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            <gmx:Anchor xlink:href="http://lod.bco-dmo.org/id/dataset/3670.rdf" xlink:actuate="onRequest">Algae, bacteria, DOC, inorganic and organic nutrients collected from the Richard B Gump Research Station at Moorea LTER from 2010-2011 (MCR LTER and Coral DOM projects)</gmx:Anchor>
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            <gco:CharacterString>Cite this dataset as: Carlson, C. (2013) Algae, bacteria, DOC, inorganic and organic nutrients collected from the Richard B Gump Research Station at Moorea LTER from 2010-2011 (MCR LTER and Coral DOM projects). Biological and Chemical Oceanography Data Management Office (BCO-DMO). (Version 2) Version Date 2013-12-21 [if applicable, indicate subset used]. doi:10.1575/1912/bco-dmo.3670.2 [access date]</gco:CharacterString>
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        <gco:CharacterString>Moorea Coral Reef algae, bacteria, DOC, inorganic and organic nutrients Dataset Description: &amp;lt;p&amp;gt;This dataset contains experimental and survey biogeochemical and microbial data from samples collected at the Moorea Coral Reef LTER site (French Polynesia). Goals of the study were to investigate how benthic primary producers directly affect seawater chemistry (DO and DOC) and to examine impacts of released DOC on reef bacterioplankton growth and respiration. Rates of photosynthesis, respiration, and DOC relsease were assessed for several benthic reef organisms (Haas et al. 2011).&amp;lt;/p&amp;gt; Methods and Sampling: &amp;lt;p&amp;gt;&amp;lt;strong&amp;gt;2010 and 2011 Data Acquisition:&amp;lt;/strong&amp;gt;&amp;lt;br /&amp;gt;
Sampling from small boats occurred along transects at the Moorea Coral Reef LTER sites during September 2010 and September 2011. Benthic producers were collected from water depths of 1.0 to 1.5 meters at back and fringe reef locations. Bacterioplankton abundance was determined using flow cytometry (Nelson et al. 2011). DOC was measured by a high-temperature TOC analyzer according to methods of Carlson et al. (2010). To assess DOC release by primary producers, each benthic species was incubated using the method of Herndl and Velimirov (Haas et al. 2011). To determine if DOC released by primary producers affects mirobial populations, ~48 hour dark dilution culture incubations were performed to measure changes in concentration of DO, DOC, and bacterioplankton over time (Haas et el. 2011).&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;For more details on methodology, quality assurance and control procedures, and precision and accuracy of methods used, see the following references (full citations are below):&amp;lt;br /&amp;gt;
Bacterioplankton flow cytometry methods: Nelson et al. 2011.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Experimental designs, dissolved oxygen, pH, temperature, and specimen surface area methods: Haas et al. 2011,&amp;amp;nbsp;Herndl &amp;amp;amp; Velimirov B. 1986.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Dissolved organic carbon method: Carlson et al. 2010.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Chlorophyll method: Smith et al. 1981.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Dissolved inorganic nutrients, particulate organic nutrients, and isotope ratios methodological references, calibrations, precision, and accuracy are detailed at the &amp;lt;a href=&amp;quot;http://www.msi.ucsb.edu/services/analytical-lab&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;UCSB MSI Analytical Lab Website&amp;lt;/a&amp;gt;.&amp;lt;/p&amp;gt;</gco:CharacterString>
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                            <gco:CharacterString>The original call for proposals for Emerging Topics in Biogeochemical Cycles (ETBC) was issued in September 2007 by the US NSF Directorate for Geosciences (NSF 07-049).  
The Geosciences Directorate (GEO) is substantially augmenting our past funding sources to explicitly support emerging areas of interdisciplinary research.  We seek to foster transformational advances in our quantitative or mechanistic understanding of biogeochemical cycles that integrate physical-chemical-biological processes over the range of temporal and/or spatial scales in Earthâ€™s environments. We encourage submission of proposals that address emerging topics in biogeochemical cycles, the water cycle or their coupling, across the interfaces of atmosphere, land, and oceans.  Proposals must cross the disciplinary boundaries of two or more divisions in Geosciences (e.g. ATM, EAR, OCE) or of at least one division in Geosciences and a division in another NSF directorate.  
