<div><p><strong>Sampling and Analytical Methodology:</strong><br />
Detailed methods for all data collected as part of this study can be found in the publications<br />
arising from this study (references given below). This contains information on analytical<br />
machines and certified standards where applicable.</p>
<p>Sample QA/QC procedures followed those of the Bermuda Atlantic Time-series Study (BATS).<br />
At the point of collection, any leaking niskin bottles were noted on the master cast sheets<br />
and samples were taken from a different niskin fired at the same depth as the leaking bottle.<br />
No data are reported for leaking Niskin bottles. During sample analysis standard curves<br />
and/or certified standards were carefully examined to ensure that they were consistent with<br />
expectations and accurate. If nothing was found, then we examined other data from that<br />
niskin to see if other samples are in question. If no obvious error or problem was found,<br />
the data were considered OK and in the range of environmental data that this study hoped<br />
to observe.</p>
<p><strong>Sample accuracy and precision:</strong><br />
Sample accuracy was assessed by using certified standards, for those measurements where<br />
standards are available (dissolved oxygen, nutrients, salinity). Certified standards were<br />
run with each analytical run and compared to long term control charts for respective analyses.<br />
Samples were not run until certified standards were shown to be accurate for that analytical<br />
run. Sample precision was determined by analyzing replicate samples (not replicate analyses<br />
on the same sample) and therefore is higher than analytical precision due to the inclusion<br />
of sampling error. At the concentrations observed during this study, sample precision was<br />
<5% for stock measurements and <10% for rate measurements. Some analyses, namely dissolved</p>
<!--5--><!--5--><p> oxygen and salinity, were much better and often had a sample precisions <1%. These precision</p>
<!--1--><!--1--><p> estimates are consistent with the long term QA/QC seen with the BATS program.</p>
<p>The provided data files are complete matrices and therefore not every sample (columns) will be<br />
taken from every Niskin fired (rows). Data that were either not collected, or were associated<br />
with leaking Niskins, or were found to be in error for other reasons are denoted by "nd" in the<br />
spreadsheets.</p></div>
Biogeochemistry Data
<div><p>DOP Utilization (ATP3, DOP) Biogeochemistry Data<br />
Biogeochemical data collected on transect cruises studying<br />
Dissolved Organic Phosphorus throughout the western Sargasso Sea.<br />
Data are from several cruises over the span 2006 to 2008.</p>
<p>Note the cruise identifiers for the Atlantic Explorer were originally formatted as XYY## (e.g. X0806 was the 6th cruise in 2008). The data files include cruise IDs of this type. The vessel operator changed the cruise ID syntax several years after the cruise and the official cruise ID syntax was changed to AEYY##. For example, AE0810 should be the same cruise as X0810. One exception for this dataset is that X0804 is cruise ID AE0810.</p>
<p><strong>Related files and references:</strong><br /><em>Detailed information on analyses:</em><br />
Lomas, M.W., Burke, A., Lomas, D.A., Bell, D.W., Shen, C., Ammerman, J.W., Dyhrman, S.T. 2010.<br />
Sargasso Sea phosphorus biogeochemistry: An important role for dissolved organic phosphorus (DOP).<br />
Biogeosciences 7: 695-710.</p>
<p><em>Other published work:</em><br />
Michelou, V.K., Lomas, M.W., Kirchman, D.L. Phosphate and ATP uptake by cyanobacteria and heterotrophic<br />
bacteria in the Sargasso Sea. Limnology and Oceanography, in press.</p>
<p>McLaughlin, K., Sohm, J.A., Cutter, G.A., Lomas, M.W., Paytan, A. 2010. Phosphate cycling in the Sargasso Sea:<br />
Investigation using the oxygen isotopic composition of phosphate, enzyme labeled fluorescence, and turnover times.<br />
