrument Description: A rapid flow analyser (RFA) that may be used to measure nutrient concentrations in seawater. It is an air-segmented, continuous flow instrument comprising a sampler, a peristaltic pump which simultaneously pumps samples, reagents and air bubbles through the system, analytical cartridge, heating bath, colorimeter, data station, and printer. The RFA-300 was a precursor to the smaller Alpkem RFA/2 (also RFA II or RFA-2). Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0414/
SBE-19
SBE-19
PI Supplied Instrument Name: SBE-19 PI Supplied Instrument Description:Between November 1995 and September 1996, three separate SBE-19 CTDs were used in repeated casts until a reliable salinity profile was obtained below the oxycline. The SBE-19 model CTDs frequently failed to provide reliable conductivity values below the oxycline in the Cariaco Basin. Instrument Name: CTD Sea-Bird SEACAT 19 Instrument Short Name:CTD SBE 19 Instrument Description: The Sea-Bird SBE 19 SEACAT Recorder measures conductivity, temperature, and pressure (depth). The SEACAT is self-powered and self-contained and can be deployed in profiling or moored mode. The SBE 19 SEACAT was replaced in 2001 by the 19plus. more information from Sea-Bird Electronics Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0042/
Perkin Elmer 2400 Elemental Analyzer.
Perkin Elmer 2400 Elemental Analyzer.
PI Supplied Instrument Name: Perkin Elmer 2400 Elemental Analyzer. PI Supplied Instrument Description:Measurement: The filters were folded inside a tin disk and analyzed on a Perkin Elmer 2400 Elemental Analyzer. The samples were combusted at 1200-1300°C and then passed through a reduction tube to removes the oxygen added to raise the combustion temperature. Filers were not acid fumed prior to analysis. The C and N were then separated in a chromatographic column and were measured on a Thermal Conductivity Detector. Carbon and nitrogen standards, and blank filters were used to calibrate the data. The accuracy of the instrument was Instrument Name: Elemental Analyzer Instrument Short Name: Instrument Description: Instruments that quantify carbon, nitrogen and sometimes other elements by combusting the sample at very high temperature and assaying the resulting gaseous oxides. Usually used for samples including organic material. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB01/
HPLC
HPLC
PI Supplied Instrument Name: HPLC PI Supplied Instrument Description:HPLC analysis was restarted in July 2006 (CAR-123). Samples were filtered 47 mm Whatman GF/F filters at 8 depths (1, 7, 15, 25, 35, 55, 75 and 100m). The volume filtered depends on the amount of particles in the water. Replicates were taken at the 1m depth. Filters were stored in aluminum envelopes and stored in the fridge until reaching shore. Once on shore, samples were stored at -40°C until transportation to the US. Horn Point Laboratory (http://www.hpl.umces.edu/) performs the analyses through a collaborative agreement with NASA. The method used was Van Heukelem and Thomas (2001). Instrument Name: High-Performance Liquid Chromatograph Instrument Short Name:HPLC Instrument Description: A High-performance liquid chromatograph (HPLC) is a type of liquid chromatography used to separate compounds that are dissolved in solution. HPLC instruments consist of a reservoir of the mobile phase, a pump, an injector, a separation column, and a detector. Compounds are separated by high pressure pumping of the sample mixture onto a column packed with microspheres coated with the stationary phase. The different components in the mixture pass through the column at different rates due to differences in their partitioning behavior between the mobile liquid phase and the stationary phase. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB11/
Licor Photosynthetically-Active Radiation (PAR) integrator
Licor Photosynthetically-Active Radiation (PAR) integrator
PI Supplied Instrument Name: Licor Photosynthetically-Active Radiation (PAR) integrator PI Supplied Instrument Description:As the productivity cast was taken, a Licor Photosynthetically-Active Radiation (PAR) integrator, placed high above the ship's bridge, was activated. Instrument Name: LI-COR Biospherical PAR Sensor Instrument Short Name:LI-COR Biospherical PAR Instrument Description: The LI-COR Biospherical PAR Sensor is used to measure Photosynthetically Available Radiation (PAR) in the water column. This instrument designation is used when specific make and model are not known. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0074/
BetaScout (PerkinElmer) scintillation counter
BetaScout (PerkinElmer) scintillation counter
PI Supplied Instrument Name: BetaScout (PerkinElmer) scintillation counter PI Supplied Instrument Description:These vials were refrigerated until they were ready for analysis on a BetaScout (PerkinElmer) scintillation counter. Instrument Name: Liquid Scintillation Counter Instrument Short Name:LSC Instrument Description: Liquid scintillation counting is an analytical technique which is defined by the incorporation of the radiolabeled analyte into uniform distribution with a liquid chemical medium capable of converting the kinetic energy of nuclear emissions into light energy. Although the liquid scintillation counter is a sophisticated laboratory counting system used to quantify the activity of particulate emitting (ß and a) radioactive samples, it can also detect the auger electrons emitted from 51Cr and 125I samples.
