http://lod.bco-dmo.org/id/dataset/3229
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2010-08-23
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Biomass of phytoplankton and microzooplankton taxa groups collected from cruises W0306A, W0308C, AT11-17, AT11-30, TUIM14MV, TN200 from the Coastal Waters off Washington State and Vancouver Island; 2003-2006 (ECOHAB-PNW project)
2009-01-30
publication
2009-01-30
revision
BCO-DMO Linked Data URI
2009-01-30
creation
http://lod.bco-dmo.org/id/dataset/3229
Evelyn J. Lessard
University of Washington
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Lessard, E. J. (2009) Biomass of phytoplankton and microzooplankton taxa groups collected from cruises W0306A, W0308C, AT11-17, AT11-30, TUIM14MV, TN200 from the Coastal Waters off Washington State and Vancouver Island; 2003-2006 (ECOHAB-PNW project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 30 January 2009) Version Date 2009-01-30 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/3229 [access date]
Biomass of phytoplankton and microzooplankton taxa groups Dataset Description: ECOHAB/PNW - Biomass of phytoplankton and microzooplankton taxa groups<br><br> Methods and Sampling: <b>Methods for Phototrophic and Heterotrophic Plankton Taxa Sampling, Preservation,<br>
Enumeration and Biomass Estimation</b><br>
(Modified from Frame and Lessard, in press)<br>
<br>
Biomass of phytoplankton and microzooplankton taxa groups was measured in the initial samples<br>
for dilution experiments off the Washington coast taken during six ECOHAB PNW cruises 2003-2006.<br>
<br>
Replicate samples for phytoplankton and microzooplankton identification, enumeration, and sizing<br>
were preserved with either acid Lugol's (5% final conc.) or gluteraldehyde (0.5% final conc.).<br>
Gluteraldehyde-fixed samples were stained with DAPI and proflavin, then filtered onto 0.8&#0181;m and<br>
0.2&#0181;m black polycarbonate membranes (Lessard and Murrell 1996). Slides were stored frozen until<br>
analysis onshore.<br>
<br>
<i>Microscope counts</i><br>
Known volumes of the Lugols-preserved samples were settled in counting chambers for enumeration of<br>
ciliates (including mixotrophs), diatoms and larger dinoflagellates. Counts were performed on a Zeiss<br>
inverted microscope at 250x magnification. Glutarldehyde-preserved samples on 0.8&#0181;m filters were<br>
examined with a Zeiss standard epifluorescence microscope at 400x magnification for enumeration of<br>
microphytoplankton including photosynthetic dinoflagellates (typically <40&#0181;m), cryptophytes, and<br>
an unidentified raphidophyte. Samples on the 0.2&#0181;m filters were examined at 1000x magnification for<br>
cyanobacteria, picoeukaryotes (<2 &#0181;m) and photosynthetic and heterotrophic nanoflagellates (>2-10&#0181;m).<br>
<br>
<i>Biomass estimates</i><br>
Picoplankton (cyanobacteria and picoeukaryotes) were sized using images taken with a QImaging Retiga<br>
EX charged coupled device (CCD) camera and Image Pro Plus software. Dimensions of all other cells<br>
were measured using a computer-aided digitizing system (Roff and Hopcroft 1986). Cell volumes were<br>
calculated using appropriate geometric formulas and carbon was calculated using the carbon to volume<br>
equations of Menden-Deuer and Lessard (2000) for diatoms, nanoplankton and dinoflagellates, and of<br>
Worden et al. (2004) for picoplankton. Ciliate carbon was estimated using the conversion from Putt<br>
and Stoecker (1989).<br>
<br>
<br>
<b>References for further details:</b><br>
Lessard, E.J. and M.C. Murrell (1996), Distribution, abundance and size composition of heterotrophic<br>
dinoflagellates and ciliates in the Sargasso Sea near Bermuda, Deep Sea Research Part I, 43(7), 1045-1065.<br>
<br>
Frame, E.R. Frame and E.J. Lessard. Does the Columbia River Plume influence phytoplankton community<br>
structure along the Washington and Oregon coasts? In press. J. Geophysical Res.<br>
<br>
