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Sample processing and analyses are detailed in the publication listed below. Briefly, 100ml of seawater was collected onto 25mm dia polycarbonate filters (0.22um pore size), rinsed with Tris-buffered saline, flash frozen in liquid N2, and stored at -80C until extraction. DNA was extracted using a combination of 5min of bead beating and 15min heat lysis at 95C. Extracted DNA was used as template of quantitative PCR reactions using primers specifically designed to target different Prochlorococcus ecotypes. Standard curves used for quantitation of field data were derived from DNA extracted from cultured representatives belonging to each ecotype.<\/p>\n
Malmstrom, RR, A Coe, GC Kettler, AC Martiny, J Frias-Lopez, ER Zinser, and SW Chisholm. 2010. Temporal dynamics of Prochlorococcus ecotypes in the Atlantic and Pacific oceans. The ISME Journal. 4(10): 1252-1264<\/p><\/div>","@type":"rdf:HTML"}],"http:\/\/ocean-data.org\/schema\/hasBriefDescription":[{"@value":"Five year time series of Prochlorococcus ecotype abundance at HOT and BATS sites","@language":"en-US"}],"http:\/\/purl.org\/dc\/terms\/description":[{"@value":"
The goal was to collect long-term, high-resolution data on the temporal and spatial variability of Prochlorococcus ecotypes in the Pacific and Atlantic Oceans. The abundance of five Prochlorococcus ecotypes was determined by quantitative PCR at 12 depths every month for 5 years at two locations:<\/p>\n
BATS location (5 nautical mile radius around 31 40'N, 64 10'W)
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Estimated abundances that fell below the lowest value of the standard curve were set to the theoretical detection limit of 0.65 cells\/mL. Samples were excluded if their melt curves contained multiple peaks or peaks different from those in the DNA standards. Missing data points were determined by linear interpolation when abundance estimates were available for the depths immediately above and below the missing value.<\/p>\n
In November 2014, the online data were updated to correct relatively minor errors from HOT cruises 187 and 188. 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