Although funding programmatic disciplines continues to provide a robust and adaptable framework to build our understanding of the geosciences and the earth as a system, there are classes of emerging and challenging problems that require integration of concepts and observations across diverse fields.   Our goal is to enhance such integration. Successful proposals need to develop intellectual excitement in the participating disciplinary communities.  Also encouraged are proposals that have broader educational, diversity, societal, or infrastructure impacts that capitalize on this interdisciplinary opportunity.</gco:CharacterString>
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                            <gco:CharacterString>&lt;p&gt;&lt;strong&gt;NSF Award Abstract: &lt;/strong&gt;&lt;/p&gt;
&lt;p&gt;Coral reefs provide important benefits to society, from food to exceptional biodiversity to shoreline protection and recreation, but they are threatened by natural perturbations and human activities, including those causing global-scale changes. These pressures increasingly are causing coral reefs to undergo large, often abrupt, ecological changes where corals are being replaced by seaweeds or other undesirable organisms. Historically, the major agent of disturbance to coral reefs has been powerful storms, but in recent decades, episodes of mass coral bleaching from marine heat waves have become more frequent and severe as the temperature of ocean surface waters continues to rise. Coral reefs are further stressed by local human activities that cause nutrient pollution and deplete herbivorous fishes that control growth of seaweeds. Studying how coral reefs respond to these two types of disturbance under different levels of nutrient pollution and fishing provides essential information on what affects the ability of coral reefs to buffer environmental change and disturbances without collapsing to a persistent, degraded condition. The fundamental goals of the Moorea Coral Reef Long Term Ecological Research program (MCR LTER) are to understand how and why coral reefs change over time, to assess the consequences of these changes, and to contribute scientific knowledge needed to sustain coral reef ecosystems and the important societal services they provide. This research improves understanding and management of coral reefs, which benefits all groups concerned with the welfare of this ecologically, economically and culturally important ecosystem. In addition to academic communities, scientific findings are communicated to interested individuals, non-governmental organizations, island communities and governmental entities. These findings also are integrated into K-12, undergraduate, graduate and public education activities through a multi-pronged program that includes inquiry-based curricula, interactive and media-based public education programs, and internet-based resources. MCR?s research, training, education and outreach efforts all emphasize broadening participation in STEM fields and strengthening STEM literacy.&lt;/p&gt;
&lt;p&gt;New research activities build on MCR LTER?s powerful foundation of long-term observations and broad ecological understanding of oceanic coral reefs to address the following core issues: How is the changing disturbance regime (recurrent heat waves in addition to cyclonic storms) altering the resilience of coral reefs, and what are the ecological consequences of altered resilience? Research activities are organized around a unifying framework that explicitly addresses how reef communities are affected by the nature and history of coral-killing disturbances, and how those responses to disturbance are influenced by the pattern of local human stressors. New studies answer three focal questions: (1) How do different disturbance types, which either remove (storms) or retain (heat waves) dead coral skeletons, affect community dynamics, abrupt changes in ecological state, and resilience? (2) How do local stressors interact with new disturbance regimes to create spatial heterogeneity in community dynamics, ecosystem processes, and spatial resilience? And (3) What attributes of coral and coral reef communities influence their capacity to remain resilient under current and future environmental conditions? These questions provide an unparalleled opportunity to test hypotheses and advance theory regarding ecological resilience and the causes and consequences of abrupt ecological change, which is broadly relevant across aquatic and terrestrial ecosystems.&lt;/p&gt;
&lt;p&gt;This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.&lt;/p&gt;
&lt;p&gt;&lt;strong&gt;From &lt;a href=&quot;http://www.lternet.edu/sites/mcr/&quot;&gt;http://www.lternet.edu/sites/mcr/&lt;/a&gt; and &lt;a href=&quot;http://mcr.lternet.edu/&quot;&gt;http://mcr.lternet.edu/&lt;/a&gt;&lt;/strong&gt;:&lt;br /&gt;
