http://lod.bco-dmo.org/id/dataset/3251
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2010-08-23
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
Phytoplankton and microzooplankton taxonomy biomass from R/V Wecoma multiple cruises in the Northeast Pacific coastal waters off states of Washington and Oregon from 2004 to 2006 (RISE project)
2009-03-20
publication
2009-03-20
revision
BCO-DMO Linked Data URI
2009-03-20
creation
http://lod.bco-dmo.org/id/dataset/3251
Evelyn J. Lessard
University of Washington
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: Lessard, E. J. (2009) Phytoplankton and microzooplankton taxonomy biomass from R/V Wecoma multiple cruises in the Northeast Pacific coastal waters off states of Washington and Oregon from 2004 to 2006 (RISE project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 20 March 2009) Version Date 2009-03-20 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/3251 [access date]
Biomass of phytoplankton and microzooplankton taxa groups Dataset Description: RISE - Biomass of phytoplankton and microzooplankton taxa groups<br><br> Methods and Sampling: <b>Methods for Phototrophic and Heterotrophic Plankton Taxa Sampling,<br>
Preservation, Enumeration and Biomass Estimation <br>
(Modified from Frame and Lessard, in press)</b><br>
<br>
Biomass of phytoplankton and microzooplankton taxa groups were measured<br>
in the initial samples for dilution experiments off the Washington and<br>
Oregon coasts taken during four RISE cruises 2004-2006.<br>
<br>
Replicate samples for phytoplankton and microzooplankton identification,<br>
enumeration, and sizing were preserved with either acid Lugol's (5% final<br>
conc.) or gluteraldehyde (0.5% final conc.). Gluteraldehyde-fixed samples<br>
were stained with DAPI and proflavin, then filtered onto 0.8&#0181;m and 0.2&#0181;m<br>
black polycarbonate membranes (Lessard and Murrell 1996). Slides were<br>
stored frozen until analysis onshore.<br>
<br>
<i>Microscope counts</i><br>
Known volumes of the Lugols-preserved samples were settled in counting<br>
chambers for enumeration of ciliates (including mixotrophs), diatoms and<br>
larger dinoflagellates. Counts were performed on a Zeiss inverted microscope<br>
at 250x magnification. Glutarldehyde-preserved samples on 0.8&#0181;m filters<br>
were examined with a Zeiss standard epifluorescence microscope at 400x<br>
magnification for enumeration of microphytoplankton including photosynthetic<br>
dinoflagellates (typically <40&#0181;m), cryptophytes, and an unidentified<br>
raphidophyte. Samples on the 0.2&#0181;m filters were examined at 1000x<br>
magnification for cyanobacteria, picoeukaryotes (<2 &#0181;m) and photosynthetic<br>
and heterotrophic nanoflagellates (>2-10&#0181;m).<br>
<br>
<i>Biomass estimates</i><br>
Picoplankton (cyanobacteria and picoeukaryotes) were sized using images<br>
taken with a QImaging Retiga EX charged coupled device (CCD) camera and<br>
Image Pro Plus software. Dimensions of all other cells were measured<br>
using a computer-aided digitizing system (Roff and Hopcroft 1986).<br>
Cell volumes were calculated using appropriate geometric formulas and<br>
carbon was calculated using the carbon to volume equations of Menden-Deuer<br>
and Lessard (2000) for diatoms, nanoplankton and dinoflagellates, and of<br>
Worden et al. (2004) for picoplankton. Ciliate carbon was estimated using<br>
the conversion from Putt and Stoecker (1989).<br>
<br>
<br>
<b>References for further details:</b><br>
Lessard, E.J. and M.C. Murrell (1996), Distribution, abundance and size composition<br>
of heterotrophic dinoflagellates and ciliates in the Sargasso Sea near Bermuda,<br>