Menden-Deuer, S., and E. J. Lessard (2000), Carbon to volume relationships for dinoflagellates,<br>
diatoms, and other protist plankton. Limnol. Oceanogr., 45, 569-579.<br>
<br>
Putt, M., and D. K. Stoecker. 1989. An experimentally determined carbon:volume ratio for marine<br>
"oligotrichous" ciliates from estuarine and coastal waters. Limnol. Oceanogr. 34: 1097-1103.<br>
<br>
Roff, J. C. and R. R. Hopcroft (1986), High precision microcomputer based measuring system for<br>
ecological research, Can. J. Fish. Aquat. Sci., 43, 2044-2048.<br>
<br>
Worden, A.Z., J.K. Nolan, and B. Palenik (2004), Assessing the dynamics and ecology of marine picophytoplankton:<br>
the importance of the eukaryotic component, Limnol. Oceanogr., 49(1), 168-179.<br>
<br>
<br>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0234587 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0234587
Funding provided by National Oceanic and Atmospheric Administration (NOAA) Award Number: NA170P2789
completed
Evelyn J. Lessard
University of Washington
206-543-8795
School of Oceanography, University of Washington Box 357940
Seattle
WA
98195
USA
elessard@uw.edu
pointOfContact
asNeeded
Dataset Version: 30 January 2009
Unknown
dilExID
CTD
lat
lon
date
depth
diatom
ciliate
hdino
pdino
crypto
peuk
cyano
pnan
hnan
Cruise
theme
None, User defined
experiment id
station id
latitude
longitude
date
depth
diatom abundance
ciliate Carbon biomass
heterotrophic dinoflagellate Carbon biomass
No BCO-DMO term
Cryptophytes
Cyanobacteria
featureType
BCO-DMO Standard Parameters
W0306A
W0308C
AT11-17
AT11-30
TUIM14MV
TN200
service
Deployment Activity
Pacific North West, Coastal Waters off Washington State and Vancouver Island
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
ECOHAB - Pacific Northwest
https://osprey.bco-dmo.org/project/2094
ECOHAB - Pacific Northwest
<p>ECOHAB-PNW is a 5-year multi-disciplinary project that will study the physiology, toxicology, ecology<br />
and oceanography of toxic Pseudo-nitzschia species off the Pacific Northwest coast.</p>
<p>This program studies the physiology, toxicology, ecology and oceanography of toxic Pseudo-nitzschia<br />
species off the Pacific Northwest coast, a region in which both macro-nutrient supply and current<br />
patterns are primarily controlled by seasonal coastal upwelling processes. Recent studies suggest<br />
that the seasonal Juan de Fuca eddy, a nutrient rich retentive feature off the Washington coast<br />
serves as a "bioreactor" for the growth of phytoplankton, including diatoms of the genus Pseudo-nitzschia.<br />
Existing ship of opportunity data are consistent with the working hypothesis that the seasonal<br />
Juan de Fuca eddy is an initiation site for toxic Pseudo-nitzschia that impact the Washington coast<br />
and that upwelling sites adjacent to the coast are less likely to develop toxicity.</p>
<p>The long-term program goal is to develop a mechanistic basis for forecasting toxic Pseudo-nitzschia<br />
bloom development here and in other similar coastal regions in Eastern Boundary upwelling systems.</p>
<p>Specific study objectives are:<br />
- 1.To determine the physical/biological/chemical factors that make the Juan de Fuca eddy region more<br />
viable for growth and sustenance of toxic Pseudo-nitzschia than the nearshore upwelling zone;<br />
- 2. To determine the combination of environmental factors that regulate the production, accumulation,<br />
and/or release of domoic acid (DA) from Pseudo-nitzschia cells in the field;<br />
- 3. To determine possible transport pathways between DA initiation sites and shellfish beds on the nearby coast.</p>
<p>The scientific operations of this study included obtaining multi-disciplinary data from a large scale grid,<br />
sampling water properties while following a drifter, deployment of surface drifters, satellite imagery,<br />
laboratory studies using water collected at selected sites, and numerical modeling of both the circulation<br />