The Moorea Coral Reef LTER site encompasses the coral reef complex that surrounds the island of Moorea, French Polynesia (17°30'S, 149°50'W). Moorea is a small, triangular volcanic island 20 km west of Tahiti in the Society Islands of French Polynesia. An offshore barrier reef forms a system of shallow (mean depth ~ 5-7 m), narrow (~0.8-1.5 km wide) lagoons around the 60 km perimeter of Moorea. All major coral reef types (e.g., fringing reef, lagoon patch reefs, back reef, barrier reef and fore reef) are present and accessible by small boat.&lt;/p&gt;
&lt;p&gt;The MCR LTER was established in 2004 by the US National Science Foundation (NSF) and is a partnership between the University of California Santa Barbara and California State University, Northridge. MCR researchers include marine scientists from the UC Santa Barbara, CSU Northridge, UC Davis, UC Santa Cruz, UC San Diego, CSU San Marcos, Duke University and the University of Hawaii. Field operations are conducted from the UC Berkeley Richard B. Gump South Pacific Research Station on the island of Moorea, French Polynesia.&lt;/p&gt;
&lt;p&gt;&lt;strong&gt;MCR LTER Data:&lt;/strong&gt; The Moorea Coral Reef (MCR) LTER data are managed by and available directly from the MCR project data site URL shown above.  The datasets listed below were collected at or near the MCR LTER sampling locations, and funded by NSF OCE as ancillary projects related to the MCR LTER core research themes.&lt;/p&gt;
&lt;p&gt;&lt;strong&gt;This project is supported by continuing grants with slight name variations:&lt;/strong&gt;&lt;/p&gt;
&lt;ul&gt;
&lt;li&gt;LTER: Long-Term Dynamics of a Coral Reef Ecosystem&lt;/li&gt;
&lt;li&gt;LTER: MCR II - Long-Term Dynamics of a Coral Reef Ecosystem&lt;/li&gt;
&lt;li&gt;LTER: MCR IIB: Long-Term Dynamics of a Coral Reef Ecosystem&lt;/li&gt;
&lt;li&gt;LTER: MCR III: Long-Term Dynamics of a Coral Reef Ecosystem&lt;/li&gt;
&lt;li&gt;LTER: MCR IV: Long-Term Dynamics of a Coral Reef Ecosystem&lt;/li&gt;
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                            <gco:CharacterString>&lt;p&gt;This project is part of the ETBC (Emerging Topics in Biogeochemical Cycles) program.&lt;/p&gt;
&lt;p&gt;&lt;strong&gt;From NSF award proposal:&lt;/strong&gt;&lt;br /&gt;
The proposed research will investigate the coupling between primary producers and the utilization of dissolved organic matter (DOM) by marine heterotrophic microbes on coral reefs. Previous metagenomic studies of the microbial communities associated with near-pristine and degraded coral reefs demonstrated a shift from a microbial food web similar to the open ocean (&lt;em&gt;Prochlorococcus&lt;/em&gt; spp. and SAR11-like bacteria) to a community dominated by &quot;super-heterotrophs&quot;, most closely related to known pathogens like&lt;em&gt; E. coli&lt;/em&gt;, &lt;em&gt;Staphylococcus&lt;/em&gt; spp., &lt;em&gt;Streptococcus&lt;/em&gt; spp., &lt;em&gt;Enterobacter&lt;/em&gt; spp. and &lt;em&gt;Vibrio&lt;/em&gt; spp. This shift is associated with a decline in coral cover and an increase in coral disease prevalence. Our previous research has also shown that dissolved organic carbon (DOC) concentrations are lower on coral reef platforms compared to measurements of offshore waters (60-80 µM). On degraded reefs, we have observed DOC measurements as low as 30 - 40 µM, a value similar to concentrations observed in the deep Pacific Ocean. The observation of low DOC measurements on degraded reefs is decoupled from the high abundance of macroalgae, which one might expect would raise levels of DOC through the release of photosynthate into the water column.&lt;/p&gt;
&lt;p&gt;The data generated from the proposed research are key to understanding the microbial, chemical, and ecological dynamics on today’s coral reefs. The proposed research plan will consist of five inter-related objectives that will use a combination of field surveys, molecular characterization (microbes and DOM) and experimental approaches to assess the overall hypothesis. We propose to use a combination of archived samples from previous reef expeditions as well as conduct two field visits to reefs in French Polynesia (Moorea) and the Line Islands (Kiritimati) for focused sampling and experimentation. The biogeochemistry, local physical oceanography, and detailed reef ecology has been well characterized and continues to be monitored as part of the MCR-LTER program at Moorea. This environmental context will be useful in interpreting our experimental and field results at Moorea. We will also compare two field sites, Moorea and Kiritimati to ensure that our results are not specific to one region.&lt;/p&gt;</gco:CharacterString>
