http://lod.bco-dmo.org/id/dataset/3480
eng; USA
utf8
dataset
Highest level of data collection, from a common set of sensors or instrumentation, usually within the same research project
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
2011-05-18
ISO 19115-2 Geographic Information - Metadata - Part 2: Extensions for Imagery and Gridded Data
ISO 19115-2:2009(E)
HPLC analyses of algal pigment concentrations from the CoFeMUG cruise (KN192-05) in the South Atlantic subtropical gyre during 2007
2011-05-18
publication
2011-05-18
revision
BCO-DMO Linked Data URI
2011-05-18
creation
http://lod.bco-dmo.org/id/dataset/3480
Giacomo DiTullio
Grice Marine Laboratory - College of Charleston
principalInvestigator
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
publisher
Cite this dataset as: DiTullio, G. (2011) HPLC analyses of algal pigment concentrations from the CoFeMUG cruise (KN192-05) in the South Atlantic subtropical gyre during 2007. Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 18 May 2011) Version Date 2011-05-18 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/3480 [access date]
HPLC analyses of algal pigment concentrations. Dataset Description: <div>HPLC analyses of algal pigment concentrations from the CoFeMUG cruise.</div>
<div>&nbsp;</div> Methods and Sampling: <p>Algal HPLC pigment samples (2L-4L) were collected from CTD profiles, filtered under low vacuum through GF/F filters, and frozen in LN for onshore analyses. Samples were extracted overnight in cold (-20 degrees C) 100% acetone and analyzed using an Agilent 1100 Model HPLC system equipped with autosampler, photodiode array, and fluorescence detectors. The gradient elution program used was a slight modification of the Zapata et al. gradient elution method (2000). Complete details of the HPLC method are described elsewhere (DiTullio and Geesey 2002). Detection limits for the pigments were on the order of 1 nanogram with a coefficient of variation of &lt;3% for replicate HPLC pigment standard injections. Pigments were isolated from unialgal cultures grown in our lab.</p>
<p>DITULLIO, G. R., AND M. E. GEESEY. 2002. Photosynthetic pigments in marine algae and bacteria, p. 2453 - 2470.<em>In </em> G. Bitton [ed.], The Encyclopedia of Environmental Microbiology. Wiley.</p>
<p>ZAPATA, M., F. RODRIGUEZ, AND J. L. GARRIDO. 2000. Separation of chlorophylls and carotenoids from marine phytoplankton: A new HPLC method using a reversed phase C8 column and pyridine-containing mobile phases. Mar. Ecol. Prog. Ser. 195: 29 - 45.</p>
Funding provided by NSF Division of Ocean Sciences (NSF OCE) Award Number: OCE-0728683 Award URL: https://www.nsf.gov/awardsearch/show-award?AWD_ID=0728683
onGoing
Giacomo DiTullio
Grice Marine Laboratory - College of Charleston
843-953-9196
Hollings Marine Lab 205 Fort Johnson Rd.
Charleston
SC
29412
USA
ditullioj@cofc.edu
pointOfContact
asNeeded
Dataset Version: 18 May 2011
Unknown
station
cast
date
time_start_gmt
time_start_local
lat
lon
depth
Niskin
Amb_Bot
Fluoro_Chl_a_Conc
MgDVP
Chl_c2
Chl_c1
Peridinin
But_19
Fuco
Neo
Prasino
Viola
Hex_19
Diadino
Cis_Fuco
Allo
Diato
Zeaxanthin
Lutein
Crocoxanthin
DV_Chl_a
Chl_a
Chlc_Like
a_Car
b_Car
Chl_c3
Chl_b
Chl_cMVP
Phaeophorobide
Phaeophytin
CTD Sea-Bird SBE 911plus
Niskin bottle
theme
None, User defined
station
cast
date
time_start
time_start_local
latitude
longitude
depth
bottle
No BCO-DMO term
chlorophyll c2
chlorophyll c1
peridinin
lutein
chlorophyll a
chlorophyll c3
chlorophyll b
featureType
BCO-DMO Standard Parameters
CTD Sea-Bird SBE 911plus
Niskin bottle
instrument
BCO-DMO Standard Instruments
KN192-05
service
Deployment Activity
South Atlantic subtropical gyre and Benguela upwelling region
place
Locations
otherRestrictions
otherRestrictions
Access Constraints: none. Use Constraints: Please follow guidelines at: http://www.bco-dmo.org/terms-use Distribution liability: Under no circumstances shall BCO-DMO be liable for any direct, incidental, special, consequential, indirect, or punitive damages that result from the use of, or the inability to use, the materials in this data submission. If you are dissatisfied with any materials in this data submission your sole and exclusive remedy is to discontinue use.