and chlorophyll concentration. Water samples included macronutrients, iron, particulate and dissolved domoic<br />
acid, Pseudo-nitzschia species and numbers. Experiments were done to estimate growth and grazing rates.<br />
Moored arrays were deployed to provide time series of currents and water properties from May to October,<br />
each year from 2003-2006. Numerical modeling studies on a fine scale grid focused on the seasonal development<br />
of the Juan de Fuca eddy and its change in structure during selected wind conditions. Conditions favorable<br />
to release of phytoplankton from the eddy region were assessed.</p>
<p>After four years of field work the research team is able to describe a possible sequence of events necessary<br />
to ingestion of domoic acid by coastal shellfish:<br />
(1) Plankton must become concentrated in the bloom source region. ECOHAB PNW studies suggest this requires<br />
a period of downwelling-favorable or lightly fluctuating winds.<br />
(2) Next the plankton must undergo stress sufficient to cause an increase in cellular toxin: in the Juan de Fuca<br />
eddy region toxin can be found on any survey of the region in both early and late summer within a 21 day time scale.<br />
(3) Patches of toxic plankton must then escape from the offshore source region. For the Juan de Fuca eddy region<br />
escape is favored during upwelling-favorable wind conditions that allow the geostrophic constraint of the eddy<br />
circulation pattern to be broken.<br />
(4) The patch must move alongshore to sites with shellfish populations, and<br />
(5) must retain its toxicity during the time period of transport. For a toxic source in the Juan de Fuca eddy<br />
this requires southward advection across the shelf, as occurs during periods of upwelling-favorable winds in<br />
summer and early fall. ECOHAB PNW studies show that toxin can be maintained in the 7-14 days required for<br />
transport. For an Oregon source such as Heceta bank to impact the Washington shelf, this requires northward<br />
advection across the shelf, as occurs during periods of downwelling-favorable winds in spring.<br />
(6) Last, the toxic patch must move onshore to coastal beaches and/or estuaries,<br />
(7) where it must remain there for a period sufficient for significant ingestion by shellfish.</p>
<p><strong>Cruises/Platforms:</strong><br />
Cruise = ECOHAB-PNW cruises, numbered sequentially from<br />
Cruise_1 - Cruise_6 as ECOHAB_1 - ECOHAB_6.</p>
<p>Cruise_1=ECOHAB_1, R/V Wecoma, W0306A, June 2-23, 2003 <a href="http://bcodata.whoi.edu/ECOHAB_PNW/ECOHAB_Cruise1_Report.pdf">Cruise Report</a><br />
Cruise_2=ECOHAB_2, R/V Wecoma, W0308C, August 30 - September 19, 2003 <a href="http://bcodata.whoi.edu/ECOHAB_PNW/ECOHAB_Cruise2_Report.pdf">Cruise Report</a><br />
Cruise_3=ECOHAB_3, R/V Atlantis, AT11-17, September 8-28, 2004 <a href="http://bcodata.whoi.edu/ECOHAB_PNW/ECOHAB_Cruise3_Report.pdf">Cruise Report</a><br />
Cruise_4=ECOHAB_4, R/V Atlantis, AT11-30, July 7-27,2005 <a href="http://bcodata.whoi.edu/ECOHAB_PNW/ECOHAB_Cruise4_Report.pdf">Cruise Report</a><br />
Cruise_5=ECOHAB_5, R/V Melville, TUIM14MV, September 2-22, 2005 <a href="http://bcodata.whoi.edu/ECOHAB_PNW/ECOHAB_Cruise5_Report.pdf">Cruise Report</a><br />
Cruise_6=ECOHAB_6, R/V Thomas G. Thompson, TN200, Sept. 11- Oct. 4, 2006 <a href="http://bcodata.whoi.edu/ECOHAB_PNW/ECOHAB_Cruise6_Report.pdf">Cruise Report</a><br />
</p>
ECOHAB-PNW
largerWorkCitation
project
eng; USA
oceans
Pacific North West, Coastal Waters off Washington State and Vancouver Island
2009-01-30
Off the Pacific Northwest coast
0
BCO-DMO catalogue of parameters from Biomass of phytoplankton and microzooplankton taxa groups collected from cruises W0306A, W0308C, AT11-17, AT11-30, TUIM14MV, TN200 from the Coastal Waters off Washington State and Vancouver Island; 2003-2006 (ECOHAB-PNW project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/19623.rdf
Name: dilExID
Units: ?????