Liquid scintillation counters are instruments assaying alpha and beta radiation by quantitative detection of visible light produced by the passage of rays or particles through a suitable scintillant incorporated into the sample. Community Standard Description: http://vocab.nerc.ac.uk/collection/L05/current/LAB21/
Niskin Bottle
Niskin Bottle
PI Supplied Instrument Name: Niskin Bottle Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
SeaTech transmissometer
SeaTech transmissometer
PI Supplied Instrument Name: SeaTech transmissometer PI Supplied Instrument Description:Beam attenuation measurements were added to the time series on its 11th cruise (November 1986) originally using a SeaTech transmissometer. Instrument Name: Sea Tech Transmissometer Instrument Short Name:Sea Tech Transmissometer Instrument Description: The Sea Tech Transmissometer can be deployed in either moored or profiling mode to estimate the concentration of suspended or particulate matter in seawater. The transmissometer measures the beam attenuation coefficient in the red spectral band (660 nm) of the laser lightsource over the instrument's path-length (e.g. 20 or 25 cm). This instrument designation is used when specific make and model are not known. The Sea Tech Transmissometer was manufactured by Sea Tech, Inc. (Corvalis, OR, USA). Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0003/
SBE-25
SBE-25
PI Supplied Instrument Name: SBE-25 PI Supplied Instrument Description:Starting in September 1996, the SBE-19 CTDs were replaced by SBE-25 CTDs, which provided extremely accurate and reliable data in anoxic waters. Instrument Name: Sea-Bird SBE 25 Sealogger CTD Instrument Short Name:CTD SBE 25 Instrument Description: The Sea-Bird SBE 25 SEALOGGER CTD is battery powered and is typically used to record data in memory, eliminating the need for a large vessel, electrical sea cable, and on-board computer. All SBE 25s can also operate in real-time, transmitting data via an opto-isolated RS-232 serial port. Temperature and conductivity are measured by the SBE 3F Temperature sensor and SBE 4 Conductivity sensor (same as those used on the premium SBE 9plus CTD). The SBE 25 also includes the SBE 5P (plastic) or 5T (titanium) Submersible Pump and TC Duct. The pump-controlled, TC-ducted flow configuration significantly reduces salinity spiking caused by ship heave, and in calm waters allows slower descent rates for improved resolution of water column features. Pressure is measured by the modular SBE 29 Temperature Compensated Strain-Gauge Pressure sensor (available in eight depth ranges to suit the operating depth requirement). The SBE 25's modular design makes it easy to configure in the field for a wide range of auxiliary sensors, including optional dissolved oxygen (SBE 43), pH (SBE 18 or SBE 27), fluorescence, transmissivity, PAR, and optical backscatter sensors. More information from Sea-Bird Electronics: http:www.seabird.com. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0040/
SBE-43 oxygen probe
SBE-43 oxygen probe
PI Supplied Instrument Name: SBE-43 oxygen probe PI Supplied Instrument Description:The CTD also had a SBE-43 oxygen probe Instrument Name: Sea-Bird SBE 43 Dissolved Oxygen Sensor Instrument Short Name:SBE-43 DO Instrument Description: The Sea-Bird SBE 43 dissolved oxygen sensor is a redesign of the Clark polarographic membrane type of dissolved oxygen sensors. more information from Sea-Bird Electronics Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0036/
Technicon Analyzer II
Technicon Analyzer II