Ocean Carbon and Biogeochemistry
http://us-ocb.org/
Ocean Carbon and Biogeochemistry
The Ocean Carbon and Biogeochemistry (OCB) program focuses on the ocean's role as a component of the global Earth system, bringing together research in geochemistry, ocean physics, and ecology that inform on and advance our understanding of ocean biogeochemistry. The overall program goals are to promote, plan, and coordinate collaborative, multidisciplinary research opportunities within the U.S. research community and with international partners. Important OCB-related activities currently include: the Ocean Carbon and Climate Change (OCCC) and the North American Carbon Program (NACP); U.S. contributions to IMBER, SOLAS, CARBOOCEAN; and numerous U.S. single-investigator and medium-size research projects funded by U.S. federal agencies including NASA, NOAA, and NSF.
The scientific mission of OCB is to study the evolving role of the ocean in the global carbon cycle, in the face of environmental variability and change through studies of marine biogeochemical cycles and associated ecosystems.
The overarching OCB science themes include improved understanding and prediction of: 1) oceanic uptake and release of atmospheric CO2 and other greenhouse gases and 2) environmental sensitivities of biogeochemical cycles, marine ecosystems, and interactions between the two.
The OCB Research Priorities (updated January 2012) include: ocean acidification; terrestrial/coastal carbon fluxes and exchanges; climate sensitivities of and change in ecosystem structure and associated impacts on biogeochemical cycles; mesopelagic ecological and biogeochemical interactions; benthic-pelagic feedbacks on biogeochemical cycles; ocean carbon uptake and storage; and expanding low-oxygen conditions in the coastal and open oceans.
OCB
largerWorkCitation
program
U.S. GEOTRACES
http://www.geotraces.org/
U.S. GEOTRACES
GEOTRACES is a SCOR sponsored program; and funding for program infrastructure development is provided by the U.S. National Science Foundation.
GEOTRACES gained momentum following a special symposium, S02: Biogeochemical cycling of trace elements and isotopes in the ocean and applications to constrain contemporary marine processes (GEOSECS II), at a 2003 Goldschmidt meeting convened in Japan. The GEOSECS II acronym referred to the Geochemical Ocean Section Studies To determine full water column distributions of selected trace elements and isotopes, including their concentration, chemical speciation, and physical form, along a sufficient number of sections in each ocean basin to establish the principal relationships between these distributions and with more traditional hydrographic parameters;
* To evaluate the sources, sinks, and internal cycling of these species and thereby characterize more completely the physical, chemical and biological processes regulating their distributions, and the sensitivity of these processes to global change; and
* To understand the processes that control the concentrations of geochemical species used for proxies of the past environment, both in the water column and in the substrates that reflect the water column.
GEOTRACES will be global in scope, consisting of ocean sections complemented by regional process studies. Sections and process studies will combine fieldwork, laboratory experiments and modelling. Beyond realizing the scientific objectives identified above, a natural outcome of this work will be to build a community of marine scientists who understand the processes regulating trace element cycles sufficiently well to exploit this knowledge reliably in future interdisciplinary studies.
Expand "Projects" below for information about and data resulting from individual US GEOTRACES research projects.