Description: <p>Experiment number</p>
http://lod.bco-dmo.org/id/dataset-parameter/19624.rdf
Name: CTD
Units: Text
Description: <p>CTD number</p>
http://lod.bco-dmo.org/id/dataset-parameter/19625.rdf
Name: lat
Units: Decimal degrees North positive
Description: <p>Latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/19626.rdf
Name: lon
Units: Decimal degrees East positive
Description: <p>Longitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/19627.rdf
Name: date
Units: YYYYMMDD
Description: <p>Local date</p>
http://lod.bco-dmo.org/id/dataset-parameter/19628.rdf
Name: depth
Units: meters
Description: <p>Depth of sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/19629.rdf
Name: diatom
Units: microg Carbon l-1
Description: <p>diatom biomass</p>
http://lod.bco-dmo.org/id/dataset-parameter/19630.rdf
Name: ciliate
Units: microg Carbon l-1
Description: <p>heterotrophic and mixotrophic ciliates</p>
http://lod.bco-dmo.org/id/dataset-parameter/19631.rdf
Name: hdino
Units: microg Carbon l-1
Description: <p>heterotrophic dinoflagellates</p>
http://lod.bco-dmo.org/id/dataset-parameter/19632.rdf
Name: pdino
Units: microg Carbon l-1
Description: <p>phototrophic dinoflagellates</p>
http://lod.bco-dmo.org/id/dataset-parameter/19633.rdf
Name: crypto
Units: microg Carbon l-1
Description: <p>cryptophytes</p>
http://lod.bco-dmo.org/id/dataset-parameter/19634.rdf
Name: peuk
Units: microg Carbon l-1
Description: <p>picoeukaryotes <2 µm</p>
http://lod.bco-dmo.org/id/dataset-parameter/19635.rdf
Name: cyano
Units: microg Carbon l-1
Description: <p>cyanobacteria</p>
http://lod.bco-dmo.org/id/dataset-parameter/19636.rdf
Name: pnan
Units: microg Carbon l-1
Description: <p>photosynthetic nanoflagellates </p>
http://lod.bco-dmo.org/id/dataset-parameter/19637.rdf
Name: hnan
Units: microg Carbon l-1
Description: <p>heterotrophic nanoflagellates </p>
http://lod.bco-dmo.org/id/dataset-parameter/23253.rdf
Name: Cruise
Units: unknown
Description: <p> Other name of this deployment</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
8484
https://datadocs.bco-dmo.org/file/g77yq0Wiq9Vp5L/Biomass.csv
Biomass.csv
Primary data file for dataset ID 3229
download
https://osprey.bco-dmo.org/dataset/3229/data/download
download
onLine
dataset
<b>Methods for Phototrophic and Heterotrophic Plankton Taxa Sampling, Preservation,<br>
Enumeration and Biomass Estimation</b><br>
(Modified from Frame and Lessard, in press)<br>
<br>
Biomass of phytoplankton and microzooplankton taxa groups was measured in the initial samples<br>
for dilution experiments off the Washington coast taken during six ECOHAB PNW cruises 2003-2006.<br>
<br>
Replicate samples for phytoplankton and microzooplankton identification, enumeration, and sizing<br>
were preserved with either acid Lugol's (5% final conc.) or gluteraldehyde (0.5% final conc.).<br>
Gluteraldehyde-fixed samples were stained with DAPI and proflavin, then filtered onto 0.8&#0181;m and<br>
0.2&#0181;m black polycarbonate membranes (Lessard and Murrell 1996). Slides were stored frozen until<br>
analysis onshore.<br>
<br>
<i>Microscope counts</i><br>
Known volumes of the Lugols-preserved samples were settled in counting chambers for enumeration of<br>
ciliates (including mixotrophs), diatoms and larger dinoflagellates. Counts were performed on a Zeiss<br>
inverted microscope at 250x magnification. Glutarldehyde-preserved samples on 0.8&#0181;m filters were<br>
examined with a Zeiss standard epifluorescence microscope at 400x magnification for enumeration of<br>
microphytoplankton including photosynthetic dinoflagellates (typically <40&#0181;m), cryptophytes, and<br>
an unidentified raphidophyte. Samples on the 0.2&#0181;m filters were examined at 1000x magnification for<br>
cyanobacteria, picoeukaryotes (<2 &#0181;m) and photosynthetic and heterotrophic nanoflagellates (>2-10&#0181;m).<br>
<br>
<i>Biomass estimates</i><br>
Picoplankton (cyanobacteria and picoeukaryotes) were sized using images taken with a QImaging Retiga<br>
EX charged coupled device (CCD) camera and Image Pro Plus software. Dimensions of all other cells<br>