Deep Sea Research Part I, 43(7), 1045-1065.<br>
<br>
Frame, E.R. Frame and E.J. Lessard. Does the Columbia River Plume influence phytoplankton<br>
community structure along the Washington and Oregon coasts? In press. J. Geophysical Res.<br>
<br>
Menden-Deuer, S., and E. J. Lessard (2000), Carbon to volume relationships for<br>
dinoflagellates, diatoms, and other protist plankton. Limnol. Oceanogr., 45, 569-579.<br>
<br>
Putt, M., and D. K. Stoecker. 1989. An experimentally determined carbon:volume ratio<br>
for marine "oligotrichous" ciliates from estuarine and coastal waters.<br>
Limnol. Oceanogr. 34: 1097-1103.<br>
<br>
Roff, J. C. and R. R. Hopcroft (1986), High precision microcomputer based measuring<br>
system for ecological research, Can. J. Fish. Aquat. Sci., 43, 2044-2048.<br>
<br>
Worden, A.Z., J.K. Nolan, and B. Palenik (2004), Assessing the dynamics and ecology<br>
of marine picophytoplankton: the importance of the eukaryotic component,<br>
Limnol. Oceanogr., 49(1), 168-179.<br>
<br>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0239089 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0239089
completed
Evelyn J. Lessard
University of Washington
206-543-8795
School of Oceanography, University of Washington Box 357940
Seattle
WA
98195
USA
elessard@uw.edu
pointOfContact
asNeeded
Dataset Version: 20 March 2009
Unknown
dilExID
Cruise
CTD
Station
lon
lat
date
depth
diatom
ciliate
hdino
pdino
crypto
peuk
cyano
pnan
hnan
theme
None, User defined
experiment id
Cruise identfier
station id
station
longitude
latitude
date
depth
diatom abundance
ciliate Carbon biomass
heterotrophic dinoflagellate Carbon biomass
No BCO-DMO term
Cryptophytes
Cyanobacteria
featureType
BCO-DMO Standard Parameters
W0407A
W0505C
W0508
W0605B
service
Deployment Activity
Northeast Pacific, coastal waters off states of Washington and Oregon
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
River Influences on Shelf Ecosystems
http://www.ocean.washington.edu/rise
River Influences on Shelf Ecosystems
<p><strong>River Influences on Shelf Ecosystems (RISE) - A Study of the Columbia River Plume</strong><br />
<strong>A Multi-Institutional Collaborative Project Sponsored by the National Science Foundation</strong></p>
<p>In 2004 an interdisciplinary study "River Influences on Shelf Ecosystems" (RISE) was initiated to determine the extent to which alongshore gradients in ecosystem productivity might be related to the existence of the massive freshwater plume from the Columbia River. RISE was designed to test three hypotheses: - During upwelling the growth rate of phytoplankton within the Columbia plume exceeds that in nearby areas outside the plume being fueled by the same upwelling nitrate.<br />
- The plume enhances cross-margin transport of plankton and nutrients.<br />
- Plume-specific nutrients (Fe and Si) alter and enhance productivity on adjacent shelves.</p>
<p>Within those constraints, RISE provides the first comprehensive interdisciplinary study of the rates and dynamics governing the mixing of river and coastal waters in an eastern boundary system, as well as the effects of the plume formed by the mixing processes on rates, standing stocks and community structure of plankton in the local ecosystem. The RISE project, includes 4 field and two different numerical model applications. We collected simultaneous measurements of water chemistry, phytoplankton growth and grazing rates, zooplankton populations, water currents, and turbulent mixing. These are being combined with data from satellites, radar, and moorings, as well as detailed numerical simulations, to develop a deeper understanding of this important ecosystem.</p>