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	Name: cruiseid
	Units: dimensionless
	Description: &lt;p&gt;Name of the research campaign/cruise/deployment.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/473710.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/473711.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/473713.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/473714.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/473715.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/473717.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/473718.rdf
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http://lod.bco-dmo.org/id/dataset-parameter/473719.rdf
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	Units: decimal degrees
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http://lod.bco-dmo.org/id/dataset-parameter/473720.rdf
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	Units: meters
	Description: &lt;p&gt;Sampling depth in meters.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/473722.rdf
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	Units: 10^8 cells per liter
	Description: &lt;p&gt;Bacterioplankton abundance in 10^8 cells/Liter. Originally named 'BACT'. Methodological reference is Nelson et al. 2011, The ISME Journal.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/473723.rdf
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	Units: cells per milliliter
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	Units: cells per milliliter
	Description: &lt;p&gt;Prochlorococcus abundance in cells/mL by flow cytometry. (2011 data only.)&lt;/p&gt; 
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	Units: cells per milliliter
	Description: &lt;p&gt;Synechococcus abundance in cells/mL by flow cytometry. (2011 data only.)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/473726.rdf
	Name: DOC
	Units: micromolar (uM)
	Description: &lt;p&gt;Dissolved organic carbon concentration by HTCO in micromolar units. Glass fiber filtrate type GF/F (Whatman). Methodological reference is Carlson et al. 2010, DSRII.&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/473727.rdf
	Name: TDN
	Units: micromolar (uM)
	Description: &lt;p&gt;Total dissolved nitrogen by HTCO in micromolar units. Glass fiber filtrate type GF/F (Whatman). (2011 data only.)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/473728.rdf
	Name: NO3
	Units: micromolar (uM)
	Description: &lt;p&gt;Nitrate concentration in micromolar units by flow injection autoanalyzer (Lachat QuikChem 8000; difference of nitrate+nitrite and nitrate). Glass fiber filtrate type GF/F (Whatman). (2010 data only.)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/473729.rdf
	Name: NO2
	Units: micromolar (uM)
	Description: &lt;p&gt;Nitrite concentration in micromolar units by flow injection autoanalyzer (Lachat QuikChem 8000). Glass fiber filtrate type GF/F (Whatman).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/473730.rdf
	Name: NO3_NO2
	Units: micromolar (uM)
	Description: &lt;p&gt;Nitrite+Nitrate concentration in micromolar units by flow injection autoanalyzer (Lachat QuikChem 8000). Glass fiber filtrate type GF/F (Whatman). (2011 data only.)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/473731.rdf
	Name: PO4
	Units: micromolar (uM)
	Description: &lt;p&gt;Ortho-Phosphate concentration in micromolar units by flow injection autoanalyzer (Lachat QuikChem 8000). Glass fiber filtrate type GF/F (Whatman).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/473732.rdf
	Name: silicate
	Units: micromolar (uM)
	Description: &lt;p&gt;Silicate concentration in micromolar units by flow injection autoanalyzer (Lachat QuikChem 8000). Glass fiber filtrate type GF/F (Whatman). (2011 data only.)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/473733.rdf
	Name: NH4
	Units: micromolar (uM)
	Description: &lt;p&gt;Ammonium ion concentration in micromolar units by flow injection autoanalyzer (Lachat QuikChem 8000). Glass fiber filtrate type GF/F (Whatman).&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/473734.rdf
	Name: chl_a_fluor
	Units: micrograms per liter (ug/L)
	Description: &lt;p&gt;Chlorophyll-a concentration by acetone-extracted fluorometry (Turner AU-10). Reference is Smith et al. 1981, Ref. Rep. SIO. Collected on Glass fiber filter type GF/F (Whatman). (2010 data only.)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/473735.rdf
	Name: POC
	Units: micrograms per liter (ug/L)
	Description: &lt;p&gt;Particulate organic carbon measured by combustion analysis (CEC 440HA). Collected on Glass fiber filter type GF/F (Whatman). (2010 data only.)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/473736.rdf
	Name: PON
	Units: micrograms per liter (ug/L)