U.S. GEOTRACES
largerWorkCitation
program
Cobalt, Iron and Micro-organisms from the Upwelling zone to the Gyre (GAc01)
https://osprey.bco-dmo.org/project/2067
Cobalt, Iron and Micro-organisms from the Upwelling zone to the Gyre (GAc01)
<p>The geochemistries of dissolved cobalt (Co) and iron (Fe) in the oceanic water column share several characteristics such as extremely low concentrations, redox chemistry, low solubility,and utilization as micronutrients by marine microbes. Iron has been the subject of considerable research focus in recent years due to its role in limiting phytoplankton productivity in oceanic and coastal upwelling environments. Cobalt has been much less studied, but recent data show it may be important in influencing primary productivity or phytoplankton community composition in certain geographical areas.</p>
<p>The CoFeMUG project predated GEOTRACES, so while it is not formally recognized as a GEOTRACES section, it is considered a GEOTRACES-related project and the CoFeMUG data are GEOTRACES compliant.</p>
<p>State-of-the-art geochemical and molecular biological techniques were used to address biogeochemical questions in the South Atlantic, and focus especially on the two trace metals, cobalt and iron. The 27-day cruise in November and December 2007 to the South Atlantic was designed to study cobalt and iron biogeochemistry and focus on four major hypotheses.</p>
<p>(1) Large fluxes of labile cobalt are associated with upwelling systems even in Aeolian dominated environments.<br />
(2) Cobalt and phosphate show correlations in (and only in) surface waters due to micronutrient utilization and rapid remineralization. The slope of the correlation is dependent on the chemical speciation of cobalt.<br />
(3) The absence of Trichodesmium populations in the subtropical and tropical South Atlantic is caused by iron limitation.<br />
(4) Based on work from the California and Peru Upwelling regimes, primary productivity in the Benguela upwelling regime off of South West Africa may be iron limited or iron-cobalt colimited.</p>
<p>A combination of geochemical and biological/molecular analyses were made across an oligotrophic-upwelling transition to examine how changing metal regimes affect the physiology and growth of the important primary producers Trichodesmium and Synechococcus.</p>
<p>CoFeMUG project results are published in:<br />
Noble, Abigail E., Carl H. Lamborg, Dan C. Ohnemus, Phoebe J. Lam, Tyler J. Goepfert, Chris I. Measures, Caitlin H. Frame, Karen L. Casciotti, Giacomo R. DiTullio, Joe Jennings, and Mak A. Saito (2012) Basin-scale inputs of cobalt, iron, and manganese from the Benguela-Angola front to the South Atlantic Ocean. Limnology & Oceanography. Vol. 57(4), July 2012. pgs 989-1010. doi:10.4319/lo.2012.57.4.0989 (<a href="http://www.aslo.org/lo/toc/vol_57/issue_4/0989.pdf">www.aslo.org/lo/toc/vol_57/issue_4/0989.pdf</a>)</p>
CoFeMUG
largerWorkCitation
project
eng; USA
oceans
South Atlantic subtropical gyre and Benguela upwelling region
2011-05-18
South Atlantic subtropical gyre and Benguela upwelling region
0
BCO-DMO catalogue of parameters from HPLC analyses of algal pigment concentrations from the CoFeMUG cruise (KN192-05) in the South Atlantic subtropical gyre during 2007
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
http://lod.bco-dmo.org/id/dataset-parameter/24374.rdf
Name: station
Units: integer
Description: <p>Station number</p>
http://lod.bco-dmo.org/id/dataset-parameter/24375.rdf
Name: cast
Units: integer
Description: <p>Cast number</p>
http://lod.bco-dmo.org/id/dataset-parameter/24376.rdf
Name: date
Units: YYYYMMDD
Description: <p>date (GMT)</p>
http://lod.bco-dmo.org/id/dataset-parameter/24377.rdf
Name: time_start_gmt
Units: HHMM
Description: <p>Start time (GMT)</p>
http://lod.bco-dmo.org/id/dataset-parameter/24378.rdf
Name: time_start_local
Units: HHMM
Description: <p>Start time (Local)</p>
http://lod.bco-dmo.org/id/dataset-parameter/24379.rdf
Name: lat
Units: decimal degrees
Description: <p>Station latitude (South is negative)</p>
http://lod.bco-dmo.org/id/dataset-parameter/24380.rdf
Name: lon
Units: decimal degrees
Description: <p>Station longitude (West is negative)</p>
http://lod.bco-dmo.org/id/dataset-parameter/24381.rdf
Name: depth
Units: meters