<p>The overall RISE sampling strategy was to compare mixing rates, nutrient supply, and phytoplankton production, grazing and community structure within the plume and outside the plume; i.e. on the shelf to the north of the river mouth, presumed more productive, and on the shelf to the south of the river mouth, presumed less productive, as well as in the important "plume lift off" area (the region where the plume loses contact with the bottom) near the river mouth and the plume "near field". The backbone for this project consists of data collected during four cruises that took place in the seasonally high-flow period (May-June) in each of three years (2004-06) and in a low-flow period in the second year (August, 2005). The sampling was spread over three years to attempt to include interannual differences in processes related to wind and river flow variability. The 21-day length of the cruises ensured that a variety of circulation and growth regimes, including upwelling and relaxation/downwelling and neap/spring tides, were observed.</p>
<p>The field studies used two vessels operating simultaneously. The R/V Wecoma obtained primarily biological and chemical rate data: a) at individual stations on cardinal lines north and south of the river mouth (off Grays Harbor, WA and Cape Meares, OR) and near the river mouth; b) at selected process study stations; and c) at fixed stations near the river mouth during strong neap and spring tides (time series). A towed sensor package was used to obtain micronutrient samples near the sea surface on cardinal lines and other selected transects. Underway measurements included macronutrients (N, P, Si), dissolved trace metals (Fe, Mn), supplemented with discrete samples from the underway system (microscopy, FlowCAM and particulate trace metals). At CTD stations vertical profiles (0-200 m where possible; and 500 m at selected stations) of T, S, vertical shear and currents, dissolved O2, in vivo fluorescence, PAR, chlorophyll a, dissolved macronutrients (NO3, NH4, urea, PO4, SiO4), dissolved trace metals, and heterotrophic and autotrophic plankton composition were obtained. Surface drifters were used to follow the mixing of individual plumes and to provide information on surface currents.</p>
<p>On the R/V Pt. Sur, synoptic mesoscale and fine-scale features were sampled with underway measurements of near-surface T, S, velocity, particle size and concentration, PAR, transmissivity and fluorescence and nitrate+nitrite. The Pt. Sur's Triaxus tow fish provided high-resolution sections of T, S, zooplankton (Laser-OPC), PAR and transmissivity, fluorescence, particle size and concentration (LISST-FLOC25X), UV absorption and nitrate (Satlantic ISUS) and radiance/irradiance (7 channels) through the upper water column to 50 m. Rapidly-executed transects of turbulence and fine-structure were also carried out using the Chameleon profiler; these provide full-depth profiles of T, S, optics (880 nm backscatter and fluorescence), turbulence dissipation rates and turbulent fluxes every 1-3 minutes. During selected periods, transects were repeated hourly to capture the high-frequency evolution in the plume's nearfield and river estuary. Acoustics (surface-deployed 1200 kHz ADCP and 120 kHz echosounder) were used to image fine-scale features of the velocity and backscatter fields, resolving fronts, nonlinear internal waves, and turbulent billows.</p>
