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&lt;p&gt;This project aims to apply these two methodological advances in order to obtain the next generation of N uptake measurements. Phytoplankton and bacteria specific uptake rates will be measured via the FCM technique, and the individual groups or species of phytoplankton or bacteria will be interrogated for N uptake via DNA SIP. These tools will be applied across the well-characterized nutrient gradient found in Chesapeake Bay during one summer cruise and one winter cruise. Phytoplankton, bacterial, and archaeal populations will be characterized along the sampling transect via multiplexed pyrosequencing technology. N uptake will be measured for inorganic (NH4+, NO3-, and NO2-) and organic N sources (15N and 14C urea dual-labeled and amino acids) as substrates. The investigators hypothesize that phytoplankton will derive a larger percentage of their N nutrition from organic forms along the transect (i.e. North to South), as competition with bacteria for ammonium increases. DNA SIP will be applied to specific dominant phytoplankton and bacterial populations in order to investigate their N nutrition. By applying this unique combination of methodologies, the project will provide unprecedented community, group and species level resolution of N uptake in Chesapeake Bay and will furnish us with an improved understanding of N cycling in the Bay and marine systems as a whole.&lt;/p&gt;
&lt;p&gt;Related Publication: Wawrik, B;  Callaghan, AV;  Bronk, DA. &quot;Use of Inorganic and Organic  Nitrogen by Synechococcus spp. and Diatoms on the West Florida Shelf as  Measured Using Stable Isotope Probing,&quot; &lt;i&gt;APPLIED AND ENVIRONMENTAL MICROBIOLOGY&lt;/i&gt;, v.75, 2009, p. 6662-6670. &lt;a href=&quot;http://gateway.isiknowledge.com/gateway/Gateway.cgi?GWVersion=2&amp;amp;SrcApp=NSF_IR&amp;amp;SrcAuth=NSF&amp;amp;DestLinkType=FullRecord&amp;amp;KeyUT=000271161700005&amp;amp;DestApp=WOS_CPL&quot;&gt;View record at Web of Science&lt;/a&gt;&lt;/p&gt;
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