PI Supplied Instrument Name: Technicon Analyzer II PI Supplied Instrument Description:Subsequent Cariaco analyses were performed on a Technicon Analyzer II Instrument Name: Technicon AutoAnalyzer II Instrument Short Name: Instrument Description: A rapid flow analyzer that may be used to measure nutrient concentrations in seawater. It is a continuous segmented flow instrument consisting of a sampler, peristaltic pump, analytical cartridge, heating bath, and colorimeter. See more information about this instrument from the manufacturer. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0291/
Turner fluorometer model 10-AU-005
Turner fluorometer model 10-AU-005
PI Supplied Instrument Name: Turner fluorometer model 10-AU-005 PI Supplied Instrument Description:Chlorophyll procedure: after removal from the freezer, the filters were extracted in 10 ml of methanol. The samples were allowed to extract for 24 hours in the refrigerator. Following extraction, samples were centrifuged for 20 minutes to remove debris. The fluorometer (Turner fluorometer model 10-AU-005) was allowed to warm up and stabilize for 30 minutes prior to use. Pure methanol was measured to confirm the zero position. Samples were transferred to 1-cm cells and they were measured directly into the fluorometer (Fo). 100 µl of 0.48N HCl was added to each cell. A second reading was taken from the fluorometer for each cell (Fa). Standardization. The fluorometer was calibrated every year with a commercially available chlorophyll a standard (Σ). The concentration of chlorophyll-an and phaeopigments in the sample were calculated using Yentsh and Menzel (1963) equation, with a specific absorption coefficient of 74.5 (chlorophyll in methanol). Instrument Name: Turner Designs Fluorometer 10-AU Instrument Short Name:Turner Fluorometer 10-AU Instrument Description: The Turner Designs 10-AU Field Fluorometer is used to measure Chlorophyll fluorescence. The 10AU Fluorometer can be set up for continuous-flow monitoring or discrete sample analyses. A variety of compounds can be measured using application-specific optical filters available from the manufacturer (read more from Turner Designs, turnerdesigns.com, Sunnyvale, CA, USA). Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0393/
C-Star transmissometer
C-Star transmissometer
PI Supplied Instrument Name: C-Star transmissometer PI Supplied Instrument Description:a C-Star transmissometer (660 nm, Wetlabs) Instrument Name: WET Labs {Sea-Bird WETLabs} C-Star transmissometer Instrument Short Name:WL CSTAR Trans Instrument Description: The C-Star transmissometer has a novel monolithic housing with a highly intgrated opto-electronic design to provide a low cost, compact solution for underwater measurements of beam transmittance. The C-Star is capable of free space measurements or flow-through sampling when used with a pump and optical flow tubes. The sensor can be used in profiling, moored, or underway applications. Available with a 6000 m depth rating.
More information on Sea-Bird website: https://www.seabird.com/c-star-transmissometer/product?id=60762467717 Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0160/
Wetlabs ECO fluorometer
Wetlabs ECO fluorometer
PI Supplied Instrument Name: Wetlabs ECO fluorometer PI Supplied Instrument Description:a Wetlabs ECO fluorometer outfitted for chlorophyll-a estimates Instrument Name: WETLabs ECO-FLNTU Instrument Short Name:WETLabs ECO-FLNTU Instrument Description: The ECO FLNTU is a dual-wavelength, single-angle sensor for simultaneously determining both chlorophyll fluorescence and turbidity. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0215/
Cruise: HG93_CARIACO
HG93_CARIACO
B/O Hermano Gines
vessel
B/O Hermano Gines
vessel