<p>The temporal context for observed variability was provided by an array of moored sensors deployed in the plume near field as well as on the shelf north and south of the plume (complemented by the pre-existing long-term estuarine and plume stations of the CORIE/SATURN network. To better resolve regional differences, moorings were moved farther north and south to the cardinal sampling lines after the first year of the program. Surface currents were mapped hourly from shore using HF radar with two simultaneously operating arrays, one with a 40 km range and a 2 km range resolution, the other with a 150 km range and a 6 km range resolution. Satellite ocean color, sea surface temperature, turbidity and synthetic aperture radar (SAR) were also obtained when available.</p>
<p>Two modeling systems were developed or enhanced during RISE. The system developed specifically for RISE employed a structured grid model (ROMS) and was used in hindcast mode (MacCready et al., 2008). The CORIE/SATURN modeling system (Baptista, 2006)- based on two unstructured-grid models (SELFE, Zhang and Baptista, 2008; and ELCIRC, Zhang et al., 2004)- was used in both near real-time prognostic mode and multi-year hindcast mode. Both modeling systems incorporated the estuary in the simulation domain (although at different resolutions) and used realistic river, ocean and atmospheric forcing conditions, tidal forcing, and Columbia River estuary forcing. Wind/heat flux model forcing for ROMS was derived from the 4 km MM5 regional wind/heat flux model. SELFE and LCIRC were also forced by MM5. Conditions on open boundaries were provided by ~9 km resolution models from the Navy Research Laboratory (NRL) (NCOM); ROMS used the smaller domain NCOM-CCS NRL model, SELFE and ELCIRC used the larger domain Global-NCOM model. The biological model is a four-box ("NPZD") nitrogen-budget model that tracks nutrients, phytoplankton, zooplankton, and detritus in every cell of the ROMS grid. The rich RISE biological dataset allowed model validation against not just stocks (chlorophyll, microzooplankton, nutrients) but rates (phytoplankton growth and grazing) directly, a level of validation that is seldom possible. These rate observations also allowed the setting of key model parameters (e.g., zooplankton ingestion rate and mortality) empirically (Banas, et al., 2008).</p>
<p><strong>References:</strong><br />
Banas, N. S., P. MacCready, and B. M. Hickey (2008), The Columbia River plume as cross-shelf<br />
exporter and along-coast barrier, doi:10.1016 Cont. Shelf Res., 2008.03.011</p>
<p>Baptista, A. M. (2006), CORIE: the first decade of a coastal-margin collaborative observatory,<br />
Oceans'06, MTS/ IEEE, Boston, MA.</p>
<p>Hickey, B.M., and the RISE PIs. River Influences on Shelf Ecosystems: Introduction to the RISE<br />
Volume, Cont. Shelf Res., in press.</p>
<p>MacCready, P., N. S. Banas, B. H. Hickey, E. P. Dever, and Y. Liu (2008), A model study of<br />
tide- and wind-induced mixing in the Columbia River Estuary and Plume, ,doi:10.1016/j.<br />
Cont. Shelf Res. 2008.03.015.</p>
<p><strong>RISE Cruise Reports and Figures:</strong><br />
<strong>2004 RISE-1</strong><br />
RISE04W1=R/V Wecoma, W0407A, July 8-28, 2004<br />
<a href="http://bcodata.whoi.edu/RISE/CruiseReports/RISE-1_Wecoma_CruiseReport.pdf">Cruise Report</a><br />
<a href="http://bcodata.whoi.edu/RISE/Figures/RISE-1_CruiseTrack.gif" target="_blank">Cruise Track</a><br />
<a href="http://bcodata.whoi.edu/RISE/Figures/RISE-1_Stations_and_Moorings.gif" target="_blank">Stations and Moorings</a><br />
<a href="http://bcodata.whoi.edu/RISE/Figures/RISE-1_WindEvents.gif" target="_blank">Wind Events</a></p>
<p>RISE2004=R/V Point Sur, (tbd), July 8-28, 2004<br />
<a href="http://bcodata.whoi.edu/RISE/CruiseReports/RISE-1_PtSur_CruiseReport.pdf">Cruise Report</a></p>
<p>
