<div><p>Samples were collected in triplicate with a surface water pump (unless depth was specified, then it was collected by CTD), filtered with 0.2um sterivex filters (Millipore Cat #: SVGP 010 50) and fixed with 0.5% final concentration gluteraldehyde. Samples were frozen in liquid nitrogen, and stored at -80.</p>
<p>After thawed, SYBR Gold and stock beads were added to the sample as done in <em>Brussaard 2004</em>. Samples were diluted to get ~200-1000 events per second on a BD FACSCaliber Flow Cytometer looking at fluorescence and side scatter. <strong>Contact for Brian Zielinski for journal-quality methods if needed (<a href="mailto:bzielins@mail.usf.edu">bzielins@mail.usf.edu</a>).</strong></p>
<p>All the stations represent 2-3 true replicates, each replicate consisted of three separate readings. The reason not all of the stations had 3 replicates was because one day the machine malfunctioned and all the samples to be run that day needed to be discarded. The counts usually are very accurate and precise; however it seems that with these samples the precision was not as good as I would hope to see. This can be caused by two things. 1) small organics/cell debris from the plume water could have been included in the viral counts, or 2) I have only briefly used these methods previously and these counts were taken along my learning curve.</p>
<p><strong>Related files and references:</strong><br />
1. Brussaard CPD (2004) Optimization of procedures for counting viruses by flow cytometry. Applied and Environmental Microbiology 70: 1506.</p></div>
Viral Counts
<div><p>Viral counts determined by flow-cytometry</p></div>
Viral Counts - AN10
<div><p><img alt="" src="https://datadocs.bco-dmo.org/d2/ANACONDAS/VirusEquation.gif" /></p>
<p><strong>Related files and references:</strong><br />
1. Brussaard CPD (2004) Optimization of procedures for counting viruses by flow cytometry. Applied and Environmental Microbiology 70: 1506.</p></div>
3495
Viral Counts - AN10
2011-06-22T12:02:17-04:00
2011-06-22T12:02:17-04:00
2023-07-07T16:10:26-04:00
urn:bcodmo:dataset:3495
Viral Counts from R/V Knorr KN197-08 in the Amazon River plume, NE coast of South America from May to June 2010 (ANACONDAS project)
false
Paul, J. (2011) Viral Counts from R/V Knorr KN197-08 in the Amazon River plume, NE coast of South America from May to June 2010 (ANACONDAS project). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 22 June 2011) Version Date 2011-06-22 [if applicable, indicate subset used]. http://lod.bco-dmo.org/id/dataset/3495 [access date]
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22 June 2011
false
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