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&lt;p&gt;Cell membranes make up 10-25% of the carbon biomass in the upper ocean. These important structural components of planktonic cells are dominated by intact polar lipids (IPLs), and a significant fraction of the organic carbon that is exported from the upper ocean is derived from IPLs. The primary tool for analyzing IPLs has been the gas chromatographic (GC) analysis of their constituent fatty acids. This approach has provided many valuable fatty acid biomarkers for specific groups of planktonic organisms. Yet GC is insensitive to an immense degree of structural diversity associated with the larger IPL molecules since fatty acids must be cleaved from polar &quot;headgroups&quot; prior to GC analysis.&lt;/p&gt;
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&lt;p&gt;The research team will apply new high performance liquid chromatography/mass spectrometry (HPLC/MS) methods that allow IPLs to be identified and quantified while still in intact form. This approach has revealed a broad and unrecognized diversity of IPL molecules in the upper ocean. The source organisms of these IPLs are largely unknown, representing a significant gap in our understanding of the upper ocean carbon cycle. Furthermore, IPLs have an immense potential as new biogeochemical tracers for specific groups of microbial plankton.&lt;/p&gt;
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