<strong>2005 RISE-2</strong><br />
RISE05W2=R/V Wecoma, W0505C, May 29-June 21, 2005<br />
<a href="http://bcodata.whoi.edu/RISE/CruiseReports/RISE-2_Wecoma_CruiseReport.pdf">Cruise Report</a><br />
<a href="http://bcodata.whoi.edu/RISE/Figures/RISE-2_CruiseTrack.gif" target="_blank">Cruise Track</a><br />
<a href="http://bcodata.whoi.edu/RISE/Figures/RISE-2_Stations_and_Moorings.gif" target="_blank">Stations and Moorings</a><br />
<a href="http://bcodata.whoi.edu/RISE/Figures/RISE-2_WindEvents.gif" target="_blank">Wind Events</a></p>
<p>RISE2005a=R/V Point Sur, (tbd), May 29-June 21, 2005<br />
<a href="http://bcodata.whoi.edu/RISE/CruiseReports/RISE-2_PtSur_CruiseReport.pdf">Cruise Report</a></p>
<p>
<strong>2005 RISE-3</strong><br />
RISE05W3=R/V Wecoma, W0508, August 4-August 26, 2005<br />
<a href="http://bcodata.whoi.edu/RISE/CruiseReports/RISE-3_CruiseReport_Daily.pdf">Daily Cruise Report</a><br />
<a href="http://bcodata.whoi.edu/RISE/CruiseReports/RISE-3_CruiseReport_Lessard.pdf">Lessard Cruise Report</a><br />
<a href="http://bcodata.whoi.edu/RISE/CruiseReports/RISE-3_Wecoma_CruiseReport_Peterson.pdf">Peterson/Shaw Zooplankton Report</a><br />
<a href="http://bcodata.whoi.edu/RISE/Figures/RISE-3_CruiseTrack.gif" target="_blank">Cruise Track</a><br />
<a href="http://bcodata.whoi.edu/RISE/Figures/RISE-3_Stations_and_Moorings.gif" target="_blank">Stations and Moorings</a><br />
<a href="http://bcodata.whoi.edu/RISE/Figures/RISE-3_WindEvents.gif" target="_blank">Wind Events</a></p>
<p>RISE2005b=R/V Point Sur, (tbd), August 2-August 27, 2005<br />
<a href="http://bcodata.whoi.edu/RISE/CruiseReports/RISE-3_PtSur_Nash_Cr05_CruiseReport.pdf">Cruise Report</a><br />
<a href="http://bcodata.whoi.edu/RISE/CruiseReports/RISE-3_PtSur_CruiseLog_abbrev.pdf">Cruise Log</a></p>
<p>
<strong>2006 RISE-4</strong><br />
RISE06W4=R/V Wecoma, W0605B, May 21-June 13, 2006<br />
<a href="http://bcodata.whoi.edu/RISE/CruiseReports/RISE-4_Wecoma_CruiseReport_F.pdf">Cruise Report 1</a><br />
<a href="http://bcodata.whoi.edu/RISE/CruiseReports/RISE-4_Wecoma_CruiseReport_F_TDP.pdf">Cruise Report 2</a><br />
<a href="http://bcodata.whoi.edu/RISE/Figures/RISE-4_CruiseTrack.gif" target="_blank">Cruise Track</a><br />
<a href="http://bcodata.whoi.edu/RISE/Figures/RISE-4_Stations_and_Moorings.gif" target="_blank">Stations and Moorings</a><br />
<a href="http://bcodata.whoi.edu/RISE/Figures/RISE-4_WindEvents.gif" target="_blank">Wind Events</a></p>
<p>RISE2006a=Leg 1, R/V Point Sur, (tbd), May 21-May 31, 2006<br />
<a href="http://bcodata.whoi.edu/RISE/CruiseReports/RISE-4_a_PtSur_CruiseReport_Nash.pdf">Cruise Report Leg 1</a><br />
RISE2006b=Leg 2, R/V Point Sur, (tbd), June 2-June 12, 2006<br />
<a href="http://bcodata.whoi.edu/RISE/CruiseReports/RISE-4_b_PtSur_CruiseReport_Jay.pdf">Cruise Report Leg 2</a></p>
RISE
largerWorkCitation
project
eng; USA
oceans
Northeast Pacific, coastal waters off states of Washington and Oregon
2009-03-20
Northeast Pacific, coastal waters off states of Washington and Oregon
0
BCO-DMO catalogue of parameters from Phytoplankton and microzooplankton taxonomy biomass from R/V Wecoma multiple cruises in the Northeast Pacific coastal waters off states of Washington and Oregon from 2004 to 2006 (RISE project)
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/19920.rdf
Name: dilExID
Units: text
Description: <p>Experiment number</p>
http://lod.bco-dmo.org/id/dataset-parameter/19921.rdf
Name: Cruise
Units: text
Description: <p>Cruise name and number</p>
http://lod.bco-dmo.org/id/dataset-parameter/19922.rdf
Name: CTD
Units: Text
Description: <p>CTD number</p>
http://lod.bco-dmo.org/id/dataset-parameter/19923.rdf