	Description: &lt;p&gt;Particulate organic nitrogen measured by combustion analysis (CEC 440HA). Collected on Glass fiber filter type GF/F (Whatman). (2010 data only.)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/473737.rdf
	Name: delta_13C
	Units: dimensionless
	Description: &lt;p&gt;Particulate carbon stable isotope ratios by combustion analysis and mass spectometry (Finnigan Delta Plus Advantage in Continuous Flow Mode). Collected on Glass fiber filter type GF/F (Whatman). (2010 data only.)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/473738.rdf
	Name: delta_15N
	Units: dimensionless
	Description: &lt;p&gt;Particulate nitrogen stable isotope ratios by combustion analysis and mass spectometry (Finnigan Delta Plus Advantage in Continuous Flow Mode). Collected on Glass fiber filter type GF/F (Whatman). (2010 data only.)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/473739.rdf
	Name: temp_inc
	Units: degrees C
	Description: &lt;p&gt;Incubation temperature in degrees celsius/centigrade measured via Hach-Lange HQ40D handheld multimeter. Originally named 'DOpH Inc Temp'. (2010 data only.)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/473740.rdf
	Name: time_inc_min
	Units: minutes
	Description: &lt;p&gt;Incubation time in minutes. Originally named 'DOpH Incubation Time'. (2010 data only.)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/474026.rdf
	Name: time_inc_hrs
	Units: hours
	Description: &lt;p&gt;Incubation time in hours. (2011 data only.)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/474027.rdf
	Name: algal_area_O2
	Units: square centimeters (cm^2)
	Description: &lt;p&gt;Estimated algal/coral surface area from specimens used in oxygen/pH change measurements (methodology detailed in Haas et al. 2011 PLoS ONE). Originally named &amp;#039;Surface area DOpH&amp;#039;. (2010 data only.)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/474028.rdf
	Name: weight_g
	Units: grams
	Description: &lt;p&gt;Coral/algal dry weight in grams; methodological reference is Haas et al. 2011 PLoS ONE. (2010 data only.)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/474029.rdf
	Name: algal_area_C
	Units: square centimeters (cm^2)
	Description: &lt;p&gt;Estimated algal/coral surface area from specimens used in DOC release measurements (methodology detailed in Haas et al. 2011 PLoS ONE). Originally named &amp;#039;Surface Area DOC&amp;#039;. (2010 data only.)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/474030.rdf
	Name: O2_umol_L
	Units: micromolar (uM)
	Description: &lt;p&gt;Dissolved oxygen in micromolar units (not salinity corrected) measured via Hach-Lange HQ40D handheld multimeter. Originally named &amp;#039;DO&amp;#039;. (2010 data only.)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/474031.rdf
	Name: pH
	Units: dimensionless
	Description: &lt;p&gt;pH measured via Hach-Lange HQ40D handheld multimeter. (2010 data only.)&lt;/p&gt; 
http://lod.bco-dmo.org/id/dataset-parameter/474032.rdf
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Sampling from small boats occurred along transects at the Moorea Coral Reef LTER sites during September 2010 and September 2011. Benthic producers were collected from water depths of 1.0 to 1.5 meters at back and fringe reef locations. Bacterioplankton abundance was determined using flow cytometry (Nelson et al. 2011). DOC was measured by a high-temperature TOC analyzer according to methods of Carlson et al. (2010). To assess DOC release by primary producers, each benthic species was incubated using the method of Herndl and Velimirov (Haas et al. 2011). To determine if DOC released by primary producers affects mirobial populations, ~48 hour dark dilution culture incubations were performed to measure changes in concentration of DO, DOC, and bacterioplankton over time (Haas et el. 2011).&amp;lt;/p&amp;gt;

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Bacterioplankton flow cytometry methods: Nelson et al. 2011.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Experimental designs, dissolved oxygen, pH, temperature, and specimen surface area methods: Haas et al. 2011,&amp;amp;nbsp;Herndl &amp;amp;amp; Velimirov B. 1986.&amp;lt;/p&amp;gt;

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&amp;lt;p&amp;gt;Chlorophyll method: Smith et al. 1981.&amp;lt;/p&amp;gt;

&amp;lt;p&amp;gt;Dissolved inorganic nutrients, particulate organic nutrients, and isotope ratios methodological references, calibrations, precision, and accuracy are detailed at the &amp;lt;a href=&amp;quot;http://www.msi.ucsb.edu/services/analytical-lab&amp;quot; target=&amp;quot;_blank&amp;quot;&amp;gt;UCSB MSI Analytical Lab Website&amp;lt;/a&amp;gt;.&amp;lt;/p&amp;gt;</gco:CharacterString>
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