Description: <p>Sample depth</p>
http://lod.bco-dmo.org/id/dataset-parameter/24382.rdf
Name: Niskin
Units: integer
Description: <p>Niskin bottle number</p>
http://lod.bco-dmo.org/id/dataset-parameter/24383.rdf
Name: Amb_Bot
Units: integer
Description: <p>Ambient bottle number</p>
http://lod.bco-dmo.org/id/dataset-parameter/24384.rdf
Name: Fluoro_Chl_a_Conc
Units: ug/L
Description: <p>Fluoro_Chl_a_Conc</p>
http://lod.bco-dmo.org/id/dataset-parameter/24385.rdf
Name: MgDVP
Units: ng L-1
Description: <p>MgDVP</p>
http://lod.bco-dmo.org/id/dataset-parameter/24386.rdf
Name: Chl_c2
Units: ng L-1
Description: <p>Chl_c2</p>
http://lod.bco-dmo.org/id/dataset-parameter/24387.rdf
Name: Chl_c1
Units: ng L-1
Description: <p>Chl_c1</p>
http://lod.bco-dmo.org/id/dataset-parameter/24388.rdf
Name: Peridinin
Units: ng L-1
Description: <p>Peridinin</p>
http://lod.bco-dmo.org/id/dataset-parameter/24389.rdf
Name: But_19
Units: ng L-1
Description: <p>19_But</p>
http://lod.bco-dmo.org/id/dataset-parameter/24390.rdf
Name: Fuco
Units: ng L-1
Description: <p>Fuco</p>
http://lod.bco-dmo.org/id/dataset-parameter/24391.rdf
Name: Neo
Units: ng L-1
Description: <p>Neo</p>
http://lod.bco-dmo.org/id/dataset-parameter/24392.rdf
Name: Prasino
Units: ng L-1
Description: <p>Prasino</p>
http://lod.bco-dmo.org/id/dataset-parameter/24393.rdf
Name: Viola
Units: ng L-1
Description: <p>Viola</p>
http://lod.bco-dmo.org/id/dataset-parameter/24394.rdf
Name: Hex_19
Units: ng L-1
Description: <p>19_Hex</p>
http://lod.bco-dmo.org/id/dataset-parameter/24395.rdf
Name: Diadino
Units: ng L-1
Description: <p>Diadino</p>
http://lod.bco-dmo.org/id/dataset-parameter/24396.rdf
Name: Cis_Fuco
Units: ng L-1
Description: <p>Cis_Fuco</p>
http://lod.bco-dmo.org/id/dataset-parameter/24397.rdf
Name: Allo
Units: ng L-1
Description: <p>Allo</p>
http://lod.bco-dmo.org/id/dataset-parameter/24398.rdf
Name: Diato
Units: ng L-1
Description: <p>Diato</p>
http://lod.bco-dmo.org/id/dataset-parameter/24399.rdf
Name: Zeaxanthin
Units: ng L-1
Description: <p>Zeaxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/24400.rdf
Name: Lutein
Units: ng L-1
Description: <p>Lutein</p>
http://lod.bco-dmo.org/id/dataset-parameter/24401.rdf
Name: Crocoxanthin
Units: ng L-1
Description: <p>Crocoxanthin</p>
http://lod.bco-dmo.org/id/dataset-parameter/24402.rdf
Name: DV_Chl_a
Units: ng L-1
Description: <p>DV_Chl_a</p>
http://lod.bco-dmo.org/id/dataset-parameter/24403.rdf
Name: Chl_a
Units: ng L-1
Description: <p>Chl_a</p>
http://lod.bco-dmo.org/id/dataset-parameter/24404.rdf
Name: Chlc_Like
Units: ng L-1
Description: <p>Chlc_Like</p>
http://lod.bco-dmo.org/id/dataset-parameter/24405.rdf
Name: a_Car
Units: ng L-1
Description: <p>a_Car</p>
http://lod.bco-dmo.org/id/dataset-parameter/24406.rdf
Name: b_Car
Units: ng L-1
Description: <p>b_Car</p>
http://lod.bco-dmo.org/id/dataset-parameter/24407.rdf
Name: Chl_c3
Units: ng L-1
Description: <p>Chl_c3</p>
http://lod.bco-dmo.org/id/dataset-parameter/24408.rdf
Name: Chl_b
Units: ng L-1
Description: <p>Chl_b</p>
http://lod.bco-dmo.org/id/dataset-parameter/24409.rdf
Name: Chl_cMVP
Units: ng L-1
Description: <p>Chl_cMVP</p>
http://lod.bco-dmo.org/id/dataset-parameter/24410.rdf
Name: Phaeophorobide
Units: ng L-1
Description: <p>Phaeophorobide</p>
http://lod.bco-dmo.org/id/dataset-parameter/24411.rdf
Name: Phaeophytin
Units: ng L-1
Description: <p>Phaeophytin</p>
GB/NERC/BODC > British Oceanographic Data Centre, Natural Environment Research Council, United Kingdom
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
45407
https://datadocs.bco-dmo.org/file/P66Gwx2co9K4Dy/pigments.csv
pigments.csv
Primary data file for dataset ID 3480
download
https://osprey.bco-dmo.org/dataset/3480/data/download
download
onLine
dataset
<p>Algal HPLC pigment samples (2L-4L) were collected from CTD profiles, filtered under low vacuum through GF/F filters, and frozen in LN for onshore analyses. Samples were extracted overnight in cold (-20 degrees C) 100% acetone and analyzed using an Agilent 1100 Model HPLC system equipped with autosampler, photodiode array, and fluorescence detectors. The gradient elution program used was a slight modification of the Zapata et al. gradient elution method (2000). Complete details of the HPLC method are described elsewhere (DiTullio and Geesey 2002). Detection limits for the pigments were on the order of 1 nanogram with a coefficient of variation of &lt;3% for replicate HPLC pigment standard injections. Pigments were isolated from unialgal cultures grown in our lab.</p>