Name: Station
Units: Text
Description: <p>Standard station ID</p>
http://lod.bco-dmo.org/id/dataset-parameter/19924.rdf
Name: lon
Units: Decimal degrees
Description: <p>Longitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/19925.rdf
Name: lat
Units: Decimal degrees
Description: <p>Latitude</p>
http://lod.bco-dmo.org/id/dataset-parameter/19926.rdf
Name: date
Units: YYYMMDD
Description: <p>Local date</p>
http://lod.bco-dmo.org/id/dataset-parameter/19927.rdf
Name: depth
Units: meters
Description: <p>Depth of sample</p>
http://lod.bco-dmo.org/id/dataset-parameter/19928.rdf
Name: diatom
Units: microg Carbon l-1
Description: <p>diatom biomass</p>
http://lod.bco-dmo.org/id/dataset-parameter/19929.rdf
Name: ciliate
Units: microg Carbon l-1
Description: <p>heterotrophic and mixotrophic ciliates</p>
http://lod.bco-dmo.org/id/dataset-parameter/19930.rdf
Name: hdino
Units: microg Carbon l-1
Description: <p>heterotrophic dinoflagellates</p>
http://lod.bco-dmo.org/id/dataset-parameter/19931.rdf
Name: pdino
Units: microg Carbon l-1
Description: <p>phototrophic dinoflagellates</p>
http://lod.bco-dmo.org/id/dataset-parameter/19932.rdf
Name: crypto
Units: microg Carbon l-1
Description: <p>cryptophytes</p>
http://lod.bco-dmo.org/id/dataset-parameter/19933.rdf
Name: peuk
Units: microg Carbon l-1
Description: <p>picoeukaryotes <2 µm</p>
http://lod.bco-dmo.org/id/dataset-parameter/19934.rdf
Name: cyano
Units: microg Carbon l-1
Description: <p>cyanobacteria</p>
http://lod.bco-dmo.org/id/dataset-parameter/19935.rdf
Name: pnan
Units: microg Carbon l-1
Description: <p>photosynthetic nanoflagellates </p>
http://lod.bco-dmo.org/id/dataset-parameter/19936.rdf
Name: hnan
Units: microg Carbon l-1
Description: <p>heterotrophic nanoflagellates </p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
7574
https://datadocs.bco-dmo.org/file/P66GwLLU0gjALY/Biomass.csv
Biomass.csv
Primary data file for dataset ID 3251
download
https://osprey.bco-dmo.org/dataset/3251/data/download
download
onLine
dataset
<b>Methods for Phototrophic and Heterotrophic Plankton Taxa Sampling,<br>
Preservation, Enumeration and Biomass Estimation <br>
(Modified from Frame and Lessard, in press)</b><br>
<br>
Biomass of phytoplankton and microzooplankton taxa groups were measured<br>
in the initial samples for dilution experiments off the Washington and<br>
Oregon coasts taken during four RISE cruises 2004-2006.<br>
<br>
Replicate samples for phytoplankton and microzooplankton identification,<br>
enumeration, and sizing were preserved with either acid Lugol's (5% final<br>
conc.) or gluteraldehyde (0.5% final conc.). Gluteraldehyde-fixed samples<br>
were stained with DAPI and proflavin, then filtered onto 0.8&#0181;m and 0.2&#0181;m<br>
black polycarbonate membranes (Lessard and Murrell 1996). Slides were<br>
stored frozen until analysis onshore.<br>
<br>
<i>Microscope counts</i><br>
Known volumes of the Lugols-preserved samples were settled in counting<br>
chambers for enumeration of ciliates (including mixotrophs), diatoms and<br>
larger dinoflagellates. Counts were performed on a Zeiss inverted microscope<br>
at 250x magnification. Glutarldehyde-preserved samples on 0.8&#0181;m filters<br>
were examined with a Zeiss standard epifluorescence microscope at 400x<br>
magnification for enumeration of microphytoplankton including photosynthetic<br>
dinoflagellates (typically <40&#0181;m), cryptophytes, and an unidentified<br>
raphidophyte. Samples on the 0.2&#0181;m filters were examined at 1000x<br>
magnification for cyanobacteria, picoeukaryotes (<2 &#0181;m) and photosynthetic<br>
and heterotrophic nanoflagellates (>2-10&#0181;m).<br>