Journal of Geophysical Research - Biogeosciences, in press.</p>
<p>Longnecker, K., Lomas, M.W., and Van Mooy, B.A.S. 2010. Characterizing the abundance and diversity of<br />
heterotrophic bacterial cells assimilating phosphate in the sub-tropical North Atlantic Ocean.<br />
Environmental Microbiology, in press.</p>
<p>Orchard, E.D., Ammerman, J.W., Lomas, M.W., Dyhrman, S.T. 2010. Dissolved inorganic and organic phosphorus<br />
uptake in Trichodesmium and the microbial community: The importance of phosphorus ester in the Sargasso Sea.<br />
Limnology and Oceanography, 55:1390-1399.</p>
<p>Casey, J., Lomas, M.W., Michelou, V., Orchard, E.D., Dyhrman, S.T., Ammerman, J.W., and Sylvan, J. 2009.<br />
Phytoplankton taxon-specific orthophosphate (Pi) and ATP uptake in the northwestern Atlantic subtropical gyre.<br />
Aquatic Microbial Ecology, 58:31-44.</p>
<p>Van Mooy, B.A.S., Fredricks, H.F., Pedler, B.F., Dyhrman, S.T., Karl, D.M., Koblizek, M., Lomas, M.W., Moore,<br />
L.R., Moutin, T., Rappé, M.S., and Webb, E.A. 2009. Phytoplankton in the oligotrophic ocean use non-phosphorus lipids in response to phosphorus scarcity. Nature Geosciences, doi:10.1038/nature07659.</p></div>
Biogeochemistry
<div><div><strong>Data Processing:</strong><br />
Most of the data given in this dataset are not derived variables and are calculated using<br />
reasonably standard equations as given in the appropriate reference above. The one exception<br />
is CTD data. Raw CTD data were processed using SBE-Data Processing software using configuration<br />
and calibration files provided by the Shipboard Science technician. Sensors were calibrated<br />
every 6 months. Manual determinations of dissolved oxygen, salinity and HPLC Chlorophyll a,<br />
once passing the above QA/QC steps, were taken as correct. CTD sensor data was regressed against<br />
the appropriate manual variable. In all cases, regression statistics were very strong and linear.<br />
CTD data were corrected to manual measurements using the regression data and it is this corrected<br />
data that is given in the associated data files.
<p>Only nutrient analyses were close to analytical method detection limits (MDL). MDLs were estimated<br />
as 3x the standard deviation of the lowest standard used for the analysis and are ~30nM for nitrate<br />
and phosphate using a standard autoanalyzer. We used the MAGIC co-precipitation method for phosphate<br />
which lowered our MDL to ~1.5nM. Samples below the MDL are reported as calculated for the reason<br />
that they are somewhere between the MDL and a true zero; we consider listing them as either to be<br />
incorrect.</p>
<p>
<strong>BCO-DMO Edits</strong><br />
- Parameter names modified to conform to BCO-DMO convention<br />
- <strong>Note:</strong> Parameter names starting with 33P_xxx changed to P33_xxx for use within system<br />
- date reformatted to YYYYMMDD<br />
- time reformatted to HHMM<br />
- lat/lon padded to 7 decimal places<br />
- added CruiseId column and combined all BGC data into one dataset<br />
- X0606 station 7 year corrected from 2007 to 2006</p></div>
<div>- "-9.99" changed to "nd" (no data) for X0804</div></div>
3354
Biogeochemistry
2010-06-24T12:52:56-04:00
2010-06-24T12:52:56-04:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:3354
Biogeochemistry Data from R/V Atlantic Explorer X0606, X0705, AE0810 in the Western Sargasso Sea roughly 38-20N and 66-43W from 2006-2008 (ATP3 project)
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Lomas, M. W., Ammerman, J., Dyhrman, S. T. (2011) Biogeochemistry Data from R/V Atlantic Explorer X0606, X0705, AE0810 in the Western Sargasso Sea roughly 38-20N and 66-43W from 2006-2008 (ATP3 project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 06 December 2011) Version Date 2011-12-06 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/3354 [access date]
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