<p>DITULLIO, G. R., AND M. E. GEESEY. 2002. Photosynthetic pigments in marine algae and bacteria, p. 2453 - 2470.<em>In </em> G. Bitton [ed.], The Encyclopedia of Environmental Microbiology. Wiley.</p>
<p>ZAPATA, M., F. RODRIGUEZ, AND J. L. GARRIDO. 2000. Separation of chlorophylls and carotenoids from marine phytoplankton: A new HPLC method using a reversed phase C8 column and pyridine-containing mobile phases. Mar. Ecol. Prog. Ser. 195: 29 - 45.</p>
Specified by the Principal Investigator(s)
<p>Standard curves relating pigment concentration to peak area were run for each pigment.</p>
<p>Related files and references:<br />
DITULLIO, G. R., AND M. E. GEESEY. 2002. Photosynthetic pigments in marine algae and bacteria, p. 2453 - 2470.<em>In </em>G. Bitton [ed.], The Encyclopedia of Environmental Microbiology. Wiley.</p>
<p>ZAPATA, M., F. RODRIGUEZ, AND J. L. GARRIDO. 2000. Separation of chlorophylls and carotenoids from marine phytoplankton: A new HPLC method using a reversed phase C8 column and pyridine-containing mobile phases. Mar. Ecol. Prog. Ser. 195: 29 - 45.</p>
<p><strong>BCO-DMO Processing Notes</strong><br />
Generated from original .xls file 'CoFeMug Station Pigs.xls' contributed by Tyler Cyronak<br />
<br />
<strong>BCO-DMO Edits</strong><br />
- Parameter names modified to conform to BCO-DMO convention<br />
- Date reformatted to YYYYMMDD<br />
- Time (GMT and Local) reformatted to HHMM<br />
- Blank lines removed<br />
- lat deg/min and lon deg/min columns removed (lat/lon in decimal degrees preserved)<br />
- 'nd' (no data) value inserted in blank cells<br />
- '-999' bad data flag replaced with 'nd'</p>
Specified by the Principal Investigator(s)
asNeeded
7.x-1.1
Biological and Chemical Oceanography Data Management Office (BCO-DMO)
Unavailable
508-289-2009
WHOI MS#36
Woods Hole
MA
02543
USA
info@bco-dmo.org
http://www.bco-dmo.org
Monday - Friday 8:00am - 5:00pm
For questions regarding this resource, please contact BCO-DMO via the email address provided.
pointOfContact
CTD Sea-Bird SBE 911plus
CTD Sea-Bird SBE 911plus
PI Supplied Instrument Name: CTD Sea-Bird SBE 911plus Instrument Name: CTD Sea-Bird SBE 911plus Instrument Short Name:CTD SBE 911plus Instrument Description: The Sea-Bird SBE 911 plus is a type of CTD instrument package for continuous measurement of conductivity, temperature and pressure. The SBE 911 plus includes the SBE 9plus Underwater Unit and the SBE 11plus Deck Unit (for real-time readout using conductive wire) for deployment from a vessel. The combination of the SBE 9 plus and SBE 11 plus is called a SBE 911 plus. The SBE 9 plus uses Sea-Bird's standard modular temperature and conductivity sensors (SBE 3 plus and SBE 4). The SBE 9 plus CTD can be configured with up to eight auxiliary sensors to measure other parameters including dissolved oxygen, pH, turbidity, fluorescence, light (PAR), light transmission, etc.). more information from Sea-Bird Electronics Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0058/
Niskin bottle
Niskin bottle
PI Supplied Instrument Name: Niskin bottle Instrument Name: Niskin bottle Instrument Short Name:Niskin bottle Instrument Description: A Niskin bottle (a next generation water sampler based on the Nansen bottle) is a cylindrical, non-metallic water collection device with stoppers at both ends. The bottles can be attached individually on a hydrowire or deployed in 12, 24, or 36 bottle Rosette systems mounted on a frame and combined with a CTD. Niskin bottles are used to collect discrete water samples for a range of measurements including pigments, nutrients, plankton, etc. Community Standard Description: http://vocab.nerc.ac.uk/collection/L22/current/TOOL0412/
Cruise: KN192-05
KN192-05
R/V Knorr
Community Standard Description
International Council for the Exploration of the Sea
R/V Knorr
vessel
KN192-05
Mak A. Saito
Woods Hole Oceanographic Institution
http://bcodata.whoi.edu/CoFeMUG/CruiseReport_KN192-5.pdf
Report describing KN192-05
R/V Knorr
Community Standard Description
International Council for the Exploration of the Sea
R/V Knorr
vessel