<br>
<i>Biomass estimates</i><br>
Picoplankton (cyanobacteria and picoeukaryotes) were sized using images<br>
taken with a QImaging Retiga EX charged coupled device (CCD) camera and<br>
Image Pro Plus software. Dimensions of all other cells were measured<br>
using a computer-aided digitizing system (Roff and Hopcroft 1986).<br>
Cell volumes were calculated using appropriate geometric formulas and<br>
carbon was calculated using the carbon to volume equations of Menden-Deuer<br>
and Lessard (2000) for diatoms, nanoplankton and dinoflagellates, and of<br>
Worden et al. (2004) for picoplankton. Ciliate carbon was estimated using<br>
the conversion from Putt and Stoecker (1989).<br>
<br>
<br>
<b>References for further details:</b><br>
Lessard, E.J. and M.C. Murrell (1996), Distribution, abundance and size composition<br>
of heterotrophic dinoflagellates and ciliates in the Sargasso Sea near Bermuda,<br>
Deep Sea Research Part I, 43(7), 1045-1065.<br>
<br>
Frame, E.R. Frame and E.J. Lessard. Does the Columbia River Plume influence phytoplankton<br>
community structure along the Washington and Oregon coasts? In press. J. Geophysical Res.<br>
<br>
Menden-Deuer, S., and E. J. Lessard (2000), Carbon to volume relationships for<br>
dinoflagellates, diatoms, and other protist plankton. Limnol. Oceanogr., 45, 569-579.<br>
<br>
Putt, M., and D. K. Stoecker. 1989. An experimentally determined carbon:volume ratio<br>
for marine "oligotrichous" ciliates from estuarine and coastal waters.<br>
Limnol. Oceanogr. 34: 1097-1103.<br>
<br>
Roff, J. C. and R. R. Hopcroft (1986), High precision microcomputer based measuring<br>
system for ecological research, Can. J. Fish. Aquat. Sci., 43, 2044-2048.<br>
<br>
Worden, A.Z., J.K. Nolan, and B. Palenik (2004), Assessing the dynamics and ecology<br>
of marine picophytoplankton: the importance of the eukaryotic component,<br>
Limnol. Oceanogr., 49(1), 168-179.<br>
<br>
Specified by the Principal Investigator(s)
<b>BCO-DMO Processing Notes</b><br>
Generated from original single sheet xls file contributed to BCO-DMO by Evelyn Lessard<br><br>
<b>BCO-DMO Edits</b><br>
- spaces in Cruise text field converted to "_"<br>
- Date formatted to YYYYMMDD<br>
- decimal data values padded to consistent decimal places<br>
- Cruise changed from "RISE_1,2,3,4" to RISE04W1, etc for consistency with other data sets
<br><br>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
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02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
Cruise: W0407A
W0407A
R/V Wecoma
Community Standard Description
NERC Vocabulary Server
R/V Wecoma
vessel
W0407A
Barbara M. Hickey
University of Washington
http://bcodata.whoi.edu/RISE/CruiseReports/RISE-1_Wecoma_CruiseReport.pdf
Report describing W0407A
Cruise: W0505C
W0505C
R/V Wecoma
Community Standard Description
NERC Vocabulary Server
R/V Wecoma
vessel
W0505C
Barbara M. Hickey
University of Washington
http://bcodata.whoi.edu/RISE/CruiseReports/RISE-2_Wecoma_CruiseReport.pdf
Report describing W0505C
Cruise: W0508
W0508
R/V Wecoma
Community Standard Description
NERC Vocabulary Server
R/V Wecoma
vessel
W0508
Kenneth W. Bruland
University of California-Santa Cruz
http://bcodata.whoi.edu/RISE/CruiseReports/RISE-3_CruiseReport_Daily.pdf
Report describing W0508
Cruise: W0605B
W0605B
R/V Wecoma
Community Standard Description
NERC Vocabulary Server
R/V Wecoma
vessel
W0605B
Barbara M. Hickey
University of Washington
http://bcodata.whoi.edu/RISE/CruiseReports/RISE-4_Wecoma_CruiseReport_F_TDP.pdf
Report describing W0605B
R/V Wecoma
Community Standard Description
NERC Vocabulary Server
R/V Wecoma
vessel