were measured using a computer-aided digitizing system (Roff and Hopcroft 1986). Cell volumes were<br>
calculated using appropriate geometric formulas and carbon was calculated using the carbon to volume<br>
equations of Menden-Deuer and Lessard (2000) for diatoms, nanoplankton and dinoflagellates, and of<br>
Worden et al. (2004) for picoplankton. Ciliate carbon was estimated using the conversion from Putt<br>
and Stoecker (1989).<br>
<br>
<br>
<b>References for further details:</b><br>
Lessard, E.J. and M.C. Murrell (1996), Distribution, abundance and size composition of heterotrophic<br>
dinoflagellates and ciliates in the Sargasso Sea near Bermuda, Deep Sea Research Part I, 43(7), 1045-1065.<br>
<br>
Frame, E.R. Frame and E.J. Lessard. Does the Columbia River Plume influence phytoplankton community<br>
structure along the Washington and Oregon coasts? In press. J. Geophysical Res.<br>
<br>
Menden-Deuer, S., and E. J. Lessard (2000), Carbon to volume relationships for dinoflagellates,<br>
diatoms, and other protist plankton. Limnol. Oceanogr., 45, 569-579.<br>
<br>
Putt, M., and D. K. Stoecker. 1989. An experimentally determined carbon:volume ratio for marine<br>
"oligotrichous" ciliates from estuarine and coastal waters. Limnol. Oceanogr. 34: 1097-1103.<br>
<br>
Roff, J. C. and R. R. Hopcroft (1986), High precision microcomputer based measuring system for<br>
ecological research, Can. J. Fish. Aquat. Sci., 43, 2044-2048.<br>
<br>
Worden, A.Z., J.K. Nolan, and B. Palenik (2004), Assessing the dynamics and ecology of marine picophytoplankton:<br>
the importance of the eukaryotic component, Limnol. Oceanogr., 49(1), 168-179.<br>
<br>
<br>
Specified by the Principal Investigator(s)
<b>BCO-DMO Processing Notes</b><br>
Generated from original file ECOHAB_PNW_biomass_plankton_taxa_groups.xls<br>contributed to BCO-DMO as a single sheet xls file by Evelyn Lessard
<br><br>
<b>BCO-DMO Edits</b><br>
- Parameter names modified to conform to BCO-DMO convention<br>
- date reformatted to YYYYMMDD<br>
- spaces in Cruise text field converted to "_"<br>
- decimal data values padded to consistent decimal places<br><br>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Cruise: W0306A
W0306A
R/V Wecoma
Community Standard Description
NERC Vocabulary Server
R/V Wecoma
vessel
W0306A
Barbara M. Hickey
University of Washington
http://bcodata.whoi.edu/ECOHAB_PNW/ECOHAB_Cruise1_Report.pdf
Report describing W0306A
Cruise: W0308C
W0308C
R/V Wecoma
Community Standard Description
NERC Vocabulary Server
R/V Wecoma
vessel
W0308C
Barbara M. Hickey
University of Washington
http://bcodata.whoi.edu/ECOHAB_PNW/ECOHAB_Cruise2_Report.pdf
Report describing W0308C
Cruise: AT11-17
AT11-17
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
AT11-17
Barbara M. Hickey
University of Washington
http://bcodata.whoi.edu/ECOHAB_PNW/ECOHAB_Cruise3_Report.pdf
Report describing AT11-17
Cruise: AT11-30
AT11-30
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
AT11-30
Barbara M. Hickey
University of Washington
http://bcodata.whoi.edu/ECOHAB_PNW/ECOHAB_Cruise4_Report.pdf
Report describing AT11-30
Cruise: TUIM14MV
TUIM14MV
R/V Melville
Community Standard Description
International Council for the Exploration of the Sea
R/V Melville
vessel
TUIM14MV
Barbara M. Hickey
University of Washington
http://bcodata.whoi.edu/ECOHAB_PNW/ECOHAB_Cruise5_Report.pdf
Report describing TUIM14MV
Cruise: TN200
TN200
R/V Thomas G. Thompson
Community Standard Description
International Council for the Exploration of the Sea
R/V Thomas G. Thompson
vessel
TN200
Barbara M. Hickey
University of Washington
http://bcodata.whoi.edu/ECOHAB_PNW/ECOHAB_Cruise6_Report.pdf
Report describing TN200
R/V Wecoma
Community Standard Description
NERC Vocabulary Server
R/V Wecoma
vessel
R/V Atlantis
Community Standard Description
International Council for the Exploration of the Sea
R/V Atlantis
vessel
R/V Melville
Community Standard Description
International Council for the Exploration of the Sea
R/V Melville
vessel
R/V Thomas G. Thompson
Community Standard Description
International Council for the Exploration of the Sea
R/V Thomas G. Thompson
vessel